Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.1590/1984-3143-AR2021-0015
Animal Reproduction (AR)
ORIGINAL ARTICLE

Spermatozoon head size – the main differentiating feature between spermatozoa of blue and white Arctic fox (Vulpes lagopus)

Karolina Stasiak; Dorota Cygan-Szczegielniak; Joanna Bogucka

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Abstract

Morphology and sperm morphometry, this is an important determinant of male reproductive capacity. Morphometric data may provide relevant information in studies focused on evolutionary biology, sperm quality assessment, including prediction of the potential fertility, semen cryopreservation, or the effect of reprotoxicants. The paper presents the morphometric analysis of spermatozoa from two colour morphs of Arctic fox (Vulpes lagopus), and attempts to determine the relationship between selected quality indicators and dimensions and shape of spermatozoa. The research material consisted of ejaculates collected once by manual stimulation from 20 one-year-old Arctic foxes (10 individuals of the blue morph and 10 of the white morph). Ejaculates were analysed for standard parameters (volume, sperm concentration, total number of spermatozoa in the ejaculate) and used for the preparation of microscopic specimens. It was found that, the dimensions of spermatozoa from Arctic foxes depend on the male colour morphs. Spermatozoa from white Arctic foxes were significantly longer (by 1.82 µm) and had larger heads (0.32 µm longer and 0.15 µm wider) compared to spermatozoa from blue Arctic foxes (P<0.05). The interactions between particular sperm dimensions indicated the occurrence of gametes differing in shape. The all correlation coefficients between the morphometric traits of spermatozoa were statistically significant. Our research proved that in the blue Arctic foxes, sperm dimensions (tail length and total sperm length) can be related to the percentage of spermatozoa with primary changes (respectively: r = -0.68 and r = -0.75; at P <0.05). However, in the case of white Arctic foxes, these characteristics depend on the ejaculate volume (respectively: r = 0.65 and r = 0.68; at P <0.05).

Keywords

arctic fox, ejaculate, sperm dimensions, sperm morphometry

References

Andraszek K., Szeleszczuk O., Niedbała P. Kuchta-Gładysz M. Preliminary research on evaluation of sperm morphometry and chromatin structure in the semen of silver fox (Vulpes vulpes). Folia Pomer Univ Technol Stetin Agric Aliment Pisc Zootech. 2016, 326(38)2, 5-16. http://dx.doi.org/10.21005/AAPZ2016.38.2.01.

Antończyk A. Computer analysis of the motility and morphology of dog sperm in fresh and cryopreserved semen [thesis]. Wroclaw: Wroclaw University of Environmental and Life Sciences; 2012. Polish.

Banaszewska D, Kondracki S, Wysokińska A. Effect of sperm concentration on ejaculate for morphometric traits of spermatozoas of the Pietrain breed boars. J Cent Eur Agric. [serial on the Internet]. 2009 [cited 2021 Feb 25];10(4):383-95. Available from: https://jcea.agr.hr/en/issues/article/747

Banaszewska D, Kondracki S. An assessment of the breeding maturity of insemination boars based on ejaculate quality changes. Folia Biol (Krakow). 2012;60(3-4):151-62. http://dx.doi.org/10.3409/fb60_34.151162. PMid:23342910.

Banaszewska D, Andraszek K, Zdrowowicz E, Czubaszek M, Walczak-Jędrzejowska R. The role of staining techniques in seminological analysis of mammalian semen. Folia Pomer Univ Technol Stetin Agric Aliment Pisc Zootech. [serial on the Internet]. 2015a, [cited 2021 Feb 25];320(35)3:5-20. Available from: https://core.ac.uk/download/pdf/160236977.pdf

Banaszewska D, Andraszek K, Szostek M, Wójcik E, Danielewicz A, Walczak-Jędrzejowska R. Analysis of stallion semiologic semen parameters. Med Weter. [serial on the Internet]. 2015b [cited 2021 Feb 25];71(9):563-7. Available from: http://www.medycynawet.edu.pl/images/stories/pdf/pdf2015/092015/201509563567.pdf

Buendia P, Soler C, Paolicchi F, Gago G, Urquieta B, Perez-Sanchez F, Bustos-Obregon E. Morphometric characterization and classification of alpaca sperm heads using the Sperm-Class Analyzer computer-assisted system. Theriogenology. 2002;57(4):1207-18. http://dx.doi.org/10.1016/S0093-691X(01)00724-5. PMid:12013442.

