Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.21451/1984-3143-AR2018-0044
Animal Reproduction (AR)
Original Article

Use of two new formulations as bovine embryo manipulation solution

Flavio Guisseli Lopes, Eduardo Paulino da Costa, Vanessa Lopes Dias Queiroz-Castro, Emilio Cesar Martins Pereira, José Domingos Guimarães, Saullo Vinicius Pereira Alves, Carlos Antonio Carvalho Fernandes, Luiz Sérgio Almeida Camargo, Laercio dos Anjos Benjamim

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Abstract

This study aimed to evaluate the effect of two Embryo Manipulation Solutions (EMS and EMS supplemented) in maintenance of the viability of embryos, initially using structures derived from mice (first phase). Next, the efficiency of these solutions in routines of bovine embryo transfer was evaluated (second stage). Mice embryos were used in the stages of early blastocyst, and compact morula grades I and II. These embryos were initially randomly distributed and maintained for four hours in three solutions: Modified phosphate buffered saline (PBS; Control); EMS (treatment 1), and EMS supplemented (treatment 2). Subsequently, they were cultured in TCM 199 medium and evaluated in terms of total number of cells, morphometric characteristics, ultra structural aspects, detection of cell apoptosis, and quantification of Hsp70.3 gene expression. In the second phase, these same solutions were tested in the transfer of quality I and II bovine embryos (excellent and good). These embryos were transferred fresh to 58 recipients. The results showed that the total number of cells in embryos expanded blastocyst (ExB), the number of apoptotic cells, the cell, nuclear, nucleolar diameter and the nucleus/nucleolus ratio was similar among the treatments. The pregnancy rate shown on second phase was also similar. However, the EMS supplemented expressed more Hsp70.3 than EMS. The expression of Hsp70.3 was also greater for embryos in EMS than that of EMS supplemented. The McII embryos, EMS and EMS supplemented samples also expressed more Hsp70.3 compared to control embryos. In conclusion, the tested solutions can be used in routine embryo transfer techniques, replacing modified PBS solution as an effective media in maintaining embryo viability.

Keywords

cell apoptosis, Hsp 70.3, mice.

References

Absalón-Medina VA, Butler WR, Gilbert RO. 2014. Preimplantation embryo metabolism and culture systems: experience from domestic animals and clinical implications. J Assist Reprod Genet, 31(4):393-409.

Alexandre H. 1978. Correlation between cytological and morphogenetic effects of acute X-irradiation of preimplantation mouse embryos cultured in vitro. J Reprod Fertil, 53:399-402.

Bavister BD. 1995. Culture of preimplantation embryos: facts and artifacts. Hum Reprod Update, 1:91-148.

Beckmann RP, Lovett M, Welch MJ. 1992. Examining the function and regulation of Hsp70 in cells subjected to metabolic stress. J Cell Biol, 117:1137-1150.

Bowman P, McLaren A. 1970. Cleavage rate of mouse embryos in vivo and in vitro. J Embryol Exp Morphol, 24, 203-207.

Butcher l, Coates A, Martin KL, Rutherford AJ, Leese HJ. 1998. Metabolism of pyruvate by the early human embryo. Biol Reprod, 58:1054-1056.

Byrne AT, Southgate J, Brison DR, Leese HJ. 1999. Analysis of apoptosis in the preimplantation bovine embryo using TUNEL. J Reprod Fertil, 117:97-105.

Chun SO. 1973. Volume changes during the preimplantation stages of mouse egg development. Yonsei Med J, 14:63-90.

Costa EP, Lopes FG, Pereira ECM, Queiroz VLD, Macedo GG, Almeida Neto JRM, Costa AHA. 2010. Nova técnica para contagem do número de células de blastocistos [in Portuguese]. Arq Bras Med Vet Zootec, 62:1507-1510.

Costa EP, Vale Filho VR, Nogueira JC, Ferreira AM, Guimarães JD, Costa AHA. 1997. Cultivo in vitro de ovócitos bovinos em diferentes sistemas: I – efeito na maturação nuclear [in Portuguese]. Arq Bras Med Vet Zootec, 49:551-559.

Costa EP. 1994. Aspectos morfológicos (citológicos e ultraestruturais) e desenvolvimento de ovócitos de bovinos “in vitro” [in Portuguese]. Belo Horizonte, Brazil: Federal University of Minas Gerais. Thesis.

Dix DJ, Garges JB, Hong RL. 1998. Inhibition of hsp70-1 and hsp70-3 expression disrupts preimplantation embryogenesis and heightens embryo sensitivity to arsenic. Mol Reprod Dev, 51:373-380.

Donnay I, Leese HJ. 1999. Embryo metabolism during the expansion of the bovine blastocyst. Mol Reprod and Dev, 53:171-178.

Dorland M, Kruip TAM, Van Der Donk JA. 1992. Assessing day 7 bovine embryo viability by measuring the rate of nutrient uptake. J Reprod Fertil, 9:40.

