Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.21451/1984-3143-AR2017-0040
Animal Reproduction (AR)
Original Articles

Fipronil-induced decrease in the epididymal sperm count: oxidative effect and protection by vitamin E

Meiriele Mazzo, Kamila Villas Boas Balieira, Paulo Francisco Veiga Bizerra, Fábio Erminio Mingatto

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Abstract

The toxic effects of the insecticide fipronil on the sperm production and oxidative damage in the testis were evaluated, as well as the protective action of vitamin E. Male rats received vehicle or fipronil 5 mg/kg and fipronil 5 mg/kg + vitamin E 100 mg/kg for 14 days. Thereafter, the sperm concentration in the epididymis and parameters of oxidative damage in the homogenate of testicles were assessed. Fipronil reduced epidydimal sperm count. The activity of the glutathione peroxidase enzyme increased and that of catalase was reduced in the testis. Also, a reduction in GSH and an increase in the concentration of malondialdehyde were observed in the animals treated with fipronil. The vitamin E reestablished the analysed parameters to levels similar to those of the control group. We concluded that fipronil decreased sperm production in rats because of its oxidant activity and that this effect was reversed by vitamin E.

Keywords

antioxidants, insecticide, oxidative stress, spermatozoids, testis.

References

Aajoud A, Ravanel P, Tissut M. 2003. Fipronil metabolism and dissipation in a simplified aquatic ecosystem. J Agric Food Chem, 51:1347-1352.

Aebi H, Bergmeyer HU. 1974. Catalase in methods of enzymatic analysis. 2.ed. New York, USA: Academic Press. pp.673-684.

Aitiken RJ, Baker MA. 2006. Oxidative stress sperm survival and fertility control. Mol Cel Endocrinol, 250:66-69.

Akiyama M. 1999. In vivo scavenging effect of ethylcysteine on reactive oxygen species in human semen. Jpn J Urol, 90:421-428.

Arkezenov MY, Markesbery WR. 2001. Changes in thiol content and expression of glutathione redox system genes in the hippocampus and cerebellum in Alzheimer’s disease. Neurosci Lett, 302:141-145.

Badgujar PC, Chandratre GA, Pawar NN, Telang AG, Kurade NP. 2015a. Fipronil induced oxidative stress involves alterations in SOD1 and catalase gene expression in male mice liver: Protection by vitamins E and C. Environ Toxicol, 31:1147-1158.

Badgujar PC, Pawar NN, Chandratre GA, Telang AG, Sharma AK. 2015b. Fipronil induced oxidative stress in kidney and brain of mice: Protective effect of vitamin E and vitamin C. Pestic Biochem Phys, 118:10-18.

Ball BA, Vo A. 2001. Osmotix tolerance of equine spermatozoa and the effects of soluble cryoprotectants on equine sperm motility viability and mitochondrial membrane potential. J Androl, 22:1061-1069.

Barros AL, Bae JH, Borges CS, Rose JL Cavariani MM, Silva PV, Pinheiro PFF, Anselmo-Franci JA, Arena AC. 2016. Perinatal exposure to insecticide fipronil: effects on the reproductive system in male rats. Reprod Fertil Dev. doi: 10.1071/RD15517.

Brzezinska-Slebodzinska E, Slebodzinski AB, Pietras B, Wieczorek G. 1995. Antioxidant effect of vitamin E and glutathione on lipid peroxidation in boar semen plasma. Biol Trace Elem Res, 47:69-74.

Buege JA, Aust SD. 1978. Microsomal lipid peroxidation. Methods Enzymol, 52:302-310.

Caboni P, Sammelson RE, Casida JE. 2003. Phenylpyrazole insecticide photochemistry metabolism and GABAergic action: ethiprole compared with fipronil. J Agr Food Chem, 51:7055-7061.

Cain K, Skilleter DN. 1987. Preparation and use of mitochondria in toxicological research. In Snell K, Mullock B (Eds.) Biochemical Toxicology. Oxford, UK: IRL Press. pp.217-254.

