Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.1590/1984-3143-AR2024-0074
Animal Reproduction (AR)
ORIGINAL ARTICLE

Effect of oxytocin and cloprostenol on the seminal parameters of red brocket deer (Mazama rufa) during the electroejaculation procedure

Cláudia Maria Herédias-Ribas; Yuki Tanaka; José Maurício Barbanti Duarte

Downloads: 0
Views: 47

Abstract

Successful implementation of conservation programs for endangered species requires biological material for use in reproductive biotechnologies. This enhances reproductive efficiency and helps increase the populations of critically endangered species. One way to facilitate the exchange of genetics between captive and free-ranging animals is through the creation of cryogenic banks that store cryopreserved gametes. In particular, semen cryopreservation allows for this exchange to occur. We evaluated whether the use of exogenous hormones (such as oxytocin and prostaglandin) prior to electroejaculation increases seminal volume, sperm concentration, and the number of doses produced in the red brocket deer (Mazama rufa). We also evaluate whether seminal parameters vary over the three stimulation cycles of the same electroejaculation procedure. The treatments did not affect ejaculate volume (p = 0.402), the number of sperm cells in the ejaculates (p = 0.926), total doses produced (p = 0.684), sperm mass movement (p = 0.229), sperm cell concentration (p = 0.106), and acrosome integrity (p = 0.210). The use of hormones has potential in reducing the need for stressful stimuli in electroejaculation, but the choice of hormones must take into account their effects on semen quality.

Keywords

Cervidae, hormonal stimulation, red brocket deer, reproductive biotechnologies, semen collection

References

Abril‐Sánchez S, Freitas‐de‐Melo A, Damián JP, Giriboni J, Villagrá‐García A, Ungerfeld R. Ejaculation does not contribute to the stress response to electroejaculation in sheep. Reprod Domest Anim. 2017;52(3):403-8. http://doi.org/10.1111/rda.12922. PMid:28120512.

Assinder SJ, Carey M, Parkinson T, Nicholson HD. Oxytocin and vasopressin expression in the ovine testis and epididymis: changes with the onset of spermatogenesis. Biol Reprod. 2000;63(2):448-56. http://doi.org/10.1095/biolreprod63.2.448. PMid:10906049.

Bainbridge DRJ, Jabbour HN. Potential of assisted breeding techniques for the conservation of endangered mammalian species in captivity: a review. Vet Rec. 1998;143(6):159-68. http://doi.org/10.1136/vr.143.6.159. PMid:9746946.

Berndtson WE, Igboeli G. Spermatogenesis, sperm output and seminal quality of Holstein bulls electroejaculated after administration of oxytocin. J Reprod Fertil. 1988;82(2):467-75. http://doi.org/10.1530/jrf.0.0820467. PMid:3361481.

Colégio Brasileiro de Reprodução Animal – CBRA. Manual para exame andrológico e avaliação de sêmen animal. 3. ed. Belo Horizonte: CBRA; 2013.

Cornwell J, Koonce K, Kreider J. Effect of prostaglandin F2α on seminal characteristics of the stallion. Anim Sci J. 1974;38:226.

Cursino MS, Salviano MB, Abril VV, Zanetti EDS, Duarte JMB. The role of chromosome variation in the speciation of the red brocket deer complex: the study of reproductive isolation in females. BMC Evol Biol. 2014;14(1):40. http://doi.org/10.1186/1471-2148-14-40. PMid:24593190.

Duarte JMB, Garcia JM. Reprodução assistida em Cervidae brasileiros. Rev Bras Reprod Anim. 1995;19(1-2):111-21.

Duarte JMB, Reis ML. Plano de ação nacional para a conservação dos cervídeos ameaçados de extinção. Brasília: Instituto Chico Mendes de Conservação da Biodiversidade; 2012.

Duarte JMB. Captive management. In: Duarte JMB, González S, editors. Neotropitcal cerviology: biology and medicine of Latin American deer. Jaboticabal: Funep/IUCN; 2010. p. 240-7

Ellis LC, Farr GCH, Tesi RJ. Contractility of seminiferous tubules as related to sperm transport in the male. Arch Androl. 1981;6(4):283-94. http://doi.org/10.3109/01485018108987539. PMid:6113819.

Favoretto SM, Zanetti ES, Duarte JMB. Cryopreservation of red brocket deer semen (Mazama americana): comparison between three extenders. J Zoo Wildl Med. 2012;43(4):820-7. http://doi.org/10.1638/2011-0195R1.1. PMid:23272349.

