Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.1590/1984-3143-AR2024-0018
Animal Reproduction (AR)
ORIGINAL ARTICLE

Investigating the safety of antibiotics added to collared peccary (Pecari tajacu) semen extender through a multiparametric thermoresistance test

Caio Sérgio Santos; Yasmim Carla da Silva Cavalcante; Lívia Batista Campos; Andréia Maria da Silva; Francisco Marlon Carneiro Feijó; Alexandre Rodrigues Silva

http://dx.doi.org/10.1590/1984-3143-AR2024-0018 Anim Reprod, vol.21, n3, e20240018, 2024
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Abstract

The effects of antibiotics on sperm longevity in collared peccary (Pecari tajacu) fresh diluted semen was evaluated. Semen samples from six adult males were collected by electroejaculation and diluted in Tris-citrate-fructose alone (control) and plus streptomycin-penicillin (2 mg/ml-2000 IU/ml) or gentamicin (70 µg/ml). Membrane integrity and functionality, mitochondrial activity and sperm morphology were assessed subjectively. Sperm motility and other kinetic parameters were objectively assessed using CASA (computer-assisted semen analysis). The semen diluted according to the treatments were submitted to the thermoresistance test, incubated at 37 ° C, and the sperm parameters analyzed at 0, 30, 60, 120 and 180 min. The average values of the treatments were compared with each other and between the times. There were no differences (P > 0.05) between treatments until the end of the test. Control and streptomycin-penicillin samples maintained sperm function for up to 180 min (with total motility of 24.3 ± 7.1% and 28 ± 8.7%, respectively). Gentamicin aliquots retained most parameters until the end of the incubation, except for membrane integrity and mitochondrial activity that declined (P < 0.05) at 180 min (53.1 ± 7.1% and 50.7 ± 6.2%, respectively) compared to 0 min (80.5 ± 4.7% and 86.3 ± 3.4%, respectively). In conclusion, a multiparametric thermoresistance test proved that Tris-based extenders used for collared peccary semen can be effectively supplemented by streptomycin-penicillin (2 mg/ml-2000 IU/ml) or gentamicin (70 µg/ml), especially during 180-min incubation at 37 °C.

Keywords

antibacterial, extender, sperm, thermoresistance, toxicity

References

Akhter S, Ansari MS, Andrabi SMH, Ullah N, Qayyum M. Effect of antibiotics in extender on bacterial and spermatozoal quality of cooled buffalo (Bubalus bubalis) bull semen. Reprod Domest Anim. 2008;43(3):272-8. http://doi.org/10.1111/j.1439-0531.2007.00890.x. PMid:18042206.

Almquist JO. A comparison of Penicillin, Streptomycin and sulfanilamide for improving the fertility of semen from bulls of low fertility. J Dairy Sci. 1951;34(8):819-22. http://doi.org/10.3168/jds.S0022-0302(51)91787-0.

Aurich C, Spergser J. Influence of bacteria and gentamicin on cooled-stored stallion spermatozoa. Theriogenology. 2007;67(5):912-8. http://doi.org/10.1016/j.theriogenology.2006.11.004. PMid:17141306.

Bonet S, Delgado-Bermúdez A, Yeste M, Pinart E. Study of boar sperm interaction with Escherichia coli and Clostridium perfringens in refrigerated semen. Anim Reprod Sci. 2018;197:134-44. http://doi.org/10.1016/j.anireprosci.2018.08.021. PMid:30143281.

Bryła M, Trzcińska M. Quality and fertilizing capacity of boar spermatozoa during liquid storage in extender supplemented with different antibiotics. Anim Reprod Sci. 2015;163:157-63. http://doi.org/10.1016/j.anireprosci.2015.11.005. PMid:26586542.

Campos LB, Silva MA, Bezerra JAB, Castelo TS, Peixoto GCX, Silva AR. Survival of collared peccaries (Pecari tajacu) sperm after freezing-thawing using different extenders. Acta Sci Vet. 2014;42:1217.

Castelo TS, Bezerra FSB, Souza ALP, Moreira MAP, Paula VV, Oliveira MF, Silva AR. Influence of the thawing rate on the cryopreservation of semen from collared peccaries (Tayassu tajacu) using Tris-based extenders. Theriogenology. 2010;74(6):1060-5. http://doi.org/10.1016/j.theriogenology.2010.05.002. PMid:20580079.

Dean CJ, Hobgood AM, Blodgett GP, Love CC, Blanchard TL, Varner DD. The addition of ticarcillin-clavulanic acid to INRA 96 extender for stallion semen cooling. Equine Vet J. 2012;44(S43):95-9. http://doi.org/10.1111/j.2042-3306.2012.00638.x. PMid:23447886.

