Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.1590/1984-3143-AR2022-0100
Animal Reproduction (AR)
SHORT COMMUNICATION

Sperm cryopreservation in Windsnyer boars; principles, technique, and updated outcomes

Mamonene Angelinah Thema; Masindi Lottus Mphaphathi; Mahlatsana Ramaesela Ledwaba; Tshimangadzo Lucky Nedambale

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Abstract

Abstract: The domestic pig breeds are in danger of extinction whereas the erosion of their gene pool is a serious concern because they significantly contribute to the rich biodiversity. Overall aim of this study was to determine the protocol for preserving the semen of the Windsnyer boars for conservation. A total of 18 ejaculates (6 replications/boar) were collected from three Windsnyer boars of proven fertility with the use of hand-gloved approach method, twice per week. Boars semen were pooled and extended with Beltsville Thawing Solution [(BTS) IMV Technologies, France], held at 18°C for 3 hours and centrifuged. The sperm pellet was re-suspended with Fraction A (20% egg yolk + BTS) and cooled at 5°C for 1 hour. Following cooling, semen was divided and diluted into different cryoprotectants (ethylene glycol, glycerol, propanediol, ethylene glycol + glycerol + propanediol) at equal contribution to make the total concentrations [4, 8, 12 and 16% and the 0% (control; without cryoprotectant)] and loaded into 0.25 mL straws. Two cryopreservation methods (liquid nitrogen vapour and controlled rated) were used to cryopreserve the semen straws. Semen straws were thawed at different temperatures (5, 18, 37 and 40°C) and evaluated for sperm motility, viability, and morphology traits. Post-thawed sperm total motility (36.0±5.3) and live normal sperm (49.5±8.3) percentages were recorded to be higher in the treatment supplemented with 16% glycerol (P<0.05). The highest sperm total motility percentage was recorded at 40°C (26.8±3.2) thawing temperature for liquid nitrogen vapour treatment (P<0.05). In conclusion, 16% glycerol was found to be the suitable cryoprotectant concentration for semen cryopreserved with liquid nitrogen vapour method and thawed at 40°C.

Keywords

glycerol, propanediol, ethylene glycol, cryopreservation, Windsnyer boars

References

Almubarak AM, Kim W, Abdelbagi NH, Balla SE, Yu IJ, Jeon Y. Washing solution and centrifugation affect the kinematics of cryopreserved boar semen. J Anim Reprod Biotechnol. 2021;36(2):65-75. http://dx.doi.org/10.12750/JARB.36.2.69.

Bovula N, Ncobela CN, Pilane CM, Nedambale TL, Chimonyo M. Growth performance and fertility of Windsnyer boars supplemented with α-tocopherol. Trop Anim Health Prod. 2021;53(1):161. http://dx.doi.org/10.1007/s11250-021-02610-9. PMid:33580340.

Bustani GS, Baiee FH. Semen extenders: an evaluative overview of preservative mechanisms of semen and semen extenders. Vet World. 2021;14(5):1220-33. http://dx.doi.org/10.14202/vetworld.2021.1220-1233. PMid:34220124.

Caamaño JN, Tamargo C, Parrilla I, Martínez-Pastor F, Padilla L, Salman A, Fueyo C, Fernández Á, Merino MJ, Iglesias T, Hidalgo CO. Post-thawed sperm quality and functionality in the Autochthonous pig breed Gochu Asturcelta. Animals. 2021;11(7):1885. http://dx.doi.org/10.3390/ani11071885. PMid:34202862.

Hlongwane NL, Hadebe K, Soma P, Dzomba EF, Muchadeyi FC. Genome-wide assessment of genetic variation and population distinctiveness of the pig family in South Africa. Front Genet. 2020;11:344. http://dx.doi.org/10.3389/fgene.2020.00344. PMid:32457791.

