Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.1590/1984-3143-AR2021-0083
Animal Reproduction (AR)
ORIGINAL ARTICLE

Sperm cryopreservation of Prochilodus brevis using different concentrations of non-permeable cryoprotectants

Thais Maia Torres; Priscila Silva de Almeida-Monteiro; Renata Vieira do Nascimento; Vanessa Alves Pereira; Yasmim Maia Ferreira; Jessica Sales Lobato; Romulo Roberto Ribeiro Pinheiro; Yara Silvino Sales; Assis Rubens Montenegro; Carminda Sandra Brito Salmito-Vanderley

Downloads: 2
Views: 102

Abstract

The action of substances with non-permeable cryoprotectant potential, besides glucose, has not yet been studied for the species Prochilodus brevis. The objective of this work was to evaluate the action of four non-permeable cryoprotectants on this species sperm cryopreservation. Five pools were cryopreserved in a solution of 5% glucose and 10% dimethyl sulfoxide (Me2SO) associated or not (control) with cryoprotectants egg yolk (5, 10 or 12%), soy lecithin (2.5, 7.5 or 10%), sucrose (5, 10 or 20%) and lactose (5, 8 or 15%). After thawing, samples were evaluated for sperm kinetics (total motility, motility duration, velocities, and wobble - WOB), morphology and membrane and DNA integrity. The treatments containing egg yolk improved significantly (P<0.05) results when compared the control for the membrane integrity parameter. When compared to other treatments, egg yolk, at any concentration, presented higher results (P<0.05) for membrane integrity, total motility, curvilinear velocity (VCL) and average path velocity (VAP) parameters. Egg yolk also showed the best results for WOB, but it did not differ from 5% and 8% lactose and 5% and 20% sucrose. Soy lecithin had the lowest percentages of morphologically normal sperm (P<0.05), while the other treatments did not differ from each other. There was no difference regarding DNA integrity data. Thus, 5% egg yolk is indicated as a non-permeable cryoprotectant for P. brevis, in association with 5% glucose and 10% Me2SO.

Keywords

cryopreservation, extender, fish, semen

References

Aires VA, Hinsch KD, Mueller-Schloesser F, Bogner K, Mueller-Schloesser S, Hinsch E. In vitro and in vivo comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of bovine semen. Theriogenology. 2003;60(2):269-79. http://dx.doi.org/10.1016/S0093-691X(02)01369-9 PMid:12749940.

Aisen EG, Medina VH, Venturino A. Cryopreservation and post-thawed fertility of ram semen frozen in different trehalose concentrations. Theriogenology. 2002;57(7):1801-8. http://dx.doi.org/10.1016/S0093-691X(02)00653-2 PMid:12041684.

Almeida-Monteiro PS, Araújo MSO, Pinheiro RRR, Lopes JT, Ferreira YM, Montenegro AR, Maciel MAPM, Vanderley CSBS. Influence of vitamins C and E on the quality of cryopreserved semen Prochilodus brevis (Prochilodontidae, Teleostei). Semina: Ciênc Agrár. 2017;38(4):2669-80. http://dx.doi.org/10.5433/1679-0359.2017v38n4SUPLp2669.

Almeida-Monteiro PS, Pinheiro RRR, Araújo MSO, Sales YS, Nascimento RV, Nunes LT, Pereira VA, Montenegro AR, Maciel MAPM, Vanderley CSBS. Sperm vitrification of Prochilodus brevis using Powder Coconut Water (ACP‐104) in association with different cryoprotectant concentrations. Aquacult Res. 2020;51(11):4565-74. http://dx.doi.org/10.1111/are.14802.

Araújo AS. Variação temporal da frequência de captura e sazonalidade reprodutiva de Prochilodus cearensis Steindachener,1911 (Characiformes, Prochilodontidae) no açude Itans, Caíco/RN [dissertation]. Natal: Universidade Federal do Rio grande do Norte; 1998. Portuguese.

Blom E. A one-minute live-dead sperm stain by means of eosin-nigrosin. Fertil Steril. 1950;1(2):176-7. http://dx.doi.org/10.1016/S0015-0282(16)30125-X.