El Hajj N, Haaf T. 2013. Epigenetic disturbances in in vitro cultured gametes and embryos: implications for human assisted reproduction. Fertil Steril, 99:632-641.

Evsikov V, Vagyna IN, Solomko AP. 1996. Mechanisms of cell number regulation in the peri-implantation mouse blastocyst. J Exp Zool, 276:201-208.

Fabian D, Bukovská AV, Koppel P, Makarevich A, Chrenek J. 2007. Chronological appearance of spontaneous and induced apoptosis during preimplantation development of rabbit and mouse embryos. Theriogenology, 68:1271-1281.

Fabian D, Koppel KJ, Hyttel-Maddox P. 2005. Apoptotic processes during mammalian preimplantation development. Theriogenology, 64:221-231.

Fear JM, Hansen PJ. 2011. Developmental changes in expression of genes involved in regulation of apoptosis in the bovine preimplantation embryo. Biol Reprod, 84:43-51.

Fukui Y, Ono H. 1989. Effects of sera, hormones and granulose cells added to culture medium for in vitro maturation, fertilization, cleavage and development of bovine oocytes. J Reprod Fertil, 86:501-506.

Gardner DK, Wale PL. 2013. Analises of metabolism to select viable human embryos for transfer. Fertil Steril, 99(4):1062-1072.

Gioso MM, Costa EP, Fernandes CAC, Torres CAA, Carvalho GR. 2005. Perfil de progesterona e intervalo ao estro de receptoras bovinas sincronizadas com doses reduzidas de cloprostenol [in Portuguese]. Rev Bras Zootec, 34:1181-1187.

Gordon I. 1994. Laboratory Production of Cattle Embryos CAB International, Cambridge UK: University Press.

Haas GTS, Fernandes CACF, Costa EP, Torres CAA, Marques PAF, Lopes FG, Paula TAR. 2007. Taxa de prenhez e concentração sérica de progesterona em novilhas receptoras de embrião tratadas com somatotropina recombinante bovina (rbST) [in Portuguese]. Ceres, 54(311):26-32.

Hoelker M, Schmoll F, Schneider H, Rings F, Gilles M, Tesfaye D, Jennen D, Tholen E, Griese J,Schellander K. 2006. Bovine blastocyst diameter as a morphological tool to predict embryo cell counts, embryo sex, hatching ability and developmental characteristics after transfer to recipients. Reprod Fertil Dev, 18:551-557.

Hoffmann RP. 1987. Diagnostico de parasitismo veterinário [in Portuguese]. Porto Alegre: Sulina, 156p.

Knijn HM, Gjorret JO, Vos Plam Hendriksen PJM, Van Der Weijden BC, Maddox-Hyttel P, Dieleman SJ. 2003. Consequences of in vivo development and subsequent culture on apoptosis, cell number, and blastocyst formation in bovine embryos. Biol Reprod, 69:1371-1378.

Lane M, Gardner DK. 1997. Nonessential amino acids and glutamine decrease the time of the first three cleavage divisions and increase compaction of mouse zygotes in vitro. J Assist Reprod Genet, 14:398-403.

Lazzari G, Wrenzycki C, Herrman D, Duchi R, Kruip T, Niemann H, Galli C. 2002. Cellular and molecular deviations in bovine in vitro-produced embryos are related to the large offspring syndrome. Biol Reprod, 67:767-775.

Levy RR, Cordonier H, Czyba JC, Guerin JF. 2001. Apoptosis in preimplantation mammalian embryo and genetics. Ital J Anat Embryol, 106:101-108.

Li CY, Lee JS, Ko YG, Kim JI, Seo JS. 2000. Heat Shock Protein 70 Inhibits Apoptosis Downstream of Cytochrome c Release and Upstream of Caspase-3 Activation. J Biol Chem, 275:25665-25671.

Lindner GM, Wright Junior RW. 1983. Bovine embryo morphology and evaluation. Theriogenology, 20:407-416.

Lonergan P, Rizos D, Kanka J, Nemcova L, Mbaye AM, Kingston M, Wade M, Duffy P, Boland MP. 2003. Temporal sensitivity of bovine embryos to culture environment after fertilization and the implications for blastocyst quality. Reproduction, 126:337-346.

Machado GM, Ferreira AR, Guardieiro MM, Bastos MR, Carvalho JO, Lucci CM, Diesel TO, Sartori R, Rumpf R, Franco MM. 2013. Morphology, sex ratio and gene expression of Day 14 in vivo and in vitro bovine embryos. Reprod Fertil Dev, 25:600-608.

Macmillan WH. 1998 Statistical models predicting embryo survival to term in cattle after embryo transfer. Theriogenology, 50:1053-1070.

Mantikou E, Youssef MAFM, Van Wely M, Van der Veen F, Al-Inany HG, Repping S, Mastenbroek S. 2013. Embryo culture media and IVF/ICSI success rates: a systematic review. Hum Reprod Update, 19:210-220.

Martin KL, Leese HJ. 1995. Role of glucose in mouse preimplantation embryo development. Mol Reprod Dev, 40:436-443.

