Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.21451/1984-3143-AR2017-976
Animal Reproduction (AR)
Original Article

Simultaneous umbilical blood flow during normal pregnancy in sheep and goat foetuses using non-invasive colour Doppler ultrasound

Mohammed Ahmed Elmetwally. Sabine Meinecke-Tillmann

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Abstract

The characteristics of umbilical blood flow (UM) was investigated using 18 (25 foetuses) pregnant ewes and 20 (41 foetus) pregnant goats transrectal non-invasive color Doppler ultrasonographic examinations were done frequently between 2 and 8 week after breeding and then transabdominally until parturition. Colour Doppler velocimetery includes blood flow volume (BFV), time averaged maximum velocity (TAMV), resistance index (RI), pulsatility index (PI), time average of mean (TAMEAN) and impedance of blood flow (PS/ED or AB ratio). Also a qualitative evaluation of UM blood flow indicating increases (P < 0.001) in BFV, TAMV and TAMEAN were observed until 19 week of pregnancy in foetuses of sheep and goats and then those values decreased (P < 0.001) from 19 week until parturition. Conversely, UM-PI, RI and PS/ED decreased (P < 0.002- 0.01) until 19 week and then increased (P < 0.01-0.0001). The umbilical artery BFV increased (P < 0.0001) during pregnancy from 7.27 ± 0.82 ml/min in sheep vs. 4.96 ± 0.54 ml/min in goats at 6 week of gestation to 700.51 ± 31.05 ml/min (~100 fold) in sheep vs. 665.56 ± 48.22 ml/min (~133 fold) in goats at 19 week and then decreased (P < 0.0001) to 350.561 ± 72.15 ml/min in sheep vs. 215.17 ± 35.06 ml/min in goats at 20 week. The absence of end diastolic velocity (EDV) of umbilical artery blood flow was detected in both species between 4 and 12 week of pregnancy. Results of this study clearly show that the non-invasive colour Doppler sonography can be used successfully to assess umbilical blood flow in foetuses of pregnant sheep and goats. These may provide guidelines for assessing the state of intrauterine fetal growth retardation in pregnancies of sheep and goats.

Keywords

colour Doppler sonography, foetus, goats, umbilical, sheep

References

Acheson, GH, Dawes, GS, Mott JC. 1956. The relation of the O2 consumption of foetal and new-born lambs to the arterial O2 saturation. J Physiol, 133:11 pp.

Anderson SG, Hackshaw BT, Still JG, Greiss FC. 1977. Uterine blood flow and its distribution after chronic estrogen and progesterone administration. Am J Obstet Gynecol, 127:138-142.

Barcroft J, Flexner LB, McClurkin T. 1934. The output of the foetal heart in the goat. J Physiol, 82:498-508.

Barcroft J, Kennedy J A, Mason, MF. 1939. The blood volume and kindred properties in pregnant sheep. J Physiol, 95:159-172.

Bollwein H, Weber F, Woschee I, Stolla R. 2004. Transrectal Doppler sonography of uterine and umbilical blood flow during pregnancy in mares. Theriogenology, 61:499-509.

Cohnstein J, Zuntz N. 1884. Untersuchungen iiber das Blut, ben Kreislauf und die Athmung beim Saugethierfotus. Pfluegers Arch Ges Physiol, 34:173.

Cooper KE, Greenfield AD, Huggett AS. 1949. The umbilical blood flow in the foetal sheep. J Physiol, 108:160-166.

Coppens M, Loquet P, Kollen M, De Neubourg F, Buytaert P. 1996. Longitudinal evaluation of uteroplacental and umbilical blood flow changes in normal early pregnancy. Ultrasound Obstet Gynecol, 7:114-121.

Crenshaw C, Huckabee WE, Curet LB, Mann L, Barron, DH. 1968. A method for the estimation of the umbilical blood flow in unstressed sheep and goates with some results of its application. Q J Exp Physiol Cogn Med Sci, 53:65-75.

Dawes GS, Mott JC. 1964. Changes in O2 distribution and consumption in foetal lambs with variations in umbilical blood flow. J Physiol, 170:524-540.

