Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.1590/1984-3143-AR2020-0431
Animal Reproduction (AR)
ORIGINAL ARTICLE

Altrenogest during early pregnancy modulates uterine glandular epithelium and endometrial growth factor expression at the time implantation in pigs

Bruno Bracco Donatelli Muro; Diego Feitosa Leal; Rafaella Fernandes Carnevale; Mariana Andrade Torres; Maitê Vidal Mendonça; Denis Hideki Nakasone; Cristian Hernando Garcia Martinez; Gisele Mouro Ravagnani; Matheus Saliba Monteiro; André Pegoraro Poor; Simone Maria Massami Kitamura Martins; Priscila Viau; Cláudio Alvarenga de Oliveira; Raquel Vasconcelos Guimarães de Castro; Brendon Willian Bessi; Fabiana Fernandes Bressan; Lidia Hildebrand Pulz; Ricardo Francisco Strefezzi; Glen William Almond; André Furugen Cesar de Andrade

Downloads: 0
Views: 33

Abstract

This study evaluated the effects of supplying altrenogest from day 6-12 of pregnancy on the endometrial glandular epithelium, corpora lutea (CL) morphology, and endometrial and CL gene expression. A total of 12 crossbred females (Landrace × Large White) were used. The females were assigned to 4 treatments according to a random design with a 2 × 2 factorial arrangement, with two categories (sow or gilt) and two treatments (non-treated and treated with altrenogest). On day 6 of pregnancy, animals were allocated to one of the following groups: non-treated (NT, n = 6; 3 sows and 3 gilts), and (T, n = 6; 3 sows and 3 gilts) treated daily with 20 mg of altrenogest, from day 6-12 of pregnancy. All animals were euthanized on day 13 of pregnancy. All CLs were individually weighed, and their volume were determined. The endometrial glandular density (GD), mean glandular area (MGA), and vascular density (VD) were determined by histomorphometric and immunohistochemical analyses. Endometrium samples were collected and analyzed by qRT-PCR to evaluate the abundance of transcripts for VEGF and IGF-I. Females in the T group had higher MGA (P < 0.05) compared to the NT group. There was no effect of treatment on GD or VD for both experimental groups. Sows in the T group had augmented expression of IGF-I (P < 0.05). Progestagen had no detrimental effect on CL morphology. In conclusion, altrenogest improves the uterine environment during the peri-implantation period in pigs without compromising corpora lutea development.

Keywords

corpus luteum, endometrium, gilts, IGF-I, progesterone

References

Bailey DW, Dunlap KA, Frank JW, Erikson DW, White BG, Bazer FW, Burghardt RC, Johnson GA. Effects of long-term progesterone on developmental and functional aspects of porcine uterine epithelia and vasculature: progesterone alone does not support development of uterine glands comparable to that of pregnancy. Reproduction. 2010;140(4):583-94. http://dx.doi.org/10.1530/REP-10-0170. PMid:20634388.

Bazer FW, Song G, Kim J, Dunlap KA, Satterfield MC, Johnson GA, Burghardt RC, Wu G. Uterine biology in pigs and sheep. J Anim Sci Biotechnol. 2012;3(1):23. http://dx.doi.org/10.1186/2049-1891-3-23. PMid:22958877.

Brüssow K-P, Schneider F, Nürnberg G. Alteration of gonadotrophin and steroid hormone release, and of ovarian function by a GnRH antagonist in gilts. Anim Reprod Sci. 2001;66(1-2):117-28. http://dx.doi.org/10.1016/S0378-4320(01)00093-8. PMid:11343847.

Chen X, Li A, Chen W, Wei J, Fu J, Wang A. Differential gene expression in uterine endometrium during implantation in pigs. Biol Reprod. 2015;92(2):53. http://dx.doi.org/10.1095/biolreprod.114.123075. PMid:25519183.