Morales H, Tilquin P, Rees JF, Massip A, Dessy F, Van LA. 1999. Pyruvate prevents peroxide-induced injury of in vitro preimplantation bovine embryos. Mol Reprod Dev, 52:149-157.

Mori M, Otoi T, Suzuki T. 2002. Correlation between the cell number and diameter in bovine embryos produced in vitro. Reprod Domest Anim, 37:181-184.

Pedersen ME, Ozdas OB, Farstad W, Tverdal A, Olsaker I. 2005. Effects of bovine oviduct epithelial cells, fetal calf serum and bovine serum albumin on gene expression in single bovine embryos produced in the synthetic oviduct fluid culture system. Reprod Fertil Dev, 17:751-757.

Pereira DC, Dode MAN, Rumpf R. 2005. Evaluation of different culture systems on the in vitro production of bovine embryos. Theriogenology, 63:1131-1141.

Pfaffl MW, Georgieva TM, Georgiev IP,  Ontsouka E, Hageleit M, Blum JW. 2002. Real-time RT-PCR quantification of insulin-like growth factor (IGF)-1, IGF-1 receptor, IGF-2, IGF-2 receptor, insulin receptor, growth hormone receptor, IGF-binding proteins 1, 2 and 3 in the bovine species. Domest Anim Endocrinol, 22:91-102.

Ponsuksili S, Tesfaye D, El-Halawany N, Schellander K, Wimmers K. 2002. Stage-specific expressed sequence tags obtained during preimplantation bovine development by differential display RT-PCR and suppression subtractive hybridization. Prenat Diagn, 22:1135-1142.

Rafferty Junior KA. 1970. Methods in experimental embryology of the mouse. Baltimore: The John Hopkins Press, pp.23-29.

Rieger D. 1992. Relationships between energy metabolism and development of early mammalian embryos. Theriogenology, 37:75-93.

Riley JK, Moley KH. 2006. Glucose utilization and the PI3-K pathway: mechanisms for cell survival in preimplantation embryos. Reproduction, 131:823-835.

Rizos D, Gutierrez-Adan A, Perez-Garnelo S, de La Fuente J, Boland  MP, Lonergan P. 2003.  Bovine embryo culture in the presence or absence of serum: implications for blastocyst development, cryotolerance, and messenger RNA expression. Biol Reprod, 68:236-243.

Sagirkaya H, Misirlioglu M, Kaya A, First NL, Parrish JJ, Memili E. 2006. Developmental and molecular correlates of bovine preimplantation embryos. Reproduction, 131:895-904.

Sampaio IBM. 2002. Estatística aplicada experimentação animal [in Portuguese]. 2ªed. Belo Horizonte, Fundação de Estudo e Pesquisa em Medicina Veterinária e Zootecnia (FEPMVZ). 265p.

Smith R, McLaren A. 1977. Factors affecting the time of formation of the mouse blastocele. J Embryol Exp Morphol, 41:79-92.

Sociedade Internacional de Transferência de Embriões (IETS). 1998. Manual of the International Embryo Transfer Society.

Taylor WG. 1984. Toxicity and hazards to successful culture: cellular response to damage induced by light, oxygen or heavy metals. In: Patterson Jr MK. Uses and standardization of vertebrate cell cultures. In vitro monograph, n.5. Gaithersburg, MD:Tissue Culture Association, pp.58-70.

Thompson JG. 2000. In vitro culture and embryo metabolism of cattle and sheep embryos - a decade of achievement. Anim Reprod Sci, 60-61:263-275.

Universidade Federal de Viçosa (UFV). 1999. SAEG Sistema de análises estatísticas e genéticas. Versão 8.0.Viçosa [in Portuguese], MG: 1999. 59p. (Manual do usuário).

Vanda Ele L, Mateusen B, Maes DGD, Kruif A, Van Soom A. 2007. Temporal detection of caspase-3 and -7 in bovine in vitro produced embryos of different developmental capacity. Reprod Fertil, 133:709-718.

Vanroose G, Van Soom A, De Kruif A. 2001. From co-culture to defined medium: State of the art and practical considerations. Reprod Domest Anim, 36:25-28.

Viuff D, Hendriksen PJM, Vos PLAM, Dieleman SJ, Bibbiy BM, Greve T, Hyttel P, Thomsen PD. 2001. Chromosomal abnormalities and developmental kinetics in in vivo-developed cattle embryos at days 2 to 5 after ovulation. Biol Reprod, 65:204-208.

Whittingham DG. 1971. Culture of mouse ova. J Reprod Fertil Suppl, 48:7-21.

Zhang B, Penagaricano F, Driver A, Chen H, Khatib H. 2011. Differential expression of heat shock protein genes and their splice variants in bovine preimplantation embryos. J Dairy Sci, 94:4174-4182.

Zhu HB, Zhang ZH, Fadlalla E, Wang RX, Geng DF, Liu RZ. 2014. Culturing surplus poor-quality embryos to blastocyst stage have positive predictive value of clinical pregnancy rate. Iran J Reprod Med, 12(9):609-616.

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