Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.1590/1984-3143-AR2022-0105
Animal Reproduction (AR)
ORIGINAL ARTICLE

Insulin-like growth factor-1 improves in vitro meiotic resumption of dromedary camel (Camelus Dromedarius) oocytes

Abdulrahman Khalid Alhaider

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Abstract

Despite relatively high maturation rate of in vitro matured oocytes in the dromedary camel, however, blastocyst production is very low after in vitro fertilization (IVF). Herein, the influences of oocyte collection method (follicular aspiration vs slicing; Experiment I), the addition of Insulin-like growth factor I (IGF-I) to the maturation medium (Experiment II) on in vitro maturation (IVM) of oocyte were investigated. Although the nuclear maturation did not differ regardless of collecting method, follicular aspiration led to lower degeneration rates than those in controls (P < 0.05). The percentages of oocytes at MII were greater in the presence of IGF-1 than in its absence (71.9% vs 48.4%, respectively, P<0.05). Additionally, the percentages of degenerated oocytes were higher in the control group compared to oocytes cultured in the presence of IGF-I (23.6% vs 10.4%, respectively, P<0.05). IGF-I treatment improved the quality of MII matured oocytes as evidenced by the decrease of cathepsin B (CTSB) activity, a marker of poor quality oocytes, when compared to control ones (P < 0.05). In conclusion, follicular aspiration decreased the degeneration rate; however, it had no effect on completion of maturation. IGF-I enhanced the IVM of oocyte and decreased degeneration rate.

Keywords

camel, cathepsin B, insulin-like growth factor I, IVM, oocytes

References

Abdel-Ghani MA, Yanagawa Y, Balboula AZ, Sakaguchi K, Kanno C, Katagiri S, Takahashi M, Nagano M. Astaxanthin improves the developmental competence of in vitro grown oocytes and modifies the steroidogenesis of granulosa cells derived from bovine early antral follicles. Reprod Fertil Dev. 2019;31(2):272-81. http://dx.doi.org/10.1071/RD17527. PMid:30071922.

Abdoon ASS, Kandil OM, Berisha B, Kliem H, Schams D. Morphology of dromedary camel oocytes and their ability to spontaneous and chemical parthenogenetic activation. Reprod Domest Anim. 2007;42(1):88-93. http://dx.doi.org/10.1111/j.1439-0531.2006.00737.x. PMid:17214780.

Abdoon ASS. Factors affecting follicular population, oocyte yield and quality in camels (Camelus dromedarius) ovary with special reference to maturation time in vitro. Anim Reprod Sci. 2001;66(1-2):71-9. http://dx.doi.org/10.1016/S0378-4320(01)00078-1. PMid:11343843.

Adashi EY, Resnick CE, D’Ercole AJ, Svoboda ME, Van Wyk JJ. Insulin-like growth factors as intraovarian regulators of granulosa cell growth and function. Endocr Rev. 1985;6(3):400-20. http://dx.doi.org/10.1210/edrv-6-3-400. PMid:2992919.

Alhaider AK, Watson PF. The effects of hCG and growth factors on in vitro nuclear maturation of dog oocytes obtained during anoestrus. Reprod Fertil Dev. 2009;21(4):538-48. http://dx.doi.org/10.1071/RD08167. PMid:19383260.

Arat S, Caputcu AT, Cevik M, Akkoc T, Cetinkaya G, Bagis H. Effect of growth factors on oocyte maturation and allocations of inner cell mass and trophectoderm cells of cloned bovine embryos. Zygote. 2016;24(4):554-62. http://dx.doi.org/10.1017/S0967199415000519. PMid:26444069.

Araujo MS, Guastali MD, Paulini F, Silva AN, Tsunemi MH, Fontes PK, Castilho ACS, Landim-Alvarenga FCL. Molecular and cellular effects of insulin-like growth factor-1 and LongR3-IGF-1 on in vitro maturation of bovine oocytes: comparative study. Growth Horm IGF Res. 2020;55:101357. http://dx.doi.org/10.1016/j.ghir.2020.101357. PMid:33038561.

Arlotto TM, Leibfried-Rutledge ML, First NL. Size distribution and meiotic competence of bovine primary oocytes from two locations in the ovary. Theriogenology. 1990;33(1):188. http://dx.doi.org/10.1016/0093-691X(90)90612-W.

Balboula AZ, Yamanaka K, Sakatani M, Hegab A, Zaabel SM, Takahashi M. Intracellular cathepsin B activity is inversely correlated with the quality and developmental competence of bovine preimplantation embryos. Mol Reprod Dev. 2010;77(12):1031-9. http://dx.doi.org/10.1002/mrd.21250. PMid:21104746.

