Insights into early embryonic development - a personal assessment based on 30 years of experience
Urban Besenfelder
Abstract
Keywords
References
Aigner B, Besenfelder U, Mueller M, Brem G. Tyrosinase gene variants in different rabbit strains. Mamm Genome. 2000;11(8):700-2.
Anel L, Alvarez M, Martinez-Pastor F, Garcia-Macias V, Anel E, de Paz P. Improvement strategies in ovine artificial insemination. Reprod Domest Anim. 2006;41(2, Suppl 2):30-42.
Besenfelder U, Brem G. Laparoscopic embryo transfer in rabbits. J Reprod Fertil. 1993;99(1):53-6.
Besenfelder U, Brem G. Tubal transfer of bovine embryos: a simple endoscopic method reducing long-term exposure of in vitro produced embryos. Theriogenology. 1998;50(5):739-45.
Besenfelder U, Haas C, Brem G. Reproduction technology and gene transfer in rabbits. World Rabbit Sci. 2000;8(1):37-59.
Besenfelder U, Havlicek V, Kuzmany A, Brem G. Endoscopic approaches to manage in vitro and in vivo embryo development: use of the bovine oviduct. Theriogenology. 2010;73(6):768-76.
Besenfelder U, Havlicek V, Mösslacher G, Brem G. Collection of tubal stage bovine embryos by means of endoscopy. A technique report. Theriogenology. 2001;55(3):837-45.
Besenfelder U, Havlicek V. The interaction between the environment and embryo development in assisted reproduction. Anim Reprod. 2023;20(2):e20230034.
Besenfelder U, Mödl J, Müller M, Brem G. Endoscopic embryo collection and embryo transfer into the oviduct and the uterus of pigs. Theriogenology. 1997;47(5):1051-60.
Besenfelder U, Strouhal C, Brem G. A method for endoscopic embryo collection and transfer in the rabbit. Zentralbl Veterinärmed A. 1998a;45(9):577-9.
Besenfelder U, Muller M, Brem G. Transgenics and modern reproductive technologies. In: Rothschild M, F, Ruvinsky A, editors. The genetics of the pig. Wallingford: CAB International; 1998b. pp. 345-374.
Besenfelder U, Solti L, Seregi J, Brem G. Influence of beta-carotene on fertility in rabbits when using embryo transfer programs. Theriogenology. 1993;39(5):1093-109.
Besenfelder U, Solti L, Seregi J, Müller M, Brem G. Different roles for B-carotene and vitamin A in the reproduction on rabbits. Theriogenology. 1996;45(8):1583-91.
Besenfelder U, Zinovieva N, Dietrich E, Sohnrey B, Holtz W, Brem G. Tubal transfer of goat embryos using endoscopy. Vet Rec. 1994;135(20):480-1.
Bolet G, Brun JM, Monnerot M, Abeni F, Arnal C, Arnold J, Bell D, Bergoglio G, Besenfelder U, Bosze S, Boucher S, Chanteloup N, Ducourouble MC, Durand-Tardif M, Esteves PJ, Ferrand N, Gautier A, Haas C, Hewitt G, Jehl N, Joly T, Koehl PF, Laube T, Lechevestrier S, Lopez M, Masoero G, Menigoz JJ, Piccinin R, Queney G, Saleil G, Surridge A, Van Der Loo W, Vicente JS, Viudes De Castro MP, Virag JS, Zimmermann JM. Evaluation and conservation of European rabbit (oryctolagus cuniculus) genetic resources. First results and inferences. World Rabbit Sci. 2000;8(1):281-315.
Brüssow KP, Torner H, Kanitz W, Rátky J. In vitro technologies related to pig embryo transfer. Reprod Nutr Dev. 2000;40(5):469-80.
Cameron RD, Durack M, Fogarty R, Putra DK, McVeigh J. Practical experience with commercial embryo transfer in pigs. Aust Vet J. 1989;66(10):314-8.
Carter F, Rings F, Mamo S, Holker M, Kuzmany A, Besenfelder U, Havlicek V, Mehta JP, Tesfaye D, Schellander K, Lonergan P. Effect of elevated circulating progesterone concentration on bovine blastocyst development and global transcriptome following endoscopic transfer of in vitro produced embryos to the bovine oviduct. Biol Reprod. 2010;83(5):707-19.
Ehling C, Wirth P, Schindler L, Hadeler KG, Döpke HH, Lemme E, Herrmann D, Niemann H. Laparoscopical intrauterine insemination with different doses of fresh, conserved, and frozen semen for the production of ovine zygotes. Theriogenology. 2003;60(4):777-87.
Fayrer-Hosken RA, Younis AI, Brackett BG, McBride CE, Harper KM, Keefer CL, Cabaniss DC. Laparoscopic oviductal transfer of in vitro matured and in vitro fertilized bovine oocytes. Theriogenology. 1989;32(3):413-20.
