Animal Reproduction (AR)
Animal Reproduction (AR)

Padi6 expression patterns in buffalo oocytes and preimplantation embryos

Qinqiang Sun; Yuan Yang; Yue Zhang; Dongrong Chen; Haiying Zheng; Guangsheng Qin; Qiang Fu

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Abstract: The subcortical maternal complex, which consists of maternal-effect genes, plays a crucial role in the development of oocytes and preimplantation embryo until the activation of the zygote genome. One such gene, known as peptidyl-arginine deiminase VI (Padi6), is involved in the oocyte maturation, fertilization and embryonic development. However, the precise function of Padi6 gene in buffalo is still unclear and requires further investigation. In this study, the sequence, mRNA and protein expression patterns of Padi6 gene were analyzed in oocytes, preimplantation embryos and somatic tissues of buffalo. The coding sequence of gene was successfully cloned and characterized. Real-time quantitative PCR results indicated an absence of Padi6 transcripts in somatic tissues. Notably, the expression levels of Padi6 in oocytes showed an increased from the germinal vesicle stage to metaphase II stage, followed by a rapid decrease during the morula and blastocyst stages. Immunofluorescence analysis confirmed these findings, revealing a noticeable decline in protein expression levels. Our research provides the initial comprehensive expression profile of Padi6 in buffalo oocytes and preimplantation embryos, serving as a solid foundation for further investigations into the functionality of maternal-effect genes in buffalo.


maternal-effect genes, Padi6, oocyte, preimplantation embryo, immunofluorescence


Chavanas S, Mechin MC, Takahara H, Kawada A, Nachat R, Serre G, Simon M. Comparative analysis of the mouse and human peptidylarginine deiminase gene clusters reveals highly conserved non-coding segments and a new human gene, PADI6. Gene. 2004;330:19-27. PMid:15087120.

Esposito G, Vitale AM, Leijten FPJ, Strik AM, Koonen-Reemst AMCB, Yurttas P, Robben TJAA, Coonrod S, Gossen JA. Peptidylarginine deiminase (PAD) 6 is essential for oocyte cytoskeletal sheet formation and female fertility. Mol Cell Endocrinol. 2007;273(1-2):25-31. PMid:17587491.

Evsikov AV, Marín de Evsikova C. Gene expression during the oocyte-to-embryo transition in mammals. Mol Reprod Dev. 2009;76(9):805-18. PMid:19363788.

Fu Q, Liu ZF, Huang YL, Lu YQ, Zhang M. Comparative proteomic analysis of mature and immature oocytes of the swamp buffalo (Bubalus bubalis). Int J Mol Sci. 2016;17(1):94. PMid:26784167.

Gasteiger E, Hoogland C, Gattiker A, Duvaud S, Wilkins MR, Appel RD, Bairoch A. Protein identification and analysis tools on the ExPASy server. In: Walker JM, editor. The proteomics protocols handbook. Nova Jersey: Humana Press; 2005. Springer Protocols Handbooks.

Huang YL, Wang HJ, Chen FM, Zhao XL, Fu Q, Zhang PF, Pu LP, Huang FL, Lu YQ, Zhang M. Role of BCL2L10 in regulating buffalo (Bubalus bubalis) oocyte maturation. Theriogenology. 2018;110:1-7. PMid:29331495.

Jukam D, Shariati SAM, Skotheim JM. Zygotic genome activation in vertebrates. Dev Cell. 2017;42(4):316-32. PMid:28829942.

Lu X, Gao Z, Qin D, Li L. A maternal functional module in the mammalian oocyte-to-embryo transition. Trends Mol Med. 2017;23(11):1014-23. PMid:28993030.

Maddirevula S, Coskun S, Awartani K, Alsaif H, Abdulwahab FM, Alkuraya FS. The human knockout phenotype of PADI6 is female sterility caused by cleavage failure of their fertilized eggs. Clin Genet. 2017;91(2):344-5. PMid:27730629.

