Effect of ovarian tissue transportation in Amburana cearensis extract on the morphology and apoptosis of goat preantral follicles
B.B. Gouveia, V.R.P. Barros, R.J.S. Gonçalves, R.S. Barberino, V.G. Menezes, T.L.B. Lins, T.J.S. Macedo, J.M.S. Santos, L.A. Rolim, P.J. Rolim Neto, J.R.G.S. Almeida, M.H.T. Matos
Anim Reprod, vol.12, n2, p.316-323, 2015
The aim of the present study was to evaluate the effect of Amburana cearensis extract during caprine ovarian tissue transportation on the survival of preantral follicles in vitro. HPLC was used to determine the fingerprint chromatogram of the ethanolic extract. Five goat ovarian pairs were divided into fragments. One fragment was fixed for histology and TUNEL analysis (fresh control). The other fragments were placed in MEM or A. cearensis extract (0.1; 0.2 or 0.4 mg/ml) and stored at 4o C for 6, 12 or 24 h. Preserved fragments were also fixed for histology and TUNEL analysis. The presence of phenolic compounds (protocatechuic acid, epicatechin, p-coumaric acid, gallic acid and kaempferol) in the extract was confirmed using HPLC. The percentage of normal follicles preserved in 0.2 mg/ml A. cearensis for 6 h was similar to that observed in the fresh control. Moreover, the percentage of normal follicles was higher after preservation in 0.2 mg/ml A. cearensis for 6 h than the other A. cearensis treatments and similar to that found in MEM. There were no differences in the percentage of apoptotic cells between fresh control and those preserved for 6 h in MEM or 0.2 mg/ml A. cearensis. In conclusion, both 0.2 mg/ml A. cearensis or MEM can be used for the preservation of goat preantral follicles for up to 6 h. The use of A. cearensis is recommended due to the higher cost of MEM.
caprine, HPLC, medicinal plant, oocyte, preservation, TUNEL