Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.1590/1984-3143-AR2021-0069
Animal Reproduction (AR)
ORIGINAL ARTICLE

Sperm quality and morphometry characterization of cryopreserved canine sperm in ACP-106c or TRIS

Diego Oliveira Teixeira; Herlon Victor Rodrigues Silva; Bruna Farias Brito; Brenna de Sousa Barbosa; Beatriz Evaristo de Almeida Tabosa; Lúcia Daniel Machado da Silva

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Abstract

Morphological sperm evaluation supported by the morphometry can be used in the determination of the seminal quality and in the investigation of potential extenders. Although there are studies comparing TRIS and ACP extenders, there are no comparative studies between them for the computerized assisted semen analysis (CASA), sperm viability, membrane functionality and sperm morphometry parameters of cryopreserved canine semen. Hence, we aimed to evaluate the effects of ACP-106c and TRIS on post-freezing canine sperm quality. Five dogs were submitted to semen collection twice with one-week interval. The semen was evaluated within the parameters: total motility, vigor, concentration, viability, plasma membrane functionality, morphology and morphometry. In the morphometric evaluation, the morphologically normal sperm was measured as: length, width, area and perimeter of the head and the midpiece, tail length and total length. The parameters of ellipticity, elongation, regularity and roughness were determined. Then, the semen was divided into two aliquots that were diluted in TRIS or ACP-106c, with the addition of egg yolk and glycerol. The diluted semen was refrigerated and frozen. The thawed samples were evaluated. Total motility, viability, sperm membrane functionality and normal morphology reduced after thawing in both extenders (morphology reduced from 89.60 ± 1.3% to 84.40 ± 1.8 and 84.60 ± 1.1% in TRIS and ACP-106c, respectively). However, it did not differ between TRIS and ACP-106c. In the ACP-106c the sperm head defects in cryopreserved semen were higher compared to fresh semen (P < 0.05). For all the morphometric parameters evaluated, there were no differences between fresh and cryopreserved samples (3.70 ± 0.4% vs. 2.30 ± 0.5%). In kinetics, with an interval of one week statistical differences between the extenders were found only in the parameters ALH and LIN (P < 0.05). Regardless of the extender, there were no changes in the morphometric parameters of sperm after thawing.

Keywords

cryopreservation, cryodamage, extenders, morphology, spermatozoa measurement

References

Arruda RP, Ball BA, Gravance CG, Garcia AR, Liu IKM. Effects of extenders and cryoprotectants on stallion sperm head morphometry. Theriogenology. 2002;58(2-4):253-6. http://dx.doi.org/10.1016/S0093-691X(02)00858-0.

Arruda RP, Celeghini ECC, Garcia AR, Santos GDC, Leite TG, Oliveira LZ, Lançoni R, Rodrigues MP. Morfologia espermática de touros: interpretação e impacto na fertilidade. Rev Bras Reprod Anim. 2015;39(1):47-60.

Barbosa BS, Izzo RG, Silva HVR, Nunes TGP, Brito BF, Silva TFP, Silva LDM. Recovery and cryopreservation of epididymal sperm from domestic cat using powdered coconut water (ACP-117c) and TRIS extenders. Cryobiology. 2020;92:103-8. http://dx.doi.org/10.1016/j.cryobiol.2019.11.042. PMid:31770529.

Barbosa BS, Silva HVR, Tabosa BEA, Nunes TGP, Magalhães FF, Silva LDM. Morphological and morphometric characterization of domestic cat epididymal sperm. Reprod Domest Anim. 2019;54(12):1630-6. http://dx.doi.org/10.1111/rda.13572. PMid:31573695.

Cardoso RDCS, Silva AR, Silva LDM. Comparison of two dilution rates on canine semen quality after cryopreservation in a coconut water extender. Anim Reprod Sci. 2006;92(3-4):384-91. http://dx.doi.org/10.1016/j.anireprosci.2005.05.028. PMid:16005585.

Cardoso RDCS, Silva AR, Uchoa DC, Silva LDM. Cryopreservation of canine semen using a coconut water extender with egg yolk and three different glycerol concentrations. Theriogenology. 2003;59(3-4):743-51. http://dx.doi.org/10.1016/S0093-691X(02)01151-2. PMid:12517378.

Cerdeira J, Sánchez-Calabuig MJ, Pérez-Gutiérrez JF, Hijon M, Castaño C, Santiago-Moreno J. Cryopreservation effects on canine sperm morphometric variables and ultrastructure: comparison between vitrification and conventional freezing. Cryobiology. 2020;95:164-70. http://dx.doi.org/10.1016/j.cryobiol.2020.03.007. PMid:32229272.

Chirinea VH, Sicher CC, Lopes MD. Congelamento de sêmen e sua eficiência na inseminação artificial de cães. Rev Bras Reprod Anim. 2013;37(2):164-8.

Colégio Brasileiro de Reprodução Animal – CBRA. Manual para exame andrológico e avaliação de sêmen animal. 3. ed. Belo Horizonte: CBRA; 2013.

