Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.1590/1984-3143-AR2022-0051
Animal Reproduction (AR)
ORIGINAL ARTICLE

Group-training of rams at puberty for artificial vagina-mediated semen collection and its influence on semen quality and sexual behavior

Majdi Ahmed Bahadi; Mohammed Abdo Al-Badwi; Emad Mohammed Samara; Khalid Ahmed Abdoun; Ibrahim Abdullah Alhidary; Ahmed Abraham Al-Haidary

Downloads: 1
Views: 577

Abstract

There is a paucity of information with respect to group-training for artificial vagina and its influence on semen characteristics and sexual behavior of young untrained rams. A total of 18 healthy Najdi rams (with an initial body weight of 40-45 Kg and 7-8 month-old) were consequently used herein to test the usefulness of group-training for artificial vagina-mediated semen collection during the breeding season. Rams were randomly segregated into three groups (n = 6 rams per protocol), and the whole experiment was lasted for 10 weeks. The 1st group was subjected to a training protocol where one untrained ram was placed for 20 min with a teaser ewe, while the 2nd group were subjected to a protocol where one untrained ram was placed for 20 min with one trained ram and a teaser ewe, whereas the 3rd group were subjected to a protocol where three untrained rams were placed for 20 min with one trained ram and a teaser ewe. The obtained results clearly (P < 0.05) showed that training young rams in group has increased their sperm concentration and sexual stimulation, shortened the period of their training time, and descriptively had a complete training efficiency. The sexual stimulation of young untrained rams was intensified by the competition between rams in the co-presence of a trained ram. Collectively, these data may suggest that group-training of rams at puberty is a better protocol for AV-mediated semen collection compared to individual training. Some shortcomings were noted herein, but research dealing with this subject may very well improve the reproductive performance of young untrained rams.

Keywords

artificial insemination, CASA, flehmen, libido, puberty

References

Almquist JO. Effects of sexual preparation on sperm output, semen characteristics and sexual activity of beef bulls with a comparison to dairy bulls. J Anim Sci. 1973;36(2):331-6. http://dx.doi.org/10.2527/jas1973.362331x. PMid:4687093.

Amann RP, Hammerstedt RH. In vitro evaluation of sperm quality: an opinion. J Androl. 1993;14(6):397-406. http://dx.doi.org/10.1002/j.1939-4640.1993.tb03247.x. PMid:8294222.

Amann RP. Can the fertility potential of a seminal sample be predicted accurately? J Androl. 1989;10(2):89-98. http://dx.doi.org/10.1002/j.1939-4640.1989.tb00066.x. PMid:2715106.

Ambrosi CP, Rubio N, Giménez G, Venturino A, Aisen EG, Armengol MF. Modeling of behavioral responses for successful selection of easy-to-train rams for semen collection with an artificial vagina. Anim Reprod Sci. 2018;193(1):90-7. http://dx.doi.org/10.1016/j.anireprosci.2018.04.003.

Broekhuijse ML, Šoštarić E, Feitsma H, Gadella BM. Application of computer-assisted semen analysis to explain variations in pig fertility. J Anim Sci. 2012;90(3):779-89. http://dx.doi.org/10.2527/jas.2011-4311. PMid:22064743.

Campanholi SP, Monteiro FM, Dias EA, Mercadante ME, Paz CC, Dell’Aqua JA Jr, Papa FO, Dell’Aqua CD, Vantini R, Garcia JM. Effect of seminal plasma removal before cryopreservation of bovine semen obtained by electroejaculation on semen quality and in vitro fertility. Theriogenology. 2017;89(1):114-21. http://dx.doi.org/10.1016/j.theriogenology.2016.10.008. PMid:28043341.

Carrillo E, Meza-Herrera CA, Olán-Sánchez A, Robles-Trillo PA, Leyva C, Luna-Orozco JR, Rodriguez-Martinez R, Veliz-Deras FG. The” female effect” positively affects the appetitive and consummatory sexual behaviour and testosterone concentrations of Alpine male goats under subtropical conditions. Czech J Anim Sci. 2014;59(7):337-43. http://dx.doi.org/10.17221/7532-CJAS.

Fahey AG, Duffy P, Fair S. Effect of exposing rams to a female stimulus before semen collection on ram libido and semen quality. J Anim Sci. 2012;90(10):3451-6. http://dx.doi.org/10.2527/jas.2011-4859. PMid:22665631.

Flores VA, Rosales RV, Trujillo AO. Training of rams for semen collection by artificial vagina, using as stimulus inanimate objects. Veterinaria . 2005;36(1):105-11.

Fonsêca VD, Saraiva EP, Pimenta EC Fo, Maior RJS Jr, Pereira WE, Silva AL, Silva Gomes DL, Andrade Moura JH. Sexual behavior and physical aspects of the semen of Moxotó breed goats at different ages. Semina: Ciênc Agrár. 2013;34(2):895-904. http://dx.doi.org/10.5433/1679-0359.2013v34n2p895.

Gerris J, Khan I. Correlation between in vitro fertilization and human sperm density and motility. J Androl. 1987;8(1):48-54. http://dx.doi.org/10.1002/j.1939-4640.1987.tb02419.x. PMid:3558110.

