This study consisted of two experiments. In Experiment 1, in vitro semen quality was assessed by comparing swine semen conditioned with either PIGPEL-5 extender at 5ºC or BTS extender at 17°C. Sperm motility was similar (P > 0.05) at 24 and 48 h post-dilution for PIGPEL-5 (67.9% and 59.3%, respectively) and BTS (73.9% and 64.0%, respectively). The frequency of sperm vigor equal to 4 was higher for PIGPEL-5 (P < 0.05) than for BTS after 24 h (70.8% and 29.2%, respectively) and 48 h (87.9% and 12.1%, respectively). After a thermal stress test, the frequencies of sperm vigor equal to 3 and 4 were higher (P < 0.05) for PIGPEL-5 than for BTS in both periods, but motility was not different between extenders (P > 0.05) in any period. Acrosome integrity was lower (P < 0.05) for PIGPEL-5 than for BTS in both periods, but head and tail morphology did not differ (P > 0.05). In the hypoosmotic swelling test, the frequency of tail rolling or bending was higher (P < 0.05) for BTS than for PIGPEL-5 after 24 h (33.1% and 8.2%, respectively) and 48 h (24.3% and 7.2%, respectively). In Experiment 2, 60 pre-pubertal gilts were artificially inseminated following induction of ovulation and using semen conditioned with both extenders (n = 30, per extender). There was no difference (P > 0.05) in the number of recovered or fertilized oocytes or in the fertilization rate for BTS (6.6 ± 0.6, 6.0 ± 0.6, and 83.7% ± 4.4, respectively) and for PIGPEL-5 (4.6 ± 0.9, 4.3 ± 0.8, and 87.3% ± 6.3, respectively). These results indicate that the PIGPEL-5 extender can be used to condition swine semen at 5ºC because parameters of semen quality and in vivo fertility following its use were generally similar to those of a conventional extender at 17°C.