Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.21451/1984-3143-AR2017-0013
Animal Reproduction (AR)
Original Article

Influence of racial crossing on testicular structure and spermatogenesis in sheep Santa Inês and crossbred Santa Inês and Dorper

Morgana Santos Araújo, Isac Gabriel Cunha dos Santos, Jean Rodrigues Carvalho, Juanna D’Arc Fonseca Santos, Antônio Francisco da Silva Lisboa Neto, Amilton Cesar dos Santos, Manoel Lopes da Silva Filho, Feliciana Clara Fonseca Machado, Antonio Augusto Nascimento Machado Júnior

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Abstract

The study aimed at evaluating the testicular structure and spermatogenesis of Santa Inês and Santa Inês and Dorper crossbred sheep. Sixteen testicles of the animals under study were used, in order to evaluate the volumetric proportion, the diameter of the tubules, the height of seminiferous epithelium, the frequency of stages of the seminiferous epithelium cycle and the spermatogenic yield. The data were submitted to StudentNewman-Keulstestat 5% significance. The tubular diameter was 173.12 ± 29.09 μm and 185.71 ± 29.7 μm, and the height of the seminiferous epithelium was 52.29 ± 9.98 μm and 56.68 ± 11.25 μm, for the crossbred and the Santa Inês, respectively. There was no difference between the testicular compartments and the frequency of the stages of the seminiferous epithelium cycle between the groups. Santa Inês sheep presented a number of 15.36 ± 4.49 spermatocytes in the preleptotene/leptotene stage and 27.42 ± 6.65 spermatocytes in the pachytene, whereas the crossbred presented 13.18 ± 5.19 and 23.48 ± 7.80, respectively. The crossbred showed higher meiotic yield (3.98 ± 1.28) and spermatogenesis yield (3.71 ± 1.02). It is concluded there are differences in testicular morphology and spermatogenesis between Santa Inês sheep and crossbred Dorper/Santa Inês, indicating that the crossbreeding between the Santa Inês and Dorper animals allows a gain in the reproductive potential.

Keywords

sheep, spermatogenic efficiency, testicles.

References

Abercrombie M. 1946. Estimation of nuclear population from microtome sections. Anat Rec, 92:239- 247.

Almeida LM, Weiss RR, Castro CS, Büchele J. 2000. Quantificação histológica da espermatogênese em ratos Wistar tratados com dimetil sulfóxido. Arch Vet Sci, 5:129-135.

Amann RPA, Almquist J. 1962. Reproductive capacity of dairy bull. VIII. Direct and indirect measurement of testicular sperm production. J Dairy Sci, 45:774-781.

Amann RP. 1970. Sperm production rates. In: Jonhson AD, Gomes WR, Van Demark NL (Ed.). The Testis. New York, NY: Academic Press. pp. 433-482.

Andreussi PAT, Costa DS, Faria FJC, Fernandes CAC, Guimarães JD. 2014. Efficiency of the spermatogenesis in Zebu bulls (Bos taurus indicus). Anat Histol Embryol, 43:133-140.

Assis-Neto AC, Melo MIV, Carvalho MAM, Miglino MA, Oliveira MF, Ambrósio CE, Silva SMMS, Blasquez FXH, Papa PC, Kfoury Júnior JR. 2003.

Quantificação de células dos tubulos seminíferos e rendimento da espermatogênese em cutias (Dasyprocta aguti) criadas em cativeiro. Braz J Vet Res Anim Sci, 40:175-179.

Berndtson WE. 1977. Methods for quantifying mammalian spermatogenesis: a review. J Anim Sci, 5:818-883.

Carrijo Junior OA, Lucci CM, McManus C, Louvandini H, Martins RD, Amorim CA. 2008. Morphological evaluation of the testicles of young Santa Inês rams submitted to different regimes of protein supplementation and drenching. Ciênc Anim Bras, 9:433-441.

Castro ACS, Bernedson WE, Cardoso FM. 1997. Cinética e quantificação da espermatogênese: bases morfológicas e suas aplicações em estudos da reprodução de mamíferos. Rev Bras Reprod Anim, 21:25-34.

Costa DS, Henry M, Paula TAR. 2004. Espermatogênese de cateto (Tayassu tajacu). Arq Bras Med Vet Zootec, 56:46-51.

De Rooij DG, Lok D. 1987. Regulation of the density of spermatogonia in the seminiferous epithelium of the Chinese hamster: II. Differentiating spermatogonia. Anat Rec, 217:131-136.

Elias H, Hennig A, Schwartz DE. 1971. Stereology applications to biomedical research. Physiol Rev, 51:158-200.

França LR, Russell LD. 1998. The testis of domestic animals. In: Martinez-Garcia F, Regadera J (Ed.). Male Reproduction: a Multidisciplinary Overview. Madrid: Churchill Livingstone. pp. 197-219.

