Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.1590/1984-3143-AR2023-0005
Animal Reproduction (AR)
ORIGINAL ARTICLE

Effects of salinity on pre- and post-fertilization developmental events in the clam Anomalocardia flexuosa (Linnaeus, 1767)

Rodolf Gabriel Prazeres Silva Lopes; Ana Paula Rego; Sabrina Melo de Jesus Gomes; Thayane Ramos; Ícaro Gomes Antonio; Maria Raquel Moura Coimbra

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Abstract

The knowledge about the effect of salinity on the physiological mechanism of bivalve reproduction is fundamental to improve production strategies in hatcheries. The present work evaluated the influence of different salinity concentrations (15, 20, 25, 30, 35 and 40 g⋅L−1) on pre- and post-fertilization development processes in the clam, Anomalocardia flexuosa, oocytes obtained by stripping. Salinity directly interfered with the germinal vesicle breakdown (GVBD) rate and in the cellular stability of unfertilized oocytes. Salinity concentrations between 30 and 35 g⋅L−1 provided better percentages of stable GVBD within 120 min, and incubation of oocytes in the salinity range of 30-35 g⋅L−1 for a time interval of 80-120 min provided > 80% GVBD. In the post-fertilization analysis, salinity affected the rate of the extrusion of the first and second polar bodies (PB1 and PB2). The release of 50% of the PBs was faster at a salinity of 35 g⋅L−1, with an estimated time of 10 min for PB1 and 30 min for PB2. Thus, chromosome manipulation methodologies aiming triploids should be applied at 35 g⋅L−1 salinity, with application of post-fertilization shock before 10 min for PB1 retention or before 30 min for PB2 retention.

Keywords

tropical bivalve reproduction, stripping, germinal vesicle breakdown, polar body

References

Abbott RT. American seashells. 2nd ed. New York: Van Nostrand Reinhold Company; 2011. https://doi.org/10.5962/bhl.title.7471.

Allen RD. Fertilization and artificial activation in the egg of the surf-clam, Spisula solidissima. Biol Bull. 1953;105(2):213-39. http://dx.doi.org/10.2307/1538639.

Allen SK Jr, Bushek D. Large-scale production of triploid oysters, Crassostrea virginica (Gmelin), using “stripped” gametes. Aquaculture. 1992;103(3-4):241-51. http://dx.doi.org/10.1016/0044-8486(92)90170-P.

Brasil. Lei nº 11.794, de 8 de outubro de 2008. Regulamenta o inciso VII do § 1º do art. 225 da Constituição Federal, estabelecendo procedimentos para o uso científico de animais; revoga a Lei nº 6.638, de 8 de maio de 1979; e dá outras providências. Diário Oficial da União; Brasil; 9 October 2008.

Camacho EG, Dominguez GR, Armenta OOZ, Haws M, Supan J, Bajo LJA, Llamas GH, Valenzuela JEV, Juárez RNP. Developing hatchery methods for the mangrove oyster, Crassostrea corteziensis for the Pacific Coast of Mexico. Culiacán: Autonomous University of Sinaloa; 2011. Final reports: investigations 2009–2011.

Colas P, Dubé F. Meiotic maturation in mollusc oocytes. Semin Cell Dev Biol. 1998;9(5):539-48. http://dx.doi.org/10.1006/scdb.1998.0248. PMid:9835642.

Dégremont L, Garcia C, Frank-Lawale A, Allen SK. Triploid oysters in the Chesapeake Bay: comparison of diploid and triploid Crassostrea virginica. J Shellfish Res. 2012;31(1):21-31. http://dx.doi.org/10.2983/035.031.0103.

Dheilly NM, Jouaux A, Boudry P, Favrel P, Lelong C. Transcriptomic profiling of gametogenesis in triploid pacific oysters Crassostrea gigas: towards an understanding of partial sterility associated with triploidy. PLoS One. 2014;9(11):e112094. http://dx.doi.org/10.1371/journal.pone.0112094. PMid:25375782.

Downing SL, Allen SK Jr. Induced triploidy in the Pacific oyster, Crassostrea gigas: optimal treatments with cytochalasin B depend on temperature. Aquaculture. 1987;61(1):1-15. http://dx.doi.org/10.1016/0044-8486(87)90332-2.