Fernandes DAM, Souza CV, Balaro MFA, Santos JDR, Santos VMB, Costa MMCP, Carvalho ABS, Rodrigues ALR, Ungerfeld R, Brandão FZ. Response of rams to electroejaculation following the administration of oxytocin and cloprostenol with or without GnRH. Theriogenology. 2021;173:32-6. http://doi.org/10.1016/j.theriogenology.2021.06.019. PMid:34265698.

Frankham R. Genetic adaptation to captivity in species conservation programs. Mol Ecol. 2008;17(1):325-33. http://doi.org/10.1111/j.1365-294X.2007.03399.x. PMid:18173504.

Fuchs AR, Husslein P, Fuchs F. Oxytocin and the initiation of human parturition: II. Stimulation of prostaglandin production in human decidua by oxytocin. Am J Obstet Gynecol. 1981;141(5):694-7. http://doi.org/10.1016/S0002-9378(15)33313-5. PMid:7315896.

Garner DL, Hafez ESE. Espermatozoides e plasma seminal. In: Hafez ESE, Hafez B, editors. Reprodução animal. Barueri: Manole; 2004. p. 97-110.

Hess M. The effects of prostaglandin-F2α, oxytocin and gonadotropin releasing hormone on ejaculate characteristics in the dog [dissertation]. Blacksburg: Virginia Polytechnic Institute and State University; 2002.

Ibrahim M. Influence of oxytocin and prostaglandin on semen characteristics and process of ejaculation in buffalo bulls. Acta Vet Hung. 1988;82(1-2):3-10. PMid:3202047.

Kelly RW. Prostaglandin synthesis in the male and female reproductive tract. Reprod. 1981;62(1):293-304. http://doi.org/10.1530/jrf.0.0620293. PMid:7230132.

Knight TW, Lindsay DR. Short and long-term effects of oxytocin on quality and quantity of semen from rams. J Reprod Fertil. 1970;21(3):523-9. http://doi.org/10.1530/jrf.0.0210523. PMid:5442318.

Knight TW. A qualitative study of the factors affecting the contractions of the epidicymis and ductus deferens of the ram. J Reprod Fertil. 1974;40(1):19-29. http://doi.org/10.1530/jrf.0.0400019. PMid:4411718.

Mosure WL, Meyer RA, Gudmundson J, Barth AD. Evaluation of possible methods to reduce pain associated with electroejaculation in bulls. Can Vet J. 1998;39(8):504-6. PMid:9711391.

Nicholson HD, Parkinson TJ, Lapwood KR. Effects of oxytocin and vasopressin on sperm transport from the cauda epididymis in sheep. J Reprod Fertil. 1999;117(2):299-305. http://doi.org/10.1530/jrf.0.1170299. PMid:10690197.

Niemi M, Kormano M. Histochemical demonstration of a C-esterase activity in the seminiferous tubules of the rat testis. Reproduction. 1965;10(1):49-54. http://doi.org/10.1530/jrf.0.0100049. PMid:14337814.

Palmer CW, Amundson SD, Brito LFC, Waldner CL, Barth AD. Use of oxytocin and cloprostenol to facilitate semen collection by electroejaculation or transrectal massage in bulls. Anim Reprod Sci. 2004;80(3-4):213-23. http://doi.org/10.1016/j.anireprosci.2003.07.003. PMid:15036498.

Peres PH, Luduvério DJ, Bernegossi AM, Galindo DJ, Nascimento GB, Oliveira ML, Sandoval EDO, Vozdova M, Kubickova S, Cernohorska H, Duarte JMB. Revalidation of Mazama rufa (Illiger 1815) (Artiodactyla: Cervidae) as a distinct species out of the complex Mazama americana (Erxleben 1777). Front Genet. 2021;12:742870. http://doi.org/10.3389/fgene.2021.742870. PMid:34970296.

Phaneuf S, Europe-Finner GN, Varney M, Mackenzie IZ, Watson SP, Bernal AL. Oxytocin-stimulated phosphoinositide hydrolysis in human myometrial cells: involvement of pertussis toxin-sensitive and-insensitive G-proteins. J Endocrinol. 1993;136(3):497-509. http://doi.org/10.1677/joe.0.1360497. PMid:8386215.

Pope CE, Zhang YZ, Dresser BL. A simple staining method for evaluating acrosomal status of cat spermatozoa. J Zoo Wildl Med. 1991;22:87-95.

Pukazhenthi BS, Wildt DE. Which reproductive technologies are most relevant to studying, managing and conserving wildlife? Reprod Fertil Dev. 2004;16(1-2):33-46. http://doi.org/10.1071/RD03076. PMid:14972101.