Holt WV. Basic aspects of frozen storage of semen. Anim Reprod Sci. 2000;62(1-3):3-22. http://dx.doi.org/10.1016/S0378-4320(00)00152-4. PMid:10924818.

Jovičić M, Chmelίková E, Sedmίková M. Cryopreservation of boar semen. Czech J Anim Sci. 2020;65(4):115-23. http://dx.doi.org/10.17221/47/2020-CJAS.

Kaeoket K, Tantiparinyakul K, Kladkaew W, Chanapiwat P, Techkumphu M. Effect of different antioxidants on quality of cryopreserved boar semen in different breeds. Thai J Agric Sci. 2008;41(1-2):1-9.

Kaeoket K. Cryopreservation of boar spermatozoa: an important role of antioxidants. In: Katkov II, editor. Current frontiers in cryopreservation. Rijeka: InTech; 2012. p. 139-64. http://dx.doi.org/10.5772/32868.

Madzimure J, Chimonyo M, Zander KK, Dzama K. Potential for using indigenous pigs in subsistence-oriented and market-oriented small-scale farming systems of Southern Africa. Trop Anim Health Prod. 2012;45(1):135-42. http://dx.doi.org/10.1007/s11250-012-0184-3. PMid:22639035.

Mapeka MH, Lehloenya KC, Nedambale TL, Sutherland B. Effect of cryoprotectant on the cryopreservation of South African Kolbroek pig semen. S Afr J Anim Sci. 2009;39(1):198-201.

Marsoner T, Vigl LE, Manck F, Jaritz G, Tappeiner U, Tasser E. Indigenous livestock breeds as indicators for cultural ecosystem services: a spatial analysis within the Alpine spae. Ecol Indic. 2018;94:55-63. http://dx.doi.org/10.1016/j.ecolind.2017.06.046.

Martinez EA, Vazquez JM, Roca J, Lucas X, Gil MA, Parrilla I, Vazquez JL, Day BN. Successful non-surgical deep intrauterine insemination with small numbers of spermatozoa in sows. Reproduction. 2001;122(2):289-96. http://dx.doi.org/10.1530/rep.0.1220289. PMid:11467980.

Monteiro RA, Cunha RM, Guerra MMP, Almeida VM, Pena-Alfaro CE, Silva SV. Use of equine sperm cryopreservation techniques as a conservation method of donkey germplasm. J Equine Vet Sci. 2022;111:103888. http://dx.doi.org/10.1016/j.jevs.2022.103888. PMid:35101525.

Morris GJ, Faszer K, Green JE, Draper D, Grout BWW, Fonseca F. Rapidly cooled horse sperm: loss of viability is due to osmotic imbalance during thawing, not intracellular ice formation. Theriogenology. 2007;68(5):804-12. http://dx.doi.org/10.1016/j.theriogenology.2007.06.009. PMid:17645937.

Parrilla I, Martinez EA, Gil MA, Cuello C, Roca J, Rodriguez-Martinez H, Martinez CA. Boar seminal plasma: current insights on its potential role for assisted reproductive technologies in swine. Anim Reprod. 2020;17(3):e20200022. http://dx.doi.org/10.1590/1984-3143-ar2020-0022. PMid:33029213.

Roberts KS, Lamberson WR. Relationships among and variation within rare breeds of swine. J Anim Sci. 2015;93(8):3810-3. http://dx.doi.org/10.2527/jas.2015-9001. PMid:26440160.

Roca J, Carvajal G, Lucas X, Vazquez JM, Martinez EA. Fertility of weaned sows after deep intrauterine insemination with a reduced number of frozen-thawed spermatozoa. Theriogenology. 2003;60(1):77-87. http://dx.doi.org/10.1016/S0093-691X(02)01330-4. PMid:12620582.

Salih SA, Daghigh-Kia H, Mehdipour M, Najafi A. Does ergothioneine and thawing temperatures improve rooster semen post-thawed quality? Poult Sci. 2021;100(10):101405. http://dx.doi.org/10.1016/j.psj.2021.101405. PMid:34464932.

