Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.1590/1984-3143-AR2021-0013
Animal Reproduction (AR)
ORIGINAL ARTICLE

Haematological changes in rabbits (Oryctolagus cuniculus f. domesticus) in the course of pregnancy

Maria Chmurska-Gąsowska; Bartosz Bojarski; Leszek Szała

Downloads: 2
Views: 30

Abstract

The analysis of haematological parameters is an important element of the assessment of the physiological condition of animals. Haematological parameters may change both under the influence of various external factors, and in the course of normal pregnancy, which has been found in various species of mammals, including rabbits. Our study showed statistically significant (p<0.05) changes in basic haematological parameters: RBC (decrease; 5.87±0.48 at day 15 vs. 5.42±0.32 T/L at day 26), MCH (increase; 1.35±0.04 before matching vs. 1.41±0.03 fmol at day 26), RDW (decrease; 15.77±1.80 at day 15 vs. 14.27±1.57% at day 26) MPV (increase; 5.17±0.31 at day 15 vs. 5.92±0.70 fL at day 26), WBC (decrease; 8.60±2.57 at day 15 vs. 4.94±0.88 G/L at day 26) and PLT (decrease; 398.17±91.67 before matching vs. 271.67±61.72 G/L at day 26) in Termond White rabbits and RBC (decrease; 6.18±0.68 before matching vs. 5.68±0.54 T/L at day 26), Hb (decrease; 8.00±0.90 before matching vs. 7.32±0.71 mmol/L at day 26), MCH (decrease; 1.32±0.05 at day 15 vs. 1.29±0.04 fmol at day 26) and WBC (decrease; 9.62±1.81 before matching vs. 5.85±2.23 G/L at day 26 as well as 9.58±2.35 at day 15 vs. 5.85±2.23 G/L at day 26) in Popielno White rabbits. Moreover, in the Popielno White rabbits we recorded a significant (p<0.05) decrease in the percentage of irregular erythrocytes at the end of pregnancy (11.00±10.02 at day 15 vs. 3.00±4.94 at day 26). The changes appear to be physiological but should be considered in studies using rabbits as model organisms.

Keywords

rabbits, physiology, haematology, erythrogram, pregnancy

References

Al-Eissa MS. Effect of gestation and season on the haematological and biochemical parameters in domestic rabbit (Oryctolagus cuniculus). Br Biotechnol J. 2011;1(1):10-7. http://dx.doi.org/10.9734/BBJ/2011/226.

Barron WM. Volume homeostasis during pregnancy in the rat. Am J Kidney Dis. 1987;9(4):296-302. http://dx.doi.org/10.1016/S0272-6386(87)80125-7. PMid:3555001.

Bernstein IM, Ziegler W, Badger GJ. Plasma volume expansion in early pregnancy. J Obstet Gynaecol. 2001;97(5 Pt 1):669-72. PMid:11339913.

Bojarski B, Lutnicka H, Swadiba-Karbowy M, Makulska J, Jakubiak M, Pawlak K, Tombarkiewicz B, Witeska M. Effects of herbicides pendimethalin and ethofumesate on common carp (Cyprinus carpio) erythrocyte morphology. Folia Biol. 2018;66(3):143-9. http://dx.doi.org/10.3409/fb_66-3.15.

Bonelli F, Rota A, Corazza D, Serio D, Sgorbini M. Hematological and biochemical findings in pregnant, postfoaling, and lactating jennies. Theriogenol. 2016;85(7):1233-8. http://dx.doi.org/10.1016/j.theriogenology.2015.12.004. PMid:26792379.

Burgos-Aceves MA, Lionetti L, Faggio C. Multidisciplinary haematology as prognostic device in environmental and xenobiotic stress-induced response in fish. Sci Total Environ. 2019;670:1170-83. http://dx.doi.org/10.1016/j.scitotenv.2019.03.275. PMid:31018433.

Bussabarger RA, Cuthbert FP, Ivy AC. Studies on the anemia of pregnancy in gastrectomized and normal dogs. J Lab Clin Med. 1938;24:24-33.

Cetin N, Bekyurek T, Cetin E. Effects of sex, pregnancy and season on some haematological and biochemical blood values in Angora rabbits. Scand J Lab Anim Sci. 2009;36:155-62.

Christopher MM, Hawkins MG, Burton AG. Poikilocytosis in rabbits; prevalence, type, and association with disease. PLoS One. 2014;9(11):e112355. http://dx.doi.org/10.1371/journal.pone.0112455. PMid:25402479.

De Rijk EP, van Esch E, Flik G. Pregnancy dating in the rat: placental morphology and maternal blood parameters. Toxicol Pathol. 2002;30(2):271-82. http://dx.doi.org/10.1080/019262302753559614. PMid:11950171.

Dimco E, Abeshi J, Lika E, Dhamo G. Effect of pregnancy in hematological profile in dogs. Alb J Agric Sci. 2013;12:159-62.

Fay RA, Hughes AO, Farron NT. Platelets in pregnancy: hyperdestruction in pregnancy. Obstet Gynecol. 1983;61(2):238-40. PMid:6823364.

Haneda R, Mizoguchi Y, Matsuoka T, Mizuguchi H, Endoh T, Fukuda K, Asano Y. Changes in blood parameters in pregnant Japanese White rabbits. J Toxicol Sci. 2010;35(5):773-8. http://dx.doi.org/10.2131/jts.35.773. PMid:20930472.

Hart MV, Hosenpud JD, Hohimer AR, Morton MJ. Hemodynamics during pregnancy and sex steroid administration in guinea pigs. Am J Physiol Regul Integr Comp Physiol. 1985;249(2 Pt 2):179-85. http://dx.doi.org/10.1152/ajpregu.1985.249.2.R179. PMid:4025575.