Bucak MN, Keskin N, Taspinar M, Çoyan K, Bașpinar N, Cenariu MC, Bilgili A, Öztürk C, Kurșunlu AN. Raffinose and hypotaurine improve the post-thawed Merino ram sperm parameters. Cryobiology. 2013;67(1):34-9. http://dx.doi.org/10.1016/j.cryobiol.2013.04.007 PMid:23644017.

Carneiro PCF, Azevedo HC, Santos JP, Maria AN. Cryopreservation of tambaqui (Colossoma macropomum) semen: extenders, cryoprotectants, dilution ratios and freezing methods. Cryo Letters. 2012;33(5):385-93. PMid:23224371.

Carolsfeld J, Godinho HP, Zaniboni E Fo, Harvey BJ. Cryopreservation of sperm in Brazilian migratory fish conservation. J Fish Biol. 2003;63(2):472-89. http://dx.doi.org/10.1046/j.1095-8649.2003.00170.x.

Chellappa S, Bueno RMX, Chellappa T, Chellappa NT, Val VMFA. Reproductive seasonality of the fish fauna and limnoecology of semi-arid Brazilian reservoirs. Limnologica. 2009;39(4):325-9. http://dx.doi.org/10.1016/j.limno.2009.06.003.

Cosson J. Fish sperm physiology: structure, factors regulating motility, and motility evaluation. In: Bozkurt Y, editor. Biological research in aquatic science. London: IntechOpen; 2019. http://dx.doi.org/10.5772/intechopen.85139

Dziewulska K, Rzemieniecki A, Czerniawski R, Domagała J. Post-thawed motility and fertility from Atlantic salmon (Salmo salar L.) sperm frozen with four cryodiluents in straws or pellets. Theriogenology. 2011;76(2):300-11. http://dx.doi.org/10.1016/j.theriogenology.2011.02.007 PMid:21496895.

Fauvel C, Suquet M, Cosson J. Evaluation of fish sperm quality. J Appl Ichthyology. 2010;26(5):636-43. http://dx.doi.org/10.1111/j.1439-0426.2010.01529.x.

Felizardo VO, Mello RA, Murgas LDS, Andrade ES, Drumond MM, Rosa PV. Effect of cryopreservant combinations on the motility and morphology of curimba (Prochilodus lineatus) sperm. Anim Reprod Sci. 2010;122(3-4):259-63. http://dx.doi.org/10.1016/j.anireprosci.2010.08.020 PMid:20888715.

Felizardo VO, Melo RA, Murgas LDS, Andrade ES, Navarro RD, Freitas RTF. Optimization of artificial propagation in piracanjuba fish Brycon orbignyanus using criopreserved semen. Cryo Letters. 2016;37(5):330-4. PMid:27925000.

Fernandez JL, Muriel L, Goyanes V, Segrelles E, Gosálvez J, Enciso M, LaFromboise M, Jonge C. Simple determination of human sperm DNA fragmentation with an improved sperm chromatin dispersion test. Fertil Steril. 2005;84(4):833-42. http://dx.doi.org/10.1016/j.fertnstert.2004.11.089 PMid:16213830.

Figueroa E, Valdebenito I, Merino O, Ubilla A, Risopatrón J, Farias JG. Cryopreservation of Atlantic salmon Salmo salar sperm: effects on sperm physiology. J Fish Biol. 2016;89(3):1537-50. http://dx.doi.org/10.1111/jfb.13052. PMid:27406003.

Fowler A, Toner M. Cryo-injury and biopreservation. Ann N Y Acad Sci. 2006;1066(1):119-35. http://dx.doi.org/10.1196/annals.1363.010 PMid:16533923.

Garcia VJA, Espinosa JA, Martínez JG, Carrasco SCP. Insemination of bocachico fish (Prochilodus magdalenae) with fresh or cryopreserved semen: effect of spermatozoa/oocyte ratio. Rev Colomb Cienc Pecu. 2015;28:347-55.

Golshahi K, Aramli MS, Nazari RM, Habibi E. Disaccharide supplementation of extenders is an effective means of improving the cryopreservation of semen in sturgeon. Aquaculture. 2018;486:261-5. http://dx.doi.org/10.1016/j.aquaculture.2017.12.045.