Desbiez ALJ, Keuroghlian A, Beisiegel BM, Medici EP, Gatti A, Pontes ARM, Campos CB, Tófoli CF, Moraes EA Jr, Azevedo FC, Pinho GM, Cordeiro JLP, Santos TS Jr, Morais AA, Mangini PR, Flesher K, Rodrigues LF, Almeida LB. Avaliação do risco de extinção do cateto Pecari tajacu Linnaeus, 1758, no Brasil. Biod Bras [serial on the Internet]. 2012 [cited 2024 Feb 9];2(1):74-83. Available from: https://revistaeletronica.icmbio.gov.br/BioBR/article/view/241

Elliott RL, Jiang XP. The adverse effect of gentamicin on cell metabolism in three cultured mammary cell lines: “are cell culture data skewed?”. PLoS One. 2019;14(4):e0214586. http://doi.org/10.1371/journal.pone.0214586. PMid:30934018.

Gongora J, Reyna-Hurtado R, Beck H, Taber A, Altrichter M, Keuroghlian A. Pecari tajacu. In: International Union for Conservation of Nature and Natural Resources – IUCN. The IUCN Red List of Threatened Species 2011: e. T41777A10562361. RLTS T41777A10562361. Cambridge: IUCN; 2011.

Hahn FE, Sarre SG. Mechanism of action of gentamicin. J Infect Dis. 1969;119(4):364-9. http://doi.org/10.1093/infdis/119.4-5.364. PMid:4892389.

Kalghatgi S, Spina CS, Costello JC, Liesa M, Morones-Ramirez JR, Slomovic S, Molina A, Shirihai OS, Collins JJ. Bactericidal antibiotics induce mitochondrial dysfunction and oxidative damage in mammalian cells. Sci Transl Med. 2013;5(192):192ra85. http://doi.org/10.1126/scitranslmed.3006055. PMid:23825301.

Long S, Woodward B, Tomlinson M. Sperm toxicity testing: UK best practice guideline from the Association of Biomedical Andrologists. Br J Biomed Sci. 2018;75(2):53-60. http://doi.org/10.1080/09674845.2017.1377591. PMid:29421949.

Lopes G, Simões A, Ferreira P, Martins-Bessa A, Rocha A. Differences in preservation of canine chilled semen using different transport containers. Anim Reprod Sci. 2009;112(1–2):158-63. http://doi.org/10.1016/j.anireprosci.2008.04.003. PMid:18479849.

Luzzatto L, Apirion D, Schlessinger D. Mechanism of action of streptomycin in E. coli: interruption of the ribosome cycle at the initiation of protein synthesis. Proc Natl Acad Sci USA. 1968;60(3):873-80. http://doi.org/10.1073/pnas.60.3.873. PMid:4875806.

Moreira SSJ, Santos CS, Castelo TS, Bezerra LGP, Praxedes ECG, Matos TM, Souza-Junior JBF, Feijó FMC, Comizzoli P, Silva AR. Investigating the need for antibiotic supplementation to the extender used for semen cryopreservation in collared peccaries. Front Vet Sci. 2022;9:954921. http://doi.org/10.3389/fvets.2022.954921. PMid:36118351.

Moustacas VS, Xavier MN, Carvalho-Júnior CA, Costa EA, Henry M, Santos RL. Effect of extender supplementation with various antimicrobial agents on viability of Brucella ovis and Actinobacillus seminis in cryopreserved ovine semen. Theriogenology. 2010;74(8):1476-81. http://doi.org/10.1016/j.theriogenology.2010.06.019. PMid:20708246.

Peixoto GCX, Lima GL, Maia KM, Souza ALP, Castelo TS, Paiva ALC, Paula VV, Oliveira MF, Brito AB, Domingues SFS, Viana ACNPCS, Melo LM, Comizzoli P, Silva AR. Single injection of eCG/hCG leads to successful estrous synchronization in the collared peccary (Pecari tajacu Linnaeus, 1758). Anim Reprod Sci. 2019;208:106112. http://doi.org/10.1016/j.anireprosci.2019.106112. PMid:31405481.

Pinart E, Domènech E, Bussalleu E, Yeste M, Bonet S. A comparative study of the effects of Escherichia coli and Clostridium perfringens upon boar semen preserved in liquid storage. Anim Reprod Sci. 2017;177:65-78. http://doi.org/10.1016/j.anireprosci.2016.12.007. PMid:27988080.

Rodriguez-Martinez H, Barth AD. In vitro evaluation of sperm quality related to in vivo function and fertility. Soc Reprod Fertil Suppl. 2007;64:39-54. http://doi.org/10.5661/RDR-VI-39. PMid:17494215.