Sancho S, Casas I, Ekwall H, Saravia F, Rodriguez-Martinez H, Rodriguez-Gil JE, Flores E, Pinart E, Briz M, Garcia-Gil N, Bassols J, Pruneda A, Bussalleu E, Yeste M, Bonet S. Effects of cryopreservation on semen quality and the expression of sperm membrane hexose transporters in the spermatozoa of Iberian pigs. Reproduction. 2007;134(1):111-21. http://dx.doi.org/10.1530/REP-07-0118. PMid:17641093.

Seshoka MM, Mphaphathi ML, Nedambale TL. Comparison of four different permitting and combination of two best cryoprotectants on freezing Nguni sperm evaluated with the aid of computer aided sperm analysis. Cryobiology. 2016;72(3):232-8. http://dx.doi.org/10.1016/j.cryobiol.2016.04.001. PMid:27059770.

Silva CG, Cunha ER, Blume GR, Malaquias JV, Bao S, Martins CF. Cryopreservation of boar sperm comparing different cryoprotectants associated in media based on powdered coconut water, lactose, and trehalose. Cryobiology. 2015;70(2):90-4. http://dx.doi.org/10.1016/j.cryobiol.2015.01.001. PMid:25595634.

Thema MA, Mphaphathi ML, Ledwaba MR, Nedambale TL. Investigation of the efficacy of different semen extenders and in vitro storage period on Windsnyer boar sperm quality equilibrated at 18°C. Reprod Domest Anim. 2022;57(12):1644-8. http://dx.doi.org/10.1111/rda.14256. PMid:36086994.

Tomás-Almenar C, Mercado E. Optimization of the thawing protocol for Iberian boar sperm. Animals. 2022;12(19):2600. http://dx.doi.org/10.3390/ani12192600. PMid:36230350.

Vishwanath R, Shannon P. Storage of bovine semen in the liquid and frozen state. Anim Reprod Sci. 2000;62(1-3):23-53. http://dx.doi.org/10.1016/S0378-4320(00)00153-6. PMid:10924819.

Watson PF. The causes of reduced fertility with cryopreserved semen. Anim Reprod Sci. 2000;60-61:481-92. http://dx.doi.org/10.1016/S0378-4320(00)00099-3. PMid:10844218.

Wu TW, Cheng FP, Chen IH, Yang CH, Tsai MY, Chang MH, Wang JH, Wu JT. The combinatorial effect of different Equex STM paste concentrations, cryoprotectants and the straw-freezing methods on the post-thaw boar semen quality. Reprod Domest Anim. 2013;48(1):53-8. http://dx.doi.org/10.1111/j.1439-0531.2012.02022.x. PMid:22612249.

Yánez-Ortiz I, Catalán J, Rodríguez-Gil JE, Miró J, Yeste M. Advances in sperm cryopreservation in farm animals: cattle, horse, pig and sheep. Anim Reprod Sci. 2022;246:106904. http://dx.doi.org/10.1016/j.anireprosci.2021.106904. PMid:34887155.

Yeste M, Rodríguez-Gil JE, Bonet S. Artificial insemination with frozen-thawed boar sperm. Mol Reprod Dev. 2017;84(9):802-13. http://dx.doi.org/10.1002/mrd.22840. PMid:28608609.

Zong Y, Sun Y, Li Y, Mehaisen GM, Yuan J, Ma H, Ni A, Wang Y, Hamad SK, Elomda AM, Abbas AO, Chen J. Effect of glycerol concentration, glycerol removal method, and straw type on the quality and fertility of frozen chicken semen. Poult Sci. 2022;101(6):101840. http://dx.doi.org/10.1016/j.psj.2022.101840. PMid:35413595.
 


Submitted date:
10/17/2022

Accepted date:
05/29/2023

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