Casey PJ, Gravance CG, Davis RO, Chabot DD, Liu IK. Morphometric differences in sperm head dimensions of fertile and subfertile stallions. Theriogenology. 1997;47(2):575-82. http://dx.doi.org/10.1016/S0093-691X(97)00015-0. PMid:16728009.

Czubaszek M, Andraszek K, Banaszewska D, Walczak-Jędrzejowska R. The effect of the staining technique on morphological and morphometric parameters of boar sperm. PLoS One. 2019;14(3):e0214243. http://dx.doi.org/10.1371/journal.pone.0214243. PMid:30908553.

European Convention. Standing committee of the European Convention for the protection of animals kept for farm-ing purposes. Recommendation concerning fur animals. T‐AP(96)19. Europe: European Convention; 1998.

European Union. Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes. Official Journal of the European Union [Internet]. Bruxelas; 2010 [cited 2021 Feb 25]. Available from: https://eur-lex.europa.eu/legal-content/EN/TXT/PDF/?uri=CELEX:32010L0063&from=EN

Fitzpatrick JL, Lüpold S. Sexual selection and the evolution of sperm quality. Mol Hum Reprod. 2014;20(12):1180-9. http://dx.doi.org/10.1093/molehr/gau067. PMid:25323970.

Gomendio M, Roldan ER. Implications of diversity in sperm size and function for sperm competition and fertility. Int J Dev Biol. 2008;52(5-6):439-47. http://dx.doi.org/10.1387/ijdb.082595mg. PMid:18649256.

Gomendio M, Roldan ER. Sperm competition influences sperm size in mammals. Proc Biol Sci. 1991;243(1308):181-5. http://dx.doi.org/10.1098/rspb.1991.0029. PMid:1675796.

Helfenstein F, Podevin M, Richner H. Sperm morphology, swimming velocity, and longevity in the house sparrow Passer domesticus. Behav Ecol Sociobiol. 2010;64(4):557-65. http://dx.doi.org/10.1007/s00265-009-0871-x.

Hingst O, Blottner S, Franz C. Chromatin condensation in cat spermatozoa during epididymal transit as studied by aniline blue and acridine orange staining. Andrologia. 1995;27(5):275-9. http://dx.doi.org/10.1111/j.1439-0272.1995.tb01105.x. PMid:8659706.

Hirai M, Boersma A, Hoeflich A, Wolf E, Föll J, Auműller R, Braun J. Objectively measured sperm motility and sperm head morphometry in boars (Sus Scrofa): relation to fertility and seminal plasma growth factors. J Androl. 2001;22(1):104-10. http://dx.doi.org/10.1002/j.1939-4640.2001.tb02159.x. PMid:11191073.

Katz DF, Overstreet JW, Samuels SJ, Niswander PW, Bloom TD, Lewis EL. Morphometric analysis of spermatozoa in the assessment of human male fertility. J Androl. 1986;7(4):203-10. http://dx.doi.org/10.1002/j.1939-4640.1986.tb00913.x. PMid:2427496.

Kondracki S, Banaszewska D, Wysokińska A, Sadowska A. Ejaculate traits and spermatozoa morphology as related to spermatozoa concentration in ejaculates of Polish Large White boars. Anim Sci Pap Rep. [cited 2021 Feb 25];2006 [serial on the Internet]; 24(3):111-9. Available from: https://www.researchgate.net/profile/Stanislaw_Kondracki/publication/281412577_Ejaculate_traits_and_spermatozoa_morphology_as_related_to_spermatozoa_concentration_in_ejaculates_of_Polish_Large_White_boars/links/57f3c2ed08ae280dd0b730c2/Ejaculate-traits-and-spermatozoa-morphology-as-related-to-spermatozoa-concentration-in-ejaculates-of-Polish-Large-White-boars.pdf

Kondracki S, Banaszewska D, Wysokńjska A, Iwanina M. The effect of sperm concentration in the ejaculate on morphological traits of bull spermatozoa. Folia Biol (Krakow). 2012;60(1-2):85-91. http://dx.doi.org/10.3409/fb60_1-2.85-91. PMid:22428313.

Kondracki S, Bonaszewska D, Mielnicka C. The effect of age on the morphometric sperm traits of domestic pigs (Sus scrofa domestica). Cell Mol Biol Lett. 2005;10(1):3-13. PMid:15809675.