Calamera JC, Fernandez PJ, Buffone MG, Acosta AA, Doncel GF. 2001. Effect of long-term in vitro incubation of human spermatozoa: functional parameters and catalase effect. Androl, 33:79-86.

Chanton PF, Ravanel P, Tissut M, Meyran JC. 2001. Toxicity and bioaccumulation of fipronil in the nontarget arthropodan fauna associated with subalpine mosquito breeding. Ecotox Environ Safe, 52:8-12.

Cox C. 2005. Fipronil. J Pestic Sci, 25:10-15.

Droge W. 2002. Free radicals in the physiological control of cell function. Physiol Rev, 82:47-95.

El-Demerdash FM. 2004. Antioxidant effect of vitamin E and selenium on lipid peroxidation enzyme activities and biochemical parameters in rats exposed to aluminium. J Trace Elem Med Bio, 18:113-121.

El-Gendy KS, Aly NM, Mahmoud FH, Kenawy A, El-Seba AKH. 2010. The role of vitamin C as antioxidant in protection of oxidative stress induced by imidacloprid.  Food Chem Toxicol, 48:215-221.

Epa. 2017. Washington: Environmental Protection Agency Pesticides. Inc. Available in: http://www.epa.gov/pesticides. Accessed in: Jun 10th 2017.

Flohé L, Günzler WA. 1984. Assays of glutathione peroxidase. Methods Enzymol, 105:114-121.

Guelfi M, Maioli MA, Tavares MA, Mingatto FE. 2015. Citotoxicity of fipronil on hepatocytes isolated from rat and effects of its biotransformation. Braz Arch Biol Techn, 58:843-853.

Hainzl D, Cole LM, Casida JE. 1998. Mechanisms for selective toxicity of fipronil insecticide and its sulfone metabolite and desulfinyl photoproduct. Chem Res Toxicol, 11:1529-1535.

Halliwell B, Gutteridge JMC. 2007. Free radicals in biology and medicine. 4.ed. Oxford, USA: Oxford University Press. pp.704.

Hissin PJ, Hilf RA. 1976. A fluorometric method for determination of oxidized and reduced glutathione in tissues. Anal Biochem, 74:214-226.

Hollenbach, CB. 2013. Estudo da toxicidade reprodutiva do óleo essencial de orégano (Origanun vulgare L.) em ratos Wistar. Porto Alegre, RS, Brasil: Faculdade de Veterinária da Universidade Federal do Rio Grande do Sul. Thesis.

Hong Z, Hailing L, Hui M, Guijie Z, Leyan Y, Dubing Y. 2010. Effect of Vitamin E supplement in diet on antioxidant ability of testis in Boer goat. Anim Reprod Sci, 117:90-94.

Hsu PC, Liu MY, Hsu CC, Chen LY, Guo YL. 2001. Effects of vitamin E and/or C on reactive oxygen species-related lead toxicity in rat sperm. Toxicology, 128:169-179.

Leichert LI, Jakob U. 2004. Protein thiol modifications visualised in vivo. Plos Biol, 2:333.

Mossa ATH, Swelam ES, Mohafrash SMM. 2015. Sub-chronic exposure to fipronil induced oxidative stress biochemical and histopathological changes in the liver and kidney of male albino rats. Toxicol Rep, 2:775-784.

Noblanc A, Kocer A, Chabory E, Vernet P, Saez F, Cadet R, Conrad M, Drevet JR. 2011. Glutathione peroxidases at work on epididymal spermatozoa: an example of the dual effect of reactive oxygen species on mammalian male fertilizing ability. J Androl, 32:641-650.

Ohi M, Dalsenter PR, Andrade AJM, Nascimento AJ. 2004. Reproductive adverse effects of fipronil in Wistar rats. Toxicol Lett, 146:121-127.

