Effects of hCG administration on accessory corpus luteum formation and progesterone production in estrous-induced nulliparous Santa Inês ewes
Jeferson Ferreira da Fonseca, Ana Carolina Rosa Castro, Eduardo Kenji Nunes Arashiro, Maria Emilia Franco Oliveira, Fabiana Nunes Zambrini, Luciana Vieira Esteves, Felipe Zandonadi Brandão, Joanna Maia Gonçalves Souza-Fabjan
Abstract
The effect of hCG administration on accessory corpus luteum (ACL) formation, CL area, and plasma progesterone (P4) concentration (ng/mL) seven days after breeding was studied in nulliparous Santa Inês sheep. Intravaginal 60 mg MAP sponges were inserted into ewes for six days and 300 IU eCG i.m. and 30 µg d-cloprostenol latero-vulvar were administered 24 h before sponge removal. Ewes were naturally bred and, seven days after first mating (Day 0; D0), were treated with either 250 IU hCG (hCG group; n = 7) or 1 mL saline solution (control group; n = 7). Blood was collected to determine plasma P4 concentrations and sonograms were performed on Days 7, 10, 13, 16, 19, and 22. Number of CL on D7 was similar (P > 0.05) between hCG (1.3 ± 0.5) and control (1.3 ± 0.5) groups; however, on D13, it was greater (P < 0.05) in the hCG group (2.3 ± 0.5) than in the control group (1.3 ± 0.5). A greater (P < 0.05) luteal tissue area was detected in hCG-treated ewes (n = 4) on Days 16 to 22 than in the animals in the control group (n = 7). Plasma P4 concentration on D13 to D22 was higher (P < 0.05) in hCG-treated animals than in control ewes. Administration of hCG seven days after estrus onset efficiently induced accessory CL formation in ewes, increasing luteal tissue area and plasma P4 concentration.
Keywords
References
Arashiro EKN, Viana JHM, Fonseca JF, Camargo LSA, Fernandes CAC, Brandão FZ. 2010. Luteal dynamics in goats: morphological and endocrine features. Rev Bras Zootec, 39:1937-1942.
Balaro MFA, Fonseca JF, Oba E, Cardoso, EC, Brandão FZ. 2014. Is the Santa Inês sheep a typical non-seasonal breeder in the Brazilian Southeast? Trop Anim Health Prod, 46:1533-1537.
Bartlewski PM, Beard AP, Rawlings NC. 1999. An ultrasonographic study of luteal function in breeds of sheep with different ovulation rates. Theriogenology, 52:115-130.
Cavalcanti AS, Brandão FZ, Garcia Nogueira LA, Fonseca JF. 2012. Effects of GnRH administration on ovulation and fertility in ewes subjected to estrous synchronization. Rev Bras Zootec, 41:1412-1418.
Diskin MG, Morris DG. 2008. Embryonic and Early Foetal Losses in Cattle and Other Ruminants. Reprod Domest Anim, 43(Suppl. 2):261-268.
Farin CE, Moeller CL, Mayan H, Gamboni F, Sawyer HR, Niswender GD. 1988. Effect of luteinizing hormone and human chorionic gonadotropin on cell populations in the ovine corpus luteum. Biol Reprod, 38:413-421.
Figueira LM, Fonseca JF, Arashiro EKN, SouzaFabjan JMG, Ribeiro ACS, Oba E, Viana JHM, Brandao FZ. 2015. Colour Doppler ultrasonography as a tool to assess luteal function in Santa Ines ewes. Reprod Domest Anim, 50:643-650.
Fonseca JF, Silva Filho JM, Pinto Neto A, Palhares MS, Ruas JRM, Alvim MTT, Belisário, H, Pardini WS. 2000. Indução de corpo lúteo acessório em novilhas. Rev Bras Reprod Anim, 24:143-147.
Fonseca JF, Silva Filho JM, Palhares MS, Ruas JRM, Pinto Neto A. 2001a. Concentração plasmática de progesterona em novilhas receptoras submetidas à administração de rbST, GnRH ou hCG no quinto dia do ciclo estral. Arq Bras Med Vet Zootec, 53:451-458.
Fonseca JF, Silva Filho JM, Pinto Neto A, Palhares MS, Ruas JRM. 2001b. Taxas de gestação de novilhas receptoras submetidas à administração de rbST, GnRH ou hCG no quinto dia do ciclo estral. Arq Bras Med Vet Zootec, 53:459-464.
