The aim of this study was to compare in vitro survival rates of in vivo and in vitro-produced bovine embryos by slow freezing or solid surface vitrification. In vivo-produced blastocysts (n = 210) and in vitroproduced blastocysts (n = 445) were randomly allocated in two cryopreservation groups. Group 1 - embryos were exposed to 1.5 M ethylene glycol (EG) for 5 min, loaded in 0.5 ml straws, frozen at -6.5ºC and seeded. After 10 min of equilibration, straws were cooled at -0.6ºC/min until - 35ºC, and then plunged into liquid nitrogen (-196ºC). Group 2 - embryos were exposed to a 15% EG + 0.25 M trehalose solution for 1 min and then a 30% EG + 1 M trehalose solution for 30 sec to be vitrified using the Cryologic Vitrification Method (CVM®). After at least one week of storage, embryos in the slow freezing group were thawed in a water bath at 30°C for 12 sec and then placed in holding medium for 5 min and transferred into SOF culture media. Vitrified embryos were placed directly into a 0.25 M sucrose solution for 5 min then cultured in SOF medium. Re-expansion and hatching rates were evaluated at 24 and 72 h, respectively. In vivo-produced embryos had higher (P < 0.01) re-expansion (179/210, 81% vs. 244/445, 54%) and hatching rates (159/210, 72% vs. 177/445, 39%) than in vitro-produced embryos, regardless of the cryopreservation method. However, re-expansion and hatching rates were higher (P < 0.01) for in vitro-produced vitrified embryos (155/223, 69% and 132/223, 59%) than in vitro-produced embryos cryopreserved by slow freezing (89/222, 40% and 45/222, 20%). Although similar re-expansion rates were obtained with in vivoproduced embryos cryopreserved by the two systems, hatching rates tended to be lower (P = 0.09) with in vivo-produced embryos that were vitrified as compared to slow freezing. In conclusion, solid surface vitrification improved the cryosurvival rates of in vitroproduced embryos compared to the conventional slow freezing procedure.