Eudeline B, Allen SK Jr, Guo X. Delayed meiosis and polar body release in eggs of triploid Pacific oysters, Crassostrea gigas, in relation to tetraploid production. J Exp Mar Biol Ecol. 2000;248(2):151-61. http://dx.doi.org/10.1016/S0022-0981(00)00158-1. PMid:10771299.

Guimarães IM, Antonio ÍG, Peixoto S, Olivera A. Salinity influence on the survival of mangrove oyster, Crassostrea rhizophorae. Arq Ciênc Mar. 2008;41(1):118-22.

Guo X, Allen SK Jr. Sex determination and polyploid gigantism in the dwarf surfclam (Mulinia lateralis say). Genetics. 1994;138(4):1199-206. http://dx.doi.org/10.1093/genetics/138.4.1199. PMid:7896101.

Guo X, DeBrosse GA, Allen SK Jr. All-triploid Pacific oysters (Crassostrea gigas Thunberg) produced by mating tetraploids and diploids. Aquaculture. 1996;142(3-4):149-61. http://dx.doi.org/10.1016/0044-8486(95)01243-5.

Guo X, Wang Y, Xu Z, Yang H. Chromosome set manipulation in shellfish. In: Burnell G, Allan G, editors. New technologies in aquaculture: improving production efficiency, quality and environmental management. Cambridge: Woodhead Publishing; 2009. p. 165–94. http://dx.doi.org/10.1533/9781845696474.1.165.

Kim WJ, Dammannagoda ST, Jung H, Baek IS, Yoon HS, Choi SD. Mitochondrial DNA sequence analysis from multiple gene fragments reveals genetic heterogeneity of Crassostrea ariakensis in East Asia. Genes Genomics. 2014;36(5):611-24. http://dx.doi.org/10.1007/s13258-014-0198-5.

Lagreze-Squella FJ, Sühnel S, Vieira G, Langdon C, Melo CMR. Optimizing broodstock conditioning for the tropical clam Anomalocardia brasiliana. J Shellfish Res. 2018;37:979-87. http://dx.doi.org/10.2983/035.037.0508.

Lavander H, Santos G, Olivera A, Carvalho R, Guerra M, Coimbra MRM. Meiosis maturation in the marine clam Anomalocardia brasiliana (Veneridae). J Shellfish Res. 2017;36(3):601-5. http://dx.doi.org/10.2983/035.036.0308.

Lavander HD, Cardoso LO Jr, Oliveira RL, Silva SR No, Galvez AO, Peixoto SRM. Biologia reprodutiva da Anomalocardia brasiliana (Gmelin, 1791) no litoral norte de Pernambuco, Brasil. Rev Bras Ciênc Agrár. 2011;6(2):344-50. http://dx.doi.org/10.5039/agraria.v6i2a1139.

Lavander HD, Silva SR No, Sobral SC, Lima PCM, Rêgo MG, Gálvez AO. Manutenção e reprodução de Anomalocardia brasiliana em condições laboratoriais. Rev Bras Ciênc Agrár. 2014;9(2):269-76. http://dx.doi.org/10.5039/agraria.v9i2a3237.

Lettieri G, Mollo V, Ambrosino A, Caccavale F, Troisi J, Febbraio F, Piscopo M. Molecular effects of copper on the reproductive system of Mytilus galloprovincialis. Mol Reprod Dev. 2019;86(10):1357-68. http://dx.doi.org/10.1002/mrd.23114. PMid:30648312.

Lima PCM, Lavander HD, Silva LOB, Gálvez AO. Larviculture of the sand clam cultivated in different densities. Bol Inst Pesca. 2018;44(2):1-7.

Ma P, Wang Z, Yu R. Triploid induction by hyperosmotic shock in the Yesso scallop, Patinopecten yessoensis. J World Aquacult Soc. 2019;50(5):922-33. http://dx.doi.org/10.1111/jwas.12583.

Melo EMC, Gomes CHA M, Silva FC, Sühnel S, Melo CMR. Métodos químico e físico de indução à triploidia em Crassostrea gigas (Thunberg, 1793). Bol Inst Pesca. 2015;41:889-98.

Nowland SJ, O’Connor WA, Elizur A, Southgate PC. Evaluating spawning induction methods for the tropical black-lip rock oyster, Saccostrea echinata. Aquacult Rep. 2021;20:100676. http://dx.doi.org/10.1016/j.aqrep.2021.100676.

