Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.21451/1984-3143-AR2018-0118
Animal Reproduction (AR)
Original Article

Effect of recombinant bovine somatotropin (rbST) treatment on follicular population and development in non-lactating dairy cows

Joao Alveiro Alvarado Rincón; Bruna Mion; Diego Andres Velasco Acosta; Bernardo Garziera Gasperin; Monique Tomazele Rovani; Lígia Margareth Cantarelli Pegoraro; Marcio Nunes Corrêa; Augusto Schneider

Downloads: 3
Views: 1698

Abstract

Abstract: The aim of this study was to evaluate the long-term effects of recombinant bovine somatotropin (rbST) on follicle population and ovulatory follicle development in non-lactating dairy cows. Twenty-one Jersey cows were allocated in rbST (n=11) or control (n=10) groups. On day -60, cows in rbST group received 500 mg of somatotropin (s.c. Lactotropin®, Elanco). On day 0, control and rbST cows received an intravaginal progesterone-releasing device (1.9 g, CIDR®, Zoetis) and GnRH (100 mg, IM, Factrel®, Zoetis). On day 8, cows received PGF2α (25 mg, IM, Lutalyse®, Zoetis) and the CIDR® was removed. Twelve hours after device removal (D8), serum, follicular fluid and granulosa cells samples were collected. Serum and follicular concentration of estradiol (E2) and progesterone (P4) were analyzed. Total RNA was extracted from granulosa cells to measure gene expression of LHCGR, STAR, HSD-3B1, CYP11A1, CYP19A1, CYP17A1, IGFR and PAPPA by real-time PCR. Ultrasonography was performed on days -60, -53, -46, -14, -7, 0 and 8 for antral follicle count. Results were analyzed by repeated measures ANOVA and t-test. There was no effect of rbST treatment on the number of follicles during the 60 days period, as well as no effect on serum and follicular fluid E2 and follicular fluid P4 at the moment of follicle aspiration. There was a reduction in PAPPA (P = 0.006), CYP11A1 (P = 0.04) and CYP19A1 (P = 0.002) mRNA levels in granulosa cells of the pre-ovulatory follicle of rbST treated cows. In conclusion, a single dose of rbST did not have long-term effects on antral follicle population, serum and follicular E2/P4 concentrations in non-lactating dairy cows. Despite that, rbST injection decreased granulosa cell expression of genes related to steroidogenesis in the pre-ovulatory follicle.

Keywords

follicular population, gene expression, steroidogenesis

References

Acosta DAV, Schneider A, Jacometo CB, Rincon JA, Cardoso F, Correa MN. Effect of bovine somatotropin injection in late pregnant Holstein heifers on metabolic parameters and steroidogenic potential of the first postpartum dominant follicle. Theriogenology. 2017;104:164-72. http://dx.doi.org/10.1016/j.theriogenology.2017.08.024. PMid:28863349.

Armstrong DG, Webb R. Ovarian follicular dominance: the role of intraovarian growth factors and novel proteins. Rev Reprod. 1997;2(3):139-46. http://dx.doi.org/10.1530/ror.0.0020139. PMid:9414477.

Bao B, Garverick HA. Expression of steroidogenic enzyme and gonadotropin receptor genes in bovine follicles during ovarian follicular waves: a review. J Anim Sci. 1998;76(7):1903-21. http://dx.doi.org/10.2527/1998.7671903x. PMid:9690647.

Bauman DE. Bovine somatotropin and lactation: from basic science to commercial application. Domest Anim Endocrinol. 1999;17(2-3):101-16. http://dx.doi.org/10.1016/S0739-7240(99)00028-4. PMid:10527114.

Bauman DE. Bovine somatotropin: review of an emerging animal technology. J Dairy Sci. 1992;75(12):3432-51. http://dx.doi.org/10.3168/jds.S0022-0302(92)78119-3. PMid:1474210.

Britt JH. Impacts of early postpartum metabolism on follicular development and fertility. Bovine Practitioner Proc. 1991;24:39-43.

Buratini J Jr, Price CA, Visintin JA, Bo GA. Effects of dominant follicle aspiration and treatment with recombinant bovine somatotropin (BST) on ovarian follicular development in nelore (Bos indicus) heifers. Theriogenology. 2000;54(3):421-31. http://dx.doi.org/10.1016/S0093-691X(00)00359-9. PMid:11051325.

