Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.1590/1984-3143-AR2023-0071
Animal Reproduction (AR)
ORIGINAL ARTICLE

Characterization of Brazilian Buriti oil biomaterial: the influence on the physical, chemical properties and behaviour of Goat Wharton’s jelly mesenchymal stem cells

Camila Ernanda Sousa de Carvalho; Fernando da Silva Reis; Elis Rosélia Dutra de Freitas Siqueira Silva; Dayseanny de Oliveira Bezerra; Isnayra Kerolaynne Carneiro Pacheco; Ana Cristina Vasconcelos Fialho; José Milton Elias de Matos; Wanderson Gabriel Gomes de Melo; Yulla Klinger Pereira de Carvalho Leite; Napoleão Martins Argôlo Neto; Maria Acelina Martins de Carvalho

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Abstract

Abstract: The Brazilian Buriti oil presents low extraction costs and relevant antioxidant properties. Thus, this work aimed to analyze Buriti oil biomaterial (BB), within its physicochemical properties, biocompatibility and cellular integration, with the purpose to the use as a growth matrix for Goat Wharton’s jelly mesenchymal stem cells. Biomaterials were produced from Buriti oil polymer (Mauritia flexuosa), for it’s characterization were performed Infrared Region Spectroscopy (FTIR) and Thermogravimetric Analysis (TG and DTG). The biointegration was analyzed by Scanning Electron Microscopy (SEM) and histological techniques. In order to investigate biocompatibility, MTT (3-(4,5-dimetil-2-tiazolil)-2,5-difenil-2H-tetrazólio) test and hemolytic activity tests were performed. The activation capacity of immune system cellswas measured by phagocytic capacity assay and nitric oxide synthesis . The BB presented an amorphous composition, with high thermal stability and high water expansion capacity, a surface with micro and macropores, and good adhesion of Wharton’s jelly mesenchymal stem cells (MSCWJ). We verified the absence of cytotoxicity and hemolytic activity, in addition, BB did not stimulate the activation of macrophages. Proving to be a safe material for direct cultivation and also for manufacturing of compounds used for in vivo applications.

Keywords

cell growth, cytotoxicity, polyurethane

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Submitted date:
05/17/2023

Accepted date:
10/30/2023

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