Santos CS, Campos LB, Praxedes ÉCG, Moreira SSJ, Souza-Júnior JBF, Comizzoli P, Feijó FMC, Silva AR. Influence of antibiotics on bacterial load and sperm parameters during short-term preservation of collared peccary semen. Anim Reprod. 2021;18(3):e20210021. http://doi.org/10.1590/1984-3143-ar2021-0021. PMid:34539871.

Santos CS, Silva AM, Maia KM, Rodrigues GSO, Feijó FMC, Alves ND, Oliveira MF, Silva AR. Composition of semen and foreskin mucosa aerobic microbiota and its impact on sperm parameters of captive collared peccaries (Pecari tajacu). J Appl Microbiol. 2020;129(3):521-31. http://doi.org/10.1111/jam.14663. PMid:32281210.

Santos E, Sousa P, Peixoto G, Simão B, Oliveira M, Silva A. Establishing the hypoosmotic swelling test for sperm analysis in collared peccaries (Pecari tajacu). Arq Bras Med Vet Zootec. 2013;65(4):1257-60. http://doi.org/10.1590/S0102-09352013000400044.

Santos EAA, Sousa PC, Dias CEV, Castelo TS, Peixoto GCX, Lima GL, Ricarte AR, Simão BR, Freitas CI, Silva AR. Assessment of sperm survival and functional membrane integrity of the six-banded armadillo (Euphractus sexcinctus). Theriogenology. 2011;76(4):623-9. http://doi.org/10.1016/j.theriogenology.2011.03.015. PMid:21565395.

Santos MVO, Silva AM, Aquino LVC, Oliveira LRM, Moreira SSJ, Oliveira MF, Silva AR, Pereira AF. Different methods for seminal plasma removal and sperm selection on the quality and fertility of collared peccary sperm. Animals. 2023;13(12):1955. http://doi.org/10.3390/ani13121955. PMid:37370465.

Silva A, Peixoto G, Bezerra J, Castelo TS, Santos EAA, Silva AR. Relações entre a câmara de Neubauer a espectrofotometria utilizadas para a determinação da concentração espermática de catetos (Pecari tajacu). Cienc Rural. 2014;44(8):1494-8. http://doi.org/10.1590/0103-8478cr20130928.

Silva AM, Santos RP, Matos YG, Silva AR. Recuperação, caracterização e criopreservação de espermatozoides epididimários de animais silvestres do bioma Caatinga. Rev Bras Reprod Anim. 2023;47(2):171-8. http://doi.org/10.21451/1809-3000.RBRA2023.025.

Silva PFN, Gadella BM. Detection of damage in mammalian sperm cells. Theriogenology. 2006;65(5):958-78. http://doi.org/10.1016/j.theriogenology.2005.09.010. PMid:16242762.

Sousa PC, Santos EAA, Souza ALP, Lima GL, Barros FFPC, Oliveira MF, Silva AR. Sperm morphological and morphometric evaluation in captive collared peccaries (Pecari tajacu). Pesq Vet Bras. 2013;33(7):924-30. http://doi.org/10.1590/S0100-736X2013000700014.

Souza ALP, Castelo TS, Queiroz JPAF, Barros IO, Paula VV, Oliveira MF, Silva AR. Evaluation of anesthetic protocol for the collection of semen from captive collared peccaries (Tayassu tajacu) by electroejaculation. Anim Reprod Sci. 2009;116(3–4):370-5. http://doi.org/10.1016/j.anireprosci.2009.02.017. PMid:19327920.

Souza ALP, Lima GL, Peixoto GCX, Silva AM, Oliveira MF, Silva AR. Use of Aloe vera–based extender for chilling and freezing collared peccary (Pecari tajacu) semen. Theriogenology. 2016;85(8):1432-8. http://doi.org/10.1016/j.theriogenology.2016.01.007. PMid:26830302.

Waberski D, Luther AM, Grünther B, Jäkel H, Henning H, Vogel C, Peralta W, Weitze KF. Sperm function in vitro and fertility after antibiotic-free, hypothermic storage of liquid preserved boar semen. Sci Rep. 2019;9(1):14748. http://doi.org/10.1038/s41598-019-51319-1. PMid:31611589.

Waxman DJ, Strominger JL. Penicillin-binding proteins and the mechanism of action of β-lactam antibiotics. Annu Rev Biochem. 1983;52(1):825-69. http://doi.org/10.1146/annurev.bi.52.070183.004141. PMid:6351730.

Zindl C, Asa CS, Günzel-Apel AR. Influence of cooling rates and addition of Equex pasta on cooled and frozen-thawed semen of generic gray (Canis lupus) and Mexican gray wolves (C. l. baileyi). Theriogenology. 2006;66(6-7):1797-802. http://doi.org/10.1016/j.theriogenology.2006.01.023. PMid:16472849.
 

Submitted date:
02/09/2024

Accepted date:
06/04/2024

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