Buratini J Jr, Teixeira AB, Costa IB, Glapinski VF, Pinto MG, Giometti IC, Barros CM, Cao M, Nicola ES, Price CA. Expression of fibroblast growth factor-8 and regulation of cognate receptors, fibroblast growth factor receptor-3c and -4, in bovine antral follicles. Reproduction. 2005;130(3):343-50. http://dx.doi.org/10.1530/rep.1.00642. PMid:16123241.

Butler ST, Marr AL, Pelton SH, Radcliff RP, Lucy MC, Butler WR. Insulin restores GH responsiveness during lactation-induced negative energy balance in dairy cattle: effects on expression of IGF-I and GH receptor 1A. J Endocrinol. 2003;176(2):205-17. http://dx.doi.org/10.1677/joe.0.1760205. PMid:12553869.

Butler ST, Pelton SH, Butler WR. Energy balance, metabolic status, and the first postpartum ovarian follicle wave in cows administered propylene glycol. J Dairy Sci. 2006;89(8):2938-51. http://dx.doi.org/10.3168/jds.S0022-0302(06)72566-8. PMid:16840609.

Butler WR, Smith RD. Interrelationships between energy balance and postpartum reproductive function in dairy cattle. J Dairy Sci. 1989;72(3):767-83. http://dx.doi.org/10.3168/jds.S0022-0302(89)79169-4. PMid:2654227.

Carlsson B, Bergh C, Bentham J, Olsson JH, Norman MR, Billig H, Roos P, Hillensjö T. Expression of functional growth hormone receptors in human granulosa cells. Hum Reprod. 1992;7(9):1205-9. http://dx.doi.org/10.1093/oxfordjournals.humrep.a137827. PMid:1478998.

Carriquiry M, Weber WJ, Dahlen CR, Lamb GC, Baumgard LH, Crooker BA. Production response of multiparous Holstein cows treated with bovine somatotropin and fed diets enriched with n-3 or n-6 fatty acids. J Dairy Sci. 2009;92(10):4852-64. http://dx.doi.org/10.3168/jds.2008-1673. PMid:19762801.

Cheong SH, Sa Filho OG, Absalon-Medina VA, Pelton SH, Butler WR, Gilbert RO. Metabolic and endocrine differences between dairy cows that do or do not ovulate first postpartum dominant follicles. Biol Reprod. 2016;94(1):18. http://dx.doi.org/10.1095/biolreprod.114.127076. PMid:26632612.

Cole WJ, Eppard PJ, Boysen BG, Madsen KS, Sorbet RH, Miller MA, Hintz RL, White TC, Ribelin WE, Hammond BG, Collier RJ, Lanza GM. Response of dairy cows to high doses of a sustained-release bovine somatotropin administered during two lactations. 2. Health and reproduction. J Dairy Sci. 1992;75(1):111-23. http://dx.doi.org/10.3168/jds.S0022-0302(92)77745-5. PMid:1541726.

De la Sota RL, Lucy MC, Staples CR, Thatcher WW. Effects of recombinant bovine somatotropin (sometribove) on ovarian function in lactating and nonlactating dairy cows. J Dairy Sci. 1993;76(4):1002-13. http://dx.doi.org/10.3168/jds.S0022-0302(93)77428-7. PMid:8486832.

Fenwick MA, Fitzpatrick R, Kenny DA, Diskin MG, Patton J, Murphy JJ, Wathes DC. Interrelationships between negative energy balance (NEB) and IGF regulation in liver of lactating dairy cows. Domest Anim Endocrinol. 2008;34(1):31-44. http://dx.doi.org/10.1016/j.domaniend.2006.10.002. PMid:17137745.

Ginther OJ, Beg MA. Dynamics of circulating progesterone concentrations before and during luteolysis: a comparison between cattle and Horses. Biol Reprod. 2012;86(6):170. http://dx.doi.org/10.1095/biolreprod.112.099820. PMid:22460665.

Gohary K, LeBlanc SJ, Lissemore KD, Overton MW, Von Massow M, Duffield TF. Effect of prepartum administration of recombinant bovine somatotropin on health and performance of lactating dairy cows. J Dairy Sci. 2014;97(10):6231-41. http://dx.doi.org/10.3168/jds.2014-8048. PMid:25087023.