Forde N, Carter F, Fair T, Crowe MA, Evans ACO, Spencer TE, Bazer FW, McBride R, Boland MP, O’Gaora P, Lonergan P, Roche JF. Progesterone-regulated changes in endometrial gene expression contribute to advanced conceptus development in cattle. Biol Reprod. 2009;81(4):784-94. http://dx.doi.org/10.1095/biolreprod.108.074336. PMid:19553605.

Geisert R, Schmitt RAM. Early embryonic survival in the pig: can it be improved? J Anim Sci. 2002;80(Suppl 1):E54-65.

Geisert RD, Chamberlain CS, Vonnahme KA, Spicer LJ. Possible role of kallikrein in proteolysis of insulin-like growth factor binding proteins during the oestrus cycle and early pregnancy in pigs. Reproduction. 2001;121(5):719-28. http://dx.doi.org/10.1530/rep.0.1210719. PMid:11427159.

Jeong W, Song G, Bazer FW, Kim J. Insulin-like growth factor I induces proliferation and migration of porcine trophectoderm cells through multiple cell signaling pathways, including protooncogenic protein kinase 1 and mitogen-activated protein kinase. Mol Cell Endocrinol. 2014;384(1-2):175-84. http://dx.doi.org/10.1016/j.mce.2014.01.023. PMid:24508636.

Kridli RT, Khalaj K, Bidarimath M, Tayade C. Placentation, maternal-fetal interface, and conceptus loss in swine. Theriogenology. 2016;85(1):135-44. http://dx.doi.org/10.1016/j.theriogenology.2015.08.001. PMid:26324112.

Lewis AM, Kaye PL, Lising R, Cameron RDA. Stimulation of protein synthesis and expansion of pig blastocysts by insulin in vitro. Reprod Fertil Dev. 1992;4(1):119-23. http://dx.doi.org/10.1071/RD9920119. PMid:1585007.

Muro BBD, Carnevale RF, Leal DF, Torres MA, Mendonça MV, Nakasone DH, Martinez CHG, Ravagnani GM, Monteiro MS, Poor AP, Martins SMMK, Viau P, Oliveira CA, Pulz LH, Strefezzi RF, Almond GW, de Andrade AFC. Supplemental progesterone during early pregnancy exerts divergent responses on embryonic characteristics in sows and gilts. Animal. 2020;14(6):1234-40. http://dx.doi.org/10.1017/S1751731119002982. PMid:31907084.

Mesquita FS, Pugliesi G, Scolari SC, França MR, Ramos RS, Oliveira M, Papa PC, Bressan FF, Meirelles FV, Silva LA, Nogueira GP, Membrive CMB, Bineli M. Manipulation of the periovulatory sex steroidal milieu affects endometrial but not luteal gene expression in early diestrus nelore cows. Theriogenology. 2014;81(6):861-69. https://doi.org/10.1016/j.theriogenology.2013.12.022. PMid:24507960.

Machado LS, Pieri NCG, Botigelli RC, Castro RVG, Souza AF, Bridi A, Lima MA, Neto PF, Pessôa LVF, Martins SMMK, Andrade AFC, Freude KK, Bressan FF. Generation of neural progenitor cells from porcine-induced pluripotent stem cells. J Tissue Eng Regen Med. 2020;14(12):1880-91. https://doi.org/10.1002/term.3143 https://doi.org/10.1002/term.3143 PMid:33049106.

Okrasa S, Franczak A, Zmijewska A, Wojciechowicz B, Dziekonski M, Martyniak M, Kalakowska J, Zglejc K, Kotwica G. The uterine secretory activity and its physiological changes in the pig. Acta Biol Crac. 2014;55-56:40-57.

Prophet EB, Mills B, Arrington J, Sobin LH. Laboratory methods in histotechnology. Washington: Armed Forces Institute of Pathology; 1992. 279 p.

