The effect of prolonged aflatoxin B1 (AFB1) administration on blood serum oestradiol-17β and progesterone concentrations in goats during the luteal phase and the synchronized oestrus was investigated. Thirty-six Greek indigenous primiparous goats were used, during the oestrus period; 12 goats received, per os, 50 μg (treated group T50) and 12 goats received 100 μg (treated group T100) AFB1/day/head, respectively, for approximately 1.5 month, while 12 goats served as controls (C). On day 36 of the experiment, each goat was injected, i.m, 0.5 ml prostaglandin F2α (PGF2α). Blood samples were collected from each goat twice a week, before PGF2α injection, as well as every 4 hours from the onset to the end of the synchronized oestrus. Oestradiol-17β and progesterone concentrations in blood serum were determined using radioimmunoassay. During the whole luteal(s) phase(s), linear regression analysis revealed a significant negative dependence (P < 0.05) of oestradiol-17β and a significant positive dependence (P < 0.05) of progesterone over group (C = 0, T50 = 50, T100 = 100), in a dose dependent manner. During the synchronized oestrus, multiple linear regression analysis revealed a significant negative dependence (P < 0.05) of oestradiol-17β, as well as a significant positive dependence (P < 0.05) of progesterone over group (C = 0, T50 = 50, T100 = 100) and over time (hours, from the onset to the end of the synchronized oestrus). No significant differences were noticed among the three groups, regarding the body weight of the goats from the onset to the end of AFB1 administration, the occurrence or the duration of the synchronized oestrus presented by the goats (P > 0.05). In conclusion, prolonged AFB1 administration at doses of 100 or even of 50 μg/day/head changes the hormonal pattern in blood during the luteal phase and the synchronized oestrus of goats, being in oestrus period.
CarterF , RingsF , MamoS , HolkerM , KuzmanyA , BesenfelderU , HavlicekV , MehtaJP , TesfayeD , SchellanderK , LonerganP . 2010. Effect of elevated circulating progesterone concentration on bovine blastocyst development and global transcriptome following endoscopic transfer of in vitro produced embryos to the bovine oviduct . Biol Reprod , 83 : 707 - 719 .
ChemineauP , GauthierD , PoirierJC , SaumandeJ . 1982 . Plasma levels of LH, FSH, Prolactin, Oestradiol-17β and Progesterone during natural and induced oestrus in the dairy goat . Theriogenology , 17 : 313 - 323 .
GalvanoF , GalofaroV , GalvanoG.1996 . Occurrence and stability of aflatoxin M1 in milk and milk products: a worldwide review . J Food Prot , 59 : 1079 - 1090 .
GiladE , MeidanR , BermanA , GraberY , WolfensonD.1993 . Effect of heat stress on tonic and GnRH-induced gonadotrophin secretion in relation to concentration of oestradiol in plasma of cyclic cows . J Reprod Fetril , 99 : 315 - 321 .
HasanzadehSh , HosseiniE , RezazadehL.2011 . Effects of aflatoxin B1 on profiles of gonadotropic (FSH and LH), steroid (testosterone and 17β-estradiol) and prolactin hormones in adult male rat . Iranian J Vet Res , 12 : 332 - 336 .
HuuskonenP , MyllynenP , StorvikM , PasanenM.2013 . The effects of aflatoxin B1 on transporters and steroid metabolizing enzymes in JEG-3 cells . Toxicol Lett , 218 : 200 - 206 .
IbehIN , SaxenaDK . 1997a . AFB1 and reproduction. I. Reproductive performance in female rats . Afr J Reprod Health , 1 : 79 - 84 .
IbehIN , SaxenaDK . 1997b . AFB1 and reproduction. II. Gametotoxicity in female rats . Afr J Reprod Health , 1 : 85 - 89 .
KlarićMS , CvetnićZ , PepeljnjakS , KosalecI . 2009 . Co-occurrence of aflatoxins, ochratoxin A, fumonisins, and zearalenone in cereals and feed, determined by competitive direct enzyme-linked immunosorbent assay and thin-layer chromatography . Arch Ind Hyg Toxicol , 60 : 427 - 434 .
KlichMA . 2007 . Aspergillus flavus the major producer of aflatoxin . Mol Plant Pathol , 8 : 713 - 722 .
KourousekosGD , TheodosiadouEK , LymberopoulosAG , BelibasakiS , BoscosC . 2015 . Effect of prolonged aflatoxin B1 administration on blood serum oestradiol-17β and progesterone concentrations of goats during the anoestrus period . Czech J Anim Sci , 60 : 164 - 170 .
KyreinHJ . 1974 . The binding affinity of aflatoxins on the uterine oestrogen receptor . Z Lebensm Unters Forsch , 154 : 285 - 287 .
MartinGB , SutherlandSRD , LindsayDR . 1987 . Effects of nutritional supplements on testicular size and the secretion of LH and testosterone in Merino and Booroola rams . Anim Reprod Sci , 12 : 267 - 281 .
MenchacaA , RubianesE.2001 . Effect of high progesterone concentrations during the early luteal phase on the length of the ovulatory cycle of goats . Anim Reprod Sci , 68 : 69 - 76 .
PartanenHA , El-NezamiHS , LeppanenJM , MyllynenPK , WoodhouseHJ , VahakangasKH . 2010 . Aflatoxin B1 transfer and metabolism in human placenta . Toxicol Sci , 113 : 216 - 225 .
TheodosiadouE , GoulasP , KouskouraT , SmokovitisA.2004 . Oestrogen and progesterone concentrations in plasma and oviductal tissue of ewes exhibiting a natural or induced oestrus . Anim Reprod Sci , 80 : 59 - 67 .
TheodosiadouE , AmiridisSG , TsiligianniT.2014 . Relationship between electrical resistance of cervical mucus and ovarian steroid concentration at the time of artificial insemination in ewes . Reprod Biol , 14 : 234 - 237 .
TheodosiadouE , TsiligianniT.2015 . Determination of the proper time for mating after oestrus synchronization during anoestrus or oestrus by measuring electrical resistance of cervical mucus in ewes . Vet Med-Czech , 60 : 87 - 93 .
WangikarPB , DwivediP , SinhaN , SharmaAK , TelangAG . 2005 . Effects of AFB1 on embryo fetal development in rabbits . Food Chem Toxicol , 43 : 607 - 615 .
Facebook
Google+
Twitter
LinkedIn
Mendeley
StumbleUpon
CiteULike
Reddit
Email