Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.1590/1984-3143-AR2021-0084
Animal Reproduction (AR)
ORIGINAL ARTICLE

Synchronization of follicular wave emergence does not improve embryonic yield in superovulated ewes

Oscar Oliveira Brasil; Nathalia Hack Moreira; Fábia Fernanda Cardoso de Barros da Conceição; Paula Lorena Grangeira Souto; Cleidson Manoel Gomes da Silva; Alexandre Floriani Ramos

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Abstract

The present study aimed to investigate the effects of a combination of progesterone with different doses of E-17β on following end points: (1) ovarian follicular dynamics and emergence of a new follicular wave, and (2) superovulatory response and embryo yield. In Experiment 1, 28 ewes were randomly divided into four groups (n = 7) to receive either 2.0 mg, 1.0 mg, 0.5 mg or none E-17β one day after insertion of a progesterone device. The different doses of estradiol similarly delayed the moment of follicular emergence (overall mean = 3.1 ± 1.0 days vs. control group = 0.86 ± 1.0 days; P < 0.01), but the emergence of the new wave showed greater synchronization with the 0.5 mg dosage of E-17β. In Experiment 2, sixty-two donor ewes received an internal progesterone release device (day -1) for 7 d and 1 d after the insertion of this device (day 0) were allocated randomly to receive 0.5 mg of E-17β or only the vehicle (control group). Superstimulation was initiated on day 3 with the administration of 133 mg of pFSH in eight decreasing doses. Contrary to expectations, the protocol with the administration of 0.5 mg E-17β did not improve the percentage of donors with > 2 CL, the number of CL and the production of embryos (P > 0.05). It was concluded that the combination of progesterone and 0.5 mg E-17β was more efficient in synchronizing the emergence of the new follicular wave, however this approach seems to be unnecessary in ewe’s superovulation programs.

Keywords

embryos, estradiol-17β, recruitment, follicle, superovulation

References

Alvares CA, Stape JL, Sentelhas PC, Goncalves JLD, Sparovek G. Koppen’s climate classification map for Brazil. Meteorol Z. 2013;22(6):711-28. http://dx.doi.org/10.1127/0941-2948/2013/0507.

Barrett DM, Bartlewski PM, Duggavathi R, Davies KL, Huchkowsky SL, Epp T, Rawlings NC. Synchronization of follicular wave emergence in the seasonally anestrous ewe: the effects of estradiol with or without medroxyprogesterone acetate. Theriogenology. 2008;69(7):827-36. http://dx.doi.org/10.1016/j.theriogenology.2007.12.010. PMid:18258293.

Bartlewski PM, Alexander BD, Rawlings NC, Barrett D, King WA. Ovarian responses, hormonal profiles and embryo yields in anoestrous ewes superovulated with Folltropin-V after pretreatment with medroxyprogesterone acetate-releasing vaginal sponges and a single dose of oestradiol-17beta. Reprod Domest Anim. 2008a;43(3):299-307. http://dx.doi.org/10.1111/j.1439-0531.2007.00894.x. PMid:18069949.

Bartlewski PM, Alexander BD, King WA. Ovarian and endocrine determinants of superovulatory responses in anestrous ewes. Small Rumin Res. 2008b;75(2-3):210-6. http://dx.doi.org/10.1016/j.smallrumres.2007.11.003.

Bartlewski PM, Beard AP, Cook SJ, Chandolia RK, Honaramooz A, Rawlings NC. Ovarian antral follicular dynamics and their relationships with endocrine variables throughout the oestrous cycle in breeds of sheep differing in prolificacy. J Reprod Fertil. 1999;115(1):111-24. http://dx.doi.org/10.1530/jrf.0.1150111. PMid:10341729.

Bartlewski PM, Seaton P, Franco Oliveira ME, Kridli RT, Murawski M, Schwarz T. Intrinsic determinants and predictors of superovulatory yields in sheep: circulating concentrations of reproductive hormones, ovarian status, and antral follicular blood flow. Theriogenology. 2016;86(1):130-43. http://dx.doi.org/10.1016/j.theriogenology.2016.04.024. PMid:27173957.

Bo GA, Adams GP, Caccia M, Martínez M, Pierson RA, Mapletoft RJ. Ovarian follicular wave emergence after treatment with progestogen and estradiol in cattle. Anim Reprod Sci. 1995;39(3):193-204. http://dx.doi.org/10.1016/0378-4320(95)01389-H.

Bo GA, Adams GP, Pierson RA, Tribulo HE, Caccia M, Mapletoft RJ. Follicular wave dynamics after estradiol-17-beta treatment of heifers with or without a progestogen implant. Theriogenology. 1994;41(8):1555-69. http://dx.doi.org/10.1016/0093-691X(94)90821-Y.

