The aim of the study was to evaluate the practicality of a 1:4 dilution of doses of semen in fixedtime artificial insemination (FTAI) programs. Nelore cows (n = 803) were synchronized by a conventional FTAI protocol. For AI in the diluted group (n = 392), 0.5 ml semen straws were thawed and 0.5 ml of extender containing amino acids and methylxanthine derivatives was added. The straws were fractionated into four straws of 0.25 ml each (dilution 1:4) with approximately 2.5 x 106 spermatozoa each and the insemination was performed in the ipsilateral horn to the ovary containing the dominant follicle. In the control group (n = 411), AI was performed in the uterine body with one straw of 0.5 ml (10 x 106 spermatozoa) containing semen from the same bull and batch. The conception rate was 49.2% (193/392) for the diluted group and 50.1% (206/411) for the control group, with 1.97 and 0.50 pregnancies per dose of semen, respectively. The dilution of semen provided a pregnancy index similar to the control group; however, the technique increased the number of pregnancies per dose, allowing for the best use of semen of high genetic value and reducing the cost per pregnancy