Animal Reproduction (AR)
https://animal-reproduction.org/article/5b5a604ff7783717068b46b4
Animal Reproduction (AR)
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Insemination of four cows per dose of frozen semen with a fixed-time artificial insemination protocol

E.C. Silveira, L.A. Bortolloti, F. Morotti, K.C. Silva-Santos, G.M.G. Santos, E.R. Andrade, M.M. Seneda

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Abstract

The aim of the study was to evaluate the practicality of a 1:4 dilution of doses of semen in fixedtime artificial insemination (FTAI) programs. Nelore cows (n = 803) were synchronized by a conventional FTAI protocol. For AI in the diluted group (n = 392), 0.5 ml semen straws were thawed and 0.5 ml of extender containing amino acids and methylxanthine derivatives was added. The straws were fractionated into four straws of 0.25 ml each (dilution 1:4) with approximately 2.5 x 106 spermatozoa each and the insemination was performed in the ipsilateral horn to the ovary containing the dominant follicle. In the control group (n = 411), AI was performed in the uterine body with one straw of 0.5 ml (10 x 106 spermatozoa) containing semen from the same bull and batch. The conception rate was 49.2% (193/392) for the diluted group and 50.1% (206/411) for the control group, with 1.97 and 0.50 pregnancies per dose of semen, respectively. The dilution of semen provided a pregnancy index similar to the control group; however, the technique increased the number of pregnancies per dose, allowing for the best use of semen of high genetic value and reducing the cost per pregnancy

Keywords

bull, fractionation, FTAI, pregnancy, sperm dose
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