Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.1590/1984-3143-AR2021-0114
Animal Reproduction (AR)
ORIGINAL ARTICLE

Assessment of coconut water added to Numida meleagris egg yolk as cryoprotectant for goat semen

Laércio Fontinele Bandeira de Macêdo; Letícia Soares de Araújo Texeira; Clarissa de Castro e Braga; Kenney de Paiva Porfírio; Sara Camila da Silveira Costa; Louis Henrique Miyauchi Silva; Wcleuden Matias Nascimento; Francisca Kelly dos Santos Silva; Nildene Silva Andrade Bandeira; Rômulo José Vieira; Ana Lys Bezerra Barradas Mineiro; Cristiane Clemente de Mello Salgueiro; José Ferreira Nunes; Janaina de Fátima Saraiva Cardoso; Ney Rômulo de Oliveira Paula

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Abstract

Effects were assessed of the dilutants TRIS and ACP - 101c® with the addition of different guinea fowl (Numida meleagris) egg yolk concentrations. Fifteen ejaculates were collected from five goats of the Anglo Nubian breed. The ejaculates were pooled and then divided into 12 groups, two control groups (GC1 TRIS, with 2.5% Gallus gallus domesticus hen egg yolk GOGD), (GC2 Control Group ACP - 101c®, with the addition of 2.5% Gallus gallus domesticus hen egg yolk GOGD) and ten experimental groups (EG), containing TRIS and ACP added with different concentrations of egg yolk from guinea hen (Numida meleagris) (TRIS 2,5% GONM; TRIS 5% GONM; TRIS 10% GONM; TRIS 15% GONM; TRIS 20% GONM; ACP® 2,5% GONM; ACP® 5% GONM; ACP® 10% GONM; ACP® 15% GONM; ACP® 20% GONM). Then cryopreservation was carried out and the samples stored in liquid nitrogen (-196 °C). After seven days, the samples were thawed and assessed for spermatic kinetics, immunofluorescence and sperm morphology. Analysis of GOMN by the CASA system showed that the various parameters were similar to those of GOGD (P>0.05). The membrane integrity, mitochondrial potential and the acrosome were not influenced by the treatment (P>0.05) nor by the dilutant used for cryopreservation (P>0.05). The spermatic morphology was also preserved by the different GOGD and GONM concentrations in the ACP® and TRIS dilutants, with no statistically significant differences (P<0.05). It was concluded that Numida meleagris egg yolk, as external membrane cryoproctant added to the dilutants ACP-101c® and TRIS, improved goat semen quality.

Keywords

goat, semen, cryoprotectant, egg yolk

References

Adeyeye EI. Characteristic composition of guinea fowl (Numida Meleagris) eGG. Int J Pharma Bio Sci. 2010;1(2):1-9.

Alvarez-Suarez JM, Tulipani S, Díaz D, Estevez Y, Romandini S, Giampieri F, Damiani E, Astolfi P, Bompadre S, Battino M.Antioxidant and antimicrobial capcity of several monofloral Cuban honeys and their correlation with color, polyphenol content and other chemical compounds. Food Chem Toxicol. 2011;48(8-9):2490-9. http://dx.doi.org/10.1016/j.fct.2010.06.021. PMid:20558231.

Amidi F, Farshad A, Khor AK. Effects of cholesterol-loaded cyclodextrin during freezing step of cryopreservation with TCGY extender containing bovine serum albumin on quality of goat spermatozoa. Cryobiology. 2010;61(1):94-9. http://dx.doi.org/10.1016/j.cryobiol.2010.05.006. PMid:20561514.

Anand M, Baghel G, Yadav S. Effect of egg yolk concentration and washing on sperm quality following cryopreservation in barbari buck sêmen. J Appl Anim Res. 2017;45(1):560-5. http://dx.doi.org/10.1080/09712119.2016.1232265.

Andrabi SMH, Ansari MS, Ullah N, Anwar M, Mehmood A, Akhter S. Duck egg yolk in extender improves the freezability of bufalo bull spermatozoa. Anim Reprod Sci. 2008;104(2-4):427-33. http://dx.doi.org/10.1016/j.anireprosci.2007.07.003. PMid:17709214.

Arangasamy A, Venkata Krishnaiah M, Manohar N, Selvaraju S, Guvvala PR, Soren NM, Reddy IJ, Roy KS, Ravindra JP. Advancement of puberty and enhancement of seminal characteristics by supplementation of trace minerals to bucks. Theriogenology. 2018;110:182-91. http://dx.doi.org/10.1016/j.theriogenology.2018.01.008. PMid:29407900.

