Animal Reproduction (AR)
Animal Reproduction (AR)

Fixed-time artificial insemination protocols on brazilian locally adapted breed gilts on ovulatory response and embryo production

Priscilla Cristine Passoni Silva; Oscar Oliveira Brasil; Paula Lorena Grangeira Souto; Nathalia Hack Moreira; Joseane Padilha da Silva; Bianca Damiani Marques Silva; Alexandre Floriani Ramos

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The aim of this study was to use estrus synchronization protocols to favor fixed-time artificial insemination and consequently fixed-time embryo collection, and increase embryo production using eCG, in gits. In a cross over design, nine Piau breed gilts were subjected to 18 days of oral progesterone; P4 group did not receive any further; GnRH group received 25µg of GnRH 104 hours after the final application of P4; and eCG+GnRH group received 1000IU of eCG 24 hours after the final P4 in addition to GnRH for subsequent embryo collection, that was performed six days after first AI, by laparotomy. Artificial insemination was performed after 12 and 24 hours of estrus in P4 group, and 128 and 144 hours in GnRH and eCG+GnRH groups. The number of CL (8.6±3.9; 8.3±2.1; 26.7±15.0) and anovulatory follicles (4.3±3.7; 3.9±3.9; 17.2±9.5) was higher in the eCG+GnRH gilts (P<0.05). However, the use of 1000 IU of eCG reduced (P<0.05) the number of total structures (5.2±3.6; 5.1±3.1; 1.7±2.7), viable embryos (5.0±3.5; 4.8±3.3; 0.4±0.7), freezable embryos (3.6±3.4; 3.3±3.8; 0.1±0.3) and recovery rate (63.7±38.9; 58.6±24.7; 5.38±9.5). P4 and GnRH protocols were effective in the production and recovery of embryos. However, the use of 1000 IU of eCG, 24 hours after P4, was not effective in promoting the production of embryos, although the animals had superovulated.


synchronization protocol, fixed time artificial insemination, embryo recovery, pig, genetic resources


Barton BE, Herrera GG, Anamthathmakula P, Rock JK, Willie AM, Harris EA, Takemaru KI, Winuthayanon W. Roles of steroid hormones in oviductal function. Reproduction. 2020;159(3):R125-37. PMid:32040278.

Besenfelder U, Mödl J, Müller M, Brem G. Endoscopic embryo collection and embryo transfer into the oviduct and the uterus of pigs. Theriogenology. 1997;47(5):1051-60. PMid:16728055.

Brüssow KP, Schneider F, Kanitz W, Rátky J, Kauffold J, Wähner M. Studies on fixed-time ovulation induction in the pig. Soc Reprod Fertil Suppl. 2009;66:187-95. PMid:19848281.

De Rensis F, Benedetti S, Silva P, Kirkwood RN. Fertility of sows following artificial insemination at a gonadotrophin-induced estrus coincidente with weaning. Anim Reprod Sci. 2003;76(3-4):245-50. PMid:12586496.

De Rensis F, Kirkwood RN. Control of estrus and ovulation: fertility to timed insemination of gilts and sows. Theriogenology. 2016;86(6):1460-6. PMid:27287469.

Driancourt MA, Cox P, Rubion S, Harnois-Milon G, Kemp B, Soede NM. Induction of LH surge and ovulation by buserelin (as Receptal) allows breeding of weaned sows with a single fixed-time insemination. Theriogenology. 2013;80(4):391-9. PMid:23777891.

Fujino Y, Nakamura Y, Kobayashi H, Kikuchi K. Relationship between time elapsed after human chorionic gonadotropin administration and developmental stage in porcine embryos collected from prepubertal gilts. Vol. 52. J Reprod Dev. 2006;52(2):267-75. PMid:16462095.

Grégoire A, Allard A, Huamán E, León S, Silva RM, Buff S, Berard M, Joly T. Control of the estrous cycle in guinea-pig (Cavia porcellus). Theriogenology. 2012;78(4):842-7. PMid:22626773.

Hazeleger W, Bouwman EG, Noordhuizen JPTM, Kemp B. Effect of superovulation induction on embryonic development on day 5 and subsequent development and survival after nonsurgical embryo transfer in pigs. Theriogenology. 2000;53(5):1063-70. PMid:10798484.

He Y, Qu Y, Jing JC, Chen Z. Characterization of oviduct ciliary beat frequency using real time phase resolved Doppler spectrally encoded interferometric microscopy. Biomed Opt Express. 2019;10(11):5650-9. PMid:31799037.

Herrmann HH, Holtz W. Culture of pig embryos collected in situ or after slaughter. Anim Reprod Sci. 1981;4(2):143-7.

Hribal R, Guenther A, Rübensam K, Jewgenow K. Blastocyst recovery and multifactorial gene expression analysis in the wild guinea pig (Cavia aperea). Theriogenology. 2016;86(5):1299-307. PMid:27264741.

Jabbour HN, Evans G. Ovarian and endocrine responses of merino ewes following treatment with PMSG and GnRH or PMSG antiserum. Anim Reprod Sci. 1991;24(3-4):259-70.

Kauffold J, Beckjunker J, Kanora A, Zaremba W. Synchronization of estrus and ovulation in sows not conceiving in a scheduled fixed-time insemination program. Anim Reprod Sci. 2007;97(1-2):84-93. PMid:16481132.

