Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.21451/1984-3143-AR2018-0059
Animal Reproduction (AR)
Conference Paper

Use of Doppler ultrasonography in embryo transfer programs: feasibility and field results

Guilherme Pugliesi, Gabriela Dalmaso de Melo, Gilmar Arantes Ataíde Jr, Carlos Augusto Gontijo Pellegrino, Júlio Barboza Silva, Cecília Constantino Rocha, Igor Garcia Motta, José Luiz Moraes Vasconcelos, Mario Binelli

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Abstract

The intensive use of Doppler ultrasonography in several studies in the last decade allowed the characterization of vascular perfusion and the estimation of function of the reproductive organs and tissues along the estrous cycle and pregnancy in cattle. We aim to discuss the possibility of using Doppler imaging and to explore the potential of its inclusion in reproductive programs in cattle industry. Recent studies in dairy and beef cows indicated a high accuracy and sensitivity when Doppler ultrasonography is used to evaluate corpus luteum function and to diagnosis pregnancy between days 20 and 22. Moreover, resynchronization programs starting 5 to 7 days after timed embryo transfer (FTET) coupled with early pregnancy diagnosis were developed for beef cattle, and have been implemented in commercial embryo transfer programs. These strategies allow a reduction in the interval between two FTET from ≈ 40 to 24 days and may improve the gains in reproductive efficiency when compared to traditional programs than begin resynchronization after the pregnancy diagnosis at 30 days. A second alternative to use Doppler imaging is the evaluation of luteal blood perfusion at the time of embryo transfer for selection of recipients with greater receptivity potential. This may increases fertility in FTET, as embryos would not be transferred to females with non-functional CL, and in cases with recipients surplus, females with higher receptivity would be prioritized.

Keywords

blood flow, corpus luteum, pregnancy. Uterus.

References

Acosta TJ, Hayashi KG, Matsui M, Miyamoto A. 2005. Changes in follicular vascularity during the first follicular wave in lactating cows. J Reprod Dev, 51:273- 280.

Assey RJ, Purwantara B, Greve T, Hyttel P, Schmidt MH. 1993. Corpus luteum size and plasma progesterone levels in cattle after cloprostenol-induced luteolysis. Theriogenology, 39:1321-1330.

Balaro MFA, Santos AS, Moura LFGM, Fonseca JF, Brandão FZ. 2017. Luteal dynamic and functionality assessment in dairy goats by luteal blood flow, luteal biometry, and hormonal assay. Theriogenology, 95:118- 126.

Bollwein H, Baumgartner U, Stolla R. 2002. Transrectal Doppler sonography of uterine blood flow in cows during pregnancy. Theriogenology, 57:2053- 2061.

Bollwein H, Lüttgenau J, Herzog K. 2012. Bovine luteal blood flow: basic mechanism and clinical relevance. Reprod Fertil Dev, 25:71-79.

Bollwein H, Heppelmann M, Lüttgenau J. 2016. Ultrasonographic Doppler Use for Female Reproduction Management. Vet Clin North Am Food Anim Pract, 32:149-164.

Cavallieri J. 2018. Effect of treatment of Bos indicus heifers with progesterone 0, 3 and 6 days after follicular aspiration on follicular dynamics and the timing of oestrus and ovulation. Anim Reprod Sci, 193:9-18.

DesCôteaux L, Carrière PD, Durocher J. 2005. Ultrasonography of the reproductive system of the cow: A 4 languages interactive CD-rom for continuing education of veterinarians. Continuing education services of the University of Montreal, St-Hyacinthe, Québec, Canada.

Diskin MG, Waters SM, Parr MH, Kenny DA. 2016. Pregnancy losses in cattle: potential for improvement. Reprod Fertil Dev, 28:83-93.

Ginther, OJ. Ultrasonic imaging and reproductive event in the mare. 1986. Equiservices Publishing: Cross Plains, WI, pp.1-12.

Ginther OJ. Ultrasonic imaging and animal reproduction: Horses. 1995. Equiservices Publishing: Cross Plains, WI, 394pp.

Ginther OJ. 1998. Ultrasonic Imaging and Animal Reproduction: Cattle. Equiservices Publishing: Cross Plains, WI, pp.29-58.

Ginther OJ. 2007. Ultrasonic Imaging and Animal Reproduction: Color-Doppler Ultrasonography. Book 4. Cross. Plains, WI: Equiservices Publishing, 258p.