Forcada F, Sánchez-Prieto L, Casao A, Palacín I, Cebrián-Pérez JA, Muiño-Blanco T, Abecia JA. Use of laparoscopic intrauterine insemination associated with a simplified superovulation treatment for
Gad A, Besenfelder U, Rings F, Ghanem N, Salilew-Wondim D, Hossain MM, Tesfaye D, Lonergan P, Becker A, Cinar U, Schellander K, Havlicek V, Hölker M. Effect of reproductive tract environment following controlled ovarian hyperstimulation treatment on embryo development and global transcriptome profile of blastocysts: implications for animal breeding and human assisted reproduction. Hum Reprod. 2011;26(7):1693-707.
Gad A, Hoelker M, Besenfelder U, Havlicek V, Cinar U, Rings F, Held E, Dufort I, Sirard MA, Schellander K, Tesfaye D. Molecular mechanisms and pathways involved in bovine embryonic genome activation and their regulation by alternative in vivo and in vitro culture conditions. Biol Reprod. 2012;87(4):100.
Grosse-Hovest L, Müller S, Minoia R, Wolf E, Zakhartchenko V, Wenigerkind H, Lassnig C, Besenfelder U, Müller M, Lytton SD, Jung G, Brem G. Cloned transgenic farm animals produce a bispecific antibody for T cell-mediated tumor cell killing. Proc Natl Acad Sci USA. 2004;101(18):6858-63.
Havlicek V, Autz AK, Hoelker M, Blaschka C, Besenfelder U. Collection of embryos and fluid from the bovine oviduct. Anim Reprod. 2022;19(2 spe):e22193.
Havlicek V, Brem G, Besenfelder U. (2018). Endoscopy in cattle reproduction. In: Niemann H, Wrenzycki C, editors. Animal Biotechnology 1. Cham: Springer. p. 193-207.
Havlicek V, Kuzmany A, Cseh S, Brem G, Besenfelder U. The effect of long-term in vivo culture in bovine oviduct and uterus on the development and cryo-tolerance of in vitro produced bovine embryos. Reprod Domest Anim. 2010;45(5):832-7. PMid:19416481.
Hazeleger W, Kemp B. State of the art in pig embryo transfer. Theriogenology. 1999;51(1):81-90.
Kühholzer B, Müller S, Treuer A, Seregi J, Besenfelder U, Brem G. Repeated endoscopic ovum pick-up in hormonally untreated ewes: a new technique. Theriogenology. 1997a;48(4):545-50.
Kühholzer B, Besenfelder U, Müller S, Reichenbach HD, Brem G. Laparoscopic insemination of seasonally anoestrous ewes by a simplified method under field conditions. Reprod Domest Anim. 1997b;32(6):309-12.
Kühholzer B, Müller S, Besenfelder U, Prokofiev MI, Ernst LK, Brem G. Laparoscopic recovery of pronuclear-stage goat embryos. Vet Rec. 1998a;142(2):40-2.
Kühholzer B, Müller S, Prokofiev MI, Ernst LK, Besenfelder U, Brem G. Laparoscopic techniques for the recovery and transfer of microinjected goat zygotes. Theriogenology. 1998b;49(1):245.
Laurincík J, Pícha J, Píchová D, Oberfranc M. Timing of laparoscopic aspiration of preovulatory oocytes in heifers. Theriogenology. 1991;35(2):415-23.
Lymberopoulos AG, Amiridis GS, Kühholzer B, Besenfelder U, Christodoulou V, Vainas E, Brem G. Fertilization and embryo recovery rates in superovulated chios ewes after laparoscopic intrauterine insemination. Theriogenology. 2001;55(9):1855-62.
Maillo V, Rizos D, Besenfelder U, Havlicek V, Kelly AK, Garrett M, Lonergan P. Influence of lactation on metabolic characteristics and embryo development in postpartum Holstein dairy cows. J Dairy Sci. 2012;95(7):3865-76.
Mertens E, Besenfelder U, Gilles M, Hölker M, Rings F, Havlicek V, Schellander K, Herrler A. Influence of in vitro culture of bovine embryos on the structure of the Zona pellucida. Reprod Fertil Dev. 2007;19(1):211-2.
Mertens E, Gilles M, Rings F, Hölker M, Schellander K, Herrler A. Influence of in vitro culture of bovine embryos on the structure of the zona pellucid. Reprod Domest Anim. 2006;41(1):23.
Miskel D, Beunink L, Poirier M, Havlicek V, Rings F, Schellander K, Besenfelder U, Hölker M. CRISPR/Cas9 gene editing of in vivo-fertilized bovine embryos via endoscopic oviductal flushing and electroporation of zygotes. Reprod Fertil Dev. 2019;32(2):193.
Neubrand L, Pothmann H, Havlicek V, Besenfelder U, Aurich C, Drillich M, Gabler C, Wagener K. Oestrous cycle dependent alterations in the mRNA abundance of pro-inflammatory factors in the bovine oviductal epithelium in vivo. Reprod Domest Anim. 2021;56(1):45. PMid:34647367.
