In vivo versus in vitro embryo production in small ruminants: strengths, limitations, and practical outcomes
Joanna Maria Gonçalves Souza-Fabjan; Ana Paula Silva Cupello; Nicole Oliveira Rocha; Matheus Silva Admiral Gomes; Marie Saint-Dizier; Ribrio Ivan Tavares Pereira Batista
Abstract
Keywords
References
Agarwal A, Said TM, Bedaiwy MA, Banerjee J, Alvarez JG. Oxidative stress in assisted reproductive techniques setting. Fertil Steril. 2006;86(3):503-12.
Alegretti JR, Rocha AMD, Nogueira-de-Souza NC, Kato N, Barros BC, Motta ELA, Serafini PC, Takayama S, Smith GD. Controlled dynamic microfluidic culture of murine, bovine, and human embryos improves development: proof-of-concept studies. Cells. 2024;13(24):2080.
Alm H, Torner H, Löhrke B, Viergutz T, Kanitz W. BCB staining as a tool for oocyte selection in farm animals: relationship with mitochondrial activity and embryo development. Theriogenology. 2020;150:119-28.
Amiridis GS, Cseh S. Assisted reproductive technologies in the reproductive management of small ruminants. Anim Reprod Sci. 2012;130(3-4):152-61.
Anguita B, Jimenez-Macedo A, Izquierdo D, Mogas T, Paramio MT. Effect of oocyte diameter on meiotic competence, embryo development, p34(cdc2) expression and MPF activity in prepubertal goat oocytes. Theriogenology. 2007;67(3):526-36.
Anguita B, Paramio MT, Jimenez-Macedo AR, Morato R, Mogas T, Izquierdo D. Total RNA and protein content, cyclin B1 expression and developmental competence of prepubertal goat oocytes. Anim Reprod Sci. 2008;103(3-4):290-303.
Anzalone DA, Palazzese L, Czernik M, Sabatucci A, Valbonetti L, Capra E, Loi P. Controlled spermatozoa-oocyte interaction improves embryo quality in sheep. Sci Rep. 2021;11(1):22629.
Arrais AM, Mello MRB, Vergani GB, Figueira LM, Esteves SN, Pereira VSA, Bartlewski PM, Oliveira MEF, Souza-Fabjan JMG, Fonseca JF. Nonsurgical embryo recovery from estrus-synchronized or superovulated Morada Nova ewes: a feasible strategy for sheep embryo banking. Biopreserv Biobank. 2021;19(5):360-8.
Aybazov M, Selionova M, Trukhachev V, Malorodov V, Yuldashbayev Y, Easa AA. Embryo production and transplantation in non-breeding season of meat sheep breeds by stimulating superovulation with different follicle-stimulating hormone preparations. Reprod Domest Anim. 2023;58(2):230-7.
Báez F, de Brun V, Rodriguez-Osorio N, Vinoles C. Low oxygen tension during
Bai L, Zhao Y, Zhou Y, Song Y, Xiao H, Zhao G, Wang Z, Li X. Advances in immunological sorting of X and Y chromosome-bearing sperm: from proteome to sex-specific proteins. Front Vet Sci. 2025;12:1523491.
Baldassarre H, Karatzas CN. Advanced assisted reproduction technologies (ART) in goats. Anim Reprod Sci. 2004;82-83:255-66.
Baldassarre H, Rao KM, Neveu N, Brochu E, Begin I, Karatzas CN. Laparoscopic ovum pick-up followed by
Baldassarre H. Laparoscopic ovum pick-up followed by
Banliat C, Mahe C, Lavigne R, Com E, Pineau C, Labas V, Guyonnet B, Mermillod P, Saint-Dizier M. The proteomic analysis of bovine embryos developed
Barbas JP, Leahy T, Horta AE, Garcia-Herreros M. Sperm kinematics and subpopulational responses during the cryopreservation process in caprine ejaculates. Cryobiology. 2018;82:137-47.
Bartlewski PM, Baby TE, Giffin JL. Reproductive cycles in sheep. Theriogenology. 2016;86:229-40.
Bastos NM, Ferst JG, Goulart RS, Silveira JC. The role of the oviduct and extracellular vesicles during early embryo development in bovine. Anim Reprod. 2022;19(1):e20220015.