Balboula AZ, Yamanaka K, Sakatani M, Kawahara M, Hegab AO, Zaabel SM, Takahashi M. Cathepsin B activity has a crucial role in the developmental competence of bovine cumulus-oocyte complexes exposed to heat shock during in vitro maturation. Reproduction. 2013;146(4):407-17. http://dx.doi.org/10.1530/REP-13-0179.

Demeestere I, Gervy C, Centner J, Devreker F, Englert Y, Delbaere A. Effect of insulin-like growth factor-I during preantral follicular culture on steroidogenesis, in vitro oocyte maturation, and embryo development in mice. Biol Reprod. 2004;70(6):1664-9. http://dx.doi.org/10.1095/biolreprod.103.023317. PMid:14960488.

El-Bahr SM, Ghoneim IM, Waheed MM. Biochemical and hormonal analysis of follicular fluid and serum of female dromedary camels (Camelus dromedarius) with different sized ovarian follicles. Anim Reprod Sci. 2015;159:98-103. http://dx.doi.org/10.1016/j.anireprosci.2015.06.002. PMid:26077770.

El-Deeb W, Abdelghani MA, Alhaider A, Fayez M. Oxidative stress, ceruloplasmin and neopterin biomarkers in dromedary camels with clinical endometritis. Anim Reprod. 2022;19(3):e20220035. http://dx.doi.org/10.1590/1984-3143-ar2022-0035. PMid:36156882.

Fathi M, Seida AA, Sobhy RR, Darwish GM, Badr MR, Moawad AR. Caffeine supplementation during IVM improves frequencies of nuclear maturation and preimplantation development of dromedary camel oocytes following IVF. Theriogenology. 2014;81(9):1286-92. http://dx.doi.org/10.1016/j.theriogenology.2014.02.010. PMid:24630529.

Gabr SA, Samiah SM, Nagy WM. Effect of leptin supplementation in maturation medium on in vitro nuclear maturation and fertilization of camel oocytes (Camelus dromedarius). As. J Anim Vet Adv. 2014;9(11):713-20. http://dx.doi.org/10.3923/ajava.2014.713.720.

Giudice LC. Insulin-like growth factors and ovarian follicular development. Endocr Rev. 1992;13(4):641-69. PMid:1281087.

Gómez E, Tarín JJ, Pellicer A. Oocyte maturation in humans: the role of gonadotropins and growth factors. Fertil Steril. 1993;60(1):40-6. http://dx.doi.org/10.1016/S0015-0282(16)56033-6. PMid:8513957.

Herrler A, Krusche CA, Beier HM. Insulin and insulin-like growth factor-I promote rabbit blastocyst development and prevent apoptosis. Biol Reprod. 1998;59(6):1302-10. http://dx.doi.org/10.1095/biolreprod59.6.1302. PMid:9828171.

Iwasaki S, Kono T, Nakahara T, Shioya Y, Fukushima M, Hanada A. New method for the recovery of oocytes from bovine ovarian tissue in relation to in vitro maturation and fertilization. J J Anim Reprod. 1987;33(4):188-92. http://dx.doi.org/10.1262/jrd1977.33.188.

Kafi M, Mesbah SF, Davoodian N, Kadivar A. Fine structures of the oocyte in relation to serum, follicular fluid steroid hormones and IGF-I in the ovulatory-sized follicles in one-humped camel (Camelus dromedarius). Avicenna J Med Biotechnol. 2014;6(1):57-61. PMid:24551433.

Kamada S, Kubota T, Taguchi M, Ho WR, Sakamoto S, Aso T. Effects of insulin-like growth factor-II on proliferation and differentiation of ovarian granulosa cells. Horm Res. 1992;37(4-5):141-9. http://dx.doi.org/10.1159/000182299. PMid:1337056.

Khatir H, Anouassi A, Tibary A. Effect of follicular size on in vitro developmental competence of oocytes and viability of embryos after transfer in the dromedary (Camelus dromedarius). Anim Reprod Sci. 2007;99(3-4):413-20. http://dx.doi.org/10.1016/j.anireprosci.2006.06.015. PMid:16889909.

Khatir H, Anouassi A, Tibary A. In vitro and in vivo developmental competence of dromedary (Camelus dromedarius) embryos produced in vitro using two culture systems (mKSOMaa and Oviductal Cells). Reprod Domest Anim. 2005;40(3):245-9. http://dx.doi.org/10.1111/j.1439-0531.2005.00587.x. PMid:15943699.

