Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.1590/1984-3143-AR2020-0554
Animal Reproduction (AR)
Original Article

Subdose of human chorionic gonadotropin applied at the Hou Hai acupoint on follicular dynamics and luteal development in donkeys

Márcio de Oliveira Ribeiro; Rodrigo Freitas Bittencourt; Marcus Antônio Rossi Feliciano; Ana Lúcia Almeida Santana; Mariana Alves de Andrade Silva; Morgana Duarte Felix; Larissa Rodrigues Santana; Larissa Pires Barbosa

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Abstract

Abstract: The objective of this study was to evaluate the effects of an hCG subdose applied at the Hou Hai acupoint as an ovulation inducer in donkeys. Eleven donkeys were distributed in randomized blocks in T1 = application of 1,500 IU of hCG intravenous (IV); T2 = 450 IU of hCG applied at the false acupoint (IV), and T3 = 450 IU of hCG applied at the Hou Hai acupoint. There was no difference (P > 0.05) between the treatments regarding the mean diameter of the pre-ovulatory follicle (34.5 ± 1.3 mm), the ovulation rate (96.97%), the interval between induction and ovulation (58.07 ± 16.82 h), the mean diameter of the CL (D0 = 23.0 ± 0.6; D2 = 27.7 ± 1.9 and D8 = 28.2 ± 0.8mm), and serum P4 concentrations (10.50 ± 2.99 ng.mL-1). The application of 450 IU of hCG at the Hou Hai acupoint increased ovulation rate (72.73%) more than 48 h after induction (P = 0.03) and a larger diameter of the CL on D4 (30.7 ± 5.1 mm) (P = 0.04). The vascularization area of the CL on D8, obtained by minimum number of colored pixel (NCP), was greater (P < 0.05) in the donkeys that received 1,500 IU of IV hCG (T1, 41.91 ± 1.17), and we found a positive correlation (P < 0.05) between mean NCP and P4 concentration in the donkeys that received 450 IU of hCG IV at the false acupoint (T2) or at the Hou Hai acupoint (T3). The application of 450 IU of hCG by IV route at the false acupoint or the Hou Hai acupoint was sufficient to induce ovulation in donkeys, demonstrating that the average dosage commonly used for this species is too high.

Keywords

acupuncture, donkey, doppler, progesterone, pêga race

References

Arruda RP, Visintin JA, Fleury JJ, Garcia AR, Madureira EH, Celeghini ECC, Neves Neto JR. Existem relações entre tamanho e morfoecogenecidade do corpo lúteo detectados pela ultra-som e os teores de progesterona plasmática em receptoras de embriões equinos? Braz J Vet Res Anim Sci. 2001;38(5):233-9. http://dx.doi.org/10.1590/S1413-95962001000500007.

Awan FS, Mehmood MU, Sattar A, Ahmad N. Comparative efficacy of hCG or GnRH analogue (lecirelin acetate) on follicular dynamics, degree of endometrial edema, sexual behavior, ovulation and pregnancy rate in crossbred broodmares. J Equine Vet Sci. 2016;41:71-2. http://dx.doi.org/10.1016/j.jevs.2016.04.062.

Cardoso RC, Barbosa LP, Souza RS, França CS, Ribeiro MDM Jr, Santana ALA, Jesus RDL, Santos RS. Application of hormonal subdoses at acupoint Hou Hai in estrus synchronization protocols of goats. Semina. Ciênc Agr. 2018;39(3):1135-42. http://dx.doi.org/10.5433/1679-0359.2018v39n3p1135.

Carluccio A, Panzani S, Tosi U, Faustini M, De Amicis I, Veronesi MC. Efficacy of hCG and GnRH for inducing ovulation in the jenny. Theriogenology. 2007;68(6):914-9. http://dx.doi.org/10.1016/j.theriogenology.2007.07.005. PMid:17716724.

Carluccio A, Panzani S, Contri A, Tosi U, De Amicis I, Veronesi MC. Luteal function in jennies following PGF2α treatment 3 days after ovulation. Theriogenology. 2008;70(1):121-5. http://dx.doi.org/10.1016/j.theriogenology.2008.03.010. PMid:18439666.

