Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.1590/1984-3143-AR2024-0020
Animal Reproduction (AR)
ORIGINAL ARTICLE

Exploring Myrciaria dubia liquid extract as a potential semen extender for breeding roosters

Marcia Lorena Monteiro da Silva; João Paulo Ferreira Rufino; Brenda de Meireles Lima; Marco Antonio de Freitas Mendonça; Francisco Alberto de Lima Chaves; Roseane Pinto Martins de Oliveira; Pedro de Queiroz Costa Neto; Paulo Cesar Machado Andrade

Downloads: 0
Views: 86

Abstract

The current investigation aimed to explore the effects of Myrciaria dubia liquid extract (MDLE) as the primary component of an extender for breeder rooster semen over different periods at room temperature. Fifteen breeder roosters (40 weeks of age, average body weight of 2.05±0.12) with confirmed fertility were used. Employing a factorial design (3x4), the treatments consisted of semen in natura and two semen extenders (an experimental based on MDLE and a commercial) subjected to four periods at room temperature post-collection (5, 10, 15 and 20 minutes) with four replicates (tubes) each. All variables evaluated in this study yielding significant results (p<0.05). Analyzed individually, the experimental extender based on MDLE exhibited a linear reduction (p<0.05) in motility and vigor results, while it caused an increase in pH values and percentages of sperm defects evaluated. When compared with semen in natura and commercial extender, the efficiency of MDLE as a semen extender was inferior to that observed with the commercial extender and similar to the results observed with semen in natura. Nonetheless, the experimental extender based on MDLE yielded satisfactory results for up to 15 minutes of storage time. In conclusion, MDLE can be considered as an alternative for composing a roosters’ semen extender, maintaining sperm characteristics within acceptable limits for up to 15 minutes at room temperature. However, this experimental extender demonstrated lower efficiency than the commercial extender in maintaining the sperm quality at room temperature across all periods tested.

Keywords

camu-camu, diluent, Myrciaria dubia, semen extender, semen quality

References

Agostinho RFA, Andrade VAA, Caiado RPS, Barreto MAP, Caiado JRC, Shimoda E, Silva JFS. The addition of salmon oil in the freezing of equine semen. Rev Bras Saúde Prod Anim. 2017;18(4):604-9. http://doi.org/10.1590/s1519-99402017000400011.

Akachi T, Shiina Y, Kawaguchi T, Kawagishi H, Morita T, Sugiyama K. 1-methyl malate from Camu-camu (Myrciaria dubia) suppressed D-galactosamine-induced liver injury in rats. Biosci Biotechnol Biochem. 2010;74(3):573-8. http://doi.org/10.1271/bbb.90775. PMid:20208347.

Alkali IM, Asuku SO, Colombo M, Bukar MM, Waziri MA, Luvoni GC. Spermatozoa survival in egg yolk‐based and soybean‐based extenders at ambient and chilling temperature in domestic turkeys (Meleagris gallopavo). Animals (Basel). 2022;12(5):648. http://doi.org/10.3390/ani12050648. PMid:35268216.

Balogun AS, Akinosun AA, Boladuro BA, Tiamiyu AK, Oluwemimo BA. Optimizing dilution rate of cock semen with natural based extender for optimum fertility. Archive of Science and Technology. 2020;1:175-82.

Bellagamba F, Cerolini S, Cavalchini LG. Cryopreservation of poultry semen: a review. Worlds Poult Sci J. 2007;49(2):157-66. http://doi.org/10.1079/WPS19930013.

Blesbois E. Current status in avian semen cryopreservation. Worlds Poult Sci J. 2007;63(2):213-22. http://doi.org/10.1017/S0043933907001419.

Blom E. The morphological estimation of the spermatozoa defects of bull II. The proposal of new classification of spermatozoa defects. Med Welt. 1981;37:239-42.

Bogdonoff PD Jr, Shaffner CS. The effect of pH on the in vitro survival, metabolic activity, and fertilizing capacity of chicken semen. Poult Sci. 1954;33(4):665-9. http://doi.org/10.3382/ps.0330665.

Bongalhardo DC, Leeson S, Buhr MM. Dietary lipids differentially affect membranes from different areas of rooster sperm. Poult Sci. 2009;88(5):1060-9. http://doi.org/10.3382/ps.2008-00392. PMid:19359696.

Bongalhardo DC. Production and preservation of rooster semen. Rev Bras Reprod Anim. 2013;37:131-5.

Brazilian College of Animal Reproduction. Manual for andrological examination and evaluation of animal semen (3rd ed.). Belo Horizonte: Brazilian College of Animal Reproduction; 2013. [in Portuguese].

Brillard JP, McDaniel GR. The reliability and efficiency of various methods for estimating spermatozoa concentration. Poult Sci. 1985;64(1):155-8. http://doi.org/10.3382/ps.0640155. PMid:3975194.

