Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.1590/1984-3143-AR2021-0017
Animal Reproduction (AR)
ORIGINAL ARTICLE

Can detomidine replace medetomidine for pharmacological semen collection in domestic cats?

Maitê Cardoso Coelho da Silva; Karitha Marques Ullony; Gediendson Ribeiro de Araújo; Pedro Nacib Jorge-Neto; Verônica Batista Albuquerque; Simone Marques Caramalac; Alice Rodrigues de Oliveira; Ricardo Zanella; Mariana Groke Marques; Antonio Carlos Csemark Junior; Thiago Cavalheri Luczinski; Fabrício de Oliveira Frazílio; Eliane Vianna da Costa e Silva; Thyara de Deco-Souza

Downloads: 0
Views: 668

Abstract

Among the different methods used for semen collection from domestic cats, the pharmacological collection by urethral catheterization becomes disruptive. Medetomidine is the elected α2-adrenoceptor agonist for that, but in several countries, it is not commercially available. This study aimed to evaluate the efficacy of detomidine compared to medetomidine in collecting semen by urethral catheterization in domestic cats. Urethral catheterization was performed on 13 mongrel cats using a disposable semi-rigid tomcat urinary catheter. Of the 19 semen collections performed with medetomidine induction, 94.7% were successful, while with detomidine induction, only 56.3% of 16 were successful. The values semen samples variables were as follows for volume - 10.56 ± 0.4 vs 8.88 ± 0.5 mL, motility - 171.67 ± 0.79 vs 49.77 ± 3.45%, vigor – 4.1 ± 0.03 vs 3.10 ± 0.1 and concentration - 3.24 ± 0.19 vs 2.15 ± 0.13 ×109 sperm/mL respectively for medetomidine and detomidine group. The failure in semen collections with detomidine was mainly due to azoospermic samples, poor urethral relaxation, insufficient volume, or contamination of urine. The sperm concentration was also lower in the detomidine group (P <0.05) when compared to medetomidine. However, when the volume of semen collected was compared, we found no statistical differences. Despite its low performance in collecting semen from cats, detomidine may be an alternative when medetomidine is not accessible.

Keywords

urethral catheterization, α2-adrenoceptor agonist, felids, tomcats

References

Albuquerque VB, Araújo MA, Oliveira AR, Cavalcanti G, Leal RC, Santos ÉB, Cavalcanti AR, Frazilio FO. Evaluation of cardiopulmonary parameters and recovery from anesthesia in cougars (Puma concolor) anesthetized with detomidine/ketamine and isoflurane or sevoflurane. Wildlife Medicine. 2016;36(1):33-8. http://dx.doi.org/10.1590/S0100-736X2016000100006.

Araujo GR, Deco-Souza T, Bergo LCF, Silva LC, Morato RG, Jorge-Neto PN, Silva MCC, Macedo GG, Paula TAR. Field friendly method for wild feline semen cryopreservation. J Threat Taxa. 2020a;12(5):15557-64. http://dx.doi.org/10.11609/jott.5744.12.5.15557-15564.

Araujo GR, Paula TAR, Deco-Souza T, Morato RG, Bergo LCF, Silva LCD, Costa DS, Braud C. Comparison of semen samples collected from wild and captive jaguars (Panthera onca) by urethral catheterization after pharmacological induction. Anim Reprod Sci. 2018;195:1-7. http://dx.doi.org/10.1016/j.anireprosci.2017.12.019. PMid:29935916.

Araujo GR, Paula TAR, Deco-Souza T, Morato RG, Bergo LCF, Silva LC, Jorge-Neto PN, Sampaio BFB. Colheita farmacológica de sêmen de onças-pardas (Puma concolor: Mammalia: Carnivora: Felidae). Arq Bras Med Vet Zootec. 2020b;72(2):437-42. http://dx.doi.org/10.1590/1678-4162-11030.

Axnér E. Sperm maturation in the domestic cat. Theriogenology. 2006;66(1):14-24. http://dx.doi.org/10.1016/j.theriogenology.2006.03.022. PMid:16620928.

Cunto M, Küster D, Bini C, Cartolano C, Pietra M, Zambelli D. Influence of Different Protocols of Urethral Catheterization after Pharmacological Induction (Ur.Ca.P.I.) on Semen Quality in the Domestic Cat. Reprod Domest Anim. 2015;50(6):999-1002. http://dx.doi.org/10.1111/rda.12626. PMid:26482317.

Dobson H, Smith RF. What is stress, and how does it affect reproduction? Anim Reprod Sci. 2000;60–61:743-52. http://dx.doi.org/10.1016/S0378-4320(00)00080-4. PMid:10844239.

Dobson H, Tebble JE, Smith RF, Ward WR. Is stress really all that important? Theriogenology. 2001;55(1):65-73. http://dx.doi.org/10.1016/S0093-691X(00)00446-5. PMid:11198089.

Emiliano AKS, Souza AP. Efeitos sedativos da detomidina e xilazina em gatos [monografia]. Patos: Universidade Federal de Campina Grande; 2017.

Evans G, Maxwell WMC. Salomon’s Artificial Insemination of Sheep and Goats. Sydney: Butterworths; 1987.

Fyumagwa RD, Bugwesa ZK, Mdaki ML, Mpanduji DG, Kilewo M, Hoare R. Comparison of anaesthesia and cost of two immobilization protocols in free-ranging lions. S Afr J Wildl Res. 2012;42(1):67-70. http://dx.doi.org/10.3957/056.042.0102.