Bathgate R. Functional integrity of sex-sorted, frozen-thawed boar sperm and its potential for artificial insemination. Theriogenology. 2008;70(8):1234-41.
Beilby KH, de Graaf SP, Evans G, Maxwell WMC, Wilkening S, Wrenzycki C, Grupen CG. Quantitative mRNA expression in ovine blastocysts produced from X- and Y-chromosome bearing sperm, both
Bó GA, Baruselli PS, Mapletoft RJ. Strategies to increment
Bragança GM, Batista RITP, Souza-Fabjan JMG, Alfradique VAP, Arashiro EKN, Pinto PHN, Santos JDR, Camargo LSA, Menchaca A, da Fonseca JF, Brandao FZ. Exogenous progestogens differentially alter gene expression of immature cumulus-oocyte complexes in sheep. Domest Anim Endocrinol. 2021a;74:106518.
Bragança GM, Alcantara-Neto AS, Batista RITP, Brandao FZ, Freitas VJF, Mermillod P, Souza-Fabjan JMG. Oviduct fluid during IVF moderately modulates polyspermy in
Catalá MG, Izquierdo D, Uzbekova S, Morato R, Roura M, Romaguera R, Papillier P, Paramio MT. Brilliant cresyl blue stain selects largest oocytes with highest mitochondrial activity, maturation-promoting factor activity and embryo developmental competence in prepubertal sheep. Reproduction. 2011;142(4):517-27.
Chen HH, Lee CI, Huang CC, Cheng EH, Lee TH, Lin PY, Chen CH, Lee MS. Biphasic oxygen tension promotes the formation of transferable blastocysts in patients without euploid embryos in previous monophasic oxygen cycles. Sci Rep. 2023;13(1):4330.
Chen Z, Robbins KM, Wells KD, Rivera RM. Large offspring syndrome: a bovine model for the human loss-of-imprinting overgrowth syndrome Beckwith-Wiedemann. Epigenetics. 2013;8(6):591-601.
Cognié Y, Baril G, Poulin N, Mermillod P. Current status of embryo technologies in sheep and goat. Theriogenology. 2003;59(1):171-88.
Cognié Y, Poulin N, Locatelli Y, Mermillod P. State-of-the-art production, conservation and transfer of
Coy P, Garcia-Vazquez FA, Visconti PE, Aviles M. Roles of the oviduct in mammalian fertilization. Reproduction. 2012;144(6):649-60.
Crozet N, Ahmed-Ali M, Dubos MP. Developmental competence of goat oocytes from follicles of different size categories following maturation, fertilization and culture
Cui J, Zhao X, Hao J, Liu X, Wang W, He L, Wang Y, Rong J, Qiu C, Chen D, Cheng L, Tian J, Zhang J, Xi G. Cross-species insights into
Cushman RA, Allan MF, Kuehn LA, Snelling WM, Cupp AS, Freetly HC. Evaluation of antral follicle count and ovarian morphology in crossbred beef cows: investigation of influence of stage of the estrous cycle, age, and birth weight. J Anim Sci. 2009;87(6):1971-80.
de Graaf SP, Evans G, Gillan L, Guerra MM, Maxwell WMC, O’Brien JK. The influence of antioxidant, cholesterol and seminal plasma on the
de Graaf SP, Evans G, Maxwell WMC, Cran DG, O’Brien JK. Birth of offspring of pre-determined sex after artificial insemination of frozen-thawed, sex-sorted and refrozen-thawed ram spermatozoa. Theriogenology. 2007b;67(2):391-8.
de la Fuente D, Maroto M, Cajas YN, Canon-Beltran K, Fernandez-Gonzalez R, Munoz-Maceda A, Sanchez-Puig JM, Blasco R, Cots P, Aviles M, Rizos D, Gutierrez-Adan A. Oviductin sets the species-specificity of the mammalian zona pellucida. eLife. 2025;14:e101338.
Diskin MG, Mackey DR, Roche JF, Sreenan JM. Effects of nutrition and metabolic status on circulating hormones and ovarian follicle development in cattle. Anim Reprod Sci. 2003;78(3-4):345-70.