Bó GA, Baruselli PS, Moreno D, Cutaia L, Caccia M, Tríbulo R, Tríbulo H, Mapletoft RJ. The control of follicular wave development for self-appointed embryo transfer programs in cattle. Theriogenology. 2002;57(1):53-72. http://dx.doi.org/10.1016/S0093-691X(01)00657-4. PMid:11775981.

Brasil OO, Moreira NH, Santos G, Silva BDM, Mariante AS, Ramos AF. Superovulatory and embryo yielding in sheep using increased exposure time to progesterone associated with a GnRH agonist. Small Rumin Res. 2016;136:54-8. http://dx.doi.org/10.1016/j.smallrumres.2016.01.005.

Bungartz L, Niemann H. Assessment of the presence of a dominant follicle and selection of dairy cows suitable for superovulation by a single ultrasound examination. J Reprod Fertil. 1994;101(3):583-91. http://dx.doi.org/10.1530/jrf.0.1010583. PMid:7966012.

Cognié Y, Baril G, Poulin N, Mermillod P. Current status of embryo technologies in sheep and goat. Theriogenology. 2003;59(1):171-88. http://dx.doi.org/10.1016/S0093-691X(02)01270-0. PMid:12499027.

Cognie Y. State of the art in sheep-goat embryo transfer. Theriogenology. 1999;51(1):105-16. http://dx.doi.org/10.1016/S0093-691X(98)00235-0. PMid:10729066.

Duggavathi R, Bartlewski PM, Barrett DM, Bagu E, Rawlings NC. Short- and long-term effects of unilateral ovariectomy in sheep: causative mechanisms. Biol Reprod. 2008;78(3):490-6. http://dx.doi.org/10.1095/biolreprod.107.064188. PMid:18046013.

Duggavathi R, Bartlewski PM, Barrett DM, Rawlings NC. Use of high-resolution transrectal ultrasonography to assess changes in numbers of small ovarian antral follicles and their relationships to the emergence of follicular waves in cyclic ewes. Theriogenology. 2003;60(3):495-510. http://dx.doi.org/10.1016/S0093-691X(03)00041-4. PMid:12763163.

Ferreira AM, Viana JHM, Camargo LSA, Sá WF, Pereira PAC. Ovarian follicular population during estrous cycle in Gir breed (Bos indicus) cows. Rev Bras Zootec. 2004;33(6, Suppl. 1):1689-94. http://dx.doi.org/10.1590/S1516-35982004000700007.

Garcia A, Salaheddine M. Effect of oestrous synchronization with estradiol 17beta and progesterone on follicular wave dynamics in dairy heifers. Reprod Domest Anim. 2001;36(6):301-7. http://dx.doi.org/10.1046/j.1439-0531.2001.00306.x. PMid:11928925.

Ginther OJ, Wiltbank MC, Fricke PM, Gibbons JR, Kot K. Selection of the dominant follicle in cattle. Biol Reprod. 1996;55(6):1187-94. http://dx.doi.org/10.1095/biolreprod55.6.1187. PMid:8949873.

González-Bulnes A, Baird DT, Campbell BK, Cocero MJ, García-García RM, Inskeep EK, López-Sebastián A, McNeilly AS, Santiago-Moreno J, Souza CJ, Veiga-López A. Multiple factors affecting the efficiency of multiple ovulation and embryo transfer in sheep and goats. Reprod Fertil Dev. 2004;16(4):421-35. http://dx.doi.org/10.1071/RD04033. PMid:15315741.

González-Bulnes A, Santiago-Moreno J, Cocero MJ, Lopez-Sebastian A. Effects of FSH commercial preparation and follicular status on follicular growth and superovulatory response in Spanish Merino ewes. Theriogenology. 2000;54(7):1055-64. http://dx.doi.org/10.1016/S0093-691X(00)00414-3. PMid:11131324.

González-Bulnes A, Santiago-Moreno J, Cocero MJ, Souza CJ, Groome NP, Garcia-Garcia RM, Lopez-Sebastian A, Baird DT. Measurement of inhibin A and follicular status predict the response of ewes to superovulatory FSH treatments. Theriogenology. 2002;57(4):1263-72. http://dx.doi.org/10.1016/S0093-691X(01)00723-3. PMid:12013446.

Kakar MA, Maddocks S, Lorimer MF, Kleemann DO, Rudiger SR, Hartwich KM, Walker SK. The effect of peri-conception nutrition on embryo quality in the superovulated ewe. Theriogenology. 2005;64(5):1090-103. http://dx.doi.org/10.1016/j.theriogenology.2004.12.017. PMid:16125553.

