Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.1590/1984-3143-AR2023-0107
Animal Reproduction (AR)
ORIGINAL ARTICLE

Use of platelet-rich plasma on in vitro maturation during bovine embryo production

Eduardo Baia de Souza; Diego Dubeibe Marin; Anelise Sarges Ramos; Bruno Porpino Homobono; Priscilla do Carmo de Azevedo Ramos; Vanessa Cunha de Brito; Gabriela Santos da Cruz; Nathalia Nogueira da Costa; Marcela da Silva Cordeiro; Simone do Socorro Damasceno Santos

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Abstract

Abstract: One of the crucial aspects to be considered for successful in vitro production (IVP) of embryos is the composition of the various media used throughout the stages of this reproductive biotechnology. The cell culture media employed should fulfill the metabolic requirements of both gametes during oocyte maturation and sperm development, as well as the embryo during its initial cell divisions. Most IVP protocols incorporate blood serum into the media composition as a source of hormones, proteins, growth factors, and nutrients. Numerous studies have suggested Platelet-Rich Plasma (PRP) as a substitute for fetal sera in cell culture, particularly for stem cells. Therefore, the objective of this study is to assess the potential use of PRP as a replacement for fetal bovine serum (FBS) during oocyte maturation for in vitro production of bovine embryos. During in vitro maturation (IVM), cumulus-oocyte complexes (COCs) were allocated into the following experimental groups: Group G1 (IVM medium with 5% PRP); Group G2 (MIV medium with 5% PRP and 5% SFB); Group G3 (MIV medium with 5% SFB); and Group G4 (MIV medium without either PRP or SFB). Subsequently, the cumulus-oocyte complexes were fertilized with semen from a single bull, and the resulting zygotes were cultured for seven days. Cleavage and blastocyst formation rates were assessed on days 2 and 7 of embryonic development, respectively. The quality of matured COCs was also evaluated by analyzing the gene expression of HSP70, an important protein associated with cellular stress. The results demonstrated that there were no significant differences among the experimental groups in terms of embryo production rates, both in the initial cleavage stages and blastocyst formation (except for the G4 group, which exhibited a lower blastocyst formation rate on D7, as expected). This indicates that PRP could be a cost-effective alternative to SFB in the IVP of embryos.

Keywords

embryo, fetal bovine serum, in vitro maturation, platelet rich plasm

References

Abd El-Aziz AH, Mahrous UE, Kamel SZ, Sabek AA. Factors influencing in vitro production of bovine embryos: a review. Asian J Anim Vet Adv. 2016;11(12):737-56. http://dx.doi.org/10.3923/ajava.2016.737.756.

Abdel-Wahab A, Abdel Aziz RL, Helmy NA, Ibrahim SS. The impact of using newborn bovine serum as fetal calf serum substitute in the in vitro bovine embryos production system. Porto Biomed J. 2018;3(2):e3. http://dx.doi.org/10.1016/j.pbj.0000000000000003. PMid:31595239.

Astori G, Amati E, Bambi F, Bernardi M, Chieregato K, Schäfer R, Sella S, Rodeghiero F. Platelet lysate as a substitute for animal serum for the ex-vivo expansion of mesenchymal stem/stromal cells: present and future. Stem Cell Res Ther. 2016;7(1):93. http://dx.doi.org/10.1186/s13287-016-0352-x. PMid:27411942.

Burnouf T, Strunk D, Koh MBC, Schallmoser K. Human platelet lysate: replacing fetal bovine serum as a gold standard for human cell propagation? Biomaterials. 2016;76:371-87. http://dx.doi.org/10.1016/j.biomaterials.2015.10.065. PMid:26561934.

Costa NN, Brito KNL, Santana PPB, Cordeiro MS, Silva TVG, Santos AX, Ramos PC, Santos SSD, King WA, Miranda MS, Ohashi OM. Effect of cortisol on bovine oocyte maturation and embryo development in vitro. Theriogenology. 2019;85(2):323-9. http://dx.doi.org/10.1016/j.theriogenology.2015.08.010. PMid:26456184.

Del Collado M, Saraiva NZ, Lopes FL, Gaspar RC, Padilha LC, Costa RR, Rossi GF, Vantini R, Garcia JM. Influence of bovine serum albumin and fetal bovine serum supplementation during in vitro maturation on lipid and mitochondrial behaviour in oocytes and lipid accumulation in bovine embryos. Reprod Fertil Dev. 2015;28(11):1721-32. http://dx.doi.org/10.1071/RD15067. PMid:25986410.