Ginther OJ, Silva LA, Araujo RR, Beg MA. 2007. Temporal Associations among pulses of 13, 14- Dihydro-15-keto-PGF2alpha, Luteal Blood Flow, and Luteolysis in Cattle. Biol Reprod, 76:506-513.

Ginther OJ, Shrestha HK, Beg MA. 2010. Circulating hormone concentrations within a pulse of a metabolite of prostaglandin F2α during preluteolysis and early luteolysis in heifers. Anim Reprod Sci, 122:253-258.

Ginther OJ, Rakesh HB, Hoffman MM. 2014. Blood flow to follicles and CL during devel- opment of the periovulatory follicular wave in heifers. Theriogenology, 82:304-311.

Guimarães CRB, Oliveira ME, Rossi JR, Fernandes CAC, Viana JHM, Palhao MP. 2015. Corpus luteum blood flow evaluation on Day 21 to improve the management of embryo recipient herds. Theriogenology, v.84, p.237-241.

Hanzen C, Delsaux B. 1987. Use of transrectal B-mode ultrasound imaging in bovine pregnancy diagnosis. Vet Rec, 121:p.200-202.

Herzog K, Brockhan-Lüdemann M, Kaske M, Beindorff N, Paul V, Niemann H, Bollwein H. 2010.

Luteal blood flow is a more appropriate indicator for luteal function during the bovine estrous cycle than luteal size. Theriogenology, 73:691-697.

Kastelic JP, Curran S, Ginther OJ. 1989. Accuracy of ultrasonography for pregnancy diagnosis on days 10 to 22 in heifers. Theriogenology, 31, 813-820.

Kastelic JP, Bergfelt DR, Ginther OJ. 1990. Relationship between ultrasonic assessment of the corpus luteum and plasma progesterone concentration in heifers. Theriogenology, 33:1269-1278.

Kastelic JP, Bergfelt DR, Ginther OJ. 1991. Ultrasonic-detection of the conceptus and characterization of intrauterine fluid on days 10 to 22 in heifers. Theriogenology, 35, 569-581.

Lemes KM, Silva LA, Alonso MA, Celeghini ECC, Pugliesi G, Carvalho HF, Leite TG, Arruda RP. 2017. Uterine vascular perfusion and involution during the postpartum period in mares. J Equine Vet Sci, 51:61- 69.

Mann GE, Lamming GE. 2001. Relationship between maternal endocrine environment, early embryo development and inhibition of the luteolytic mechanism in cows. Reproduction, 121:175-180,

Matsui M, Miyamoto A. 2009. Evaluation of ovarian blood flow by colour Doppler ultrasound: practical use for reproductive management in the cow. Vet J, 181:232-240.

Mesquita FS, Pugliesi G, Scolari SC, França MR, Ramos RS, Oliveira M, Papa PC, Bressan FF, Meirelles FV, Silva LA, Nogueira GP, Membrive CM, Binelli M. 2014. Manipulation of the periovulatory sex steroidal milieu affects endometrial but not luteal gene expression in early diestrus Nelore cows. Theriogenology, 81:861-869.

Mitchell D. 1973. Detection of foetal circulation in the mare and cow by Doppler ultra-sound. Vet Rec, 93:365- 368.

Miyamoto A, Shirasuna K, Hayashi KG, Kamada D, Awashima C, Kaneko E, Acosta TJ, Matsui M. 2006. A potential use of color ultrasound as a tool for reproductive management: New observations using color ultrasound scanning that were not possible with imaging only in black and white. J Reprod Dev, 52:153- 160.

Nation DP, Malmo J, Davis GM, Macmillan KL. 2003.Accuracy of bovine pregnancy detection using transrectal ultrasonography at 28 to 35 days after insemination. Aust Vet J, 81:63-65.

Pieterse MC, Taverne MA, Kruip TA, Willemse AH. 1990. Detection of corpora lutea and follicles in cows: a comparison of transvaginal ultrasonography and rectal palpation. Vet Rec, 126:552-554.

Pierson RA, Ginther OJ. 1984. Ultrasonography for detection of pregnancy and study of embryonic development in heifers. Theriogenology, 22:225-233.

Pinaffi FLV, Santos ES, Silva MG, Maturana Filho M, Madureira EH, Silva LA. 2015. Follicle and corpus luteum size and vascularity as predictors of fertility at the time of artificial insemination and embryo transfer in beef cattle. Pesq Vet Bras, 35:470-476.

Pugliesi G, Khan FA, Hannan MA, Beg MA, Carvalho GR, Ginther OJ. 2012. Inhibition of prostaglandin biosynthesis during postluteolysis and effects on CL regression, prolactin, and ovulation in heifers. Theriogenology, 78:443-454.