Lagares MA, Varago FC, Moustacas VS, Gheller VA, Nicolino RR, Borges I, Henry M. Effect of season and frequency of embryo collections on superovulatory response and embryo recovery in Santa Inês hair sheep. Small Rumin Res. 2021;201:106441. http://dx.doi.org/10.1016/j.smallrumres.2021.106441.

Mapletoft RJ, Bennett Steward K, Adams GP. Recent advances in the superovulation in cattle. Reprod Nutr Dev. 2002;42(6):601-11. http://dx.doi.org/10.1051/rnd:2002046. PMid:12625424.

Martínez MF, Adams GP, Kastelic JP, Bergfelt DR, Mapletoft RJ. Induction of follicular wave emergence for estrus synchronization and artificial insemination in heifers. Theriogenology. 2000;54(5):757-69. http://dx.doi.org/10.1016/S0093-691X(00)00388-5. PMid:11101036.

Menchaca A, Vilariño M, Crispo M, Castro T, Rubianes E. New approaches to superovulation and embryo transfer in small ruminants. Reprod Fertil Dev. 2010;22(1):113-8. http://dx.doi.org/10.1071/RD09222. PMid:20003852.

Mossa F, Duffy P, Naitana S, Lonergan P, Evans AC. Association between numbers of ovarian follicles in the first follicle wave and superovulatory response in ewes. Anim Reprod Sci. 2007;100(3-4):391-6. http://dx.doi.org/10.1016/j.anireprosci.2006.10.016. PMid:17101245.

Naqvi SMK, Gulyani R, Joshi A, Das GK, Mittal JP. Effect of dietary regimens on ovarian response and embryo production of sheep in tropics. Small Rumin Res. 2002;46(2-3):167-71. http://dx.doi.org/10.1016/S0921-4488(02)00193-1.

Rubianes E, Ibarra D, Ungerfeld R, Carbajal B, Castro T. Superovulatory response in anestrous ewes is affected by the presence of a large follicle. Theriogenology. 1995;43(2):465-72. http://dx.doi.org/10.1016/0093-691X(94)00039-W. PMid:16727638.

Schiewe MC, Fitz TA, Brown JL, Stuart LD, Wildt DE. Relationship of oestrus synchronization method, circulating hormones, luteinizing hormone and prostaglandin F-2 alpha receptors and luteal progesterone concentration to premature luteal regression in superovulated sheep. J Reprod Fertil. 1991;93(1):19-30. http://dx.doi.org/10.1530/jrf.0.0930019. PMid:1920289.

Seekallu SV, Toosi BM, Zeigler A, Rawlings NC. Effects of estradiol and progesterone on circulating LH and FSH secretion, and ovarian antral follicle growth in anestrous ewes. Small Rumin Res. 2010;91(2-3):178-85. http://dx.doi.org/10.1016/j.smallrumres.2010.03.012.

Toosi BM, Seekallu SV, Barrett DM, Davies KL, Duggavathi R, Bagu ET, Rawlings NC. Characteristics of peaks in serum concentrations of follicle-stimulating hormone and estradiol, and follicular wave dynamics during the interovulatory interval in cyclic ewes. Theriogenology. 2010;73(9):1192-201. http://dx.doi.org/10.1016/j.theriogenology.2009.11.005. PMid:20347126.

Veiga-Lopez A, Dominguez V, Souza CJ, Garcia-Garcia RM, Ariznavarreta C, Tresguerres JA, McNeilly AS, González-Bulnes A. Features of follicle-stimulating hormone-stimulated follicles in a sheep model: keys to elucidate embryo failure in assisted reproductive technique cycles. Fertil Steril. 2008a;89(5, Suppl):1328-37. http://dx.doi.org/10.1016/j.fertnstert.2007.02.034. PMid:17604028.

Veiga-Lopez A, Encinas T, McNeilly AS, González-Bulnes A. Timing of preovulatory LH surge and ovulation in superovulated sheep are affected by follicular status at start of the FSH treatment. Reprod Domest Anim. 2008b;43(1):92-8. PMid:18199264.

Veiga-Lopez A, González-Bulnes A, Garcia-Garcia RM, Dominguez V, Cocero MJ. The effects of previous ovarian status on ovulation rate and early embryo development in response to superovulatory FSH treatments in sheep. Theriogenology. 2005;63(7):1973-83. http://dx.doi.org/10.1016/j.theriogenology.2004.09.055. PMid:15823353.

Viñoles C, Meikle A, Forsberg M. Accuracy of evaluation of ovarian structures by transrectal ultrasonography in ewes. Anim Reprod Sci. 2004;80(1-2):69-79. http://dx.doi.org/10.1016/S0378-4320(03)00141-6. PMid:15036516.
 


Submitted date:
08/30/2021

Accepted date:
12/03/2021

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