Gurgel LL, Verani JR, Chellappa S. Reproductive ecology of Prochilodus brevis an endemic fish from the semiarid region of Brazil. ScientificWorldJournal. 2012;2012:810532. http://dx.doi.org/10.1100/2012/810532 PMid:22629205.

Ingermann RL, Schultz CLF, Kanuga MK, Wilson-Leedy JG. Metabolism of motile zebrafish sperm. Comp Biochem Physiol A Mol Integr Physiol. 2011;158(4):461-7. http://dx.doi.org/10.1016/j.cbpa.2010.12.008 PMid:21147245.

Kampschmidt RF, Mayer DT, Herman HA. Lipid and lipoprotein constituents of egg yolk in the resistance and storage of bull spermatozoa. J Dairy Sci. 1953;36(7):733-42. http://dx.doi.org/10.3168/jds.S0022-0302(53)91553-7.

Kavamoto ET, Barnabé VH, Campos BES, Talmelli EFA. Anormalidades morfológicas nos espermatozóides do curimbatá (Prochilodus scrofa). Bol Inst Pesca. 1999;25:61-6.

Kopeika E, Kopeika J, Zhang T. Cryopreservation of fish sperm. Methods Mol Biol. 2007;368:203-17. http://dx.doi.org/10.1007/978-1-59745-362-2_14 PMid:18080472.

Liu QH, Li J, Xiao ZZ, Ding FH, Yu DD, Xu XZ. Use of computer-assisted sperm analysis (CASA) to evaluate the quality of cryopreserved sperm in red seabream (Pagrus major). Aquaculture. 2007;263(1-4):20-5. http://dx.doi.org/10.1016/j.aquaculture.2006.11.017.

Lopes JT. Lecitina de soja e antioxidantes na composição de meios de congelação espermática de tambaqui (Colossoma macropomum) [thesis]. Fortaleza: Universidade Estadual do Ceará; 2019. Portuguese.

Magnotti C, Cerqueira V, Lee-Estevez M, Farias JG, Valdebenito I, Figueroa E. Cryopreservation and vitrification of fish semen: a review with special emphasis on marine species. Rev Aquacult. 2016;10(1):15-25. http://dx.doi.org/10.1111/raq.12145.

Maria AN, Azevedo HC, Carneiro PCF. Protocolo para criopreservação do sêmen de Tambaqui (Colossoma macropomum). Comunicado Técnico. 2011;112:1-5.

Martínez JG, Pardo SC. Effect of freezing and thawing rates on sperm motility in Bocachico Prochilodus magdalenae (Pisces, Characiformes). Rev Mvz Cordoba. 2013;18(1):3295-303. http://dx.doi.org/10.21897/rmvz.191.

Mazur P. Freezing of living cells: mechanisms and implications. Am J Physiol. 1984;247(3):C125-42. http://dx.doi.org/10.1152/ajpcell.1984.247.3.C125 PMid:6383068.

McIntyre PB, Jones LE, Flecker AS, Vanni MJ. Fish extinctions alter nutrient recycling in tropical freshwaters. Proc Natl Acad Sci USA. 2007;104(11):4461-6. http://dx.doi.org/10.1073/pnas.0608148104 PMid:17360546.

Mendiburu F, Yaseen M. Agricolae: statistical procedures for agricultural research (1.4.0; p. 1). Vienna: R Foundation for Statistical Computing; 2020 [cited 2021 Aug 08]. Available from: https://myaseen208.github.io/agricolae/

Miliorini AB, Murgas LDS, Rosa PV, Oberlender G, Pereira GJM, Costa DV. A morphological classification proposal for curimba (Prochilodus lineatus) sperm damages after cryopreservation. Aquacult Res. 2011;42(2):177-87. http://dx.doi.org/10.1111/j.1365-2109.2010.02575.x.

Moussa M, Martinet V, Trimeche A, Tainturier D, Anton M. Low density lipoproteins extracted from hen egg yolk by an easy method: cryoprotective effect on frozen–thawed bull semen. Theriogenology. 2002;57(6):1695-706. http://dx.doi.org/10.1016/S0093-691X(02)00682-9 PMid:12035979.