Honda T, Honda K, Kokubun C, Nishimura T, Hasegawa M, Nishida A, Inui T, Kitamura K. Time-course changes of hematology and clinical chemistry values in pregnant rats. J Toxicol Sci. 2008;33(3):375-80. http://dx.doi.org/10.2131/jts.33.375. PMid:18670170.

Horowitz KM, Ingardia CJ, Borgida AF. Anemia in pregnancy. Clin Lab Med. 2013;33(2):281-91. http://dx.doi.org/10.1016/j.cll.2013.03.016. PMid:23702118.

Kim JC, Yun HI, Cha SW, Kim KH, Koh WS, Chung MK. Haematological changes during normal pregnancy in New Zealand White rabbits: a longitudinal study. Comp Clin Pathol. 2002;11(2):98-106. http://dx.doi.org/10.1007/s005800200006.

Kritsepi-Konstantinou K, Oikonomidis IL. The interpretation of erythrogram in dog and cat. Hellen J Compan Anim Med. 2016;5:1-16.

Miresan V, Radacu C, Odagiru A, Longodor A, Balata I, Coroian A. Influence of pregnancy semester on hematolgical and biochemical profile in women. Pro Environ. 2017;10:282-5.

Mizoguchi Y, Matsuoka T, Mizuguchi H, Endoh T, Kamata R, Fukuda K, Ishikawa T, Asano Y. Changes in blood parameters in New Zealand White rabbits during pregnancy. Lab Anim. 2010;44(1):33-9. http://dx.doi.org/10.1258/la.2009.008002. PMid:19854754.

Mostello D, Chalk C, Khoury J, Mack CE, Siddiqi TA, Clark KE. Chronic anemia in pregnant ewes: maternal and fetal effects. Am J Physiol Regul Integr Comp Physiol. 1991;261(5 Pt 2):1075-83. http://dx.doi.org/10.1152/ajpregu.1991.261.5.R1075. PMid:1951756.

Nuwayhid B. Hemodynamic changes during pregnancy in the rabbit. Am J Obstet Gynecol. 1979;135(5):590-6. http://dx.doi.org/10.1016/S0002-9378(16)32982-9. PMid:507110.

Ozegbe PC. Influence of pregnancy on some erythrocyte biochemical profiles in the rabbits. Afr J Biomed Res. 2001;4:135-7.

Pagano M, Faggio C. The use of erythrocyte fragility to assess xenobiotic cytotoxicity. Cell Biochem Funct. 2015;33(6):351-5. http://dx.doi.org/10.1002/cbf.3135. PMid:26399850.

Papworth TA, Clubb SK. Clinical pathology in the female rat during the pre- and postnatal period. Comp Haematol Int. 1995;5(1):13-24. http://dx.doi.org/10.1007/BF00214486.

Poljicak-Milas N, Kardum-Skelin I, Vudan M, Marenjak TS, Ballarin-Perharic A, Milas Z. Blood cell count analyses and erythrocyte morphometry in New Zealand white rabbits. Vet Arh. 2009b;79:561-71.

Poljicak-Milas N, Marenjak TS, Slavica A, Janicki Z, Filipovic N, Sruk V. Comparative hematological and biochemical values in pregnant and non-pregnant red, Cervus elaphus, and fallow deer, Dama dama, females. Folia Zool (Brno). 2009a;58:36-44.

Roy S, Roy M, Mishra S. Hematological and biochemical profile during gestation period in Sahiwal cows. Vet World. 2010;3:26-8.

Silva-Herdade AS, Andolina G, Faggio C, Calado A, Saldanha C. Erythrocyte deformability: A partner of the inflammatory response. Microvasc Res. 2016;107:34-8. http://dx.doi.org/10.1016/j.mvr.2016.04.011. PMid:27142964.

Simsek O, Arikan S, Cinar M. Reference values for selected hematological and biochemical blood parameters from pregnancy to advanced gestation in Angora cats. Turk J Vet Anim Sci. 2015;39:29-33. http://dx.doi.org/10.3906/vet-1405-2.

Soma-Pillay P, Nelson-Piercy C, Tolppanen H, Mebazaa A. Physiological changes in pregnancy. Cardiovasc Afr. 2016;27(2):89-94. http://dx.doi.org/10.5830/CVJA-2016-021. PMid:27213856.

Sukar KAO, Abdelatif AM, Alfaki EM. Effect of pregnancy on thermoregulation, blood constituents, serum bio-chemicals and cortisol level in (Oryctolagus cuniculus) rabbit model. Asian J Biol. 2020;9:44-61. http://dx.doi.org/10.9734/ajob/2020/v9i130077.

Sula E, Aliko V, Pagano M, Faggio C. Digital light microscopy as a tool in toxicological evaluation of fish erythrocyte morphological abnormalities. Microsc Res Tech. 2020;83(4):362-9. http://dx.doi.org/10.1002/jemt.23422. PMid:31825143.

Waziri MA, Ribadu AY, Sivachelvan N. Changes in the serum proteins, hematological and some serum biochemical profiles in the gestation period in the Sahelgoats. Vet Arh. 2010;80:215-24.

Wells MY, Decobecq CPM, Decouvelaere DM, Justice C, Guittin P. Changes in clinical pathology parameters during gestation in the New Zeland White Rabbit. Toxicol Pathol. 1999;27(3):370-9. http://dx.doi.org/10.1177/019262339902700315. PMid:10356716.

Witeska M, Kondera E, Szczygielska K. The effects of cadmium on common carp erythrocyte morphology. Pol J Environ Stud. 2011;20:783-8.
 


Submitted date:
02/26/2021

Accepted date:
06/07/2021

60e6ecbea9539502eb4d62a4 animreprod Articles
Links & Downloads

Anim Reprod

Share this page
Page Sections