Fonseca JF, Torres CAA. 2005. Administration of hCG 5 days after breeding and reproductive performance in nulliparous dairy goats. Reprod Dom Anim, 40:495-499.
Fonseca JF, Maffili VV, Rodrigues MT, Santos ADF, Rovay H, Pinto Neto A, Brandão FZ, Torres CAA. 2006. Effects of hCG on progesterone concentrations and fertility in cyclic, lactating Alpine goats. Anim Reprod, 3:410-414.
Fonseca JF, Souza-Fabjan JMG, Oliveira MEF, Cruz RC, Esteves LV, Matos de Paiva MPSL, Brandão FZ, Mancio AB. 2017. Evaluation of cervical mucus and reproductive efficiency of seasonally anovular dairy goats after short-term progestagen-based estrous induction protocols with different gonadotropins. Reprod Biol, 17(4):363-369.
Kittok RJ, Stellflug JN, Lowry SR. 1983. Enhanced progesterone and pregnancy rate after gonadotropin administration in lactating ewes. J Anim Sci, 56:652-655.
Mann GE, Robinson RS, Hunter MG. 2007. Corpus luteum size and function following single and double ovulations in non-lactating dairy cows. Theriogenology, 67:1256-1261.
Menchaca A, Rubianes E. 2004. New treatments associated with timed artificial insemination in small ruminants. Reprod Fertil Dev, 16:403-413.
Mexia AA, Macedo FAF, Alcalde Sakaguti ES, Martins EN, Yamamoto M, Macedo RMG. 2004. Desempenho reprodutivo e produtivo de ovelhas Santa Inês suplementadas diferentes fases da gestação. Rev Bras Zootec, 33:658-667.
National Research Council-NRC. 2007. Nutrient requirements of Small Ruminants. Washington, DC, Ed. Natl Acad Science, 384p.
Oliveira MEF, Ayres H, Oliveira LG, Barros, FFPC, Oba E, Bicudo SD, Bartlewski PM, Fonseca JF, Vicente WRR. 2016. Effects of season and ovarian status on the outcome of long-term progesterone-based estrus synchronization protocols and ovulatory follicle development in Santa Inês ewes under subtropical conditions. Theriogenology, 85:452-460.
Lonergan P, Forde N, Spencer TE. 2016. Role of progesterone in embryo development in cattle. Reprod Fertil Dev, 28:66-74.
Rhind SM, Chesworth JM, Robinson JJ. 1978. A seasonal difference in ovine peripheral plasma prolactin and progesterone concentrations in early pregnancy and in the relationship between the two hormones. J Reprod Fertil, 52:79-81.
Ribeiro Júnior, I. Análises estatÃsticas no SAEG, 2001. Editora UFV, Viçosa, 301.
Siqueira LGB, Torres CAA, Amorim LS, Souza ED, Camargo LSA, Fernandes CAC, Viana, JHM. 2009. Interrelationships among morphology, echotexture, and function of the bovine corpus luteum during the estrous cycle. Anim Reprod Sci, 115:18-28.
Tarso SGS, Apgar GA, Gastal, MO, Gastal EL. 2016. Relationships between follicle and corpus luteum diameter, blood flow, and progesterone production in beef cows and heifers: preliminary results. Anim Rep, 13:81-92.
Teixeira TA, Fonseca JF, Souza-Fabjan JMG, Carvalheira LR, Fernandes DAM, Brandão, FZ. 2016. Efficiency of different hormonal treatments for estrus synchronization in tropical Santa Inês sheep. Trop Anim Health Prod, 48:545-551.
Theodosiadou E, Goulas P, Kouskoura T, Smokovitis A. 2004. Oestrogen and progesterone concentrations in plasma and oviductal tissue of ewes exhibiting a natural or induced oestrus. Anim Reprod Sci, 80:59-67.
Wheeler AG, Barnes A. 1983. Variation in peripheral progesterone concentrations with environmental temperature in ewes on Days 7 and 11 of the oestrous cycle. J Reprod Fertil, 69:697-701.
Wiltbank MC. 1994. Cell types and hormonal jmechanisms associated with mid-cycle corpus luteum function. J Anim Sci, 72:1873-1883.
Facebook
Google+
Twitter
LinkedIn
Mendeley
StumbleUpon
CiteULike
Reddit
Email