Immunohistochemical localization of progesterone receptors alpha (PRA) in ovary of the pseudopregnant rabbit
Mahmoud Abd-Elkareem and Alaa Sayed Abou-Elhamd
Abstract
Progesterone plays an important role in the reproductive function and follicular development in mammals. The aim of the present study was to examine the localization of progesterone receptor alpha (PRA) in ovary of pseudopregnant rabbit by immunohistochemical methods. Samples were collected from 14 h. to 18 days of pseudopregnancy. At the first stage of pseudopregnancy (14 h.), the rabbit ovary showed moderate immunostaining of PRA in the granulosa cells and theca interna cells of preovulatory follicle and in the stroma cells. At the middle stage of pseudopregnancy (3-7 days), the rabbit ovary showed strong immunostaining of PRA in ovarian surface epithelial cells, follicular cells of the primary follicle, granulosa cells and theca interna cells of the growing and antral follicles. Moderate immunoexpression of PRA were observed in the large lutein cells and endothelial cells of the corpus haemorrhagicum and corpus luteum and in the stroma cells. At the end of pseudopregnancy (18 days) strong PRA reactions were detected in the small lutein cells of the regressed corpus luteum. Moderate to strong PRA immuno-expression were observed in the proliferated theca interna cells of the atretic antral follicles. The atretic large lutein cells of the regressed corpus luteum showed negative immunostaining for PRA. This study showed that the PRA positive small lutein cells of the regressed corpus luteum and the PRA positive proliferated theca interna cells of the atretic antral follicles were transformed into PRA positive interstitial gland cells. In conclusion, the present study had described the distribution of PRA in the ovary of pseudopregnant rabbit, which is not discussed before in the available literature. It also gives more information about follicular dynamic, formation and origin of interstitial glands, mechanism of ovulation, formation and regression of the corpus luteum.
Keywords
References
Abd-Elkareem M. 2010. Histomorphological studies on the development of the ovary of the rabbits during the postnatal period. MSc, Assiut University, Assiut.
Abd-Elkareem M. 2014. Histomorphological studies on the ovary of the pregnant and pseudopregnant rabbits. PhD, Assiut University, Assiut.
Abd-Elkareem M. 2017a. Cell-specific immuno-localization of progesterone receptor alpha in the rabbit ovary during pregnancy and after parturition. Anim Reprod Sci, 180:100-120.
Abd-Elkareem M. 2017b. Morphological, Histological and Immunohistochemical Study of the Rabbit Uterus during Pseudopregnancy. J Cytol Histol, 8:1-7.
Anzaldua Arce, SR, Mendoza-Rodriguez, CA, Camacho-Arroyo, I, Cerbon, M, and Perez-Martinez, M. 2010. Progesterone receptor immunoreactivity differs in the uterus of pseudopregnant and medroxyprogesterone acetate-treated rabbits. Anim Reprod Sci, 120:173-178.
Blauer M, Heinonen PK, Martikainen PM, Tomas E, Ylikomi, T. 2005. A novel organotypic culture model for normal human endometrium: regulation of epithelial cell proliferation by estradiol and medroxyprogesterone acetate. Hum Reprod, 20:864-871.
Camacho-Arroyo, I, Gonzalez-Arenas, A, Gonzalez-Aguero, G, Guerra-Araiza, C, and Gonzalez-Moran, G. 2003. Changes in the content of progesterone receptor isoforms and estrogen receptor alpha in the chick brain during embryonic development. Comp Biochem Physiol A Mol Integr Physiol, 136:447-452.
Conneely OM, Mulac-Jericevic B, DeMayo F, Lydon JP, O'Malley BW. 2002. Reproductive functions of progesterone receptors. Recent Prog Horm Res, 57:339-355.
Conneely OM, Mulac-Jericevic B, Lydon JP. 2003. Progesterone-dependent regulation of female reproductive activity by two distinct progesterone receptor isoforms. Steroids, 68:771-778.
D'Haeseleer M, Simoens P, Van den Broeck, W. 2007. Cell-specific localization of progesterone receptors in the bovine ovary at different stages of the oestrous cycle. Anim Reprod Sci, 98:271-281.
Dharmarajan AM, Goodman SB, Atiya N,
Parkinson SP, Lareu RR, Tilly KI, Tilly JL. 2004. Role of apoptosis in functional luteolysis in the pregnant rabbit corpus luteum: evidence of a role for placental-derived factors in promoting luteal cell survival. Apoptosis, 9:807-814.
Dubreuil G. 1962. Corps progestatifs et gestatifs leurs varietes et leurs modes d'evolution chez les vertebres vivipares. Ann d'Endocr, 23:1-14.
Duffy DM, Wells TR, Haluska GJ, Stouffer RL. 1997. The ratio of progesterone receptor isoforms changes in the monkey corpus luteum during the luteal phase of the menstrual cycle. Biol Reprod, 57:693-699.