Carluccio A, Gloria A, Robbe D, Veronesi MC, De Amicis I, Cairoli F, Contri A. Reproductive characteristics of foal heat in female donkeys. Animal. 2017;11(3):461-5. http://dx.doi.org/10.1017/S175173111600183X. PMid:27571706.

Conceição JC, Freitas LM No, Aguiar CR Fo, Araújo GHM, Oliveira JV, Bartolomeu CC, Oba E, Meira C. Concentração plasmática de progesterona durante o ciclo estral de Jumentas (Equus asinus) com ovulações simples e duplas. Med Vet. 2009;3(3):15-9.

Cuervo-Arango J, Newcombe JR. Repeatability of preovulatory follicular diameter and uterine edema pattern in two consecutive cycles in the mare and how they are influenced by ovulation inductors. Theriogenology. 2008;69(6):681-7. http://dx.doi.org/10.1016/j.theriogenology.2007.11.019. PMid:18242672.

Derar RI, Hussein HA. Ovarian follicular dynamics during the estrous cycle in jennies in upper egypt. Vet Med Int. 2011;1:1-6. http://dx.doi.org/10.4061/2011/860518. PMid:21647342.

Díaz-Duran M, Zarco L, Boeta AM. Ovarian dynamics and estrous cycle length in the donkey (Equus asinus). Theriogenology. 2017;103:1-8. http://dx.doi.org/10.1016/j.theriogenology.2017.07.003. PMid:28763724.

Galisteo J, Perez-Marin CC. Factors affecting gestation length and estrus cycle characteristics in Spanish donkey breeds reared in southern Spain. Theriogenology. 2010;74(3):443-50. http://dx.doi.org/10.1016/j.theriogenology.2010.02.027. PMid:20451997.

Gastal EL, Gastal MO, Ginther OJ. Relationships of changes in B-mode echotexture and colour-Doppler signals in the wall of the preovulatory follicle to changes in systemic oestradiol concentrations and the effects of human chorionic gonadotrophin in mares. Reproduction. 2006;131(4):699-709. http://dx.doi.org/10.1530/rep.1.01011. PMid:16595721.

Ginther OJ, Gastal EL, Gastal MO. Spatial relationships between serrated granulosa and vascularity of the preovulatory follicle and developing corpus luteum. J Equine Vet Sci. 2007;27(1):20-7. http://dx.doi.org/10.1016/j.jevs.2006.11.012.

Grizendi BM, Fernandes CB. Éguas em idade avançada: perda embrionária relacionada à deficiência de progesterona e à doença endometrial. Rev Acad Ciênc Anim. 2015;13(1):23-9. http://dx.doi.org/10.7213/academica.13.FC.AO02.

Hwang YC, Limehouse JB. Atlas de acupuntura canina. In: Schoen AM, editor. Acupuntura veterinária: da arte antiga à moderna. São Paulo: ROCA; 2006. p. 122-146.

Köhne M, Kuhl J, Ille N, Erber R, Aurich C. Treatment with human chorionic gonadotrophin beforeovulation increases progestin concentration inearly equine pregnancies. Anim Reprod Sci. 2014;149(3-4):187-93. http://dx.doi.org/10.1016/j.anireprosci.2014.07.002. PMid:25096723.

Luna SPL, Angeli AL, Ferreira CL, Lettry V, Scognamillo-Szabo M. Comparison of pharmacopuncture, aquapuncture and acepromazine for sedation of horses. Evid Based Complement Alternat Med. 2008;5(1):267-72. http://dx.doi.org/10.1093/ecam/nel096. PMid:18830446.

Mariante SA, Egito AA. Animal genetic resources in Brazil: Result of five centuries of natural selection. Theriogenology. 2002;57(1):223-35. http://dx.doi.org/10.1016/S0093-691X(01)00668-9. PMid:11775972.