Paim F. 2010. Controle seletivo do Rhipicephalus (Boophilus) microplus (Canestrini 1887) em bovinos criados em campo nativo com uso de fipronil e avaliação de sua eficácia em Lages–SC. Lages, Brasil: Centro de Ciências Agroveterinárias da Universidade do Estado de Santa Catarina. Dissertation.

Park K, Jeon S, Song YJ, Yi LS. 2012. Proteomic analysis of boar spermatozoa and quantity changes of superoxide dismutase 1, glutathione peroxidase, and peroxiredoxin 5 during epididymal maturation. Anim Reprod Sci, 135:53-61.

Pohanish RP. 2014. Sittig's Handbook of Pesticides and Agricultural Chemicals. 2.ed. Norwish, NY, USA: William Andrew Publishing. pp.412.

Reed DJ. 2001. Mechanisms of chemically induced cell injury and cellular protection mechanisms. In Hodgson E, Smart RC (Eds.) Introduction to Biochemical Toxicology. 3rd ed. New York, NY, USA: John Wiley & Sons. pp.221-253.

Ribeiro SMR, Queiroz JH, Peluzio MCG, Costa NMB, Matta SLP, Queiroz MELR. 2015. A formação e os efeitos das espécies reativas de oxigênio no meio biológico. Biosci J, 21:133-149.

Santana AT, Guelfi M, Medeiros HCD, Tavares MA, Bizerra PFV, Mingatto FE. 2015. Mechanisms involved in reproductive damage caused by gossypol in rats and protective effects of vitamin E. Biol Res, 48:43.

Sedlak J, Lindsay RH. 1968. Estimation of total protein-bound and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem, 25:192-205.

Sies H. 1986. Biochemistry of oxidative stress. Angew Chem-Ger Edit, 25:1058-1071.

Surai P, Kostjuk I, Wishart G, Macpherson A, Speake B, Noble R, Ionov I, Kutz E. 1998. Effect of Vitamin E and selenium supplementation of cockerel diets on glutathione peroxidase activity and lipid peroxidation susceptibility in sperm testes and liver. Biol Trace Elem Res, 64:119-132.

Tang J, Usmani KA, Hodgson E, Rose RL. 2004. In vitro metabolism of fipronil by human and rat cytochrome P450 and its interactions with testosterone and diazepam. Chem-Biol Interact, 147:319-329.

Tavares MA, Palma IDF, Medeiros HCD, Guelfi M, Santana AT, Mingatto FE. 2015. Comparative effects of fipronil and its metabolites sulfone and desulfinyl on the isolated rat liver mitochondria. Environ Toxicol Phar, 40:206-214.

Tingle CC, Rother JA, Dewhurst CF, Lauer S, King WJ. 2003. Fipronil: environmental fate, ecotoxicology, and human health concerns. Rev Environ Contam Toxicol, 176:1-66.

Watson PF. 2000. The causes of reduced fertility with cryopreserved semen. Anim Reprod Sci, 60-61:481-492.

Yousef MI, Abdallah GA, Kamel KI. 2003. Effect of ascorbic acid and Vitamin E supplementation on semen quality and biochemical parameters of male rabbits. Anim Reprod Sci, 76:99-111.

Yousef MI, Awad TI, Mohamed EH. 2006. Deltamethrin-induced oxidative damage and biochemical alterations in rat and its attenuation by Vitamin E. Toxicology, 227:240-247.

Yue D, Yan L, Luo H, Xu X, Jin X. 2010. Effect of Vitamin E supplementation on semen quality and the testicular cell membranal and mitochondrial antioxidant abilities in Aohan fine-wool sheep. Anim Reprod Sci, 118:217-222.

Zhao X, Yeh JZ, Salgado VL, Narahashi T. 2004. Fipronil is a potent open channel blocker of glutamate-activated chloride channels in cockroach neurons. J Pharmacol Exp Ther, 310:192-201.

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