Kirkwood RN, Kauffold J. Advances in breeding management and use of ovulation induction for fixed-time AI. Reprod Domest Anim. 2015;50(Suppl 2):85-9. PMid:26174924.

Koutsotheodoros F, Hughes PE, Parr RA, Dunshea FR, Fry RC, Tilton JE. The effects of post-weaning progestagen treatment (Regumate) of early-weaned primiparous sows on subsequent reproductive performance. Anim Reprod Sci. 1998;52(1):71-9. PMid:9728816.

Kraeling RR, Webel SK. Current strategies for reproductive management of gilts and sows in North America. J Anim Sci Biotechnol. 2015;6(1):3. PMid:25838898.

Li S, Winuthayanon W. Oviduct: roles in fertilization and early embryo development. J Endocrinol. 2017;232(1):R1-26. PMid:27875265.

Mariante AS, Egito AA. Animal genetic resources in brazil: result of five centuries of natural selection. Theriogenology. 2002;57(1):223-35. PMid:11775972.

Martinat-Botté F, Bariteau F, Forgerit Y, Macar C, Moreau A, Terqui M, Signoret JP. Control of oestrus in gilts II. Synchronization of oestrus with a progestagen, altrenogest (Regumate): effect on fertility and litter size. Anim Reprod Sci. 1990;22(3):227-33.

Martinat-Botté F, Bariteau F, Forgerit Y, Macar C, Poirier P, Terqui M. Synchronization of oestrus in gilts with altrenogest: effects on ovulation rate and foetal survival. Anim Reprod Sci. 1995;39(4):267-74.

Martinat-Botté F, Venturi E, Guillouet P, Driancourt MA, Terqui M. Induction and synchronization of ovulations of nulliparous and multiparous sows with an injection of gonadotropin-releasing hormone agonist (Receptal). Theriogenology. 2010;73(3):332-42. PMid:19962182.

Martinez EA, Martinez CA, Cambra JM, Maside C, Lucas X, Vazquez JL, Vazquez JM, Roca J, Rodriguez-Martinez H, Gil MA, Parrilla I, Cuello C. Achievements and future perspectives of embryo transfer technology in pigs. Reprod Domest Anim. 2019;54(Suppl 4):4-13. PMid:31625238.

Montes JC, Penitente-Filho JM, Guimarães SEF, Lopes PS, Camilo BS, Shiomi HH, Lima DA, Pinho RO, Pereira JVTDN, Okano DS, Costa KA, Guimarães JD. Aspects of sexual precocity and morphometry of uterus, placenta and embryos/fetuses in Piau breed and Commercial line gilts. Theriogenology. 2018;105:75-83. PMid:28930668.

Peltoniemi O, Björkman S, Oropeza-Moe M, Oliviero C. Developments of reproductive management and biotechnology in the pig. Anim Reprod. 2019;16(3):524-38. PMid:32435295.

Rátky J, Brüssow KP, Solti L, Torner H, Sarlós P. Ovarian response, embryo recovery and results of embryo transfer in a Hungarian native pig breed. Theriogenology. 2001;56(5):969-78. PMid:11665896.

Schlieper B, Holtz W. Transfer of pig embryos collected by laparotomy or slaughter. Anim Reprod Sci. 1986;12(2):109-14.

Silva PV, Guimarães SEF, Guimarães JD, Nascimento CS, Lopes PS, Siqueira JB, Amorim LS, Fonseca E Silva F, Foxcroft GR. Follicular dynamics and gene expression in granulosa cells, corpora lutea and oocytes from gilts of breeds with low and high ovulation rates. Reprod Fertil Dev. 2014;26(2):316-27. PMid:23464354.

Silva PV, Guimarães SEF, Guimarães JD, Neto JB, Lopes PS, do Nascimento CS, de Campos CF, Weller MM, Botelho ME, Faria VR. Gene expression in swine granulosa cells and ovarian tissue during the estrous cycle. Genet Mol Res. 2011;10(3):2258-67. PMid:21968765.

Soede NM, Langendijk P, Kemp B. Reproductive cycles in pigs. Anim Reprod Sci. 2011;124(3-4):251-8. PMid:21397415.

Sommer JR, Collins EB, Estrada JL, Petters RM. Synchronizacion and superovulation of mature cycling gilts for the collection of pronuclear stage embryos. Anim Reprod Sci. 2007;100(3-4):402-10. PMid:17118586.

Stringfellow DA, Seidel SM. Manual da Sociedade Internacional de Transferência de Embriões. 3rd ed. EUA: IETS; 1998.

Tummaruk P, Roongsitthichai A, Rensis F. Ovulation induction in sows. Wetchasan Sattawaphaet. 2011;41:19-23.

Wang Z, Liu BS, Wang XY, Wei QH, Tian H, Wang LQ. Effects of altrenogest on reproductive performance of gilts and sows: A meta-analysis. Anim Reprod Sci. 2018;197:10-21. PMid:30197055.

Wollenberg C, Wentz I, Blum B, Holtz W. Survival of pig embryos flushed from the reproductive tract immediately or two hours after slaughter of donors. J Anim Sci. 1990;68(7):2023-6. PMid:2384392.

Ziecik AJ, Klos J, Przygrodzka E, Milewski R, Jana B. Aberrant effects of altrenogest and exposure to exogenous gonadotropins on follicular cysts appearance in gilts. Theriogenology. 2017;89:250-4. PMid:28043359.

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