Animal Reproduction (AR)
https://animal-reproduction.org/article/5b5a602ef7783717068b45f8
Animal Reproduction (AR)
Original Article

Effects of BMP-4 and FSH on growth, morphology and mRNA expression of oocyte-secreted factors in cultured bovine secondary follicles

R.O.D.S. Rossi, A.M.L.R. Portela, J.R.S. Passos, E.V. Cunha, A.W.B. Silva, J.J.N. Costa, M.V.A. Saraiva, M.A.M. Donato, C.A. Peixoto, R. Van Den Hurk, J.R.V. Silva

Downloads: 0
Views: 883

Abstract

This study evaluated the effect of different concentrations of bone morphogenetic protein-4 (BMP4), as well as the interaction of BMP-4 and follicle stimulating hormone (FSH) on growth, ultrastructural integrity, and expression of mRNA for growth differentiation factor-9 (GDF-9), BMP-15, maternal antigen that the embryo requires (Mater) and nucleoplasmin-2 (Npm-2) in bovine secondary follicles cultured in vitro for 18 days. Follicles cultured in the presence of 50 ng/ml BMP-4 had a progressive increase in their diameters with the increase of culture period from 0 to 6 and 12 days, but no significant differences were observed among treatments. The presence of both FSH and BMP-4 in a culture medium did not stimulate follicle growth when compared to the control medium. After 12 days, the percentage of normal follicles was maintained similar to that of day 0 in the medium supplemented with both FSH and BMP-4, but no significant differences among treatments were observed after 18 days of culture. BMP-4 maintained the ultrastructural integrity of follicles after 18 days of culture, while follicles cultured in medium supplemented with FSH or both BMP-4 and FSH had oocyte with irregular zona pellucida, vesicular bodies, and an abundance of vacuoles. Follicles cultured in the presence of BMP-4 had an increase in the levels of BMP-15 mRNA, when compared to those cultured in medium supplemented with FSH alone. In conclusion, the addition of BMP-4 in culture medium contributes to preserve follicular ultrastructure, but BMP-4 did not interact positively with FSH. Regarding secondary follicles cultured in the presence of FSH, BMP-4 increases the expression of mRNA for BMP-15

Keywords

BMP-4, BMP-15, GDF-9, cow, culture, secondary follicles
5b5a602ef7783717068b45f8 animreprod Articles
Links & Downloads

Anim Reprod

Share this page
Page Sections