Dos Santos-Neto PC, Cuadro F, Barrera N, Crispo M, Menchaca A. Embryo survival and birth rate after minimum volume vitrification or slow freezing of
Ealy AD, Wooldridge LK, McCoski SR. Board invited review: post-transfer consequences of
Ebrahimi M, Mara L, Chessa F, Angioi AG, Berlinguer F, Dattena M. Protocol for
Evans AC, Duffy P, Hynes N, Boland MP. Waves of follicle development during the estrous cycle in sheep. Theriogenology. 2000;53(3):699-715.
Falchi L, Ledda S, Zedda MT. Embryo biotechnologies in sheep: achievements and new improvements. Reprod Domest Anim. 2022;57(5, Suppl 5):22-33.
Ferraz MAMM, Ferronato GA. Opportunities involving microfluidics and 3D culture systems to the
Ferrer-Roda M, Gil A, Paramio MT, Izquierdo D. Effect of biphasic
Ferrer-Roda M, Izquierdo D, Gil A, Oliveira MEF, Paramio MT. Oocyte competence of prepubertal sheep and goat oocytes: an assessment of large-scale chromatin configuration and epidermal growth factor receptor expression in oocytes and cumulus cells. Int J Mol Sci. 2024b;25(8):4474.
Figueira LM, Alves NG, Souza-Fabjan JMG, Vergani GB, Oliveira MEF, Lima RR, Fonseca JF. Ultrasonographic cervical evaluation: a tool to select ewes for non-surgical embryo recovery. Reprod Domest Anim. 2020;55(11):1638-45.
Fonseca JF, Oliveira MEF, Brandao FZ, Batista RITP, Garcia AR, Bartlewski PM, Souza-Fabjan JMG. Non-surgical embryo transfer in goats and sheep: the Brazilian experience. Reprod Fertil Dev. 2019;31(1):17-26.
Fonseca JF, Vergani GB, Lima MSD, Silva KM, Monteiro AWU, Ramos AF, Alves BRC, Souza-Fabjan JMG, Oliveira MEF, Batista RITP. Nonsurgical embryo recovery as a feasible tool for supporting embryo biobanks of locally adapted Brazilian sheep and goats. Biopreserv Biobank. 2022;20(6):493-501.
Forcada F, Ait Amer-Meziane M, Abecia JA, Maurel MC, Cebrian-Perez JA, Muino-Blanco T, Asenjo B, Vazquez MI, Casao A. Repeated superovulation using a simplified FSH/eCG treatment for
Gandolfi F, Brevini TA, Cillo F, Antonini S. Cellular and molecular mechanisms regulating oocyte quality and the relevance for farm animal reproductive efficiency. Rev Sci Tech. 2005;24(1):413-23.
Gardner DK, Lane M. Culture and selection of viable blastocysts: a feasible proposition for human IVF? Hum Reprod Update. 1997;3(4):367-82.
Gibbons A, Pereyra Bonnet F, Cueto MI, Catala M, Salamone DF, Gonzalez-Bulnes A. Procedure for maximizing oocyte harvest for
Ginther OJ, Kot K, Kulick LJ, Wiltbank MC. Emergence and deviation of follicles during the development of follicular waves in cattle. Theriogenology. 1997;48(1):75-87.
Gonzalez-Bulnes A, Baird DT, Campbell BK, Cocero MJ, Garcia-Garcia RM, Inskeep EK, Lopez-Sebastian A, McNeilly AS, Santiago-Moreno J, Souza CJH, Veiga-Lopez A. Multiple factors affecting the efficiency of multiple ovulation and embryo transfer in sheep and goats. Reprod Fertil Dev. 2004;16(4):421-35.
Hansen PJ. The incompletely fulfilled promise of embryo transfer in cattle: why aren’t pregnancy rates greater and what can we do about it? J Anim Sci. 2020;98(11):skaa288.
Henkel RR, Schill WB. Sperm preparation for ART. Reprod Biol Endocrinol. 2003;1(1):108.
Hollinshead FK, Evans G, Evans KM, Catt SL, Maxwell WMC, O’Brien JK. Birth of lambs of a pre-determined sex after
Ireland JJ, Ward F, Jimenez-Krassel F, Ireland JL, Smith GW, Lonergan P, Evans AC. Follicle numbers are highly repeatable within individual animals but are inversely correlated with FSH concentrations and the proportion of good-quality embryos after ovarian stimulation in cattle. Hum Reprod. 2007;22(6):1687-95.
