Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.1590/1984-3143-AR2024-0127
Animal Reproduction (AR)
ORIGINAL ARTICLE

Quantification of polyoocyte follicles (POFs) in domestic cats

Alexandra Lays Petri; Mariana Valentini Casagrande; Adalgiza Pinto Neto; Jonatas Cattelam; Camila Keterine Gorzelanski Trenkel; Jacqueline Zanella; Leonardo Gruchouskei

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Abstract

Ovarian follicles usually involve only one oocyte. However, the existence of follicles with more than one oocyte has been described in different species. Therefore, this study evaluated the occurrence and number of Polyoocyte Follicles (POFs) in the ovaries of cats undergoing the ovariohysterectomy technique. For this purpose, ovaries from 33 cats were collected and submitted to histological preparation and analysis. The slides were evaluated under a microscope by two evaluators, who quantified the presence of follicles containing one or more oocytes and classified the follicular stage. Monoocyte follicles (MOFs) were found in all ovaries. POFs were observed at similar frequencies in both the right and left ovaries. The frequency of POFs with two oocytes was 79.03% in the right ovaries and 75.75% in the left ovaries. Follicles with three oocytes were found in 16.12% of the right ovaries and 27.27% of the left ovaries. The mean number of follicles with zero, one, two, three or more oocytes was similar between the ovaries, being 6.58, 310.5, 3.25, and 0.29 in the right ovary and 6.25, 312.7, 4.25, and 0.41 in the left ovary (p-values ​​of 0.7569, 0.9654, 0.4785, and 0.5015, respectively). POFs containing from two to five oocytes were identified in different stages of development. POFs at all stages of development were found in both ovaries, and the occurrence of these structures was influenced by the age, number of pregnancies, number of estrus, and weight of the cats studied.

Keywords

feline, ovary, folliculogenesis, oocyte

References

Araújo VR, Celestino JJ H, Chaves RN. Aspectos qualitativos e quantitativos da atresia durante a foliculogênese em mamíferos. Ciênc Anim. 2021;31(3):112-28.

Astudillo I, Aspee K, Palomino J, Peralta OA, Parraguez VH, de los Reyes M. Meiotic development of canine oocytes from poly-ovular and mono-ovular follicles after in vitro maturation. Animals. 2023;13(4):648. http://doi.org/10.3390/ani13040648. PMid:36830434.

Bristol-Gould S, Woodruff TK. Folliculogenesis in the domestic cat (Felis catus). Theriogenology. 2006;66(1):5-13. http://doi.org/10.1016/j.theriogenology.2006.03.019. PMid:16620931.

Cardoso CFR. Desenvolvimento folicular ao longo do ciclo éstrico na cadela e gata [dissertação]. Lisboa: Universidade Lusófona de Humanidades e Tecnologias; 2017.

Carrijo OA Jr, Marinho APS, Campos AA, Amorim CA, Báo SN, Lucci CM. Morphometry, estimation and ultrastructure of ovarian preantral follicle population in queens. Cells Tissues Organs. 2010;191(2):152-60. http://doi.org/10.1159/000225935. PMid:19546511.

Dederer PH. Polyovular follicles in the cat. Anat Rec. 1934;60(4):391-7. http://doi.org/10.1002/ar.1090600404.

Gougeon A. Frequent occurrence of multiovular follicles and multinuclear oocytes in the adult human ovary. Fertil Steril. 1981;35(4):417-22. http://doi.org/10.1016/S0015-0282(16)45436-1. PMid:7215567.

Hafez B, Hafez ESE. Anatomia da reprodução feminina. In: Hafez B, Hafez ESE, editors. Reprodução animal. 7. ed. Barueri: Manole; 2004.

Hartman CG. Polynuclear ova and polyovular follicles in the opossum and other mammals, with special reference to the problem of fecundity. Am J Anat. 1926;37(1):1-51. http://doi.org/10.1002/aja.1000370102.