Kutzler MA. Semen collection in the dog. Theriogenology. 2005;64(3):747-54. http://dx.doi.org/10.1016/j.theriogenology.2005.05.023. PMid:15993482.

Malo AF, Gomendio M, Garde J, Lang-Lenton B, Soler AJ, Roldan ER. Sperm design and sperm function. Biol Lett. 2006;2(2):246-9. http://dx.doi.org/10.1098/rsbl.2006.0449. PMid:17148374.

Maree L, Du Plessis S, Menkveld R, Van der Horst G. Morphometric dimensions of the human sperm head depend on the staining method used. Hum Reprod. 2010;25(6):1369-82. http://dx.doi.org/10.1093/humrep/deq075. PMid:20400771.

Morrow E, Gage M. Consistent significant variation between individual males in spermatozoa morphometry. J Zool (Lond). 2001;254(2):147-53. http://dx.doi.org/10.1017/S0952836901000656.

Nisztuk-Pacek S. Genetic variability of farmed fur animals of the Canidae family assessed by nuclear and mitochondrial DNA analysis. Med. Weter. 2016,72(8):505-10. http://dx.doi.org/10.21521/mw.5544.

Oettlé EE. Sperm morphology and fertility in the dog. J Reprod Fertil Suppl. 1993;47:257-60. PMid:8229933.

Peña FJ, Saravia F, García-Herreros M, Núñez-Martínez I, Tapia JA, Johannisson A, Wallgren M, Rodríguez-Martínez H. Identification of sperm morphometric subpopulations in two different portions of the boar ejaculate and its relation to post thaw quality. J Androl. 2005;26(6):716-23. http://dx.doi.org/10.2164/jandrol.05030. PMid:16291966.

Prisant N, Escalier D, Soufir J, Morillon M, Schoevaert D, Misrahi M, Tachdjian G. Ultrastructural nuclear defects and increased chromosome aneuploidies in spermatozoa with elongated heads. Hum Reprod. 2007;22(4):1052-9. http://dx.doi.org/10.1093/humrep/del481. PMid:17208942.

Rijsselaere T, Van Soom A, Hoflack G, Maes D, de Kruif A. Automated sperm morphometry and morphology analysis of canine semen by the Hamilton-Thorne analyser. Theriogenology. 2004;62(7):1292-306. http://dx.doi.org/10.1016/j.theriogenology.2004.01.005. PMid:15325556.

Smital J. Effects influencing boar semen. Anim Reprod Sci. 2009;110(3-4):335-46. http://dx.doi.org/10.1016/j.anireprosci.2008.01.024. PMid:18367351.

Soler C, Contell J, Bori L, Sancho M, García-Molina A, Valverde A, Segarvall J. Sperm kinematic, head morphometric and kineticmorphometric subpopulations in the blue fox (Alopex lagopus). Asian J Androl. 2017;19(2):154-9. http://dx.doi.org/10.4103/1008-682X.188445. PMid:27751987.

Stasiak K, Kondracki S, Iwanina M. Assessment of chosen semen characteristics of the two colour morphs of the arctic fox (Alopex lagopus L). Anim Sci J. 2019;90(9):1120-6. http://dx.doi.org/10.1111/asj.13257. PMid:31310048.

Tourmente M, Gomendio M, Roldan ER. Sperm competition and the evolution of sperm design in mammals. BMC Evol Biol. 2011;11:12. http://dx.doi.org/10.1186/1471-2148-11-12.

Waheed M, Ghoneim I, Abdou M. Morphometric characteristics of spermatozoa in the Arabian Horse with regard to season, age, sperm concentration, and fertility. J Equine Vet Sci. 2015;35(3):244-9. http://dx.doi.org/10.1016/j.jevs.2015.01.005.

Watson PF. The causes of reduced fertility with cryopreserved semen. Anim Reprod Sci. 2000;60-61:481-92. http://dx.doi.org/10.1016/S0378-4320(00)00099-3. PMid:10844218.

Yániz JL, Soler C, Santolaria P. Computer assisted sperm morphometry in mammals: a review. Anim Reprod Sci. 2015;156:1-12. http://dx.doi.org/10.1016/j.anireprosci.2015.03.002. PMid:25802026.
 


Submitted date:
02/26/2021

Accepted date:
09/16/2021

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