Body measurements correlation and x-ray imaging of three Hystrix javanica fetuses in different fetal development stages

Yosua Kristian Adi; Surya Agus Prihatno; Irma Padeta; Teguh Budipitojo

doi:10.1590/1984-3143-AR2021-0005

ORIGINAL ARTICLE

Body measurements correlation and x-ray imaging of three Hystrix javanica fetuses in different fetal development stages

Yosua Kristian Adi; Surya Agus Prihatno; Irma Padeta; Teguh Budipitojo

http://dx.doi.org/10.1590/1984-3143-AR2021-0005 Anim Reprod, vol.18, n3, e20210005, 2021

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Abstract

Hystrix javanica is endemic species in Indonesia. Study about fetal development of Hystrix javanica are very rare because of sample limitation. This study was carried out to describe the morphometrics and x-ray analysis of three fetuses in different stage to give basic information about fetal development of Hystrix javanica. Three fetus samples fixed in Bouin’s solution was used in this study. Observation was carried out to identify the characteristic of three fetus samples. This included the pattern of hair, body measurements, body volume, and body weight. X-ray analysis was carried out to know the ossification process in the fetal development. Statistical analysis was carried out using Microsoft 365® Excel program software. Three fetus samples had different specific hair pattern, that was hairless, smooth hairs, and smooth hairs with dense-non dense pattern. Body volume of 1^st, 2^nd, and 3^rd fetus were 23mL, 90mL, and 170mL, respectively. Body weight of 1^st, 2^nd, and 3^rd fetus were 19.5g, 79.22g, and 153.18g, respectively. Pearson’s correlation analysis shown strong relationship between total body length, front body length, back body length, horizontal body diameter, vertical body diameter, head length, and head diameter against body volume and body weight of three fetuses. Significant positive correlation was shown between horizontal body diameter, vertical body diameter, and head diameter against body volume and body length with P value < 0.05. Faint radiopaque images showed in the 2^nd fetus sample and strong radiopaque images showed in the 3^rd fetus sample. Radiopaque images were identified in the teeth, cranium, vertebrae, and extremities bones. In this study we concluded that there was a specific hair pattern in different fetal stage. All body measurements have positive correlation with body volume and body weight and x-ray analysis shown that the ossification of the bone was started to happen while the smooth hair was growth.

Keywords

linear regression, measurements, morphology, radiography, sunda porcupine fetus

References

Aplin K. Hystrix javanica. Cambridge: The IUCN Red List of Threatened Species; 2016. e.T10752A22231749. https://dx.doi.org/10.2305/IUCN.UK.2016-2.RLTS.T10752A22231749.en.

Beccaglia M, Alonge S, Trovo’ C, Luvoni GC. Determination of gestational time and prediction of parturition in dogs and cats: an update. Reprod Domest Anim. 2016;51(1, Suppl 1):12-7. http://dx.doi.org/10.1111/rda.12782. PMid:27670935.

Begall S, Burda H, Gallardo MH. Reproduction, postnatal development, and growth of cocial coruros, Spalacopus cyanus (Rodentia: octodontidae), from Chile. J Mammal. 1999;80(1):210-7. http://dx.doi.org/10.2307/1383221.

Behroozian M, Ejtehadi H, Memariani F, Pierce S, Mesdaghi M. Are endemic species necessarily ecological specialists? Functional variability and niche differentiation of two threatened Dianthus species in the montane steppes of northeastern Iran. Sci Rep. 2020;10(1):11774. http://dx.doi.org/10.1038/s41598-020-68618-7. PMid:32678159.

Bisaz R, Sullivan RM. Developmental neurobiology of the rat attachment system and its modulation by stress. Behav Sci (Basel). 2012;2(2):79-102. http://dx.doi.org/10.3390/bs2020079. PMid:23626910.

Burda H. Relationships among rodent taxa as indicated by reproductive biology1. J Zool Syst Evol Res. 1989;27(1):49-57. http://dx.doi.org/10.1111/j.1439-0469.1989.tb00438.x.

Chung Y, Lim N, Shunari M, Wang D, Chan S. Records of the Malayan porcupine, Hystrix brachyura (Mammalia: Rodentia: Hystricidae) in Singapore. Nat Singap. [serial on the Internet]. 2016 [cited 2021 Jan 25];9:63-8. Available from: https://lkcnhm.nus.edu.sg/wp-content/uploads/sites/10/app/uploads/2017/06/2016nis063-068.pdf

Derrickson EM. Comparative reproductive strategies of altricial and precocial eutherian mammals. Funct Ecol. 1992;6(1):57-65. http://dx.doi.org/10.2307/2389771.

El Bizri HR, Monteiro FOB, de Andrade RDS, Valsecchi J, Guimarães DAA, Mayor P. Embryonic and fetal morphology in the lowland paca (Cuniculus paca): A precocial hystricomorph rodent. Theriogenology. 2017;104:7-17. http://dx.doi.org/10.1016/j.theriogenology.2017.08.004. PMid:28802114.

Evans HE, Sack WO. Prenatal development of domestic and laboratory mammals: growth curves, external features and selected references. Zentralbl Veterinärmed C. 1973;2(1):11-45. http://dx.doi.org/10.1111/j.1439-0264.1973.tb00253.x. PMid:4745140.