Mahadevan S, Sathappan V, Utama B, Lorenzo I, Kaskar K, Vanden VIB. Maternally expressed NLRP2 links the subcortical maternal complex (SCMC) to fertility, embryogenesis and epigenetic reprogramming. Sci Rep. 2017;7(1):44667. PMid:28317850.

Monk D, Sanchez-Delgado M, Fisher R. NLRPs, the subcortical maternal complex and genomic imprinting. Reproduction. 2017;154(6):R161-70. PMid:28916717.

Pennetier S, Perreau C, Uzbekova S, Thelie A, Delaleu B, Mermillod P, Dalbies-Tran R. MATER protein expression and intracellular localization throughout folliculogenesis and preimplantation embryo development in the bovine. BMC Dev Biol. 2006;6(1):26. PMid:16753072.

Pisani LF, Ramelli P, Lazzari B, Braglia S, Ceciliani F, Mariani P. Characterization of maternal antigen that embryos require (MATER/NLRP5) gene and protein in pig somatic tissues and germ cells. J Reprod Dev. 2010;56(1):41-8. PMid:19815987.

Qian J, Nguyen NMP, Rezaei M, Huang B, Tao YL, Zhang XF, Cheng Q, Yang HJ, Asangla A, Majewski J, Slim R. Biallelic PADI6 variants linking infertility, miscarriages, and hydatidiform moles. Eur J Hum Genet. 2018;26(7):1007-13. PMid:29693651.

Rezaei M, Suresh B, Bereke E, Hadipour Z, Aguinaga M, Qian JH, Bagga R, Fardaei M, Hemida R, Jagadeesh S, Majewski J, Slim R. Novel pathogenic variants in NLRP7, NLRP5, and PADI6 in patients with recurrent hydatidiform moles and reproductive failure. Clin Genet. 2021;99(6):823-8. PMid:33583041.

Witalison EE, Thompson PR, Hofseth LJ. Protein arginine deiminases, associated citrullination: physiological functions and diseases associated with dysregulation. Curr Drug Targets. 2015;16(7):700-10. PMid:25642720.

Xu Y, Shi Y, Fu J, Yu M, Feng R, Sang Q, Liang B, Chen B, Qu R, Li B, Yan Z, Mao X, Kuang Y, Jin L, He L, Sun X, Wang L. Mutations in PADI6 cause female infertility characterized by early embryonic arrest. Am J Hum Genet. 2016;99(3):744-52. PMid:27545678.

Yu XJ, Yi Z, Gao Z, Qin D, Zhai Y, Chen X, Ou-Yang Y, Wang ZB, Zheng P, Zhu MS, Wang H, Sun QY, Dean J, Li L. The subcortical maternal complex controls symmetric division of mouse zygotes by regulating F-actin dynamics. Nat Commun. 2014;5(1):4887. PMid:25208553.

Yurttas P, Vitale AM, Fitzhenry RJ, Cohen-Gould L, Wu W, Gossen JA, Coonrod SA. Role for PADI6 and the cytoplasmic lattices in ribosomal storage in oocytes and translational control in the early mouse embryo. Development. 2008;135(15):2627-36. PMid:18599511.

Zhang K, Smith GW. Maternal control of early embryogenesis in mammals. Reprod Fertil Dev. 2015;27(6):880-96. PMid:25695370.

Zhang M, Liu C, Chen B, Lv M, Zou H, Liu Y, Ga Y, Wang T, Xing Q, Zhu Y. Identification of novel biallelic TLE6 variants in female infertility with preimplantation embryonic lethality. Front Genet. 2021;12:666136. PMid:34178031.

Zhu K, Yan L, Zhang X, Lu X, Wang T, Yan J, Liu X, Qiao J, Li L. Identification of a human subcortical maternal complex. Mol Hum Reprod. 2015;21(4):320-9. PMid:25542835.

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