Furuta I, Porkka-Heiskanen T, Scarbrough K, Tapanainem J, Turek FW, Hsueh JW. 1994. Photoperiod regulates testis cell apoptosis in Djungarian hamsters. Biol Reprod., 51:1315-1321.

Instituto Nacional de Meteorologia – INMET/BEDMEP [homepage on the Internet]. 2016. Available on: http://www.inmet.gov.br/portal/index.php?r=bdmep/bd mep. Access on: April 19, 2016.

Machado Junior AAN, Oliveira LS, Assis Neto AC, Alves FR, Miglino MA, Carvalho MAM. 2012. Spermatogenesis in goats with and without scrotum bipartition. Anim Reprod Sci, 130:42-50.

Marai IFM, El-Darawany AA, Fadiel A, AbdelHafez MAM. 2008. Reproductive performance traits as affected by heat Stress and its alleviation in sheep: a review. Trop Subtrop Agroecosyst, 8:209-234.

Martins JAM, Souza CEA, Campos ACN, Aguiar GV, Lima ACB, Araújo AA, Neiva JNM, Moura AAA. 2008. Biometria do trato reprodutor e espermatogênese em ovinos sem padrão racial definido (SPRD). Arch Zootec, 57:553-556.

Maia MS, Medeiros IM, Lima CAC. 2011. Características Reprodutivas de carneiros no Nordeste do Brasil: parâmetros seminais. Rev Bras Reprod Anim, 35:175-179.

McManus C, Sasaki LCB, Louvandini H, Dias LT, Teixeira RA, Alves JM, Lucci CM, Marsiaj PHP, Murata LS. 2010. Avaliação histológica dos testículos de ovinos da raça santa inês nascidos em diferentes estações do ano. Rev Ciênc Rural, 40:396-402.

Nelson RJ, Kita M, Blom JM, Rhyne-Grey J. 1992. Photoperiod influences the critical caloric intake necessary to maintain reproduction among male deer mice (Peromyscus maniculatus). Biol Reprod, 46:226- 232.

Nunes AKR, Gouveia BB, Matos MHT, Pires IC., Franzo VS, Faria MD, Gradela A. 2013. Análise morfológica e funcional do processo espermatogênico em cobaios (Cavia porcellus) da pré-puberdade até a pós-puberdade. Pesq Vet Bras, 33:1-7.

Ortavant R, Courot M, Hochereau-De-Reviers MT. 1977. Spermatogenesis in domestic mammals. In: Cole HH, Cupps PT (Ed). Reproduction in Domestic Animals. 3rd ed. New York, NY: Academic Press. pp. 203-227.

Pacheco A, Quirino CR. 2010. Comportamento sexual em ovinos. Rev Bras Reprod Anim, 34:87-97.

Paula TAR, Costa DS, Matta SLP. 2002. Avaliação histológica quantitativa dos testículos de capivara (Hydrochoerus hydrochaeris) adultas. Biosci J, 18:121- 136.

Queiroz GC, Cardoso FM. 1989. Avaliação histológica do rendimento da espermatogênese de carneiros deslanados adultos. Rev Bras Reprod Anim, 13:99-108.

Rodrigues RTGA, Santos JRS, Azerêdo LMS, Rocha EF, Carvalho MAM, Portal MJID, Sousa OB, Menezes DJA. 2016. Influence of scrotal bipartition on spermatogenesis yield and Sertoli cell efficiency in sheep. Pesq Vet Bras, 36:258-262.

Santos JDF, Eufrasio RO, Pinheiro GFM, Alves FR, Carvalho MAM, Machado Júnior AAN. 2015. Influência da estação do ano do ano sobre a estrutura testicular em ovinos criados no sul do Estado do Piauí. Pesq Vet Bras 35:933-939.

Souza CEA. 2003. Avaliação da função reprodutiva de carneiros santa inês durante o primeiro ano de vida - desenvolvimento testicular, produção espermática e proteínas do plasma seminal. Fortaleza, CE, Universidade Federal do Ceará. Dissertation.

Sousa FML. 2010. Estudo das características do aparelho reprodutivo, epitélio seminífero e mapas eletroforéticos biodimensionais do plasma seminal de carneiros morada nova. Fortaleza, CE, Universidade Federal do Ceará. Dissertation.

Viu MAO, Oliveira Filho BD, Lopes DT, Viu AFM, Santos KJG. 2006. Fisiologia e manejo reprodutivos de ovinos: revisão. Rev Eletrôn Fac Montes Belos, Goiás, 1:79-98.

Wrobel KH, Reichold J, Schimmel M. 1995. Quantitative morphology of the ovine seminiferous epithelium. Ann Anat, 177:19-32.

Young KA, Nelson RJ. 2001. Mediation of seasonal testicular regression by apoptosis. Reproduction, 122:677-685.

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