Costa LLM, Castelo TS, Souza ALP, Lima GL, Silva AR. Criopreservação de sêmen canino em diluente Tris adicionado de dodecil sulfato de sódio. Rev Bras Reprod Anim. 2013;37(1):53-8.

Cunha DMS, Souza MB, Brito BF, Silva HVR, Ribeiro LR, Xavier FAF Jr, Evangelista JSAM, Pereira LMC, Teixeira DIA. Characterization of seminal parameters, sperm morphometry, micromorphology, and ultrastructure in gray brocket deer (Mazama gouazoubira, Fischer, 1814). Microsc Res Tech. 2021;84(2):313-25. http://dx.doi.org/10.1002/jemt.23590. PMid:32959457.

Esteso MC, Soler AJ, Fernández-Santos MR, Quintero-Moreno AA, Garde JJ. Functional significance of the sperm head morphometric size and shape for determining freezability in iberian red deer (Cervus elaphus hispanicus) epididymal sperm samples. J Androl. 2006;27(5):662-70. http://dx.doi.org/10.2164/jandrol.106.000489. PMid:16728722.

Figueroa E, Lee-Estevez M, Valdebenito I, Watanabe I, Oliveira RPS, Romero J, Castillo RL, Farías JG. Effects of cryopreservation on mitochondrial function and sperm quality in fish. Aquaculture. 2019;511:634190. http://dx.doi.org/10.1016/j.aquaculture.2019.06.004.

García‐Herreros M, Barón FJ, Aparicio IM, Santos AJ, García‐Marín LJ, Gil MC. Morphometric changes in boar spermatozoa induced by cryopreservation. Int J Androl. 2008;31(5):490-8. http://dx.doi.org/10.1111/j.1365-2605.2007.00794.x. PMid:17651399.

Gravance CG, White C, Robertson KR, Champion ZJ, Casey PJ. The effects of cryopreservation on the morphometric dimensions of caprine sperm heads. Anim Reprod Sci. 1997;49(1):37-43. http://dx.doi.org/10.1016/S0378-4320(97)00053-5. PMid:9458948.

Hidalgo M, Rodriguez I, Dorado JM. The effect of cryopreservation on sperm head morphometry in Florida male goat related to sperm freezability. Anim Reprod Sci. 2007;100(1-2):61-72. http://dx.doi.org/10.1016/j.anireprosci.2006.07.003. PMid:16904275.

Maree L, Du Plessis SS, Menkveld R, Van der Horst G. Morphometric dimensions of the human sperm head depend on the staining method used. Hum Reprod. 2010;25(6):1369-82. http://dx.doi.org/10.1093/humrep/deq075. PMid:20400771.

Matos DL, Araújo AA, Roberto IG, Toniolli R. Análise computarizada de espermatozóides: revisão de literatura. Rev Bras Reprod Anim. 2008;32(4):225-32.

Mota AC Fo, Silva HVR, Nunes TGP, Souza MB, Freitas LA, Araújo AA, Silva LDM. Cryopreservation of canine epididymal sperm using ACP-106c and TRIS. Cryobiology. 2014;69(1):17-21. http://dx.doi.org/10.1016/j.cryobiol.2014.04.013. PMid:24824725.

Núñez‐Martinez I, Moran JM, Peña FJ. Do computer‐assisted, morphometric derived sperm characteristics reflect DNA status in canine spermatozoa? Reprod Domest Anim. 2005;40(6):537-43. http://dx.doi.org/10.1111/j.1439-0531.2005.00628.x. PMid:16324080.

Núñez‐Martinez I, Moran JM, Peña FJ. Identification of sperm morphometric subpopulations in the canine ejaculate: do they reflect different subpopulations in sperm chromatin integrity? Zygote. 2007a;15(3):257-66. http://dx.doi.org/10.1017/S0967199407004248. PMid:17637107.

Núñez‐Martínez I, Moran JM, Peña FJ. Sperm indexes obtained using computer‐assisted morphometry provide a forecast of the freezability of canine sperm. Int J Androl. 2007b;30(3):182-9. http://dx.doi.org/10.1111/j.1365-2605.2007.00743.x. PMid:17376220.

Oliveira KG, Miranda SA, Leão DL, Brito AB, Santos RR, Domingues SFS. Semen coagulum liquefaction, sperm activation and cryopreservation of capuchin monkey (Cebus apella) semen in coconut water solution (CWS) and TES-TRIS. Anim Reprod Sci. 2011;123(1-2):75-80. http://dx.doi.org/10.1016/j.anireprosci.2010.11.002. PMid:21129865.

Paz PM, Mata-Campuzano M, Tizado EJ, Alvarez M, Alvarez-Rodríguez M, Herraez P, Anel L. The relationship between ram sperm head morphometry and fertility depends on the procedures of acquisition and analysis used. Theriogenology. 2011;76(7):1313-25. http://dx.doi.org/10.1016/j.theriogenology.2011.05.038. PMid:21798583.

Rahman MA, Park SH, Yu IJ. Effect of monosaccharides in glycerol-free tris extender on reactive oxygen species and apoptosis in dog sperm cryopreservation. Cryo Letters. 2017;38(1):51-7. PMid:28376140.

