Animal Reproduction (AR)
Animal Reproduction (AR)

Does combining estradiol cypionate and GnRH for ovulation induction in recipient cows increase pregnancy rate after timed embryo transfer?

Wagner Marques Lima; Fabiane Pereira de Moraes; Rogério Ferreira; Rafael Gianella Mondadori; Arnaldo Diniz Vieira; Nathália Wacholz Knabah; Danylo Cintra Medeiros Lima; Monique Tomazele Rovani; Luiz Francisco Machado Pfeifer; Paulo Bayard Dias Gonçalves; Bernardo Garziera Gasperin

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Estradiol cypionate (EC) or GnRH have been widely used for ovulation induction in timed embryo transfer (TET). EC administration increases the proportion of cows that show estrus, whereas GnRH promotes more synchronized ovulations. The aim of the present study was to evaluate the potential beneficial effects of combining EC and GnRH in TET. In experiment 1, no difference was observed on serum progesterone concentrations on Day 6 and 13 after GnRH treatment between GnRH and EC+GnRH groups. In experiment 2, pregnancy per embryo transfer (P/ET) did not differ (p = 0.69) between GnRH (62.8%) and EC+GnRH (58.7%) groups. In conclusion, combining EC and GnRH for ovulation induction does not increase progesterone secretion and pregnancy rate after TET in cattle.


gestation, luteal function, progesterone, synchronization


Barbosa IP, Cestaro JP, Silva SA, Noleto GS, Gonçalves RL, Silva GM, Paes FH, Gasperin BG, Rovani MT, Pfeifer LFM. GnRH34: an alternative for increasing pregnancy in timed AI beef cows. Theriogenology. 2022;179:1-6. PMid:34823056.

Bó GA, Cedeño A. Expression of estrus as a relevant factor in fixed-time embryo transfer programs using estradiol/progesterone-based protocols in cattle. Anim Reprod. 2018;15(3):224-30. PMid:34178145.

Consentini CEC, Wiltbank MC, Sartori R. Factors that optimize reproductive efficiency in dairy herds with an emphasis on timed artificial insemination programs. Animals (Basel). 2021;11(2):301. PMid:33503935.

Frade MC, Frade C, Cordeiro MB, Sá MF Fo, Mesquita FS, Nogueira GP, Binelli M, Membrive CM. Manifestation of estrous behavior and subsequent progesterone concentration at timed-embryo transfer in cattle are positively associated with pregnancy success of recipients. Anim Reprod Sci. 2014;151(3-4):85-90. PMid:25449549.

Hasler JF. Forty years of embryo transfer in cattle: a review focusing on the journal Theriogenology, the growth of the industry in North America, and personal reminisces. Theriogenology. 2014;81(1):152-69. PMid:24274419.

Pérez-Mora A, Segura-Correa JC, Peralta-Torres JA. Factors associated with pregnancy rate in fixed-time embryo transfer in cattle under humid-tropical conditions of México. Anim Reprod. 2020;17(2):e20200007. PMid:32714459.

Pfeifer LFM, Moreira EM, da Silva GM, de Souza VL, Nunes VRR, Andrade JS, Neves PMA, Ferreira R. Effect of estradiol cypionate on estrus expression and pregnancy in timed artificially inseminated beef cows. Livest Sci. 2020;231:103886.

Pfeifer LFM, Rodrigues WB, Nogueira E. Relationship between body condition score index and fertility in beef cows subjected to timed artificial insemination. Livest Sci. 2021;248:104482.

Sá MF Fo, Gonella-Diaza AM, Sponchiado M, Mendanha MF, Pugliesi G, Ramos RDS, Andrade SCS, Gasparin G, Coutinho LL, Goissis MD, Mesquita FS, Baruselli PS, Binelli M. Impact of hormonal modulation at proestrus on ovarian responses and uterine gene expression of suckled anestrous beef cows. J Anim Sci Biotechnol. 2017;8(1):79. PMid:29118976.

Souza AH, Viechnieski S, Lima FA, Silva FF, Araújo R, Bó GA, Wiltbank MC, Baruselli PS. Effects of equine chorionic gonadotropin and type of ovulatory stimulus in a timed-AI protocol on reproductive responses in dairy cows. Theriogenology. 2009;72(1):10-21. PMid:19269685.

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