Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.1590/1984-3143-AR2021-0112
Animal Reproduction (AR)
ORIGINAL ARTICLE

Influence of eCG and reproductive management in the resynchronization of ovulation in dairy goats

Isabel Oliveira Cosentino; Mário Felipe Alvares Balaro; Polyanne Martins da Silva; Augusto Ryonosuke Taira; Juliana Dantas Rodrigues Santos; Ana Clara Sarzedas Ribeiro; Bruna Ramalho Rigaud de Figueiredo; Marta Maria Campos Pereira da Costa; Bruno Ribeiro Vieira; Felipe Zandonadi Brandão

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Abstract

Resynchronization protocols have been proposed as a way of shortening females’ unproductive time in the flock, with good results in cattle and sheep. In goats, initial studies have shown that a second progestogen device inserted before luteolysis and pregnancy diagnosis does not interfere with the corpus luteum lifespan or functionality. This study aimed to evaluate the follicular growth, ovulation pattern and pregnancy rate after insertion of a second and new progestogen device for resynchronizing, with or without equine Chorionic Gonadotrophin (eCG), submitted to natural mating (NM) or artificial insemination (AI) to propose a viable resynchronization protocol for dairy goats. A total of 38 multiparous Saanen goats underwent a short-term progesterone protocol [six days exposed to medroxyprogesterone acetate (MAP) intravaginal sponges + 200 IU eCG and 0.12 mg of cloprostenol sodium on the 5th day + 0.025 mg of lecirelin 34 hours after sponge withdrawal] and, on day 16th after the ovulation, received a new MAP device which was retained until day 21. At this moment females were split into four groups: GeCG+NM – 100 IU eCG with NM; GSal+NM – saline solution with NM; GeCG+AI – 100 IU eCG with AI; and GSal+AI – saline solution with AI. Ultrasound scans were performed every 12 h from sponge withdrawal (day 21) until 108 h after sponge withdrawal (day 25) for follicular dynamics evaluation, at 240 h (day 31) for assessing the presence of active corpus luteum, and on day 60 for pregnancy diagnosis. No differences were found regarding ovulation time, synchronization and follicle size. However, GeCG+NM presented a greater estrus manifestation rate (100%) and pregnancy rate (62.5%) when compared to GSal+AI. In conclusion, resynchronization protocols in dairy goats may present satisfactory results.

Keywords

follicle dynamics, ultrasound, artificial insemination, natural mating

References

Alvarado-Espino AS, Meza-Herrera CA, Carrillo E, González-Álvarez VH, Guillen-Muñoz JM, Ángel-García O, Mellado M, Véliz-Deras FG. Reproductive outcomes of Alpine goats primed with progesterone and treated with human chorionic gonadotropin during the anestrus-to-estrus transition season. Anim Reprod Sci. 2016;167:133-8. http://dx.doi.org/10.1016/j.anireprosci.2016.02.019. PMid:26944772.

Andrade ABP, Morais MCC, Rangel PSC, Oliveira MEF, Souza-Fabjan JMG, Fonseca JF. Effect of eCG in a short-term synchronization treatment on ovarian status, estrus synchrony, and ovulation in dairy goats managed under tropical conditions. Trop Anim Health Prod. 2021;53(2):246. http://dx.doi.org/10.1007/s11250-021-02699-y. PMid:33821404.

Balaro MFA, de Mello SGV, da Silva Santos A, Cavalcanti LM, Almosny NRP, Fonseca JF, Brandão FZ. Reproductive seasonality in Saanen goats kept under tropical conditions. Trop Anim Health Prod. 2019;51(2):345-53. http://dx.doi.org/10.1007/s11250-018-1696-2. PMid:30267190.

Bartolome JA, Sozzi A, McHale J, Swift K, Kelbert D, Archbald LF, Thatcher WW. Resynchronization of ovulation and timed insemination in lactating dairy cows: III. Administration of GnRH 23 days post AI and ultrasonography for nonpregnancy diagnosis on day 30. Theriogenology. 2005;63(6):1643-58. http://dx.doi.org/10.1016/j.theriogenology.2004.07.018. PMid:15763108.

Bó GA, de la Mata JJ, Baruselli PS, Menchaca A. Alternative programs for synchronizing and resynchronizing ovulation in beef cattle. Theriogenology. 2016;86(1):388-96. http://dx.doi.org/10.1016/j.theriogenology.2016.04.053. PMid:27180326.

Bottino MP, Simões LMS, Silva LACL, Girotto RW, Scandiuzzi LA Jr, Massoneto JPM, Baruselli PS, Souza JC, Sales JNS. Effects of eCG and FSH in timed artificial insemination treatment regimens on estrous expression and pregnancy rates in primiparous and multiparous Bos indicus cows. Anim Reprod Sci. 2021;228:106751. http://dx.doi.org/10.1016/j.anireprosci.2021.106751. PMid:33866258.

