Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.1590/1984-3143-AR2024-0071
Animal Reproduction (AR)
Thematic Section: X International Symposium on Animal Biology of Reproduction (ISABR)

Successful transposition of sperm freezing, in vitro fertilization and artificial insemination methodologies in the Tadjik markhor species (Capra falconeri heptneri)

Yann Locatelli; Colin Vion; Nicolas Duffard; Nicolas Bon; Jérémy Bernard; Charly Binaud; Nina Trontti; Gérard Baril; Pascal Mermillod

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Abstract

Abstract: The objective of the present study was to transpose sperm freezing methodology from domestic goat to the Tadjik markhor (Capra falconeri heptneri) and to address the feasibility to develop IVP and artificial insemination using such frozen semen. Semen of different adult markhor males were successfully recovered by electro-ejaculation and were then frozen using caprine methodology. Frozen semen showed good survival rates at thawing and good fertility rates were assessed in heterologous in vitro fertilization system with goat oocytes. LOPU/IVF was applied for Tadjik markhor females allowing the first successful blastocyst production in vitro. In an applied program, we also transposed successfully intrauterine AI method with frozen/thawed semen to the Tadjik markhor.

Keywords

semen, artificial insemination, embryo production, LOPU

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Submitted date:
05/25/2024

Accepted date:
06/04/2024

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