Nowland SJ, O’Connor WA, Osborne MWJ, Southgate PC. Current status and potential of tropical rock oyster aquaculture. Rev Fish Sci Aquacult. 2020;28(1):57-70. http://dx.doi.org/10.1080/23308249.2019.1670134.

O’Connor W, Dove M, Finn B, O’Connor S. Manual for hatchery production of Sydney rock oysters (Saccostrea glomerata). Deakin: NSW Department of Primary Industries; 2008. Fisheries research report series nº 20.

Oliveira RLM, Lavander HD, Santos LBG, Calazans NKF, Gálvez AO, Peixoto SRM. Hatchery rearing of the venerid clam Anomalocardia brasiliana (Gmelin, 1791). J Shellfish Res. 2016;35(1):27-30. http://dx.doi.org/10.2983/035.035.0104.

Paixão L, Ferreira MA, Nunes Z, Fonseca-Sizo F, Rocha R. Effects of salinity and rainfall on the reproductive biology of the mangrove oyster (Crassostrea gasar): implications for the collection of broodstock oysters. Aquaculture. 2013;380-383:6-12. http://dx.doi.org/10.1016/j.aquaculture.2012.11.019.

Piferrer F, Beaumont A, Falguière JC, Flajšhans M, Haffray P, Colombo L. Polyploid fish and shellfish: production, biology and applications to aquaculture for performance improvement and genetic containment. Aquaculture. 2009;293(3-4):125-56. http://dx.doi.org/10.1016/j.aquaculture.2009.04.036.

Qin Y, Xiao S, Ma H, Mo R, Zhou Z, Wu X, Zhang Y, Yu Z. Effects of salinity and temperature on the timing of germinal vesicle breakdown and polar body release in diploid and triploid Hong Kong oysters, Crassostrea hongkongensis, in relation to tetraploid induction. Aquacult Res. 2018;49(11):3647-57. http://dx.doi.org/10.1111/are.13833.

Ren J, Hou Z, Wang H, Sun MA, Liu X, Liu B, Guo X. Intraspecific variation in mitogenomes of five Crassostrea species provides insight into oyster diversification and speciation. Mar Biotechnol. 2016;18(2):242-54. http://dx.doi.org/10.1007/s10126-016-9686-8. PMid:26846524.

Rios EC. Compendium of Brazilian sea shells. Rio Grande: Evangraf; 2009.

Salinas-Flores L, Adams SL, Lim MH. Determination of the membrane permeability characteristics of Pacific oyster, Crassostrea gigas, oocytes and development of optimized methods to add and remove ethylene glycol. Cryobiology. 2008;56(1):43-52. http://dx.doi.org/10.1016/j.cryobiol.2007.10.175. PMid:18155687.

Willer DF, Aldridge DC. Sustainable bivalve farming can deliver food security in the tropics. Nat Food. 2020;1(7):384-8. http://dx.doi.org/10.1038/s43016-020-0116-8.

Wood SN. Fast stable restricted maximum likelihood and marginal likelihood estimation of semiparametric generalized linear models. J R Stat Soc Series B Stat Methodol. 2011;73(1):3-36. http://dx.doi.org/10.1111/j.1467-9868.2010.00749.x.

Yang H, Guo X. Triploid hard clams Mercenaria mercenaria produced by inhibiting polar body I or polar body II. Aquacult Res. 2018;49(1):449-61. http://dx.doi.org/10.1111/are.13476.

Zhang Y, Li J, Qin Y, Zhou Y, Zhang Y, Yu Z. A comparative study of the survival, growth and gonad development of the diploid and triploid Hong Kong oyster, Crassostrea hongkongensis (Lam & Morton 2003). Aquacult Res. 2017;48(5):2453-62. http://dx.doi.org/10.1111/are.13081.

Zuur AF, Ieno EN, Walker NJ, Saveliev AA, Smith GM. Mixed effects models and extensions in ecology with R. J Stat Softw. 2009;32:1-3. http://dx.doi.org/10.1007/978-0-387-87458-6.

Zuur AF. A beginner’s guide to generalized additive models with R. Newburgh: Highland Statistics Ltd; 2012.
 


Submitted date:
01/05/2023

Accepted date:
04/13/2023

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