Bukar MM, Yusoff R, Haron AW, Dhaliwal GK, Khan MA, Omar MA. Estrus response and follicular development in Boer does synchronized with flugestone acetate and PGF2α or their combination with eCG or FSH. Trop Anim Health Prod. 2012;44(7):1505-11. http://dx.doi.org/10.1007/s11250-012-0095-3. PMid:22461200.

Castro T, Rubianes E, Menchaca A, Rivero A. Ovarian dynamics, serum estradiol and progesterone concentrations during the interovulatory interval in goats. Theriogenology. 1999;52(3):399-411. http://dx.doi.org/10.1016/S0093-691X(99)00138-7. PMid:10734375.

Chemineau P, Baril G, Leboeuf B, Maurel MC, Roy F, Pellicer-Rubio M, Malpaux B, Cognie Y. Implications of recent advances in reproductive physiology for reproductive management of goats. J Reprod Fertil Suppl. 1999;54:129-42. PMid:10692850.

Cosentino IO, Balaro MFA, Arashiro EKN, Santos JDR, Carvalho ABS, Clariget RP, Ungerfeld R, Brandão FZ. Hormonal protocols for early resynchronization of ovulation in ewes: the use of progestagens, eCG, and inclusion of early pregnancy diagnosis with color Doppler ultrasound. Theriogenology. 2019;133:113-8. http://dx.doi.org/10.1016/j.theriogenology.2019.04.033. PMid:31082747.

Cosentino IO, Balaro MFA, Leal FSC, Bade ALC, Barbosa LFC, Gonçalves FM, Cortat de Souza PR, Pereira PVS, Bartlewski PM, Brandão FZ. Ovarian activity in dairy Saanen goats subjected to a short-term ovulation induction protocol and a single injection of lecirelin (GnRH analog) given 28 h or 34 h after progestin pre-treatment. Small Rumin Res. 2020a;191:106214. http://dx.doi.org/10.1016/j.smallrumres.2020.106214.

Cosentino IO, Balaro MFA, Leal FSC, Barbosa LFC, Gonçalves FM, Felizardo GF, Monteiro M No, Brandão FZ. Early resynchronization protocols for goats: progestogens can be used prior to an early pregnancy diagnosis without affecting corpus luteum functionality. Reprod Domest Anim. 2020b;55(11):1655-59. http://dx.doi.org/10.1111/rda.13830.

Cosentino IO, Brandão FZ, Pinto PHN, Clariget RP, Ungerfeld R. Fixed timed artificial insemination and early resynchronization of ovulation in different categories of ewes in grazing system: response of multiparous, nulliparous, and lactating ewes during the breeding season. Livest Sci. 2021;247:104461. http://dx.doi.org/10.1016/j.livsci.2021.104461.

Fonseca JF, Alvim GP, Souza-Fabjan JMG, Oliveira MEF, Brair VL, Brandão FZ, Facó O. Reproductive features and use of an anti-inflammatory drug in estrus-induced dairy goats artificially inseminated in a standing position with cervix immobilization. Reprod Biol. 2017;17(3):268-73. http://dx.doi.org/10.1016/j.repbio.2017.07.002. PMid:28736292.

Ginther OJ, Kot K. Follicular dynamics during the ovulatory season in goats. Theriogenology. 1994;42(6):987-1001. http://dx.doi.org/10.1016/0093-691X(94)90121-X. PMid:16727603.

Gonzalez de Bulnes A, Santiago Moreno J, Gomez-Brunet A, Inskeep EK, Townsend EC, Lopez-Sebastian A. Follicular dynamics during the oestrous cycle in dairy goats. Anim Sci. 1999;68(3):547-54. http://dx.doi.org/10.1017/S1357729800050578.

Honnens A, Niemann H, Herzog K, Paul V, Meyer HHD, Bollwein H. Relationships between ovarian blood flow and ovarian response to eCG-treatment of dairy cows. Anim Reprod Sci. 2009;113(1-4):1-10. http://dx.doi.org/10.1016/j.anireprosci.2008.05.077. PMid:18619746.

Hosseini A, Niasari-Naslaji A, Vojgani M, Gharagozloo F. Effect of time of eCG administration on the fate of ovarian follicle in Holstein heifers. Iran J Vet Res. 2018;19(1):15-21. PMid:29805457.

Kilkenny C, Browne WJ, Cuthill IC, Emerson M, Altman DG. Improving bioscience research reporting: the ARRIVE guidelines for reporting animal research. PLoS Biol. 2010;8(6):e1000412. http://dx.doi.org/10.1371/journal.pbio.1000412. PMid:20613859.

Köeppen W. Climatologia: con un estudio de los climas de la tierra. Ciudad de México: Fondo de Cultura Economica; 1948.