Dawes GS. 1968. Fetal and Neonatal Physiology. Chicago: Year Book Medical Publishers, Inc. 248 pp.

Elmetwally M. 2012. Clinical applicability of noninvasive Doppler ultrasonography in small ruminants throughout pregnancy. Thesis Doctor of Veterinary Medicine, University of Veterinary Medicine Hannover, Hannover. Hannover, Germany. 151p.

Elmetwally M. 2016. Uterine blood flow indices in sheep during pregnancy. Quality in Primary Care, 24:197-202.

Elmetwally M, Bollwein H. 2016. Uterine blood flow in sheep and goats during the peri-parturient period assessed by transrectal Doppler sonography. Anim Reprod Sci, 176:32-39.

Elmetwally M, Rohn K,Meinecke-Tillmann S. 2016. Non-invasive color Doppler sonography of uterine blood flow throughout pregnancy in sheep and goats. Theriogenology, 85:1070-1079.

Erskine RL, Ritchie JW. 1985. Umbilical artery blood flow characteristics in normal and growth-retarded foetuses. Br J Obstet Gynaecol, 92:605-610.

FitzGerald DE, Drumm JE. 1977. Non-invasive measurement of human fetal circulation using ultrasound: a new method. Br Med J, 2:1450-1451.

Gill RW, Trudinger BJ, Garrett WJ, Kossoff G, Warren PS. 1981. Fetal umbilical venous flow measured in utero by pulsed Doppler and B-mode ultrasound. I. Normal pregnancies. Am J Obstet Gynecol, 139:720-725.

Hoskins PR, Haddad NG, Johnstone FD, Chambers SE, McDicken WN. 1989. The choice of index for umbilical artery Doppler waveforms. Ultrasound Med Biol, 15:107-111.

Kurjak A, Rajhvajn B. 1982. Ultrasonic measurements of umbilical blood flow in normal and complicated pregnancies. J Perinat Med, 10:3-16.

Link G, Clark KE, Lang U. 2007. Umbilical blood flow during pregnancy: evidence for decreasing placental perfusion. Am J Obstet Gynecol, 196:489, e1-7. doi: 10.1016/j.ajog.2006.11.017.

Makowski EL, Meschia G, Droegemueller W, Battaglia FC. 1968. Distribution of uterine blood flow in the pregnant sheep. Am J Obstet Gynecol, 101:409-412.

McCallum WD, Williams CS, Napel S, Daigle RE. 1978. Fetal blood velocity waveforms. Am J Obstet Gynecol, 132:425-429.

McGladdery SL, Aparicio S, Verrier-Jones K, Roberts R, Sacks SH. 1992. Outcome of pregnancy in an OxfordCardiff cohort of women with previous bacteriuria. Q J Med, 83:533-539.

Metcalfe J, Parer JT. 1966. Cardiovascular changes during pregnancy in ewes. Am J Physiol, 210:821-825.

Rudolph AM, Heymann MA. 1967. Validation of the antipyrine method for measuring fetal umbilical blood flow. Circ Res, 21:185-190.

Serin G, Gokdal O, Tarimcilar T, Atay O. 2010. Umbilical artery doppler sonography in Saanen goat foetuses during singleton and multiple pregnancies. Theriogenology, 74:1082-1087.

Soma LR, White RJ, Kane PB. 1971. Surgical preparation of a chronic maternal-fetal model in pregnant sheep: a technique for the measurement of middle uterine blood flow, umbilical blood flow, and fetal sampling in the awake sheep. J Surg Res, 11:85-94.

Stuart B, Drumm J, FitzGerald DE , Duignan NM. 1980. Fetal blood velocity waveforms in normal pregnancy. Br J Obstet Gynaecol, 87:780-785.

Trudinger BJ, Giles WB, Cook C M, Bombardieri J, Collins L. 1985. Fetal umbilical artery flow velocity waveforms and placental resistance: clinical significance. Br J Obstet Gynaecol, 92:23-30.

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