Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.1590/1984-3143-AR2022-0025
Animal Reproduction (AR)
ORIGINAL ARTICLE

Combination of nanoparticle green tea extract in tris-egg yolk extender and 39 °c thawing temperatures improve the sperm quality of post-thawed Kacang goat semen

Imam Mustofa; Suherni Susilowati; Tri Wahyu Suprayogi; Yudit Oktanella; Djoko Agus Purwanto; Adeyinka Oye Akintunde

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Abstract

Kacang goats are small ruminants produced by low-income households in smallholder and farm to reduce poverty and prevent undernutrition. Studies to find a cryopreservation protocol for Kacang goat semen are expected to multiplication of genetically superior animals selected by the paternal lineage. This study evaluated the effect of thawing temperature and supplementation of the green tea extract nanoparticle in skim milk-egg yolk (SM-EY) extender on post-thaw sperm quality of Kacang goat semen. Six ejaculates of Kacang goat were diluted in SM-EY supplemented or not (control group) with 0.001 mg/mL NPs GTE. The diluted semen was packaged with 0.25 mL straws (insemination dose: 60x106 sptz/mL) and cryopreserved. Then, six samples of the control group and NPs GTE groups were thawed at 37°C or 39°C sterile water for 30 s and submitted to sperm quality evaluations. The sperm viability, motility, and intact of the plasma membrane (IPM) were higher (p<0.05) in NPs GTE group than control group. In contrast, the NPs GTE group presented lower (p<0.05) malondialdehyde levels and sperm DNA fragmentation (SDF) compared with the control group. The catalase levels were not significantly different (p > 0.05) between the control and NPs GTE groups. Thawing at 39°C resulted in higher (p<0.05) sperm viability, motility, and IPM than thawing at 37°C. However, thawing at 39°C group presented lower (p<0.05) malondialdehyde levels compared with thawing at 37°C. SDF and catalase levels were similar (p>0.05) between thawing at 37°C and thawing at 37°C. In conclusion, supplementation of 0.001 mg/mL of NPs GTE in SM-EY extender and thawing temperature of 39°C resulted in a better quality of frozen-thawed Kacang goat semen.

Keywords

catalase, frozen-thawed sperm, small holder and farm, small ruminant, sperm function

References

Abdelnour SA, Alagawany M, Hashem NM, Farag MR, Alghamdi ES, Hassan FU, Bilal RM, Elnesr SS, Dawood MAO, Nagadi SA, Elwan HAM, ALmasoudi AG, Attia YA. Nanominerals: fabrication methods, benefits and hazards, and their applications in ruminants with special reference to selenium and zinc nanoparticles. Animals. 2021;11(7):1916. http://dx.doi.org/10.3390/ani11071916. PMid:34203158.

Ahmed H, Jahan S, Ullah H, Ullah F, Salman MM. The addition of resveratrol in tris citric acid extender ameliorates post-thaw quality parameters, antioxidant enzymes levels, and fertilizing capability of buffalo (Bubalus bubalis) bull spermatozoa. Theriogenology. 2020;152:106-13. http://dx.doi.org/10.1016/j.theriogenology.2020.04.034. PMid:32388038.

Behzadi S, Serpooshan V, Tao W, Hamaly MA, Alkawareek MY, Dreaden EC, Brown D, Alkilany AM, Farokhzad OC, Mahmoudi M. Cellular uptake of nanoparticles: journey inside the cell. Chem Soc Rev. 2017;46(14):4218-44. http://dx.doi.org/10.1039/C6CS00636A. PMid:28585944.

Bergstein-Galan TG, Bicudo LC, Rodello L, Weiss RR, Bicudo SD. Sperm membrane integrity and stability after selection of cryopreserved ovine semen on colloidal solutions. Andrologia. 2018;50(2):e12867. http://dx.doi.org/10.1111/and.12867. PMid:28758695.