Felipe AE, Masson PG. Observations on the Fetal Morphology in Myocastor Coypus Bonariensis (Coypu) (Rodentia, Myocastoridae). Anat Histol Embryol. 2008;37(6):469-74. http://dx.doi.org/10.1111/j.1439-0264.2008.00884.x. PMid:19032632.

Fortes EA, Ferraz MS, Bezerra DO, Conde AM Jr, Cabral RM, Sousa F, Almeida HM, Pessoa GT, Menezes DJ, Guerra SP, Sampaio IB, Assis AC No, Carvalho MA. Prenatal development of the agouti (Dasyprocta prymnolopha Wagler, 1831): external features and growth curves. Anim Reprod Sci. 2013;140(3-4):195-205. http://dx.doi.org/10.1016/j.anireprosci.2013.06.021. PMid:23891216.

Garbis-Berkvens JM, Peters PWJ. Development of the skeleton, rat. In: Jones TC, Mohr U, Hunt RD, editors. Cardiovascular and musculoskeletal systems. monographs on pathology of laboratory animals (sponsored by the International Life Sciences Institute). Berlin, Heidelberg: Springer; 1991. http://dx.doi.org/10.1007/978-3-642-76533-9_27

Hall RJ, Milner‐Gulland EJ, Courchamp F. Endangering the endangered: the effects of perceived rarity on species exploitation. Conserv Lett. 2008;1(2):75-81. http://dx.doi.org/10.1111/j.1755-263X.2008.00013.x.

Inayah N, Sari AP, Farida WR, Nugroho HA, Handayani TH, Amalia RLR, Shidiq F. Diet enrichment and the reproductive season of captive Sunda Porcupine (Hystrix javanica F. Cuvier 1823). BIO Web of Conf. 2020;19:00011. https://doi.org/10.1051/bioconf/20201900011.

Kaufman MH. The atlas of mouse development. San Diego: Academic; 1992.

Limmanont C, Ponglowhapan S, Tanhan P, Sirinarumitr T, Sirinarumitr K. Fetal head diameter in dogs and cats measured by radiography and ultrasonography. TJVM. 2019;49(2):175-82. https://he01.tci-thaijo.org/index.php/tjvm/article/view/223909.

Luvoni GC, Grioni A. Determination of gestational age in medium and small size bitches using ultrasonographic fetal measurements. J Small Anim Pract. 2000;41(7):292-4. http://dx.doi.org/10.1111/j.1748-5827.2000.tb03204.x. PMid:10976623.

Mao WH, Albrecht E, Teuscher F, Yang Q, Zhao RQ, Wegner J. Growth- and breed-related changes of fetal development in cattle. Asian-Aust J Anim Sci. [serial on the Internet]. 2008 [cited 2021 Jan 25];21(5):640-7. Available from: https://www.animbiosci.org/upload/pdf/21-90.pdf

Mayor P, El Bizri H, Bodmer RE, Bowler M. Assessment of mammal reproduction for hunting sustainability through community-based sampling of species in the wild. Conserv Biol. 2017;31(4):912-23. http://dx.doi.org/10.1111/cobi.12870. PMid:27917537.

Mustikasari IA, Withaningsih S, Megantara EN, Husodo T, Parikesit P. Population and distribution of Sunda porcupine (Hystrix javanica F. Cuvier, 1823) in designated area of Cisokan Hydropower, West Java, Indonesia. Biodiversitas (Surak). 2019;20(3):762-9. http://dx.doi.org/10.13057/biodiv/d200321.

Nowak R. Walker’s mammals of the world. 6th ed. Baltimore: Johns Hopkins University Press; 1964, Vol. 2, Old World porcupines.

Oliveira GB, Araújo HN Jr, Costa HS, Silva AR, Moura CEB, Rocha HAO, Miglino MA, Oliveira MF. Post-implantation development of red-rumped agouti (Dasyprocta leporina Linnaeus, 1758). Anim Reprod Sci. 2017;182:35-47. http://dx.doi.org/10.1016/j.anireprosci.2017.04.007. PMid:28502649.

Theiler K. The house mouse: atlas of embryonic development. New York: Springer-Verlag; 1989. http://dx.doi.org/10.1007/978-3-642-88418-4.

van Zalen-Sprock RM, Brons JT, van Vugt JM, van der Harten HJ, van Geijn HP. Ultrasonographic and radiologic visualization of the developing embryonic skeleton. Ultrasound Obstet Gynecol. 1997;9(6):392-7. http://dx.doi.org/10.1046/j.1469-0705.1997.09060392.x. PMid:9239824.

Witschi E. Rat development. In: Altman PL, Dittmer DS. editors. Growth including reproduction and morphological development. Washington: Federation of American Societies for Experimental Biology; 1962. p. 304-14.

Yilmaz S, Tokpınar A. Double staining in rats (mini review). Biomed J Sci & Tech Res. 2019;16(4):12282-5. http://dx.doi.org/10.26717/BJSTR.2019.16.002899.

Submitted date:
01/15/2021

Accepted date:
07/19/2021

Body measurements correlation and x-ray imaging of three Hystrix javanica fetuses in different fetal development stages

Yosua Kristian Adi; Surya Agus Prihatno; Irma Padeta; Teguh Budipitojo

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