Pugliesi G, Santos FB, Lopes E, Nogueira É, Maio JR, Binelli M. Improved fertility in suckled beef cows ovulating large follicles or supplemented with long-acting progesterone after timed-AI. Theriogenology. 2016;85(7):1239-48. http://dx.doi.org/10.1016/j.theriogenology.2015.12.006. PMid:26764150.

Ravagnani GM, Martinez CHG, Carnevale RF, Muro BBD, Mendonça MV, Pavaneli APP, Passareli MS, Nakasone DH, Strefezzi RF, Martins SMMK, Andrade AFC. Effects of oestrus synchronization with altrenogest in gilts on endometrial and embryonic characteristics. Animal. 2020;14(9):1899-905. http://dx.doi.org/10.1017/S1751731120000658. PMid:32290879.

Simmen RCM, Simmen FA, Hofig A, Farmer SJ, Bazer FW. Hormonal regulation of insulin-like growth factor gene expression in pig uterus. Endocrinology. 1990;127(5):2166-74. http://dx.doi.org/10.1210/endo-127-5-2166. PMid:2171908.

Sinowatz F, Friess AE. Uterine glands of the pig during pregnancy: an ultrastructural and cytochemical study. Anat Embryol. 1983;166(1):121-34. http://dx.doi.org/10.1007/BF00317948. PMid:6837929.

Soede NM, Bouwman E, van der Laan I, Hazeleger W, Jourquin J, Langendijk P, Kemp B. Progestagen supplementation during early pregnancy does not improve embryo survival in pigs. Reprod Domest Anim. 2012;47(5):835-41. http://dx.doi.org/10.1111/j.1439-0531.2011.01977.x. PMid:22229702.

Spencer TE, Gray A, Johnson GA, Taylor KM, Gertler A, Gootwine E, Ott TL, Bazer FW. Effects of recombinant ovine interferon tau, placental lactogen, and growth hormone on the ovine uterus. Biol Reprod. 1999;61(6):1409-18. http://dx.doi.org/10.1095/biolreprod61.6.1409. PMid:10569983.

Szafranska B., Ziecik A. Active and passive immunization against luteinizing hormone in pigs. Acta Physiol Hung. 1989;74(3-4):253-258. PMid:2640407.

Waclawik A, Kaczmarek MM, Blitek A, Kaczynski P, Ziecik AJ. Embryo-maternal dialogue during pregnancy establishment and implantation in the pig. Mol Reprod Dev. 2017;84(9):842-55. http://dx.doi.org/10.1002/mrd.22835. PMid:28628266.

Wang J, Feng C, Liu T, Shi M, Wu G, Bazer FW. Physiological alterations associated with intrauterine growth restriction in fetal pigs: causes and insights for nutritional optimization. Mol Reprod Dev. 2017;84(9):897-904. http://dx.doi.org/10.1002/mrd.22842. PMid:28661576.

Welter H, Wollenhaupt K, Tiemann U, Einspanier R. Regulation of the VEGF-system in the endometrium during eteroid-replacement and early pregnancy of pigs. Exp Clin Endocrinol Diabetes. 2003;111(1):33-40. http://dx.doi.org/10.1055/s-2003-37498. PMid:12605348.

Young KH, Kraeling RR, Bazer FW. Effect of pregnancy and exogenous ovarian steroids on endometrial prolactin receptor ontogeny and uterine secretory response in pigs. Biol Reprod. 1990;43(4):592-9. http://dx.doi.org/10.1095/biolreprod43.4.592. PMid:2289013.

Ziecik AJ, Przygrodzka E, Jalali BM, Kaczmarek MM. Regulation of the porcine corpus luteum during pregnancy. Reproduction. 2018;156(3):R57-67. http://dx.doi.org/10.1530/REP-17-0662. PMid:29794023.
 


Submitted date:
10/20/2020

Accepted date:
04/20/2021

60ae9428a95395567c2ffa76 animreprod Articles
Links & Downloads

Anim Reprod

Share this page
Page Sections