Numerous reproductive biotechnologies are commonly employed to enhance animal production mainly through multiplying animals with high-quality traits in a large-scale production system. There is, however, several peculiarities during the process of embryo in vitro production that are still in need of further studies in order to obtain a higher efficiency. This present review discuss some of such particularities, as well as new models of embryo and gamete production, which will probably be part of a new era of reproductive biotechnologies in a near future