Grove DM, Ramsay EC. Sedative and physiologic effects of orally administered α2-adrenoceptor agonists and ketamine in cats. J Am Vet Med Assoc. 2000;216(12):1929-32. http://dx.doi.org/10.2460/javma.2000.216.1929. PMid:10863590.

Jorge-Neto PN, Luczinski TC, Araújo GR, Salomão Júnior JA, Traldi AS, Santos JAM, Requena LA, Gianni MCM, Deco-Souza T, Pizzutto CS, Baldassarre H. Can jaguar (Panthera onca) ovulate without copulation? Theriogenology. 2020a;147:57-61. http://dx.doi.org/10.1016/j.theriogenology.2020.02.026. PMid:32092606.

Jorge-Neto PN, Silva MCC, Csermak-Júnior AC, Salmão-Júnior JA, Araújo GR, Oliveira G, Leuzinger L, Pizzutto CS, Deco-Souza T. Cryptorchidism in free-living jaguar (Panthera onca): first case report. Anim Reprod. 2020b;17(4):e20200555. http://dx.doi.org/10.1590/1984-3143-ar2020-0555. PMid:33791034.

Jorge Neto PN, Araujo GR, Deco-souza T, Bittencourt RF, Csermak Jr AC, Pizzutto CS, Silva MCC, Salomão Jr JA, Madrigal-Valverde M, Curvelo VP, Gomes MC, Paula TAR. Pharmacological semen collection of Brazilian wild felids. Rev. Bras. Reprodução Anim. 2019;43:704.

Kolata RJ. Laparoscopic ovariohysterectomy and hysterectomy on African lions (Panthera leo) using the ultracision® harmonic scalpel®. J Zoo Wildl Med. 2002;33(3):280-2. http://dx.doi.org/10.1638/1042-7260(2002)033[0280:LOAHOA]2.0.CO;2. PMid:12462496.

Luczinski TC, Araújo GR, Silveira MF, Kirnew MD, Navarrete RA, Salomão-Jr JA, Requena LA, Santos JAM, Koshiyama MH, Pizzutto CS, Jorge-Neto PN. Medetomidine may cause heart murmur in Cougars and Jaguars: case report. J Threat Taxa. 2020;12(14):17000-2. http://dx.doi.org/10.11609/jott.6098.12.14.17000-17002.

Lueders I, Luther I, Scheepers G, van der Horst G. Improved semen collection method for wild felids: urethral catheterization yields high sperm quality in African lions (Panthera leo). Theriogenology. 2012;78(3):696-701. http://dx.doi.org/10.1016/j.theriogenology.2012.02.026. PMid:22538007.

Maddison J, Page S, Church DB. Small Animal Clinical Pharmacology. St Louis: Elsevier; 2008. https://doi.org/10.1016/B978-0-7020-2858-8.X5001-5.

Muir WW, Hubbell JAE. Handbook of Veterinary Anesthesia. St. Louis: Elsevier Health Sciences; 2014.

Prochowska S, Nizański W, Ochota M, Partyka A. Characteristics of urethral and epididymal semen collected from domestic cats-A retrospective study of 214 cases. Theriogenology. 2015;84(9):1565-71. http://dx.doi.org/10.1016/j.theriogenology.2015.08.005. PMid:26359850.

Silva MCC, Araujo GR, Kersul MG, Jorge Neto PN, Aguiar ACB, Miranda FR, Deco-Souza T. Pharmacological semen collection and cryopreservation of the Giant Anteater (Myrmecophaga tridactyla) in the wild. Rev. Bras. Reprod. Anim. 2019;43:705.

Silvatti B, Granato TM, Jorge-Neto PN, Luppi MMCP, Reisfeld LC, Henrique PC, Padilha FLA, Leite RF, Losano JDA, Kawai GKV, Nichi M, Pizzutto CS. Sperm evaluation and morphological description of male genitalia of meerkats (Suricata suricatta). Anim Reprod Sci. 2020;221:106585. http://dx.doi.org/10.1016/j.anireprosci.2020.106585. PMid:32889408.

Sousa PC, Amorim RNL, Lima GL, Paiva ALC, Paula VV, Freitas CIA, Silva AR. Establishment of an anesthetic protocol for semen collection by electroejaculation in six-banded armadillos (Euphractus sexcinctus Linnaeus, 1758). Arq Bras Med Vet Zootec. 2016;68(6):1595-601. http://dx.doi.org/10.1590/1678-4162-8811.

Virtanen R, Savola J-M, Saano V, Nyman L. Characterization of the selectivity, specificity and potency of medetomidine as an α2-adrenoceptor agonist. Eur J Pharmacol. 1988;150(1-2):9-14. http://dx.doi.org/10.1016/0014-2999(88)90744-3. PMid:2900154.

Wetzel RW, Ramsay EC. Comparison of four regimens for intraoral administration of medication to induce sedation in cats prior to euthanasia. J Am Vet Med Assoc. 1998;213(2):243-5. PMid:9676596.

Zambelli D, Prati F, Cunto M, Iacono E, Merlo B. Quality and in vitro fertilizing ability of cryopreserved cat spermatozoa obtained by urethral catheterization after medetomidine administration. Theriogenology. 2008;69(4):485-90. http://dx.doi.org/10.1016/j.theriogenology.2007.10.019. PMid:18082880.
 


Submitted date:
03/02/2021

Accepted date:
06/07/2021

60dc6eb9a9539562c31c1962 animreprod Articles
Links & Downloads

Anim Reprod

Share this page
Page Sections