Bibov MY, Kuzmin AV, Alexandrova AA, Chistyakov VA, Dobaeva NM, Kundupyan OL. Role of the reactive oxygen species induced DNA damage in human spermatozoa dysfunction. AME Med J. 2018;3:19. http://dx.doi.org/10.21037/amj.2018.01.06.

Borah BK, Deka BC, Biswas RK, Chakravarty P, Deori S, Sinha S, Ahmed K. Effect of thawing methods on frozen semen quality of yak (Poephagus grunniens L.) bulls. Vet World. 2015;8(7):831-4. http://dx.doi.org/10.14202/vetworld.2015.831-834. PMid:27047161.

Demirhan A, Tekin K, Daşkin A, Uysal O. Assessment of morphological features of bull semen thawed at various temperatures and periods of time with CASA. Kocatepe Vet J. 2020;13(3):286-93. http://dx.doi.org/10.30607/kvj.722620.

Dutta S, Majzoub A, Agarwal A. Oxidative stress and sperm function: a systematic review on evaluation and management. Arab J Urol. 2019;17(2):87-97. http://dx.doi.org/10.1080/2090598X.2019.1599624. PMid:31285919.

Esteves SC, Santi D, Simoni M. An update on clinical and surgical interventions to reduce sperm DNA fragmentation in infertile men. Andrology. 2020;8(1):53-81. http://dx.doi.org/10.1111/andr.12724. PMid:31692293.

Evans HC, Dinh TTN, Hardcastle ML, Gilmore AA, Ugur MR, Hitit M, Jousan FD, Nicodemus MC, Memili E. Advancing semen evaluation using lipidomics. Front Vet Sci. 2021;8:601794. http://dx.doi.org/10.3389/fvets.2021.601794. PMid:33937366.

Falchi L, Khalil WA, Hassan M, Marei WFA. Perspectives of nanotechnology in male fertility and sperm function. Int J Vet Sci Med. 2018;6(2):265-9. http://dx.doi.org/10.1016/j.ijvsm.2018.09.001. PMid:30564607.

Feng C, Zhu Z, Bai W, Li R, Zheng Y, Tian X, Wu D, Lu H, Wang Y, Zeng W. Proline protects boar sperm against oxidative stress through proline dehydrogenase-mediated metabolism and the amine structure of pyrrolidine. Animals. 2020;10(9):1549. http://dx.doi.org/10.3390/ani10091549. PMid:32883027.

Fujii J, Imai H. Redox reactions in mammalian spermatogenesis and the potential targets of reactive oxygen species under oxidative stress. Spermatogenesis. 2014;4(2):e979108. http://dx.doi.org/10.4161/21565562.2014.979108. PMid:26413390.

Gale I, Gil L, Malo C, González N, Martínez F. Effect of Camellia sinensis supplementation and increasing holding time on quality of cryopreserved boar semen. Andrologia. 2015;47(5):505-12. http://dx.doi.org/10.1111/and.12293. PMid:24909203.

Gautier C, Aurich C. “Fine feathers make fine birds” - the mammalian sperm plasma membrane lipid composition and effects on assisted reproduction. Anim Reprod Sci. 2022;246:106884. http://dx.doi.org/10.1016/j.anireprosci.2021.106884. PMid:34776291.

Gungor SS, Ata A, Inac ME. Effects of trehalose and catalase on the viability and kinetic parameters of cryopreserved ram semen. Acta Sci Vet. 2018;46:1-7.

Hadwan MH. Simple spectrophotometric assay for measuring catalase activity in biological tissues. BMC Biochem. 2018;19(1):7. http://dx.doi.org/10.1186/s12858-018-0097-5. PMid:30075706.

Hereng YA, Selan YN, Amalo FA. Parameter fisiologi kambing Kacang (Capra aegagrus hircus) di desa Nunkurus kecamatan Kupang Timur kabupaten Kupang. J Vet Nusantara [Internet]. 2019 Dec [cited 2022 Sep 14];2(2):161-9. Available from: http://ejurnal.undana.ac.id/index.php/jvn/article/view/1843.

