Evaluation of the morphology and development of preantral ovarian follicles in mice submitted to a chronic diet of dietary supplementation with Pereskia aculeata Miller leaves

Alesandro Silva Ferreira; Francisco Glauber Peixoto Ferreira; Etho Roberio Medeiros Nascimento; Gildas Mbemya Tetaping; Laritza Ferreira de Lima; Said Gonçalves da Cruz Fonseca; José Ricardo de Figueiredo; Daniel Freire de Sousa; Juliana Jales de Hollanda Celestino

doi:10.1590/1984-3143-AR2024-0012

SHORT COMMUNICATION

Evaluation of the morphology and development of preantral ovarian follicles in mice submitted to a chronic diet of dietary supplementation with Pereskia aculeata Miller leaves

Alesandro Silva Ferreira; Francisco Glauber Peixoto Ferreira; Etho Roberio Medeiros Nascimento; Gildas Mbemya Tetaping; Laritza Ferreira de Lima; Said Gonçalves da Cruz Fonseca; José Ricardo de Figueiredo; Daniel Freire de Sousa; Juliana Jales de Hollanda Celestino

http://dx.doi.org/10.1590/1984-3143-AR2024-0012 Anim Reprod, vol.21, n2, e20240012, 2024

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Abstract

This study aimed to investigate the effect of including mouse feed with different concentrations (5, 10, or 20%) of Pereskia aculeata Miller (PAM) leaves on the morphology and development of preantral ovarian follicles and ovarian stromal cell density. The oral toxicity was performed using repeated dose toxicity assays subdivided into experiments of 30 days and 90 days of treatment. After the experiments, the ovaries of each animal were collected and submitted to classical histology. At 30 and 90 days, there was an equivalent percentage of normal, primordial, and developing follicles (P > 0.05) between PAM treatments compared to the control. Regarding the different stages of follicular development, after 90 days, there was a higher percentage (P < 0.05) of developing follicles only in the control group compared to day 30. The PAM 5% treatment was the only one that affected the cell density in the stroma after 90 days of treatment. Thus, we observed that supplementing the diet with P. aculeata did not pose any risk concerning animal consumption; specifically, there were no toxic reproductive effects observed from adding Pereskia aculeata Miller to the mouse diet.

Keywords

animal feed, reproductive toxicity, ovarian follicles, medicinal plants, natural products

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Submitted date:
02/01/2024

Accepted date:
05/20/2024

Evaluation of the morphology and development of preantral ovarian follicles in mice submitted to a chronic diet of dietary supplementation with Pereskia aculeata Miller leaves

Alesandro Silva Ferreira; Francisco Glauber Peixoto Ferreira; Etho Roberio Medeiros Nascimento; Gildas Mbemya Tetaping; Laritza Ferreira de Lima; Said Gonçalves da Cruz Fonseca; José Ricardo de Figueiredo; Daniel Freire de Sousa; Juliana Jales de Hollanda Celestino

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