Jurado-Campos A, Soria-Meneses PJ, Arenas-Moreira M, Alonso-Moreno C, Rodriguez-Robledo V, Soler AJ, Garde JJ, Del Rocio Fernandez-Santos M. Minimizing sperm oxidative stress using nanotechnology for breeding programs in rams. J Anim Sci Biotechnol. 2023;14(1):106.
Kasinathan P, Wei H, Xiang T, Molina JA, Metzger J, Broek D, Kasinathan S, Faber DC, Allan MF. Acceleration of genetic gain in cattle by reduction of generation interval. Sci Rep. 2015;5(1):8674.
Leal CLV, Canon-Beltran K, Cajas YN, Hamdi M, Yaryes A, Millan de la Blanca MG, Beltran-Brena P, Mazzarella R, da Silveira JC, Gutierrez-Adan A, Gonzalez EM, Rizos D. Extracellular vesicles from oviductal and uterine fluids supplementation in sequential
Leoni GG, Rosati I, Succu S, Bogliolo L, Bebbere D, Berlinguer F, Ledda S, Naitana S. A low oxygen atmosphere during IVF accelerates the kinetic of formation of
Leroy JL, Vanholder T, Delanghe JR, Opsomer G, Van Soom A, Bols PE, Dewulf J, de Kruif A. Metabolic changes in follicular fluid of the dominant follicle in high-yielding dairy cows early
Lonergan P, Fair T. Maturation of oocytes
Lonergan P, Fair T. The ART of studying early embryo development: progress and challenges in ruminant embryo culture. Theriogenology. 2014;81(1):49-55.
Luvoni GC, Keskintepe L, Brackett BG. Improvement in bovine embryo production
Maia ALRS, Figueira LM, Rocha MS, Pinheiro JBS, Oliveira TA, Brandão FZ, Fonseca JF, Oliveira MEF, Oliveira CA, Souza-Fabjan JMG. The effect of flunixin meglumine on the premature regression of corpus luteum, recovery rate, and embryo production in superovulated Dorper ewes. Anim Reprod Sci. 2024;270:107595.
Mauchart P, Vass RA, Nagy B, Sulyok E, Bódis J, Kovács K. Oxidative stress in assisted reproductive techniques, with a focus on an underestimated risk factor. Curr Issues Mol Biol. 2023;45(2):1272-86.
Mazzarella R, Cajas YN, Gonzalez-Martínez ME, Rizos D. Extracellular vesicles: emerging paradigms in bovine embryo-maternal communication. Anim Reprod. 2024;21(3):e20240065.
Medina-Chávez DA, Sánchez-Ajofrín I, Peris-Frau P, Maside C, Montoro V, Fernández-Santos R, Garde JJ, Soler AJ. cAMP modulators before
Menchaca A, Barrera N, dos Santos Neto PC, Cuadro F, Crispo M. Advances and limitations of
Menchaca A, Hunton JR. SOP for small ruminant embryo technology. Part 1: In vivo embryo production. In: Leelawardana S, Perry G, Fiéni F, editors. Manual of the International Embryo Technology Society. 5th ed. Champaign: International Embryo Technology Society; 2025. Chap. 24, p. 290-296.
Menchaca A, Rubianes E, et al. Superovulation in sheep: follicular wave synchronization and embryo production. Theriogenology. 2009;72:477-83.
Mermillod P, Dalbiès-Tran R, Uzbekova S, Thélie A, Traverso JM, Perreau C, Papillier P, Monget P. Factors affecting oocyte quality: who is driving the follicle? Reprod Domest Anim. 2008;43(2, Suppl 2):393-400.
Mermillod P, Oussaid B, Cognié Y. Aspects of follicular and oocyte maturation that affect the developmental potential of embryos. J Reprod Fertil Suppl. 1999;54:449-60. PMid:10692875.
Meuwissen T, Hayes B, Goddard M. Genomic selection: a paradigm shift in animal breeding. Anim Front. 2016;6(1):6-14.
Morais MCC, Alcântara-Neto AS, Xavier-Getirana BR, Schmidt APP, Brandão FZ, Da Silveira JC, Renovato-Martins M, Nogueira FCS, Bustamante-Filho IC, Souza-Fabjan JMG. Extracellular vesicles in ewe blood plasma as biomarkers of hormonal treatment response and superovulation outcomes. Biol Reprod. 2025;113(6):1616-30.