INSA [homepage on the Internet]. Jakarta: Indonesian National Standard Agency; 2014. INSA frozen semen-part 3: goat and sheep; 2014 [cited 2019 Dec 30]. Available from: https://docplayer.info/30304410-Semen-beku-bagian-3-kambing-dan-domba.html.

Jeevanandam J, Barhoum A, Chan YS, Dufresne A, Danquah MK. Review on nanoparticles and nanostructured materials: history, sources, toxicity and regulations. Beilstein J Nanotechnol. 2018;9:1050-74. http://dx.doi.org/10.3762/bjnano.9.98. PMid:29719757.

Joudeh N, Linke D. Nanoparticle classification, physicochemical properties, characterization, and applications: a comprehensive review for biologists. J Nanobiotechnology. 2022;20(1):262. http://dx.doi.org/10.1186/s12951-022-01477-8. PMid:35672712.

Khalil WA, El-Harairy MA, Zeidan AEB, Hassan MAE, Mohey-Elsaeed O. Evaluation of bull spermatozoa during and after cryopreservation: structural and ultrastructural insights. Int J Vet Sci Med. 2018;6(Suppl 1):S49-56. http://dx.doi.org/10.1016/j.ijvsm.2017.11.001. PMid:30761321.

Khalil WA, El-Harairy MA, Zeidan AEB, Hassan MAE. Impact of selenium nano-particles in semen extender on bull sperm quality after cryopreservation. Theriogenology. 2019;126:121-7. http://dx.doi.org/10.1016/j.theriogenology.2018.12.017. PMid:30551018.

Le MT, Nguyen TTT, Nguyen TT, Nguyen TV, Nguyen TAT, Nguyen QHV, Cao TN. Does conventional freezing affect sperm DNA fragmentation? Clin Exp Reprod Med. 2019;46(2):67-75. http://dx.doi.org/10.5653/cerm.2019.46.2.67. PMid:31181874.

Li Z, Wang H, Yuan C, Lu P, Zhou Y, Lu W, Zhao J, Liu H, Wang J. Epigallocatechin 3-gallate improves the quality of bull semen cryopreservation. Andrologia. 2022;54(1):e14310. http://dx.doi.org/10.1111/and.14310. PMid:34750852.

Lone SA. Possible mechanisms of cholesterol-loaded cyclodextrin action on sperm during cryopreservation. Anim Reprod Sci. 2018;192:1-5. http://dx.doi.org/10.1016/j.anireprosci.2018.03.009. PMid:29559192.

Martin-Hidalgo D, Bragado MJ, Batista AR, Oliveira PF, Alves MG. Antioxidants and male fertility: from molecular studies to clinical evidence. Antioxidants. 2019;8(4):89. http://dx.doi.org/10.3390/antiox8040089. PMid:30959797.

Mustofa I, Susilowati S, Wurlina W, Hernawati T, Oktanella Y. Green tea extract increases the quality and reduced DNA mutation of post-thawed Kacang buck sperm. Heliyon. 2021;7(3):e06372. http://dx.doi.org/10.1016/j.heliyon.2021.e06372. PMid:33732926.

Narwade BM, Mohanty TK, Bhakat M, Rahim A. Goat semen cryopreservation using egg yolk and soya based diluters containing trehalose. Indian J Anim Sci. 2017;87:851-5.

Nicolae D, Stela Z, Dragomir C, Hortanse AA. Effect of thawing time and temperature variation on the quality of frozenthawed ram semen. Rom Biotechnol Lett [Internet]. 2014 [cited 2022 Dec 22];19:8935-40. Available from: https://www.rombio.eu/vol19nr1/4%20lucr%206%20Dobrin%20Nicolae%20fin%20rec%2012.pdf.

Papas M, Arroyo L, Bassols A, Catalán J, Bonilla-Correal S, Gacem S, Yeste M, Miró J. Activities of antioxidant seminal plasma enzymes (SOD, CAT, GPX and GSR) are higher in jackasses than in stallions and are correlated with sperm motility in jackasses. Theriogenology. 2019a;140:180-7. http://dx.doi.org/10.1016/j.theriogenology.2019.08.032. PMid:31479834.