Burrows WH, Quinn JP. The collection of spermatozoa from the domestic fowl and Turkey. Poult Sci. 1937;16(1):19-24. http://doi.org/10.3382/ps.0160019.

Bustani GS, Baiee FH. Semen extenders: an evaluative overview of preservative mechanisms of semen and semen extenders. Vet World. 2021;14(5):1220-33. http://doi.org/10.14202/vetworld.2021.1220-1233. PMid:34220124.

Carmo MAVD, Fidelis M, Pressete CG, Marques MJ, Castro-Gamero AM, Myoda T, Granato D, Azevedo L. Hydroalcoholic Myrciaria dubia (camu-camu) seed extracts prevent chromosome damage and act as antioxidant and cytotoxic agents. Food Res Int. 2019;125:108551. http://doi.org/10.1016/j.foodres.2019.108551. PMid:31554128.

Chatterjee S, Hadi AS. Regression Analysis by Example (4th ed.). New Jersey, US: John Wiley & Sons Ltd; 2006. http://doi.org/10.1002/0470055464.

Chirinos R, Galarza J, Betalleluz-Pallardel I, Pedreschi R, Campos D. Antioxidant compounds and antioxidante capacity of peruvian camu-camu (Myrciaria dubia (H.B.K.) McVaugh) fruit at different maturity stages. Food Chem. 2010;120(4):1019-24. http://doi.org/10.1016/j.foodchem.2009.11.041.

Craft WA, McElory VH, Penquite R. The influence of certain feeds upon the production of spematozoa by the domestic chicken. Poult Sci. 1926;5(4):187-9. http://doi.org/10.3382/ps.0050187.

Dormann CF, Elith J, Bacher S, Buchmann C, Carl G, Carré G, Marquéz JRG, Gruber B, Lafourcade B, Leitão PJ, Münkemüller T, Mcclean C, Osborne PE, Reineking B, Schröder B, Skidmore AK, Zurell D, Lautenbach S. Collinearity: a review of methods to deal with it and a simulation study evaluating their performance. Ecography. 2013;36(1):27-46. http://doi.org/10.1111/j.1600-0587.2012.07348.x.

Feijo JC, Cruz FGG, Melo RD, Rufino JPF, Dias ECS, Brandao ABT. Reproductive evaluation and performance of progeny in the initial phase of semi-heavy cocks with different body weights. Arch Vet Sci. 2016;21:11-8.

Fidelis M, Santos JS, Escher GB, do Carmo MV, Azevedo L, Silva MC, Putnik P, Granato D. In vitro antioxidant and antihypertensive compounds from camu-camu (Myrciaria dubia McVaugh, Myrtaceae) seed coat: A multivariate structure-activity study. FCT. 2018;120:479-90. http://doi.org/10.1016/j.fct.2018.07.043. PMid:30055315.

Freitas BKM, Cruz FGG, Rufino JPF, Feijó JC, Melo RD, Melo LD. Powdered coconut water as preservant of semi-heavy cocks semen. Rev Bras Saúde Prod Anim. 2018;19(2):216-22. http://doi.org/10.1590/s1519-99402018000200007.

Horszwald A, Andlauer W. Characterisation of bioactive compounds in berry juices by traditional photometric and modern microplate methods. J Berry Res. 2011;1(4):189-99. http://doi.org/10.3233/JBR-2011-020.

Jarinkovičová L, Máchal L, Máchal J, Filipčík R, Tůmová E, Horský R. Relationship of ejaculate quality and selected biochemical parameters of blood in cockerels of three laying lines. Czech J Anim Sci. 2012;57(8):370-6. http://doi.org/10.17221/6272-CJAS.

Kanatiyanont N, Kornkaewrat K, Suthanmapinunt P, Pinyopummin A. Effect of semen collection techniques on semen quality and sperm motility parameters in Siamese fighting cock (Gallus gallus). Wetchasan Sattawaphaet. 2012;42(4):439-45. http://doi.org/10.56808/2985-1130.2422.

Khan RU. Antioxidants and poultry semen quality. Worlds Poult Sci J. 2011;67(2):297-308. http://doi.org/10.1017/S0043933911000316.

Lavor CT, Câmara SR. Semen biotechnology and avian artificial insemination. Ciênc Anim. 2012;22:66-81. [UECE]

Layek SS, Mohanty TK, Kumaresan A, Parks JE. Cryopreservation of bull semen: evolution from egg yolk based to soybean-based extenders. Anim Reprod Sci. 2016;172:1-9. http://doi.org/10.1016/j.anireprosci.2016.04.013. PMid:27509873.