Jefferson WN, Couse JF, Padilla-Banks E, Korach KS, Newbold RR. Neonatal exposure to genistein induces estrogen receptor (ER) α expression and multioocyte follicles in the maturing mouse ovary: evidence for ERβ-mediated and nonestrogenic actions. Biol Reprod. 2002;67(4):1285-96. http://doi.org/10.1095/biolreprod67.4.1285. PMid:12297547.

Jewgenow K, Goritz F. The recovery of preantral follicles from ovaries of domestic cats and their characterisation before and after culture. Anim Reprod Sci. 1995;39(4):285-97. http://doi.org/10.1016/0378-4320(95)01397-I.

Johnson J, Canning J, Kaneko T, Pru JK, Tilly JL. Germline stem cells and follicular renewal in the postnatal mammalian ovary. Nature. 2004;428(6979):145-50. http://doi.org/10.1038/nature02316. PMid:15014492.

Junqueira LC, Carneiro J. Métodos de estudo em histologia. In: Junqueira LC, Carneiro J, editors. Histologia básica. 12. ed. Rio de Janeiro: Guanabara Koogan; 2013.

Lunardon NT, Silva-Santos KC, Justino RC, Dessunti GT, Seneda MM, Martins MIM. Population estimate of the preantral follicles and frequency of multioocyte follicles in pre-pubertal and adult bitches. Theriogenology. 2015;83(6):1015-20. http://doi.org/10.1016/j.theriogenology.2014.12.002. PMid:25572648.

Miclăuş V, Groza I, Oana L. Domestic cat (Felis catus) polyovular follicles. Bull USAMV-CN. 2007;64:1-6.

Monteiro CMR, de Koivisto MB, Silva A. Perfil histológico do útero e ovários de gatas submetidas à ovariosalpingohisterectomia. Ars Vet. 2006;22(3):236-41.

Oliveira RL, Silva CB, Silva EO, Gerez JR, Santos MM, Sarapião FD, Sartori L, Bracarense APFRL, Seneda MM. Proliferative activity of multi-oocyte follicles in sheep ovaries. Small Rumin Res. 2017;146:58-60. http://doi.org/10.1016/j.smallrumres.2016.12.004.

Payan-Carreira R, Pires MA. Multioocyte follicles in domestic dogs: a survey of frequency of occurrence. Theriogenology. 2008;69(8):977-82. http://doi.org/10.1016/j.theriogenology.2008.01.013. PMid:18358525.

Shehata R. Polyovular Graafian follicles in a newborn kitten with a study of polyovuli in the cat. Acta Anat. 1974;89(1):21-30. http://doi.org/10.1159/000144266. PMid:4136167.

Silva-Santos KC, Seneda MM. Multioocyte follicles in adult mammalian ovaries. Anim Reprod. 2011;8:58-67. http://doi.org/10.1590/1678-4162-12385.

Stankiewicz T, Błaszczyk B, Udała J. A study on the occurrence of polyovular follicles in porcine ovaries with particular reference to intrafollicular hormone concentrations, quality of oocytes and their in vitro fertilization. Anat Histol Embryol. 2009;38(3):233-9. http://doi.org/10.1111/j.1439-0264.2009.00929.x. PMid:19469770.

Telfer E, Gosden RG. A quantitative cytological study of polyovular follicles in mammalian ovaries with particular reference to the domestic bitch (Canis familiaris). J Reprod Fertil. 1987;81(1):137-47. http://doi.org/10.1530/jrf.0.0810137. PMid:3668945.

Uchikura K, Nagano M, Hishinuma M. Evaluation of follicular development and oocyte quality in pre-pubertal cats. Reprod Domest Anim. 2010;45(6):e405-11. http://doi.org/10.1111/j.1439-0531.2010.01590.x. PMid:20210881.

Zoppei AP, Pinto A No, Cattelam J, Martinez AC, Trenkel CKG, Steffens R, Gruchouskei L. Quantification of multi-oocyte follicles in ovaries of bitches. Arq Bras Med Vet Zootec. 2021;73(6):1269-77. http://doi.org/10.1590/1678-4162-12385.
 


Submitted date:
11/04/2024

Accepted date:
06/04/2025

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