Dubeibe Marin DF, Costa NN, di Paula Bessa Santana P, Souza EB, Ohashi OM. Importance of lipid metabolism on oocyte maturation and early embryo development: can we apply what we know to buffalo? Anim Reprod Sci. 2019;211:106220. http://dx.doi.org/10.1016/j.anireprosci.2019.106220. PMid:31785645.

Kawamura K, Chen Y, Shu Y, Cheng Y, Qiao J, Behr B, Pera RA, Hsueh AJ. Promotion of human early embryonic development and blastocyst outgrowth in vitro using autocrine/paracrine growth factors. PLoS One. 2012;7(11):e49328. http://dx.doi.org/10.1371/journal.pone.0049328. PMid:23152897.

Marques APL, Botteon RCCM, Cordeiro MD, Machado CH, Botteon PTL, Barros JPN, Spíndola BF. Padronização de técnica manual para obtenção de plasma rico em plaquetas de bovino. Pesq Vet Bras. 2014;34(Supl. 1):1-6. http://dx.doi.org/10.1590/S0100-736X2014001300001.

Maynard DM, Heijnen HFG, Gahl WA, Gunay-Aygun M. The α-granule proteome: novel proteins in normal and ghost granules in gray platelet syndrome. J Thromb Haemost. 2010;8(8):1786-96. http://dx.doi.org/10.1111/j.1538-7836.2010.03932.x. PMid:20524979.

Maynard DM, Heijnen HFG, Horne MK, White JG, Gahl WA. Proteomic analysis of platelet a-granules using mass spectrometry. J Thromb Haemost. 2007;5(9):1945-55. http://dx.doi.org/10.1111/j.1538-7836.2007.02690.x. PMid:17723134.

Paradkar PH, Db Vaidya A, Talwalkar SC, Mishra LS, Agashe SV, Vaidya RA. Bovine whey protein and other biological fluids as alternative to fetal bovine serum in supplementing cell culture media. Indian J Exp Biol. 2019;57(2):123-30.

Pazoki H, Eimani H, Farokhi F, Shahverdi AH, Tahaei LS. The effects of platelet lysate on maturation, fertilization and embryo development of NMRI mouse oocytes at germinal vesicle stage. Reprod Med Biol. 2015;15(2):115-20. http://dx.doi.org/10.1007/s12522-015-0220-x. PMid:29259427.

Richani D, Gilchrist RB. The epidermal growth factor network: role in oocyte growth, maturation and developmental competence. Hum Reprod Update. 2018;24(1):1-14. http://dx.doi.org/10.1093/humupd/dmx029. PMid:29029246.

Rosenzweig R, Nillegoda NB, Mayer MP, Bukau B. The Hsp70 chaperone network. Nat Rev Mol Cell Biol. 2019;20(11):665-80. http://dx.doi.org/10.1038/s41580-019-0133-3. PMid:31253954.

Shan Q, Ma F, Wei J, Li H, Ma H, Sun P. Physiological functions of heat shock proteins. Curr Protein Pept Sci. 2020;21(8):751-60. http://dx.doi.org/10.2174/1389203720666191111113726. PMid:31713482.

Stamperna K, Giannoulis T, Dovolou E, Kalemkeridou M, Nanas I, Dadouli K, Moutou K, Mamuris Z, Amiridis GS. Heat shock protein 70 improves in vitro embryo yield and quality from heat stressed bovine oocytes. Animals. 2021;11(6):1794. http://dx.doi.org/10.3390/ani11061794. PMid:34208520.

Stroebech L, Mazzoni G, Pedersen HS, Freude KK, Kadarmideen HN, Callesen H, et al. In vitro production of bovine embryos: revisiting oocyte development and application of systems biology. Anim Reprod. 2015;12(3):465-72.

Sundman EA, Cole BJ, Fortier LA. Growth factor and catabolic cytokine concentrations are influenced by the cellular composition of platelet-rich plasma. Am J Sports Med. 2011;39(10):2135-40. http://dx.doi.org/10.1177/0363546511417792. PMid:21846925.

Tobita M, Tajima S, Mizuno H. Adipose tissue-derived mesenchymal stem cells and platelet-rich plasma: stem cell transplantation methods that enhance stemness. Stem Cell Res Ther. 2015;6:215. http://dx.doi.org/10.1186/s13287-015-0217-8. PMid:26541973.

Warzych E, Wrenzycki C, Peippo J, Lechniak D. Maturation medium supplements affect transcript level of apoptosis and cell survival related genes in bovine blastocysts produced in vitro. Mol Reprod Dev. 2007;74(3):280-9. http://dx.doi.org/10.1002/mrd.20610. PMid:16955406.
 


Submitted date:
07/03/2023

Accepted date:
02/19/2024

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