Association of Official Analytical Chemists – AOAC. Official methods of analysis (16th ed. ). Arlington: AOAC; 2006.

Azerêdo GA, Esper CR, Resende KT. Evaluation of plasma membrane integrity of frozen thawed goat spermatozoa with or without seminal plasma. Small Rumin Res. 2001;41(3):257-63. http://dx.doi.org/10.1016/S0921-4488(01)00189-4.

Bashir L, Ossai P, Shittu O, Abubakar A, Caleb T. Comparison of the nutritional value of egg yolk and egg albumin from domestic chicken, guinea fowl and hybrid chicken. Am J Exp Agric. 2015;6(5):310-6. http://dx.doi.org/10.9734/AJEA/2015/15068.

Bathgate R, Maxwell WM, Evans G. Studies on the effect supplementing boar semen cryopreservation media with different avian egg yolk types on in vitro post-thaw sperm quality. Reprod Domest Anim. 2006;41(1):68-73. http://dx.doi.org/10.1111/j.1439-0531.2006.00623.x. PMid:16420332.

Bispo CAS, Pugliesi G, Palhão MP, Coelho PGB, Ker PG, Rodrigues MT, Carvalho GR. Características in vitro e fertilidade do sêmen caprino armazenado a 5° C por 24 horas utilizando duas concentrações de gema de ovo no diluente. Cienc Anim Bras. 2011;12(4):653-60. http://dx.doi.org/10.5216/cab.v12i4.13115.

Câmara TS, Sousa A Jr, Barçante FPS, Silva JHL, Sousa MS, Machado AAC, Salgueiro CCM, Montenegro AR, Nunes JF. Comparação da qualidade seminal de caprinos das raças Canindé e Alpina Britânica no Nordeste brasileiro. Arq Bras Med Vet Zootec. 2019;71(4):1260-8. http://dx.doi.org/10.1590/1678-4162-10297.

Cardoso RC, Silva AR, Uchoa DC, da Silva LD. Cryopreservation of canine semen using a coconut water extender with egg yolk and three different glycerol concentrations. Theriogenology. 2003;59(3-4):743-51. http://dx.doi.org/10.1016/S0093-691X(02)01151-2. PMid:12517378.

Carvalho JM, Maia GA, Sousa PHM, Maia GA Jr. Água de coco: propriedades nutricionais, funcionais e processamento. Semina: Ciênc Agrár. 2006;27(3):437-52. http://dx.doi.org/10.5433/1679-0359.2006v27n3p437.

Colégio Brasileiro de Reprodução Animal. Manual de exame andrológico (2. ed). Belo Horizonte: Colégio Brasileiro de Reprodução Animal; 2013.

Dorado J, Rodríguez I, Hidalgo M. Cryopreservation of goat spermatozoa: comparison of two freezing extenders based on post-thaw sperm quality and fertility rates after artificial insemination. Theriogenology. 2007;68(2):168-77. http://dx.doi.org/10.1016/j.theriogenology.2007.04.048. PMid:17532460.

Ferrari S, Barnabe VH. Effect of two kinds of diluents and two freezing methods on caprine semen quality. Braz J Vet Res Anim Sci. 1999;36(4):199-204. https://doi.org/10.1590/S1413-95961999000400006.

Forouzanfar M, Sharafi M, Hosseini SM, Ostadhosseini S, Hajian M, Hosseini L, Abedi P, Nili N, Rahmani HR, Nasr-Esfahani MH. In vitro comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of ram semen. Theriogenology. 2010;73(4):480-7. http://dx.doi.org/10.1016/j.theriogenology.2009.10.005. PMid:20022626.

Guthrie HD, Welch GR. Determination of intracellular reactive oxygen species and high mitochondrial membrane potential in Percoll-treated viable boar sperm using fluorescence-activated flow cytometry. J Anim Sci. 2006;84(8):2089-100. http://dx.doi.org/10.2527/jas.2005-766. PMid:16864869.

Harrison RAP, Vickers SE. Use of fluorescent probes to assess membrane integrity in mammalian spermatozoa. J Reprod Fertil. 1990;88(1):343-352. http://dx.doi.org/10.1530/jrf.0.0880343. PMid:1690300.

Hidalgo M, Rodriguez I, Dorado J. Influence of staining and sampling procedures on goat sperm morphometric using the sperm class analyzer. Theriogenology. 2006;66(4):996-1003. http://dx.doi.org/10.1016/j.theriogenology.2006.02.039. PMid:16620924.

Hunes R, Webb G. Use of chichen or chukar egg yolk with two cryoprotectants for preservation of stallion sêmen. Anim Reprod Sci. 2006;94(1-4):62-3. http://dx.doi.org/10.1016/j.anireprosci.2006.03.023.