Logan M. Biostatistical design and analysis using R: a practical guide. New Jersey, US: John Wiley & Sons Ltd; 2010. http://doi.org/10.1002/9781444319620.

Lotfi S, Mehri M, Sharafi M, Masoudi R. Hyaluronic acid improves frozen-thawed sperm quality and fertility potential in rooster. Anim Reprod Sci. 2017;184:204-10. http://doi.org/10.1016/j.anireprosci.2017.07.018. PMid:28781155.

Lukaszewicz E, Jerysz A, Partyka A, Siudzinska A. Efficacy of evaluation of rooster sperm morphology using different straining methods. Res Vet Sci. 2008;85(3):583-8. http://doi.org/10.1016/j.rvsc.2008.03.010. PMid:18486956.

Marco-Jiménez, F., Puchades, S., Mocé, E., Viudes-de-Cartro, M.P., Vicente, J.S., Rodriguez, M. Use of powdered egg yolk vs fresh egg yolk for the cryopreservation of ovine semen. Reprod. Domest. Anim. 2004;39:438-441.

Makhafola MB, Lehloenya KC, Mphaphathi ML, Dinnyes A, Nedambale TL. The effect of breed on the survivability and motility rate of cryopreserved cock semen. S Afr J Anim Sci. 2009;39:242-5.

Margraf T, Karnopp AR, Rosso ND, Granato D. Comparison between folin-ciocalteu and prussian blue assays to estimate the total phenolic content of juices and teas using 96-well microplates. J Food Sci. 2015;80(11):2397-403. http://doi.org/10.1111/1750-3841.13077. PMid:26448565.

Marini PJ, Goodman BL. Semen characteristics as influenced by selection for divergent growth rate in Chickens. Poult Sci. 1969;48(3):859-65. http://doi.org/10.3382/ps.0480859. PMid:5389922.

Massip A, Leibo SP, Blesbois E. Cryobiology and the breeding of domestic animals. In: Benson E, Fuller B, Lane N. (eds.), Life in the Frozen State. London: Taylor and Francis Group; 2004. p. 371-92. http://doi.org/10.1201/9780203647073.ch12.

Morais MRPT, Velho ALMCS, Dantas SES, Fontenele-Neto JD. Morfofisiologia da reprodução das aves: desenvolvimento embrionário, anatomia e histologia do sistema reprodutor. Acta Vet Bras. 2012;6:165-76.

Moss SM, Moss DR, Arce SM, Lightner DV, Lotz JM. The role of selective breeding and biosecurity in the prevention of disease in penaeid shrimp aquaculture. J Invertebr Pathol. 2012;110(2):247-50. http://doi.org/10.1016/j.jip.2012.01.013. PMid:22434005.

Partyka A, Lukaszewicz E, Nizanski W. Effect of cryopreservation on sperm parameters, lipid peroxidation and antioxidant enzymes activity in fowl semen. Theriogenology. 2012;77(8):1497-504. http://doi.org/10.1016/j.theriogenology.2011.11.006. PMid:22225691.

Partyka A, Nizanski W. Advances in storage of poultry semen. Anim Reprod Sci. 2022;246:106921. http://doi.org/10.1016/j.anireprosci.2021.106921. PMid:34996657.

Partyka A, Nizanski W. Supplementation of avian semen extenders with antioxidants to improve semen quality - is it an effective strategy? Antioxidants. 2021;10(12):1927. http://doi.org/10.3390/antiox10121927. PMid:34943030.

Purdy PH, Song Y, Silversides FG, Blackburn HD. Evaluation of glycerol removal techniques, cryoprotectants, and insemination methods for cryopreserving rooster sperm with implications of regeneration of breed or line or both. Poult Sci. 2009;88(10):2184-91. http://doi.org/10.3382/ps.2008-00402. PMid:19762874.

Ribeiro PFA, Stringheta PC, Oliveira EB, Mendonça AC, Sant’ana HMP. Teor de vitamina C, β-caroteno e minerais em camu-camu cultivado em diferentes ambientes. Cienc Rural. 2016;46(3):567-72. http://doi.org/10.1590/0103-8478cr20150024.

Rodenas CEO, Murgas LDS, Maciel MP, Ferraz JM, Ribeiro MC, Bertechini AG, Freitas RTF, Fialho ET. Características seminais de galos alimentados com rações suplementadas com diferentes óleos e níveis de vitamina E. Cienc Agrotec. 2005;29(1):160-7. http://doi.org/10.1590/S1413-70542005000100020.

Rosenstrauch A, Egen AA, Friedländer M. Spermatozoa retention by Sertoli cells during the decline in fertility in aging roosters. Biol Reprod. 1994;50(1):129-36. http://doi.org/10.1095/biolreprod50.1.129. PMid:8312436.