R Development Core Team. R: a language and environment for statistical computing. Vienna: R Foundation for Statistical Computing; 2013.

Rola LD, Buzanskas ME, Melo LM, Chaves MS, Freitas VJF, Duarte JMB. Assisted reproductive technology in neotropical deer: a model approach to preserving genetic diversity. Animals. 2021;11(7):1961. http://doi.org/10.3390/ani11071961. PMid:34209061.

Rola LD, Zanetti ES, Duarte JMB. Evaluation of semen characteristics of the species Mazama americana in captivity. Anim Prod Sci. 2013;53(5):472-7. http://doi.org/10.1071/AN12247.

Salviano MB, Cursino MS, Zanetti EDS, Abril VV, Duarte JMB. Intraspecific chromosome polymorphisms can lead to reproductive isolation and speciation: an example in red brocket deer (Mazama americana). Biol Reprod. 2017;96(6):1279-87. http://doi.org/10.1093/biolre/iox041. PMid:28486644.

Silva AR, Lima GL, Peixoto GCX, Souza ALP. Cryopreservation in mammalian conservation biology: current applications and potential utility. Res Rep Bio Stud. 2015;4:1-8. http://doi.org/10.2147/RRBS.S54294.

Silva AR, Morato RG, Silva LDM. The potential for gamete recovery from non-domestic canids and felids. Anim Reprod Sci. 2004;81(1-2):159-75. http://doi.org/10.1016/j.anireprosci.2003.10.001. PMid:14749057.

Silva AR, Souza ALP, Santos EAAD, Lima GL, Peixoto GCX, Souza PC, Castelo TS. Formação de Bancos de Germoplasma e sua contribuição para a conservação de espécies silvestres no Brasil. Ciênc Anim. 2012;22(1):219-34.

Spindler RE, Wildt DE. Male reproduction: assesment, management, assisted breeding, and fertility control. In: Kleiman DG, Thompson KV, Baer CK, editors. Wild mammals in captivity: principles and techniques for zoo management. Chicago: University of Chicago Press; 2010. p. 429-46.

Stafford KJ, Spoorenberg J, West DM, Vermunt JJ, Petrie N, Lawoko CRO. The effect of electro-ejaculation on aversive behaviour and plasma cortisol concentration in rams. N Z Vet J. 1996;44(3):95-8. http://doi.org/10.1080/00480169.1996.35943. PMid:16031903.

Thackare H, Nicholson HD, Whittington K. Oxytocin: its role in male reproduction and new potential therapeutic uses. Hum Reprod. 2006;12(4):437-48. http://doi.org/10.1093/humupd/dmk002. PMid:16436468.

Ungerfeld R, Abril-Sánchez S, Toledano-Díaz A, Beracochea F, Castaño C, Giriboni J, Santiaho-Moreno J. Oxytocin administration before sperm collection by transrectal ultrasonic-guided massage of the accessory sex glands in mouflons and bucks. Anim Reprod Sci. 2016;173:13-7. http://doi.org/10.1016/j.anireprosci.2016.08.002. PMid:27530370.

Ungerfeld R, Casuriaga D, Giriboni J, Freitas-de-Melo A, Silveira P, Brandão FZ. Administration of cloprostenol and oxytocin before electroejaculation in goat bucks reduces the needed amount of electrical stimulation without affecting seminal quality. Theriogenology. 2018;107:1-5. http://doi.org/10.1016/j.theriogenology.2017.10.034. PMid:29120706.

Voglmayr J. Prostaglandin F2α concentration in genital tract secretions of dairy bulls. Prostaglandins. 1973;4(5):673-8. http://doi.org/10.1016/S0090-6980(73)80049-8. PMid:4360951.

Whittington K, Assinder SJ, Parkinson T, Lapwood KR, Nicholson HD. Function and localization of oxytocin receptors in the reproductive tissue of rams. Reproduction. 2001;122(2):317-25. http://doi.org/10.1530/rep.0.1220317. PMid:11467983.

Worley RTS, Nicholson HD, Pickering BT. Testicular oxytocin: an initiator of seminiferous tubule movement. In: Courtens JL, editor. Recent progress in cellular endocrinology of the testis. Paris: Inserm Paris; 1956. p. 205-12.
 


Submitted date:
05/29/2024

Accepted date:
02/11/2025

67ddb8bca953953f4569af73 animreprod Articles
Links & Downloads

Anim Reprod

Share this page
Page Sections