Gong JG, Bramley T, Webb R. The effect of recombinant bovine somatotropin on ovarian function in heifers: follicular populations and peripheral hormones. Biol Reprod. 1991;45(6):941-9. http://dx.doi.org/10.1095/biolreprod45.6.941. PMid:1805998.

Grainger C, Wilhelms G, McGowan A. Effect of body condition at calving and level of feeding in early lactation on milk production of dairy cows. Aust J Exp Agric. 1982;22(115):9-17. http://dx.doi.org/10.1071/EA9820009.

Ireland JJ, Roche JF. Development of antral follicles in cattle after prostaglandin-induced luteolysis: changes in serum hormones, steroids in follicular fluid, and gonadotropin receptors. Endocrinology. 1982;111(6):2077-86. http://dx.doi.org/10.1210/endo-111-6-2077. PMid:6291909.

Ireland JL, Scheetz D, Jimenez-Krassel F, Themmen AP, Ward F, Lonergan P, Smith GW, Perez GI, Evans AC, Ireland JJ. Antral follicle count reliably predicts number of morphologically healthy oocytes and follicles in ovaries of young adult cattle. Biol Reprod. 2008;79(6):1219-25. http://dx.doi.org/10.1095/biolreprod.108.071670. PMid:18768912.

Jimenez-Krassel F, Binelli M, Tucker HA, Ireland JJ. Effect of long-term infusion with recombinant growth hormone-releasing factor and recombinant bovine somatotropin on development and function of dominant follicles and corpora lutea in Holstein cows. J Dairy Sci. 1999;82(9):1917-26. http://dx.doi.org/10.3168/jds.S0022-0302(99)75427-5. PMid:10509250.

Kassa T, Ambrose JD, Adams AL, Risco C, Staples CR, Thatcher MJ, Van Horn HH, Garcia A, Head HH, Thatcher WW. Effects of whole cottonseed diet and recombinant bovine somatotropin on ovarian follicles in lactating dairy cows. J Dairy Sci. 2002;85(11):2823-30. http://dx.doi.org/10.3168/jds.S0022-0302(02)74369-5. PMid:12487449.

Kawashima C, Kaneko E, Montoya CA, Matsui M, Yamagishi N, Matsunaga N, Ishii M, Kida K, Miyake Y, Miyamoto A. Relationship between the first ovulation within three weeks postpartum and subsequent ovarian cycles and fertility in high producing dairy cows. J Reprod Dev. 2006;52(4):479-86. http://dx.doi.org/10.1262/jrd.18003. PMid:16627955.

Kawashima C, Sakaguchi M, Suzuki T, Sasamoto Y, Takahashi Y, Matsui M, Miyamoto A. Metabolic profiles in ovulatory and anovulatory primiparous dairy cows during the first follicular wave postpartum. J Reprod Dev. 2007;53(1):113-20. http://dx.doi.org/10.1262/jrd.18105. PMid:17043386.

Kirby CJ, Smith MF, Keisler DH, Lucy MC. Follicular function in lactating dairy cows treated with sustained-release bovine somatotropin. J Dairy Sci. 1997a;80(2):273-85. http://dx.doi.org/10.3168/jds.S0022-0302(97)75935-6. PMid:9058268.

Kirby CJ, Wilson SJ, Lucy MC. Response of dairy cows treated with bovine somatotropin to a luteolytic dose of prostaglandin F2 alpha. J Dairy Sci. 1997b;80(2):286-94. http://dx.doi.org/10.3168/jds.S0022-0302(97)75936-8. PMid:9058269.

Lucy MC, Jiang H, Kobayashi Y. Changes in the somatotrophic axis associated with the initiation of lactation. J Dairy Sci. 2001;84:113-9. http://dx.doi.org/10.3168/jds.S0022-0302(01)70205-6.

Lucy MC. Fertility in high-producing dairy cows: reasons for decline and corrective strategies for sustainable improvement. Soc Reprod Fertil Suppl. 2007;64(1):237-54. http://dx.doi.org/10.5661/RDR-VI-237. PMid:17491151.