Muchlisin ZA, Sarah PI, Aldila DF, Eriani K, Hasri I, Batubara AS, Nur FM, Mustaqim M, Muthmainnah CR, Abinawanto A, Wilkes M. Effect of Dimethyl sulfoxide (DMSO) and egg yolk on sperm motility, fertility and hatching rates of depik Rasbora tawarensis (Pisces: Cyprinidae) eggs after short‐term cryopreservation. Aquacult Res. 2020;51(4):1700-5. http://dx.doi.org/10.1111/are.14516.

Nascimento AF, Maria AN, Pessoa NO, Carvalho MAM, Viveiros ATM. Out-of-season sperm cryopreservation in diferente media of the Amazonian freshwater fish pirapitinga (Piaractus brachypomus). Anim Reprod Sci. 2010;118(2-4):324-9. http://dx.doi.org/10.1016/j.anireprosci.2009.07.002 PMid:19679412.

Nimalaratne C, Lopes-Lutz D, Schieber A, Wu J. Free aromatic amino acids in egg yolk show antioxidant properties. Food Chem. 2011;129(1):155-61. http://dx.doi.org/10.1016/j.foodchem.2011.04.058.

Nunes LT, Oliveira MS, Lopes JT, Almeida-Monteiro PS, Nascimento RV, Pereira VA, Ferreira YM, Montenegro AR, Pinheiro JU, Salmito-Vanderley CSB. Capacidade fecundante do sêmen congelado de Prochilodus brevis. Acta Sci Vet. 2019;47:1665.

Nunes LT, Oliveira MS, Lopes JT, Souza MEM, Pinheiro RRR, Campello CC, Salmito-Vanderley CSB. Cryopreservation of Prochilodus brevis semen: freezing media and thawing rates. Semina: Ciênc Agrár. 2016;37(3):1643-54. http://dx.doi.org/10.5433/1679-0359.2016v37n3p1643.

R Core Team. R: A language and environment for statistical computing. Vienna: R Foundation for Statistical Computing; 2020 [cited 2021 Aug 08]. Available from: https://www.r-project.org/

Rusco G, Di Iorio M, Gibertoni PP, Esposito S, Penserini M, Roncarati A, Cerolini S, Iaffaldano N. Optimization of sperm cryopreservation protocol for mediterranean brown trout: a comparative study of non-permeating cryoprotectants and thawing rates In Vitro and In Vivo. Animals. 2019;9(6):304. http://dx.doi.org/10.3390/ani9060304 PMid:31159204.

Sanches EA, Okawara RY, Caneppele D, Toledo CPR, Bombardelli RA, Romagosa E. Sperm characteristics of Steindachneridion parahybae (Steindachner, 1877) throughout 112 h of storage at four temperatures. J Appl Ichthyology. 2015;31:79-88. http://dx.doi.org/10.1111/jai.12728.

Shimoda E. Análise e criopreservação do sêmen da piabanha Brycon insignis Steindachner, 1877 (Pisces, Characidae) [thesis]. Campos dos Goytacazes: Universidade Estadual do Norte Fluminense Darcy Ribeiro; 2004. Portuguese.

Signorell A. DescTools: tools for descriptive statistics. Vienna: R Foundation for Statistical Computing; 2021.

Venables W, Ripley B. Modern applied statistics with S. 4th ed. New York: Springer; 2002 [cited 2021 Aug 08]. Available from: https://www.stats.ox.ac.uk/pub/MASS4

Viveiros ATM, Godinho HP. Sperm quality and cryopreservation of Brazilian freshwater fish species: a review. Fish Physiol Biochem. 2009;35(1):137-50. http://dx.doi.org/10.1007/s10695-008-9240-3 PMid:19189240.

Watson PF. The causes of reduced fertility with cryopreserved semen. Anim Reprod Sci. 2000;60-61:481-92. http://dx.doi.org/10.1016/S0378-4320(00)00099-3 PMid:10844218.

Yildiz C, Bozkurt Y, Yavas I. An evaluation of soybean lecithin as an alternative to avian egg yolk in the cryopreservation of fish sperm. Cryobiology. 2013;67(1):91-4. http://dx.doi.org/10.1016/j.cryobiol.2013.05.008 PMid:23727066.
 


Submitted date:
08/08/2021

Accepted date:
01/11/2022

61fc21cba953954229008e93 animreprod Articles
Links & Downloads

Anim Reprod

Share this page
Page Sections