Morel MCGD, Newcombe JR, Hayward K. Factors affecting pre-ovulatory follicle diameter in the mare: the effect of mare age, season and presence of other ovulatory follicles (multiple ovulation). Theriogenology. 2010;74(7):1241-7. http://dx.doi.org/10.1016/j.theriogenology.2010.05.027. PMid:20615543.

Nagy P, Huszenicza G, Reiczigel J, Juhász J, Kulcsár M, Abaváry K, Guillaume D. Factors affecting plasma progesterone concentration and the retrospective determination of time of ovulation in cyclic mares. Theriogenology. 2004;61(2-3):203-14. http://dx.doi.org/10.1016/S0093-691X(03)00211-5. PMid:14662122.

Quaresma M, Payan-Carreira R. Characterization of the estrous cycle of Asinina de Miranda jennies (Equus asinus). Theriogenology. 2015;83(4):616-24. http://dx.doi.org/10.1016/j.theriogenology.2014.10.021. PMid:25467767.

Rodrigues TG, Silva FS, Fagundes B, Estadual U, Fluminense N, Igua UN. Uso da eCG para formação do corpo lúteo eqüino e produção de progesterona. Acta Biomed Bras. 2014;5(1):56-70.

Romano RM, Ferreira JC, De Siqueira CH, Boakari YL, Ignácio FS, Novaes Filho LF, Thompson DL Jr, Meira C. Characterization of luteal blood flow and secretion of progesterone in mares treated with human chorionic gonadotropin for ovulation induction or during early diestrus. J Equine Vet Sci. 2015;35(7):591-7. http://dx.doi.org/10.1016/j.jevs.2015.03.196.

Samper JC. Induction of estrus and ovulation: why some mares respond and others do not. Theriogenology. 2008;70(3):445-7. http://dx.doi.org/10.1016/j.theriogenology.2008.04.040. PMid:18554709.

Sieme H, Oldenho FH, Wolkers WF. Mode of action of cryoprotectants for sperm preservation. Anim Reprod Sci. 2016;169(1):2-5. http://dx.doi.org/10.1016/j.anireprosci.2016.02.004. PMid:26936658.

Souza DO, Araujo ML, Biscarde CEA, Mendes CS, Silva MAA, Romero DCM, Cardoso BM No, Santana ALA, Barbosa LP. Use of hormonal subdoses applied in Bai Hui acupuncture in estrus synchronization protocols for goats. Semina: Ciênc Agrár. 2019;40(4):1501-12. http://dx.doi.org/10.5433/1679-0359.2019v40n4p1501.

Taberner E, Medrano A, Peña A, Rigau T, Miró J. Oestrus cycle characteristics and prediction of ovulation in Catalonian jennies. Theriogenology. 2008;70(9):1489-97. http://dx.doi.org/10.1016/j.theriogenology.2008.06.096. PMid:18678401.

Tadesse G, Tefera M. Reproductive characteristics of abyssinian jennies exposed to stallions and jackasses. Ethiop Vet J. 2013;17(2):77-84. http://dx.doi.org/10.4314/evj.v17i2.6.

Wang XL, Zhang TF, Zhang HX, Mao HR, Huang GF. Therapeutic effects of acupoint injection at cervical Jiaji points and effects on ET and CGRP in the patient of ischemic stroke. Zhongguo Zhen Jiu. 2007;27(2):93-5. PMid:17370487.

Wilson CG, Downie CR, Hughes JP, Roser JF. Effects of repeated hCG injections on reproductive efficiency in mares. J Equine Vet Sci. 1990;10(4):301-8. http://dx.doi.org/10.1016/S0737-0806(06)80015-8.

Wynn SG, Luna SPL, Liu H, Xie H, Nan TC, Chien CH. Global acupuncture research: previously untranslated studies. Studies from Brazil. In: Schoen AM, editor. Veterinary acupuncture: ancient art to modern medicine. St Louis: Mosby; 2001. p. 53-7.
 


Submitted date:
09/04/2020

Accepted date:
10/27/2020

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