Kumar TS, Farman M, Nandi S, Girish Kumar V, Gupta PSP. Biochemical constituents of ovarian follicular fluid in ruminants and their significance in follicle and oocyte development. J Vet Sci Med Diagn. 2015;4:3. http://dx.doi.org/4172/2325-9590.1000159.

Kumar VG, Tripathi SK, Farman M, Nandi S. Ovarian intrafollicular fluid factors and their roles in follicle and ovum development in ruminants. J Infertil Reprod Biol. 2014;2:124-35.

Maia ALRS, Brandão FZ, Souza-Fabjan JMG, Balaro MFA, Oliveira MEF, Facó O, Fonseca JF. Reproductive parameters of dairy goats after receiving two doses of d-cloprostenol at different intervals. Anim Reprod Sci. 2017;181:16-23. http://dx.doi.org/10.1016/j.anireprosci.2017.02.013. PMid:28385398.

Mattioli M, Barboni B, Turriani M, Galeati G, Zannoni A, Castellani G, Berardinelli P, Scapolo PA. Follicle activation involves vascular endothelial growth factor production and increased blood vessel extension. Biol Reprod. 2001;65(4):1014-9. http://dx.doi.org/10.1095/biolreprod65.4.1014. PMid:11566721.

Miranda VO, Oliveira FC, Dias JH, Vargas Júnior SF, Goularte KL, Sá Filho MF, Sá Filho OG, Baldassarre H, Vieira AD, Lucia T Jr, Gasperin BG. Estrus resynchronization in ewes with unknown pregnancy status. Theriogenology. 2018;106:103-7. http://dx.doi.org/10.1016/j.theriogenology.2017.10.019. PMid:29049921.

Muñoz AL, Aroña RM, Bedos M, Hernández H, Keller M, Chemineau P, Delgadillo JA. Presence of a sexually active goat buck enhances ovulation occurrence in seasonally anestrous does after ovulation and luteolysis induction in hormonally-treated goats in seasonal anestrus. Anim Reprod Sci. 2019;211:106209. http://dx.doi.org/10.1016/j.anireprosci.2019.106209. PMid:31785631.

Palhão MP, Ribeiro AC, Martins AB, Guimarães CRB, Alvarez RD, Seber MF, Fernandes CAC, Neves JP, Viana JHM. Early resynchronization of non-pregnant beef cows based in corpus luteum blood flow evaluation 21 days after Timed-AI. Theriogenology. 2020;146:26-30. http://dx.doi.org/10.1016/j.theriogenology.2020.01.064. PMid:32036057.

Pugliesi G, Bisinotto DZ, Mello BP, Lahr FC, Ferreira CA, Melo GD, Bastos MR, Madureira EH. A novel strategy for resynchronization of ovulation in Nelore cows using injectable progesterone (P4) and P4 releasing devices to perform two timed inseminations within 22 days. Reprod Domest Anim. 2019;54(8):1149-54. http://dx.doi.org/10.1111/rda.13475. PMid:31134689.

Riesenberg S, Meinecke-Tillmann S, Meinecke B. Ultrasonic survey of follicular development following superovulation with a single application of pFSH, eCG or hMG in goats. Small Rumin Res. 2001;40(1):83-93. http://dx.doi.org/10.1016/S0921-4488(00)00211-X. PMid:11259879.

Roy F, Combes B, Vaiman D, Cribiu EP, Pobel T, Delétang F, Combarnous Y, Guillou F, Maurel MC. Humoral immune response to equine chorionic gonadotropin in ewes: association with major histocompatibility complex and interference with subsequent fertility. Biol Reprod. 1999;61(1):209-18. http://dx.doi.org/10.1095/biolreprod61.1.209. PMid:10377051.

Sani RN, Farzaneh N, Moezifar M, Seifi HA, Tabatabei AA. Evaluation of five resynchronization methods using different combinations of PGF2α, GnRH, estradiol and an intravaginal progesterone device for insemination in Holstein cows. Anim Reprod Sci. 2011;124(1-2):1-6. http://dx.doi.org/10.1016/j.anireprosci.2011.02.013. PMid:21396790.

Suiter J. Body condition scoring of sheep and goats. Addis Ababa, Ethiopia: ESGPIP; 1994. Technical Bulletin; 69.

Universidade Federal de Viçosa – UFV. SAEG: Sistema para Análises Estatísticas. Viçosa: Fundação Arthur Bernardes; 2007.

Véliz-Deras FG, Meza-Herrera CA, Mellado M, Contreras-Villarreal V, Gaytán-Alemán LR, Guillén-Muñoz JM. The Effect of P4 + eCG Estrus Induction Protocol during the Deep and the Transition Anestrous Period on the Reproductive Performance of Crossbred Dairy Goats. Biology (Basel). 2020;9(10):311. http://dx.doi.org/10.3390/biology9100311. PMid:32987953.
 


Submitted date:
10/26/2021

Accepted date:
08/31/2022

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