Khatir H, Anouassi A. The first dromedary (Camelus dromedarius) offspring obtained from in vitro matured, in vitro fertilized and in vitro cultured abattoir-derived oocytes. Theriogenology. 2006;65(9):1727-36. http://dx.doi.org/10.1016/j.theriogenology.2005.09.029. PMid:16263162.

Kumar A, Solanki VS, Jindal SK, Tripathi VN, Jain GC. Oocyte retrieval and histological studies of follicular population in buffalo ovaries. Anim Reprod Sci. 1997;47(3):189-95. http://dx.doi.org/10.1016/S0378-4320(96)01588-6. PMid:9329860.

Kushnir VA, Barad DH, Albertini DF, Darmon SK, Gleicher N. Systematic review of worldwide trends in assisted reproductive technology. Reprod Biol Endocrinol. 2017;15(1):6. http://dx.doi.org/10.1186/s12958-016-0225-2. PMid:28069012.

Leisinger CA, Coffman EA, Silva MAC, Forshey BS, Pinto CRF. Factors affecting in vitro maturation of alpaca (Lama paco) oocytes. Anim Reprod Sci. 2014;150(1-2):70-5. http://dx.doi.org/10.1016/j.anireprosci.2014.08.011. PMid:25261077.

Lorenzo PL, Illera MJ, Illera JC, Illera M. Enhancement of cumulus expansion and nuclear maturation during bovine oocyte maturation in vitro by the addition of epidermal growth factor and insulin-like growth factor I. J Reprod Fertil. 1994;101(3):697-701. http://dx.doi.org/10.1530/jrf.0.1010697. PMid:7966028.

Lorenzo PL, Rebollar PG, Illera MJ, Illera JC, Illera M, Alvarino JM. Stimulatory effect of insulin-like growth factor I and epidermal growth factor on the maturation of rabbit oocytes in vitro. J Reprod Fertil. 1996;107(1):109-17. http://dx.doi.org/10.1530/jrf.0.1070109. PMid:8699422.

Martino A, Palomo MJ, Mogas T, Paramio MT. Influence of the collection technique of prepubertal goat oocytes on in vitro maturation and fertilization. Theriogenology. 1994;42(5):859-73. http://dx.doi.org/10.1016/0093-691X(94)90454-Q. PMid:16727591.

Oberlender G, Murgas LDS, Zangeronimo MG, Silva AC, Menezes TA, Pontelo TP, Vieira LA. Role of insulin-like growth factor-I and follicular fluid from ovarian follicles with different diameters on porcine oocyte maturation and fertilization in vitro. Theriogenology. 2013;80(4):319-27. http://dx.doi.org/10.1016/j.theriogenology.2013.04.018. PMid:23683690.

Sato A, Sarentonglaga B, Ogata K, Yamaguchi M, Hara A, Atchalalt K, Sugane N, Fukumori R, Nagao Y. Effects of insulin-like growth factor-1 on the in vitro maturation of canine oocytes. J Reprod Dev. 2018;64(1):83-8. http://dx.doi.org/10.1262/jrd.2017-145. PMid:29212962.

Szamatowicz M. Assisted reproductive technology in reproductive medicine - possibilities and limitations. Ginekol Pol. 2016;87(12):820-3. http://dx.doi.org/10.5603/GP.2016.0095. PMid:28098933.

Toori MA, Mosavi E, Nikseresht M, Barmak MJ, Mahmoudi R. Influence of insulin-like growth factor-I on maturation and fertilization rate of immature oocyte and embryo development in NMRI mouse with TCM199 and alpha-MEM medium. J Clin Diagn Res. 2014;8(12):AC05-08. http://dx.doi.org/10.7860/JCDR/2014/9129.5242. PMid:25653928.

Wani NA, Nowshari MA. Kinetics of nuclear maturation and effect of holding ovaries at room temperature on in vitro maturation of camel (Camelus dromedarius) oocytes. Theriogenology. 2005;64(1):75-85. http://dx.doi.org/10.1016/j.theriogenology.2004.11.009. PMid:15935844.

Wani NA, Wernery U. In vitro maturation of dromedary (Camelus dromedarius) oocytes: effect of different protein supplementations and epidermal growth factor. Reprod Domest Anim. 2010;45(5):e189-93. PMid:20088850.

Wasielak M, Bogacki M. Apoptosis inhibition by insulin-like growth factor (IGF)-I during in vitro maturation of bovine oocytes. J Reprod Dev. 2007;53(2):419-26. http://dx.doi.org/10.1262/jrd.18076. PMid:17179657.
 


Submitted date:
11/25/2022

Accepted date:
05/08/2023

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