This study was designed to investigate the effect of supplementation with 5 or 10% fetal calf serum (FCS) on bovine embryo development in three types of culture media added with amino acids (aa): Charles Rosenkrans 2 medium (CR2aa), potassium simplex optimized medium (KSOMaa) and synthetic oviduct fluid (SOFaa). In vitro matured (IVM) and fertilized (IVF) oocytes were in vitro cultured (IVC) from Day 1 to 10 of development. The increase in FCS in CR2aa media did not affect embryo development and hatching. However, the increase from 5 to 10% FCS in KSOMaa and SOFaa induced a decrease in the number of blastocysts. Embryo hatching was similar for embryos cultured with 5 or 10% FCS in both media. We concluded that a lower concentration of FCS increased embryo development rate in SOFaa and KSOMaa media and that there was no detrimental effect of high FCS concentration in CR2aa.