Papas M, Catalán J, Fernandez-Fuertes B, Arroyo L, Bassols A, Miró J, Yeste M. Specific activity of superoxide dismutase in stallion seminal plasma is related to sperm cryotolerance. Antioxidants. 2019b;8(11):539. http://dx.doi.org/10.3390/antiox8110539. PMid:31717586.

Penitente-Filho JM, Oliveira FA, Jimenez CR, Carrascal E, Dias JC, Oliveira GD, Silveira RG, Silveira CO, Torres CA. Association of vitamin E with rapid thawing on goat semen. ScientificWorldJournal. 2014;2014:964172. http://dx.doi.org/10.1155/2014/964172. PMid:24955428.

Pereira R, Sá R, Barros A, Sousa M. Major regulatory mechanisms involved in sperm motility. Asian J Androl. 2017;19(1):5-14. PMid:26680031.

Peris-Frau P, Soler AJ, Iniesta-Cuerda M, Martín-Maestro A, Sánchez-Ajofrín I, Medina-Chávez DA, Fernández-Santos MR, García-Álvarez O, Maroto-Morales A, Montoro V, Garde JJ. Sperm cryodamage in ruminants: understanding the molecular changes induced by the cryopreservation process to optimize sperm quality. Int J Mol Sci. 2020;21(8):2781. http://dx.doi.org/10.3390/ijms21082781. PMid:32316334.

Pintus E, Ros-Santaella JL. Impact of oxidative stress on male reproduction in domestic and wild animals. Antioxidants. 2021;10(7):1154. http://dx.doi.org/10.3390/antiox10071154. PMid:34356386.

Rahman SU, Huang Y, Zhu L, Feng S, Khan IM, Wu J, Li Y, Wang X. Therapeutic Role of green tea polyphenols in improving fertility: a review. Nutrients. 2018;10(7):834. http://dx.doi.org/10.3390/nu10070834. PMid:29954124.

Rakib-Uz-Zaman SM, Apu EH, Muntasir MN, Mowna SA, Khanom MG, Jahan SS, Akter N, Khan MAR, Shuborna NS, Shams SM, Khan K. Biosynthesis of silver nanoparticles from Cymbopogon citratus leaf extract and evaluation of their antimicrobial properties. Challenges. 2022;13(1):18. http://dx.doi.org/10.3390/challe13010018.

Ramachandran N, Yadav S, Sikarwar AKS, Saraswat S, Ranjan R, Jindal SK. Effect of equilibration periods on post-thaw semen quality of Jamunapari goats. Indian J Small Ruminants. 2015;21(2):234-7. http://dx.doi.org/10.5958/0973-9718.2015.00053.7.

Ratnawati D, Luthfi M. Comparative study of sperm motility analysis with CASA by using Leja and microscope slide. J Ilmu-Ilmu Peternakan. 2020;30(2):115-22. http://dx.doi.org/10.21776/ub.jiip.2020.030.02.03.

Ribas-Maynou J, Benet J. Single and double strand sperm DNA damage: different reproductive effects on male fertility. Genes. 2019;10(2):105. http://dx.doi.org/10.3390/genes10020105. PMid:30708937.

Roychoudhury S, Agarwal A, Virk G, Cho CL. Potential role of green tea catechins in the management of oxidative stress-associated infertility. Reprod Biomed Online. 2017;34(5):487-98. http://dx.doi.org/10.1016/j.rbmo.2017.02.006. PMid:28285951.

Saeki K, Hayakawa S, Nakano S, Ito S, Oishi Y, Suzuki Y, Isemura M. In vitro and in silico studies of the molecular interactions of Epigallocatechin-3-O-gallate (EGCG) with proteins that explain the health benefits of green tea. Molecules. 2018;23(6):1295. http://dx.doi.org/10.3390/molecules23061295. PMid:29843451.

