Animal Reproduction (AR)
Animal Reproduction (AR)
Original Article

The use of coconut water solution (Cocos nucifera) as a holding medium for immature bovine oocytes for in vitro embryo production

M.S. Cordeiro, E.H.S. Silva, M.S. Miranda, F.C. Biondi, S.S.D. Santos, O.M. Ohashi

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The aim of this study was to evaluate a coconut-water-based holding medium for preserving immature bovine cumulus-oocyte complexes (COCs). The selected COCs were grouped as follows: Control Group – immediately-matured COCs; Groups H6, H9, and H12 - COCs preserved in TCM-199 + Hepes for 6, 9, and 12 h, respectively; and Groups C6, C9, and C12 - COCs preserved in the holding coconut solution for 6, 9, and 12 h, respectively. The total in vitro maturation (IVM) time of the experimental groups was 24 h, that is, preservation for 6, 9, and 12 h and IVM for 18, 15, and 12 h, respectively. After in vitro fertilization and embryo culture, cleavage, blastocyst, and hatched blastocyst rates were analyzed. A decrease was observed (P < 0.05) in blastocyst rates in Groups H9 (33.9 ± 8.7%), H12 (34.2 ± 6.0%), C9 (35.9 ± 8.9%), and C12 (35.6 ± 5.6%) when compared to the Control (50.9 ± 8.7%), H6 (49.7 ± 7.3%), and the C6 Groups (49.4 ± 4.1%). The results indicate that the coconutwater holding solution can be used as a medium to preserve immature bovine COCs for up to 6 h with no effects on embryo production.


coconut water, oocyte, in vitro embryo production
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