Mortimer D. Sperm preparation methods. J Androl. 2000;21(3):357-66.
Natarajan R, Shankar MB, Munuswamy D. Effect of α-tocopherol supplementation on
Ngcobo JN, Nedambale TL, Sithole SM, Mtileni B, Mpofu TJ, Ramukhithi FV, Chokoe TC, Nephawe KA. A systematic review on the prospects of X- and Y-sexed semen in ruminant livestock: implications for conservation, a South African perspective. Front Vet Sci. 2024;11:1384768.
Palomo MJ, Mogas T, Izquierdo D, Paramio MT. The influence of sperm concentration, length of the gamete co-culture and the evolution of different sperm parameters on the
Paramio MT, Izquierdo D. Current status of
Paramio MT, Izquierdo D. Recent advances in
Peris SI, Morrier A, Dufour M, Bailey JL. Cryopreservation of ram semen facilitates sperm DNA damage: relationship between sperm andrological parameters and the sperm chromatin structure assay. J Androl. 2004;25(2):224-33.
Pinheiro JBS, Figueira LM, Correia LFL, Oliveira TA, Brair VL, Barros FFPC, Ascoli FO, Batista RITP, Brandão FZ, Souza-Fabjan JMG. The coasting time affects the quality of cumulus-oocyte complexes in superstimulated ewes. Theriogenology. 2023;196:236-43.
Reader KL, Stanton JL, Juengel JL. The role of oocyte organelles in determining developmental competence. Biology (Basel). 2017;6(3):35.
Rizos D, Clemente M, Bermejo-Alvarez P, De La Fuente J, Lonergan P, Gutiérrez-Adán A. Consequences of
Rizos D, Ward F, Duffy P, Boland MP, Lonergan P. Consequences of bovine oocyte maturation, fertilization or early embryo development
Rocha MS, Maia ALRS, Rangel PSC, Oliveira MEF, Fonseca JF, Oliveira CA, Souza-Fabjan JMG. Occurrence of premature regression of corpus luteum in MOET programs in Dorper ewes under subtropical climate. Livest Sci. 2022;255:104808.
Rodríguez-Dorta N, Cognié Y, González F, Poulin N, Guignot F, Touzé JL, Baril G, Cabrera F, Alamo D, Batista M, Gracia A, Mermillod P. Effect of coculture with oviduct epithelial cells on viability after transfer of vitrified
Rodríguez-González E, López-Béjar M, Velilla E, Paramio MT. Selection of prepubertal goat oocytes using the brilliant cresyl blue test. Theriogenology. 2002;57(5):1397-409.
Rodríguez-González E, López-Bejar M, Izquierdo D, Paramio MT. Developmental competence of prepubertal goat oocytes selected with brilliant cresyl blue and matured with cysteamine supplementation. Reprod Nutr Dev. 2003;43(2):179-87.
Romar R, Funahashi H, Coy P.
Rose RD, Gilchrist RB, Kelly JM, Thompson JG, Sutton-McDowall ML. Regulation of sheep oocyte maturation using cAMP modulators. Theriogenology. 2013;79(1):142-8.
Saint-Dizier M, Schoen J, Chen S, Banliat C, Mermillod P. Composing the early embryonic microenvironment: physiology and regulation of oviductal secretions. Int J Mol Sci. 2020;21(1):223.
Santos JDR, Ungerfeld R, Balaro MFA, Souza-Fabjan JMG, Cosentino IO, Brair VL, Souza CV, Pinto PHN, Bade ALC, Fonseca JF, Brandão FZ. Transcervical vs. laparotomy embryo collection in ewes: the effectiveness and welfare implications of each technique. Theriogenology. 2020;153:112-21.
Sirard MA. Folliculogenesis and acquisition of oocyte competence in cows. Anim Reprod. 2019;16(3):449-54.
Sirard MA, Richard F, Blondin P, Robert C. Contribution of the oocyte to embryo quality. Theriogenology. 2006;65(1):126-36.
Souza-Fabjan JMG, Locatelli Y, Freitas VJF, Mermillod P. Laparoscopic ovum pick up (LOPU) in goats: from hormonal treatment to oocyte possible destinations. Rev Bras Cienc Vet. 2014a;21(1):3-11.