Santoso A, Amrozi A, Purwantara B, Herdis H. Study of ovarian ultrasoundography of local goat synchronized with a single dose of Prostaglandin F2 Alfa Hormone (PGF2α). J Kedokt Hewan. 2014;8(1):38-42.

Sejian V, Silpa MV, Nair MRR, Devaraj C, Krishnan G, Bagath M, Chauhan SS, Suganthi RU, Fonseca VFC, König S, Gaughan JB, Dunshea FR, Bhatta R. Heat stress and goat welfare: adaptation and production considerations. Animals. 2021;11(4):1021. http://dx.doi.org/10.3390/ani11041021. PMid:33916619.

Selvam MKP, Agarwal A. A systematic review on sperm DNA fragmentation in male factor infertility: laboratory assessment. Arab J Urol. 2018;16(1):65-76. http://dx.doi.org/10.1016/j.aju.2017.12.001. PMid:29713537.

Sharma A, Sood P, Dogra P. Seminal plasma removal improves cryopreserved semen quality in Gaddi goats. Indian J Anim Reprod. 2018;39:25-8.

Sharma A, Sood P. Cryopreservation and fertility of frozen thawed Chegu goat semen. Indian J Anim Res. 2019;53(of):1414-9. http://dx.doi.org/10.18805/ijar.B-3696.

Sulistyo H, Kurniawan DW, Rujito L. Biochemical and histopathological effects of green tea nanoparticles in ironized mouse model. Res Pharm Sci. 2017;12(2):99-106. http://dx.doi.org/10.4103/1735-5362.202448. PMid:28515762.

Susilowati S, Mustofa I, Wurlina W, Hernawati T, Oktanella Y, Soeharsono S, Purwanto DA. Green tea extract in the extender improved the post-thawed semen quality and decreased amino acid mutation of Kacang buck sperm. Vet Sci. 2022;9(8):403. http://dx.doi.org/10.3390/vetsci9080403. PMid:36006318.

Susilowati S, Mustofa I, Wurlina W, Triana IN, Utama S, Rimayanti R. Effect of insulin-like growth factor-1 complex of Simmental bull seminal plasma on post-thawed Kacang buck semen fertility. Vet World. 2021a;14(8):2073-84. http://dx.doi.org/10.14202/vetworld.2021.2073-2084. PMid:34566323.

Susilowati S, Sardjito T, Mustofa I, Widodo OS, Kurnijasanti R. Effect of green tea extract in extender of Simmental bull semen on pregnancy rate of recipients. Anim Biosci. 2021b;34(2):198-204. http://dx.doi.org/10.5713/ajas.20.0025. PMid:32299169.

Susilowati S, Triana IN, Sardjito T, Suprayogi TW, Wurlina W, Mustofa I. Effect of Simmental bull seminal plasma protein in egg yolk-citrate extender on Kacang buck semen fertility. Cryobiology. 2020;97:20-7. http://dx.doi.org/10.1016/j.cryobiol.2020.10.013. PMid:33121627.

Zenteno ES, Rojano B, Betancur GR. Influence of thawing temperature on sperm motility, structure, and metabolism of frozen bovine semen. Cienc Rural. 2023;53(3):e20210731. http://dx.doi.org/10.1590/0103-8478cr20210731.

Zhang Z, Zhang X, Bi K, He Y, Yan W, Yang CS, Zhang J. Potential protective mechanisms of green tea polyphenol EGCG against COVID-19. Trends Food Sci Technol. 2021;114:11-24. http://dx.doi.org/10.1016/j.tifs.2021.05.023. PMid:34054222.

Zokti JA, Baharin BS, Mohammed AS, Abas F. Green tea leaves extract: microencapsulation, physicochemical and storage stability study. Molecules. 2016;21(8):940. http://dx.doi.org/10.3390/molecules21080940. PMid:27472310.
 


Submitted date:
02/10/2022

Accepted date:
12/22/2022

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