Lussier JG, Matton P, Dufour JJ. Growth rates of follicles in the ovary of the cow. J Reprod Fertil. 1987;81(2):301-7. http://dx.doi.org/10.1530/jrf.0.0810301. PMid:3430454.

Martinez MF, Sanderson N, Quirke LD, Lawrence SB, Juengel JL. Association between antral follicle count and reproductive measures in New Zealand lactating dairy cows maintained in a pasture-based production system. Theriogenology. 2016;85(3):466-75. http://dx.doi.org/10.1016/j.theriogenology.2015.09.026. PMid:26489910.

Mazerbourg S, Overgaard MT, Oxvig C, Christiansen M, Conover CA, Laurendeau I, Vidaud M, Tosser-Klopp G, Zapf J, Monget P. Pregnancy-associated plasma protein-A (PAPP-A) in ovine, bovine, porcine, and equine ovarian follicles: involvement in IGF binding protein-4 proteolytic degradation and mRNA expression during follicular development. Endocrinology. 2001;142(12):5243-53. http://dx.doi.org/10.1210/endo.142.12.8517. PMid:11713222.

Neunzig J, Milhim M, Schiffer L, Khatri Y, Zapp J, Sanchez-Guijo A, Hartmann MF, Wudy SA, Bernhardt R. The steroid metabolite 16(beta)-OH-androstenedione generated by CYP21A2 serves as a substrate for CYP19A1. J Steroid Biochem Mol Biol. 2017;167:182-91. http://dx.doi.org/10.1016/j.jsbmb.2017.01.002. PMid:28065637.

Nishimura R, Sakumoto R, Tatsukawa Y, Acosta TJ, Okuda K. Oxygen concentration is an important factor for modulating progesterone synthesis in bovine corpus luteum. Endocrinology. 2006;147(9):4273-80. http://dx.doi.org/10.1210/en.2005-1611. PMid:16740971.

Portela VM, Machado M, Buratini J Jr, Zamberlam G, Amorim RL, Goncalves P, Price CA. Expression and function of fibroblast growth factor 18 in the ovarian follicle in cattle. Biol Reprod. 2010;83(3):339-46. http://dx.doi.org/10.1095/biolreprod.110.084277. PMid:20484739.

Putnam DE, Varga GA, Dann HM. Metabolic and production responses to dietary protein and exogenous somatotropin in late gestation dairy cows. J Dairy Sci. 1999;82(5):982-95. http://dx.doi.org/10.3168/jds.S0022-0302(99)75318-X. PMid:10342237.

Rivera F, Narciso C, Oliveira R, Cerri RL, Correa-Calderon A, Chebel RC, Santos JE. Effect of bovine somatotropin (500 mg) administered at ten-day intervals on ovulatory responses, expression of estrus, and fertility in dairy cows. J Dairy Sci. 2010;93(4):1500-10. http://dx.doi.org/10.3168/jds.2009-2489. PMid:20338427.

Roche JF. The effect of nutritional management of the dairy cow on reproductive efficiency. Anim Reprod Sci. 2006;96(3-4):282-96. http://dx.doi.org/10.1016/j.anireprosci.2006.08.007. PMid:16996705.

Rodríguez FM, Colombero M, Amweg AN, Huber E, Gareis NC, Salvetti NR, Ortega HH, Rey F. Involvement of PAPP-A and IGFR1 in cystic ovarian disease in cattle. Reprod Domest Anim. 2015;50(4):659-68. http://dx.doi.org/10.1111/rda.12547. PMid:26031184.

Saccon TD, Moreira F, Cruz LA, Mondadori RG, Fang Y, Barros CC, Spinel L, Bartke A, Masternak MM, Schneider A. Ovarian aging and the activation of the primordial follicle reserve in the long-lived Ames dwarf and the short-lived bGH transgenic mice. Mol Cell Endocrinol. 2017;455:23-32. http://dx.doi.org/10.1016/j.mce.2016.10.015. PMid:27771355.

Santos JE, Juchem SO, Cerri RL, Galvao KN, Chebel RC, Thatcher WW, Dei CS, Bilby CR. Effect of bST and reproductive management on reproductive performance of Holstein dairy cows. J Dairy Sci. 2004;87(4):868-81. http://dx.doi.org/10.3168/jds.S0022-0302(04)73231-2. PMid:15259221.