Pugliesi G, Pinaffi FLV, Beg MA, Ginther OJ. 2013. Use of corpus luteum area as a predictor of ongoing functional luteolysis in dairy heifers. Reprod Fertil Dev, 25:235. (Abstract).

Pugliesi G, Miagawa BT, Paiva YN, França MR, Silva LA, Binelli M. 2014. Conceptus-induced changes in the gene expression of blood immune cells and the ultrasound-accessed luteal function in beef cattle: How early can we detect pregnancy? Biol Reprod, 95:1-12.

Pugliesi G, Santos FB, Lopes E, Nogueira É, Maio JR, Binelli M. 2016a. Improved fertility in suckled beef cows ovulating large follicles or supplemented with long-acting progesterone after timed-AI. Theriogenology, 85:1239-1248.

Pugliesi G, Silva JCB, Nishimura T, Miyai D, Silva LA, Binelli M. 2016b. Use of Color-Doppler ultrasonography to improve selection of higher fertility beef recipiente cows for embryo transfer. Anim Reprod, 13:454. (Abstract).

Pugliesi G, Rezende RG, Silva JCB, Lopes E, Nishimura TK, Baruselli PS, Madureira EH, Binelli M. 2017. Uso da ultrassonografia Doppler em programas de IATF e TETF em bovinos. Rev Bras Reprod Anim, 41:140-150.

Quintela LA, Barrio M, Peña AI, Becerra JJ, Cainzos J, Herradón PG, Díaz C. 2012. Use of ultrasound in the reproductive management of dairy cattle. Reprod Domest Anim, 47:34-44.

Rocha CC, Cardoso BO, Binelli M, Pugliesi G. 2017. Correlations between plasma progesterone and ultrasonographic characteristics of thecorpus luteum in fixed-time inseminated Nelore cows. Anim Reprod, 14:675. (Abstract).

Scully S, Evans AC, Duffy P, Crowe MA. 2014. Characterization of follicle and CL development in beef heifers using high resolution three-dimensional ultrasonography. Theriogenology, 81:407-418.

Scully S, Evans AC, Carter F, Duffy P, Lonergan P, Crowe MA. 2015. Ultrasound monitoring of blood flow and echotexture of the corpus luteum and uterus during early pregnancy of beef heifers. Theriogenology, 83:449-458.

Shrestha HK, Pugliesi G, Beg MA, Ginther OJ. 2011. Role of luteinizing hormone in changes in concentrations of progesterone and luteal blood flow during the hours of a simulated pulse of 13,14-dihydro15-keto-prostaglandin F(2alpha) (PGFM) in heifers. Biol Reprod, 85:482-489.

Siddiqui MAR, Almamun M, Ginther OJ. 2009. Blood flow in the wall of the preovulatory follicle and its relationship to pregnancy establishment in heifers. Anim Reprod Sci, 113:287-292.

Silva LA, Ginther OJ. 2010. Local effect of the conceptus on uterine vascular perfusion during early pregnancy in heifers. Reproduction, 139:453-463.

Siqueira LGB, Areas VS, Ghetti AM, Fonseca JF, Palhao MP, Fernandes CA, Viana JHM. 2013. Color Doppler flow imaging for the early detection of nonpregnant cattle at 20 days after timed artificial insemination. J Dairy Sci, 96:6461-6472.

Szatmári V, Sótonyi P, Vörös, K. 2001. Normal Duplex Doopler waveforms of major abdominal blood vessels in dogs: a Review. Vet Radiol Ultrasound, 42:93-107.

Utt MD, Johnson GL, Beal WE. 2009. The evaluation of corpus luteum blood flow using color-flow Doppler ultrasound for early pregnancy diagnosis in bovine embryo recipients. Theriogenology, 71:707-715.

Viana JHM, Arashiro EKN , Siqueira LGB , Ghetti AM, Areas VS , Guimarães CRB , Palhão MP, Camargo LSA, Fernandes CAC. 2013. Doppler ultrasonography as a tool for ovarian evaluation. Anim Reprod, 10:215-222.

Vieira LM, Sá Filho MF, Pugliesi G, Guerreiro BM, Cristaldo MA, Batista EOS, Freitas BG, Carvalho FJ, Guimarães LHC, Baruselli PS. 2014.

Resynchronization in dairy cows 13 days after TAI followed by pregnancy diagnosis based on corpus luteum vascularization by color Doppler. Anim Reprod, 11:378. (Abstract).

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