Gilmore A, Hitit M, Ugur MR, Dinh TTN, Tan W, Jousan D, Nicodemus M, Topper E, Kaya A, Memili E. Functional variables of bull sperm associated with cryotolerance. Kafkas Univ Vet Fak Derg. 2021;27(3). http://dx.doi.org/10.9775/kvfd.2021.25653.

Gonzalez R, Lévy F, Orgeur P, Poindron P, Signoret JP. Female effect in sheep. II. Role of volatile substances from the sexually receptive female; implication of the sense of smell. Reprod Nutr Dev. 1991;31(1):103-9. http://dx.doi.org/10.1051/rnd:19910110. PMid:2043257.

Hemsworth PH, Galloway DB. The effect of sexual stimulation on the sperm output of the domestic boar. Anim Reprod Sci. 1979;2(4):387-94. http://dx.doi.org/10.1016/0378-4320(79)90025-3.

Kathiravan P, Kalatharan J, Edwin MJ, Veerapandian C. Computer automated motion analysis of crossbred bull spermatozoa and its relationship with in vitro fertility in zona-free hamster oocytes. Anim Reprod Sci. 2008;104(1):9-17. http://dx.doi.org/10.1016/j.anireprosci.2007.01.002. PMid:17254723.

Krause W. Computer-assisted semen analysis systems: comparison with routine evaluation and prognostic value in male fertility and assisted reproduction. Hum Reprod. 1995;10(Suppl. 1):60-6. http://dx.doi.org/10.1093/humrep/10.suppl_1.60. PMid:8592042.

Maina D, Katz LS. Scent of a ewe: transmission of a social cue by conspecifics affects sexual performance in male sheep. Biol Reprod. 1999;60(6):1373-7. http://dx.doi.org/10.1095/biolreprod60.6.1373. PMid:10330095.

Malo AF, Garde JJ, Soler AJ, García AJ, Gomendio M, Roldan ER. Male fertility in natural populations of red deer is determined by sperm velocity and the proportion of normal spermatozoa. Biol Reprod. 2005;72(4):822-9. http://dx.doi.org/10.1095/biolreprod.104.036368. PMid:15576823.

McMurran S. Administering an analgesic during electroejaculation procedures in rams. In Pract. 2020;42(7):421-2. http://dx.doi.org/10.1136/inp.m2645.

Mulu M, Moges N, Adane M. Review on process, advantages and disadvantage of artificial insemination in cattle. Int J Vet Sci Anim Husb. 2018;3(6):8-13.

Nagy Á, Polichronopoulos T, Gáspárdy A, Solti L, Cseh S. Correlation between bull fertility and sperm cell velocity parameters generated by computer-assisted semen analysis. Acta Vet Hung. 2015;63(3):370-81. http://dx.doi.org/10.1556/004.2015.035. PMid:26551427.

Perkins A, Roselli CE. The ram as a model for behavioral neuroendocrinology. Horm Behav. 2007;52(1):70-7. http://dx.doi.org/10.1016/j.yhbeh.2007.03.016. PMid:17482616.

Prado V, Orihuela A, Lozano S, Pérez-León I. Effect on ejaculatory performance and semen parameters of sexually-satiated male goats (Capra hircus) after changing the stimulus female. Theriogenology. 2003;60(2):261-7. http://dx.doi.org/10.1016/S0093-691X(02)01373-0. PMid:12749939.

Prado V, Orihuela A, Lozano S, Perez-Leon I. Management of the female stimulus during semen collection and its association with libido re-establishment and semen characteristics of goats. J Anim Sci. 2002;80(6):1520-3. http://dx.doi.org/10.2527/2002.8061520x. PMid:12078732.

Preston BT, Stevenson IR, Lincoln GA, Monfort SL, Pilkington JG, Wilson K. Testes size, testosterone production and reproductive behaviour in a natural mammalian mating system. J Anim Ecol. 2012;81(1):296-305. http://dx.doi.org/10.1111/j.1365-2656.2011.01907.x. PMid:21958300.

Rout PK, Kaushik R, Ramachandran N, Jindal SK. Identification of heat stress-susceptible and-tolerant phenotypes in goats in semiarid tropics. Anim Prod Sci. 2018;58(7):1349-57. http://dx.doi.org/10.1071/AN15818.

Santos SG, Saraiva EP, Pimenta EC Fo, Santos LD, Fonsêca VD, Veríssimo TN, Almeida ME, Pinheiro AD. Seasonal and circadian variation of the sexual behavior of Morada Nova rams in tropical environment. Rev Bras Zootec. 2015;44(1):8-14. http://dx.doi.org/10.1590/S1806-92902015000100002.

Silvestre MA, Salvador I, Sanchez JP, Gómez EA. Effect of changing female stimulus on intensive semen collection in young Murciano-Granadina male goats. J Anim Sci. 2004;82(6):1641-5. http://dx.doi.org/10.2527/2004.8261641x. PMid:15216989.

Stafford KJ. Electroejaculation: a welfare issue. Surveillance. 1995;22:15-7.
 


Submitted date:
05/11/2022

Accepted date:
03/08/2023

643ed18da953956b734e5f44 animreprod Articles
Links & Downloads

Anim Reprod

Share this page
Page Sections