Layek SS, Mohanty TK, Kumaresan A, Parks JE. Cryopreservation of bull semen: evolution from egg yolk based to soybean based extenders. Anim Reprod Sci. 2016;172:1-9. http://dx.doi.org/10.1016/j.anireprosci.2016.04.013. PMid:27509873.

Machado VP, Nunes JF, Araújo AA, Fernández DRP, Cordeiro MA, Medeiros CHN, Medeiros ALN, Monteiro AWU. Fertilidade após inseminação artificial intra-cervical ou laparoscópica intra-uterina de ovelhas utilizando diluidores à base de água de coco. Braz J Vet Res Anim Sci. 2006;43(suppl.):43-9. http://dx.doi.org/10.11606/issn.1678-4456.bjvras.2006.26534.

Narwade BM, Mohanty TK, Bhakat M, Rahim A. Goat semen Cryopreservation using egg yolk and soya based extenders containing trehalose. Indian J Anim Sci. 2017;87:851-5.

Oliveira RV, Nunes JF, Salgueiro CCM, Cavalcante JMM, Brasil OO, Moura AAAN. Avaliação de espermatozoides caprinos congelados em meio à base de água de coco em pó (ACP-101) ou TRIS. Arq Bras Med Vet Zootec. 2011;6(6):1295-302. http://dx.doi.org/10.1590/S0102-09352011000600003.

Purdy PH. A review on goat sperm cryopreservation. Small Rumin Res. 2006;63(3):215-25. http://dx.doi.org/10.1016/j.smallrumres.2005.02.015.

Qin Y, Yang S, Xu J, Xia C, Li X, An L, Tian J. Deep insemination with sex-sorted Cashmere goat sperm processed in the presence of antioxidants. Reprod Domest Anim. 2018;53(1):11-9. http://dx.doi.org/10.1111/rda.13045. PMid:29205543.

Roth TL, Weiss RB, Buff JL, Bush LM, Wildt DE, Bush M. Heterologous in vitro fertilization and sperm capacitation in an endangered African antelope, the Scimitar-Horned Oryx (Oryx dammah). Biol Reprod. 1998;58(2):475-82. http://dx.doi.org/10.1095/biolreprod58.2.475. PMid:9475404.

Rufino MSM, Alves RE, de Brito ES, Pérez-Jiménez J, Saura-Calixto F, Mancini-Filho J. Bioactive compounds and antioxidant capacities of 18 non-traditional tropical fruits from Brazil. Food Chem. 2010;121(4):996-1002. http://dx.doi.org/10.1016/j.foodchem.2010.01.037.

Salgueiro CCM, Mateos-Rex E, Sampaio NJC, Nunes JF. Utilização de diferentes diluentes e métodos de congelamento do sêmen de bodes da raça Murciano-Granadina. Rev Bras Reprod Anim. 2003;27:625-30.

Salgueiro CCM, Nunes JF, Oliveira KPL, Vieira VE, Gondim JM, Mareosrex E. Utilização de diluentes à base de água de coco in natura e em pó na inseminação artificial programada de cabras. Rev Bras Reprod Anim. 2002;5:96-8.

Santiago-Moreno J, Esteso MC, Castaño C, Toledano-Díaz A, Delgadillo JA, López-Sebastián A. Seminal plasma removal by density-gradient centrifugation is superior for goat sperm preservation compared with classical sperm washing. Anim Reprod Sci. 2017;181:141-50. http://dx.doi.org/10.1016/j.anireprosci.2017.04.002. PMid:28416376.

Silva AR, Cardoso RCS, Silva LDM. Comparação entre a água de coco em pó (ACP) e o Tris como diluidores na criopreservação do sêmen de cães. Braz J Vet Res Anim Sci. 2006;43(6):767-74. http://dx.doi.org/10.11606/issn.1678-4456.bjvras.2006.26555.

Verstegen J, Iguer-Ouada M, Onclin K. Computer assisted semen analyzers in andrology research and veterinary practice. Theriogenology. 2002;57(1):149-79. http://dx.doi.org/10.1016/S0093-691X(01)00664-1. PMid:11775967.

Watson PF. Recent developments and concepts in the cryopreservation of spermatozoa and assessment of their post-thawing function. Reprod Fertil Dev. 1995;7(4):871-91. http://dx.doi.org/10.1071/RD9950871. PMid:8711221.

Watt B, Merrill AL. Composition of foods: raw, processed, prepared. Washington: Consumer and Food Economics Research Division; 1963.
 


Submitted date:
11/01/2021

Accepted date:
09/05/2022

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