Rostagno HS, Albino LFT, Hannas MI, Donzele JL, Sakomura NK, Costa FGP, Saraiva A, Teixeira ML, Rodrigues PB, Oliveira RF, Barreto SLT, Brito CO. Brazilian tables for poultry and swine: food composition and nutritional requirements. Viçosa: Federal University of Viçosa Press; 2017. [in Portuguese]

Rowell JG, Cooper DM. Some effects of diluting cock semen. Poult Sci. 1960;39(6):1381-9. http://doi.org/10.3382/ps.0391381.

Rufino JPF, Cruz FGG, Machado NJB, Brasil RJM, Pereira PAM, Farias EG. Incubation artificial processes associated with the application of different reproductive methods in matrices semi heavy. Rev Bras Saúde Prod Anim. 2014;15:765-73. http://doi.org/10.1590/S1519-99402014000300024.

Rutz F, Anciuti MA, Xavier EG, Roll VFB, Rossi P. Advances in physiology of reproduction in poultry. Rev Bras Reprod Anim. 2007;31:307-17.

Sexton TJ, Fewlass TA. A new poultry semen extender 2. Effect of the diluent components on the fertilizing capacity of chicken semen stored at 5°C. Poult Sci. 1977;57(1):277-84. http://doi.org/10.3382/ps.0570277. PMid:674015.

Sexton TJ. A new poultry semen extender 1. Effect of extension on the fertility of chicken semen. Poult Sci. 1977;56(5):1443-6. http://doi.org/10.3382/ps.0561443. PMid:605094.

Siari S, Mehri M, Sharafi M. Supplementation of Beltsville extender with quercetin improves the quality of frozen-thawed rooster semen. Br Poult Sci. 2021 PMid:34259575.

Silva RAJA, Batista AM, Arruda LCP, Souza HM, Nery IHAV, Gomes WA, Soares PC, Silva SV, Guerra MMP. Concentration of soybean lecithin affects short-term storage success of goat semen related with seminal plasma removal. Anim Reprod. 2019;16(4):895-901. http://doi.org/10.21451/1984-3143-AR2019-0012. PMid:32368269.

Silva SV, Guerra MMP. Cryopreservation effects on sperm cells and alternatives for crioinjury reduction. Rev Bras Reprod Anim. 2011;35:370-84.

Sonseeda P, Vongpralub T, Laopaiboon B. Effects of environmental factors, ages and breeds on semen characteristics in Thai indigenous chickens: a one-year study. Wetchasan Sattawaphaet. 2013;43(3):347-52. http://doi.org/10.56808/2985-1130.2490.

Souza FCA, Silva EP, Aguiar JPL. Vitamin characterization and volatile composition of camu-camu (Myrciaria dubia (HBK) McVaugh, Myrtaceae) at different maturation stages. Food Sci Technol (Campinas). 2021;41(4):961-6. http://doi.org/10.1590/fst.27120.

Surai PF, Wishart GJ. Poultry artificial insemination technology in the countries of the former USSR. Worlds Poult Sci J. 1996;52(1):27-43. http://doi.org/10.1079/WPS19960003.

Talebi A, Alimehr M, Alavi MH, Najafi G, Simaei N. Comparative study of semen traits and histomorphometric features of testes of broiler breeder males with different phenotypic traits. Vet Res Forum. 2018;9(1):1-6. PMid:29719657.

Taskin A, Ergun F, Karadavut U, Ergun D. Effect of different extenders on sperm motility and vitality in goose semen cryopreservation. Braz J Poult Sci. 2022;24(3):eRBCA-2020-1429. http://doi.org/10.1590/1806-9061-2020-1429.

van der Horst G, Maree L. SpermBlue®: A new universal stain for human and animal sperm which is also amenable to automated sperm morphology analysis. Biotech Histochem. 2010;84(6):299-308. http://doi.org/10.3109/10520290902984274. PMid:19488904.

Van Wambeke F. The effect of tonicity of storage media for fowl semen on the occurrence of neck-bending spermatozoa, fertility and hatchability. Br Poult Sci. 1977;18(2):163-8. http://doi.org/10.1080/00071667708416345. PMid:912470.

Wilcox FH. Changes in the pH of semen of the domestic cock as affected by temperature and frequency of collection. Poult Sci. 1958;37(2):444-9. http://doi.org/10.3382/ps.0370444.

Zapata SM, Dufour JP. Camu-camu Myrciaria dubia (HBK) McVaugh chemical composition of fruit. J Sci Food Agric. 1993;61(3):349-51. http://doi.org/10.1002/jsfa.2740610310.
 


Submitted date:
02/14/2024

Accepted date:
07/29/2024

66f6b7f9a95395060701ed13 animreprod Articles
Links & Downloads

Anim Reprod

Share this page
Page Sections