Nowshari MA, Besenfelder U, Moeszlacher G, Brem G. Nuclear transfer with fetal adult and follicular cells in rabbits. Theriogenology. 2002;57:439.
Papp SM, Fröhlich T, Radefeld K, Havlicek V, Kösters M, Yu H, Mayrhofer C, Brem G, Arnold GJ, Besenfelder U. A novel approach to study the bovine oviductal fluid proteome using transvaginal endoscopy. Theriogenology. 2019;132:53-61.
Pothmann H, Ibrahim M, Besenfelder U, Havlicek V, Drillich M, Gabler C. Hyperketonemia affects mRNA expression of selected pro-inflammatory factors and metabolic enzymes in the endometrium and oviduct of postpartum dairy cows. Reprod Domest Anim. 2022;57(3):18.
Radefeld K, Papp S, Havlicek V, Morrell JM, Brem G, Besenfelder U. Endoscopy-mediated intratubal insemination in the cow - Development of a novel minimally invasive AI technique. Theriogenology. 2018;115:117-23.
Rátky J, Brüssow KP. Successful embryo recovery from swine by a minimal invasive technique. Acta Vet Hung. 1995;43(4):371-5. PMid:8882735.
Reichenbach HD, Wiebke NH, Besenfelder UH, Modl J, Brem G. Transvaginal laparoscopic guided aspiration of bovine follicular oocytes: preliminary results. Theriogenology. 1993;39(1):295.
Rizos D, Carter F, Besenfelder U, Havlicek V, Lonergan P. Contribution of the female reproductive tract to low fertility in postpartum lactating dairy cows. J Dairy Sci. 2010;93(3):1022-9.
Rodríguez-Alonso B, Sánchez JM, Hamdi M, McDonald M, Havlicek V, Besenfelder U, Lonergan P, Rizos D. Asynchrony between the early embryo and the reproductive tract affects subsequent embryo development in cattle. Reprod Fertil Dev. 2020;32(6):564-71.
Salilew-Wondim D, Fournier E, Hoelker M, Saeed-Zidane M, Tholen E, Looft C, Neuhoff C, Besenfelder U, Havlicek V, Rings F, Gagné D, Sirard MA, Robert C, Shojaei Saadi HA, Gad A, Schellander K, Tesfaye D. Genome-Wide DNA Methylation Patterns of Bovine Blastocysts Developed In Vivo from Embryos Completed Different Stages of Development In Vitro. PLoS One. 2015;10(11):e0140467.
Salilew-Wondim D, Saeed-Zidane M, Hoelker M, Gebremedhn S, Poirier M, Pandey HO, Tholen E, Neuhoff C, Held E, Besenfelder U, Havlicek V, Rings F, Fournier E, Gagné D, Sirard MA, Robert C, Gad A, Schellander K, Tesfaye D. Genome-wide DNA methylation patterns of bovine blastocysts derived from in vivo embryos subjected to in vitro culture before, during or after embryonic genome activation. BMC Genomics. 2018;19(1):424.
Shackell GH, Kyle B, Littlejohn RP. Factors influencing the success of large scale artificial insemination programme in sheep. Proc NZ Soc Anim Prod. 1990;50:427-30.
Snyder DA, Dukelow WR. Laparoscopic studies of ovulation, pregnancy diagnosis, and follicle aspiration in sheep. Theriogenology. 1974;2(6):143-8.
Stangl M, Kühholzer B, Besenfelder U, Brem G. Repeated endoscopic ovum pick-up in sheep. Theriogenology. 1999;52(4):709-16.
Tšuiko O, Catteeuw M, Zamani Esteki M, Destouni A, Bogado Pascottini O, Besenfelder U, Havlicek V, Smits K, Kurg A, Salumets A, D’Hooghe T, Voet T, Van Soom A, Robert Vermeesch J. Genome stability of bovine in vivo-conceived cleavage-stage embryos is higher compared to in vitro-produced embryos. Hum Reprod. 2017;32(11):2348-57.
Vainas E, Papakostaki D, Christodoulou V, Besenfelder U, Amiridis GS, Kuehholzer B, Samartzi F, Brem G. Exploitation of embryos collected from Maedi-Visna seropositive ewes during eradication programs. Small Rumin Res. 2006;62(1-2):129-34.
Wetscher F, Havlicek V, Huber T, Gilles M, Tesfaye D, Griese J, Wimmers K, Schellander K, Müller M, Brem G, Besenfelder U. Intrafallopian transfer of gametes and early stage embryos for in vivo culture in cattle. Theriogenology. 2005a;64(1):30-40.
Wetscher F, Havlicek V, Huber T, Müller M, Brem G, Besenfelder U. Effect of morphological properties of transferred embryonic stages on tubal migration Implications for in vivo culture in the bovine oviduct. Theriogenology. 2005b;64(1):41-8.
Submitted date:
02/25/2025
Accepted date:
04/17/2025