Souza-Fabjan JM, Locatelli Y, Duffard N, Corbin E, Touzé JL, Perreau C, Beckers JF, Freitas VJ, Mermillod P.
Souza-Fabjan JMG, Rosa RM, Balaro MFA, Pinto PHN, Santos GB, Arashiro EKN, Fonseca JF, Ungerfeld R, Brandão FZ. Effect of different hormonal combinations on follicular wave emergence and superovulatory response in sheep. Theriogenology. 2017;103:24-9.
Souza-Fabjan JMG, Correia LFL, Batista RITP, Locatelli Y, Freitas VJF, Mermillod P. Reproductive seasonality affects
Souza-Fabjan JMG, Batista RITP, Melo LM, Oliveira MAL, Chaves MS, Fonseca JF, Freitas VJF. Transcervical versus laparotomy embryo recovery: what strategy is best for embryo bank formation in the Canindé goat conservation program? Biopreserv Biobank. 2022;20(2):204-7.
Souza-Fabjan JMG, Oliveira MEF, Guimarães MPP, Brandão FZ, Bartlewski PM, Fonseca JF. Non-surgical artificial insemination and embryo recovery as safe tools for genetic preservation in small ruminants. Animal. 2023a;17(Suppl 1):100787.
Souza-Fabjan JMG, Leal GR, Monteiro CAS, Batista RITP, Barbosa NO, Freitas VJF.
Teixeira PP, Padilha LC, Oliveira ME, Motheo TF, Silva AS, Barros FF, Coutinho LN, Flôres FN, Lopes MC, Bandarra MB, Silva MA, Vasconcelos RO, Rodrigues LF, Vicente WR. Laparoscopic ovum collection in sheep: gross and microscopic evaluation of the ovary and influence on oocyte production. Anim Reprod Sci. 2011;127(3-4):169-75.
Tervit HR, Whittingham DG, Rowson LE. Successful culture
Thompson JG.
Thompson JG, Lane M, Gilchrist RB. Metabolism of the bovine cumulus-oocyte complex and influence on subsequent developmental competence. Soc Reprod Fertil Suppl. 2007;64(1):179-90.
Tibary A, Anouassi A, Khatir H. Update on reproductive biotechnologies in small ruminants and camelids. Theriogenology. 2005;64(3):618-38.
Vanroose G, Nauwynck H, Van Soom A, Ysebaert MT, Charlier G, Van Oostveldt P, de Kruif A. Structural aspects of the zona pellucida of
Velilla E, Izquierdo D, Rodríguez-González E, López-Béjar M, Vidal F, Paramio MT. Distribution of prepubertal and adult goat oocyte cortical granules during meiotic maturation and fertilization: ultrastructural and cytochemical study. Mol Reprod Dev. 2004;68(4):507-14.
Velilla E, Rodríguez-González E, Vidal F, Paramio MT. Microtubule and microfilament organization in immature,
Velilla E, Rodríguez-González E, Vidal F, Izquierdo D, Paramio MT. Mitochondrial organization in prepubertal goat oocytes during
Viana JHM. 2023 statistics of embryo production and transfer in domestic farm animals. Embryo Technol Newsl. 2024;42(4):1-15.
Viana JHM. 2024 statistics of embryo production and transfer in domestic farm animals. Embryo Technol Newsl. 2025;43(4):1-15.
Wang L, Lin J, Huang J, Wang J, Zhao Y, Chen T. Selection of ovine oocytes by brilliant cresyl blue staining. J Biomed Biotechnol. 2012;2012:161372.
Wei X, Xiaoling Z, Kai M, Rui W, Jing X, Min G, Zhonghong W, Jianhui T, Xinyu Z, Lei A. Characterization and comparative analyses of transcriptomes for
Wieczorek J, Koseniuk J, Skrzyszowska M, Cegła M. L-OPU in goat and sheep: different variants of the oocyte recovery method. Animals (Basel). 2020;10(4):658.
Young LE, Sinclair KD, Wilmut I. Large offspring syndrome in cattle and sheep. Rev Reprod. 1998;3(3):155-63.
Zhu L, Marjani SL, Jiang Z. The epigenetics of gametes and early embryos and potential long-range consequences in livestock species-filling in the picture with epigenomic analyses. Front Genet. 2021;12:557934.
Submitted date:
03/15/2026
Accepted date:
04/23/2026
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