The objective of this study was to assess the effect of several solutions and incubation times in the hypoosmotic swelling test in predicting the in vitro quality of the frozen/thawed semen of Piau boars. Four samples of frozen semen from five different Piau boars were used. For the assessment of the hypoosmotic test, three incubation times were used (5, 30 and 60 min) in three different hypoosmotic solutions (BTS at 75 mOsm/Kg, sucrose at 100 mOsm/Kg and fructose and sodium citrate at 100 mOsm/Kg), thus, samples were submitted to each hypoosmotic solution in three incubation times. The means of sperm motility, spermatic vigor and live sperm by supravital staining for the assessed samples were, respectively, 37.5 ± 7.2%, 2.8 ± 0.3 and 37.4 ± 7.8% in thawing and 15.5 ± 7.6%, 1.7 ± 0.5 and 22.6 ± 6.6% after 120 min of thermoresistance test (P < 0.05). There was no difference between the mean values obtained for reactive sperm in the different incubation times and hypoosmotic solutions, and also no interaction between time and solutions. The results obtained in the present study demonstrated that the hypoosmotic test can be used in assessing the quality of frozen/thawed semen, besides offering alternatives for hypoosmotic solutions and different incubation times in performing the hypoosmotic test to predict the sperm plasmatic membrane quality in boars.