Schneider A, Absalon-Medina VA, Esposito G, Correa MN, Butler WR. Paraoxonase (PON) 1, 2 and 3 expression in granulosa cells and PON1 activity in follicular fluid of dairy cows. Reprod Domest Anim. 2013;48(6):989-94. http://dx.doi.org/10.1111/rda.12198. PMid:23758560.

Schneider A, Schwegler E, Montagner P, Hax LT, Schmitt E, Pfeifer LF, Del Pino FA, Bianchi I, Paludo GR, Correa MN. Effect of prepartum somatotropin injection in late-pregnant Holstein heifers on metabolism, milk production and postpartum resumption of ovulation. Animal. 2012;6(6):935-40. http://dx.doi.org/10.1017/S1751731111002321. PMid:22558964.

Shimizu T, Murayama C, Sudo N, Kawashima C, Tetsuka M, Miyamoto A. Involvement of insulin and growth hormone (GH) during follicular development in the bovine ovary. Anim Reprod Sci. 2008;106(1-2):143-52. http://dx.doi.org/10.1016/j.anireprosci.2007.04.005. PMid:17507188.

Silva JR, Figueiredo JR, van den Hurk R. Involvement of growth hormone (GH) and insulin-like growth factor (IGF) system in ovarian folliculogenesis. Theriogenology. 2009;71(8):1193-208. http://dx.doi.org/10.1016/j.theriogenology.2008.12.015. PMid:19193432.

Silva PR, Soares HF, Braz WD, Bombardelli GD, Clapper JA, Keisler DH, Chebel RC. Effects of treatment of periparturient dairy cows with recombinant bovine somatotropin on health and productive and reproductive parameters. J Dairy Sci. 2017;100(4):3126-42. http://dx.doi.org/10.3168/jds.2016-11737. PMid:28215881.

Spicer LJ, Aad PY, Allen DT, Mazerbourg S, Payne AH, Hsueh AJ. Growth differentiation factor 9 (GDF9) stimulates proliferation and inhibits steroidogenesis by bovine theca cells: influence of follicle size on responses to GDF9. Biol Reprod. 2008;78(2):243-53. http://dx.doi.org/10.1095/biolreprod.107.063446. PMid:17959852.

Sudo N, Shimizu T, Kawashima C, Kaneko E, Tetsuka M, Miyamoto A. Insulin-like growth factor-I (IGF-I) system during follicle development in the bovine ovary: relationship among IGF-I, type 1 IGF receptor (IGFR-1) and pregnancy-associated plasma protein-A (PAPP-A). Mol Cell Endocrinol. 2007;264(1-2):197-203. http://dx.doi.org/10.1016/j.mce.2006.10.011. PMid:17116363.

Tajima K, Yoshii K, Fukuda S, Orisaka M, Miyamoto K, Amsterdam A, Kotsuji F. Luteinizing hormone-induced extracellular-signal regulated kinase activation differently modulates progesterone and androstenedione production in bovine theca cells. Endocrinology. 2005;146(7):2903-10. http://dx.doi.org/10.1210/en.2005-0093. PMid:15817663.

Taylor VJ, Cheng Z, Pushpakumara PGA, Wathes DC, Beever DE. Relationships between the plasma concentrations of insulin-like growth factor-I in dairy cows and their fertility and milk yield. Vet Rec. 2004;155(19):583-8. http://dx.doi.org/10.1136/vr.155.19.583. PMid:15573950.

Thatcher WW, Moreira F, Pancarci SM, Bartolome JA, Santos JE. Strategies to optimize reproductive efficiency by regulation of ovarian function. Domest Anim Endocrinol. 2002;23(1-2):243-54. http://dx.doi.org/10.1016/S0739-7240(02)00160-1. PMid:12142241.

Yakar S, Adamo ML. Insulin-like growth factor 1 physiology: lessons from mouse models. Endocrinol Metab Clin North Am. 2012;41(2):231-47. http://dx.doi.org/10.1016/j.ecl.2012.04.008. PMid:22682628.
 

5de4ff7e0e8825ec1d9f5039 animreprod Articles
Links & Downloads

Anim Reprod

Share this page
Page Sections