Ramon M, Pérez-Guzmán MD, Jiménez-Rabadán P, Esteso MC, García-Álvarez O, Maroto-Morales A, Anel-López L, Soler AJ, Fernández-Santos MR, Garde J. Sperm cell population dynamics in ram semen during the cryopreservation process. PLoS One. 2013;8(3):e59189. http://dx.doi.org/10.1371/journal.pone.0059189. PMid:23544054.

Rijsselaere T, Van Soom A, Hoflack G, Maes D, Kruif A. Automated sperm morphometry and morphology analysis of canine semen by the Hamilton-Thorne analyser. Theriogenology. 2004;62(7):1292-306. http://dx.doi.org/10.1016/j.theriogenology.2004.01.005. PMid:15325556.

Robert MA, Jayaprakash G, Pawshe M, Tamilmani T, Sathiyabarathi M. Collection and evaluation of canine semen-a review. Int J Sci. 2016;5(3):1586-95.

Ros-Santaella JL, Domínguez-Rebolledo AE, Garde JJ. Sperm flagellum volume determines freezability in red deer spermatozoa. PLoS One. 2014;9(11):e112382. http://dx.doi.org/10.1371/journal.pone.0112382. PMid:25380133.

Salazar JL Jr, Teague SR, Love CC, Brinsko SP, Blanchard TL, Varner DD. Effect of cryopreservation protocol on postthaw characteristics of stallion sperm. Theriogenology. 2011;76(3):409-18. http://dx.doi.org/10.1016/j.theriogenology.2011.02.016. PMid:21496899.

Sieme H, Oldenhof H, Wolkers WF. Mode of action of cryoprotectants for sperm preservation. Anim Reprod Sci. 2016;169:2-5. http://dx.doi.org/10.1016/j.anireprosci.2016.02.004. PMid:26936658.

Silva AR, Cardoso RCS, Silva LDM. Comparison between powder coconut water (ACP®) and Tris as extenders for canine semen cryopreservation. Braz J Vet Res Anim Sci. 2006;43(6):767-74. http://dx.doi.org/10.11606/issn.1678-4456.bjvras.2006.26555.

Silva HVR, Magalhães FF, Ribeiro LR, Souza ALP, Freitas CIA, Oliveira MF, Silva AR, Silva LDM. Morphometry, morphology and ultrastructure of ring‐tailed coati sperm (Nasua nasua Linnaeus, 1766). Reprod Domest Anim. 2015;50(6):945-51. http://dx.doi.org/10.1111/rda.12613. PMid:26446691.

Silva HVR, Nunes TGP, Mota AC Fo, Pinto JN, Silva AR, Silva LDM. Adição de hidroxitolueno butilado (BHT) no diluidor ACP-106c para congelação de sêmen canino. Cienc Anim Bras. 2018;9(0):1-9. http://dx.doi.org/10.1590/1809-6891v19e-45896.

Soler C, Alambiaga A, Martí MA, García-Molina A, Valverde A, Contell J, Campos M. Dog sperm head morphometry: its diversity and evolution. Asian J Androl. 2017;19(2):149-53. http://dx.doi.org/10.4103/1008-682X.189207. PMid:27751991.

Tremori TM, Monteiro-Toma CD, Rodello L, Bicudi SD. Avaliação da heterogeneidade espermática de carneiros por meio da análise morfométrica computadorizada não automatizada utilizando-se preparação úmida e coloração de Karras. Rev Bras Reprod Anim. 2014;38(3):154-8.

Uchoa DC, Silva TFP, Mota AC Fo, Silva LD. M. Criopreservação de sêmen e inseminação artificial em cães. Ciênc Anim. 2012;22(1):132-42.

Urbano M, Ortiz I, Dorado J, Hidalgo M. Identification of sperm morphometric subpopulations in cooled‐stored canine sperm and its relation with sperm DNA integrity. Reprod Domest Anim. 2017;52(3):468-76. http://dx.doi.org/10.1111/rda.12935. PMid:28220615.

Valverde A, Arenán H, Sancho M, Contell J, Yániz J, Fernández A, Soler C. Morphometry and subpopulation structure of Holstein bull spermatozoa: variations in ejaculates and cryopreservation straws. Asian J Androl. 2016;18(6):851-7. http://dx.doi.org/10.4103/1008-682X.187579. PMid:27678464.

Yániz JL, Palacín I, Vicente-Fiel S, Sánchez-Nadal JA, Santolaria P. Sperm population structure in high and low field fertility rams. Anim Reprod Sci. 2015;156:128-34. http://dx.doi.org/10.1016/j.anireprosci.2015.03.012. PMid:25835571.

Yildiz C, Kaya A, Aksoy M, Tekeli T. Influence of sugar supplementation of the extender on motility, viability and acrossomal integrity of dog spermatozoa during freezing. Theriogenology. 2000;54(4):579-85. http://dx.doi.org/10.1016/S0093-691X(00)00373-3. PMid:11071132.
 


Submitted date:
07/20/2021

Accepted date:
08/03/2022

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