Durlej M, Tabarowski Z, Slomczynska M. 2010. Immunohistochemical study on differential distribution of progesterone receptor A and progesterone receptor B within the porcine ovary. Anim Reprod Sci, 121:67-73.
Gadsby JE, Keyes PL, Bill CH 2nd. 1983. Control of corpus luteum function in the pregnant rabbit: role of estrogen and lack of a direct luteotropic role of the placenta. Endocrinology, 113:2255-2262.
Gava N, Clarke CL, Byth K, Arnett-Mansfield RL, deFazio A. 2004. Expression of progesterone receptors A and B in the mouse ovary during the estrous cycle. Endocrinology, 145:3487-3494.
Greenwald GS, Rothchild I. 1968. Formation and maintenance of corpora lutea in laboratory animals. J Anim Sci, 27(Suppl 1):139-162.
Hegele-Hartung C, Chwalisz K, Beier HM. 1992. Distribution of estrogen and progesteron receptors in the uterus: an immunohistochemical study in the immature and adult pseudopregnant rabbit. Histochemistry, 97:39-50.
Hild-Petito S, Stouffer RL, Brenner RM. 1988. Immunocytochemical localization of estradiol and progesterone receptors in the monkey ovary throughout the menstrual cycle. Endocrinology, 123:2896-2905.
Hulas-Stasiak M, Gawron, A. 2010. Distribution of androgen and progesterone receptors in the spiny mouse (Acomys cahirinus) ovary during postnatal life. Reprod Biol, 10:37-51.
Iwai T, Nanbu Y, Iwai M, Taii S, Fujii S, Mori, T. 1990. Immunohistochemical localization of oestrogen receptors and progesterone receptors in the human ovary throughout the menstrual cycle. Virchows Arch A Pathol Anat Histopathol, 417:369-375.
Iwai T, Fujii S, Nanbu Y, Nonogaki H, Konishi I, Mori T, Okamura H. 1991. Effect of human chorionic gonadotropin on the expression of progesterone receptors and estrogen receptors in rabbit ovarian granulosa cells and the uterus. Endocrinology, 129:1840-1848.
Jacobs BR, Suchocki S, Smith RG. 1980. Evidence for a human ovarian progesterone receptor. Am J Obstet Gynecol, 138:332-336.
Keyes PL, Armstrong DT. 1968. Endocrine role of follicles in the regulation of corpus luteum function in the rabbit. Endocrinology, 83: 509-515.
Keyes PL, Gadsby JE. 1987. Role of estrogen and the placenta in the maintenance of the rabbit corpus luteum. Adv Exp Med Biol, 219:361-378.
Korte JM, Isola JJ. 1988. An immunocytochemical study of the progesterone receptor in rabbit ovary. Mol Cell Endocrinol, 58:93-101.
Kraus WL, Katzenellenbogen BS. 1993. Regulation of progesterone receptor gene expression and growth in the rat uterus: modulation of estrogen actions by progesterone and sex steroid hormone antagonists. Endocrinology, 132:2371-2379.
Kubota K, Cui W, Dhakal P, Wolfe MW, Rumi MA, Vivian JL, Roby KF, Soares MJ. 2016. Rethinking progesterone regulation of female reproductive cyclicity. Proc Natl Acad Sci U S A, 113:4212-4217.
Lan RX, Liu F, He ZB, Chen C, Liu SJ, Shi Y, Liu YL, Yoshimura Y, Zhang M. 2014. Immunolocalization of GnRHRI, gonadotropin receptors, PGR, and PGRMCI during follicular development in the rabbit ovary. Theriogenology, 81:1139-1147.
Maranesi M, Zerani M, Lilli L, Dall'Aglio C, Brecchia G, Gobbetti A, Boiti C. 2010. Expression of luteal estrogen receptor, interleukin-1, and apoptosis-associated genes after PGF2alpha administration in rabbits at different stages of pseudopregnancy. Domest Anim Endocrinol, 39:116-130.
Marcinkiewicz JL, Moy ES, Bahr JM. 1992. Change in responsiveness of rabbit corpus luteum to prostaglandin F-2 alpha during pregnancy and pseudopregnancy. J Reprod Fertil, 94:305-310.
Maybin JA, Duncan WC. 2004. The human corpus luteum: which cells have progesterone receptors? Reprod Camb Engl, 128:423-431.
Mills TM, Savard K. 1973. Steroidogenesis in ovarian follicles isolated from rabbits before and after mating. Endocrinology, 92:788-791.
Mulac-Jericevic B, Conneely OM. 2004. Reproductive tissue selective actions of progesterone receptors. Reproduction, 128:139-146.
Murdoch WJ. 1998. Perturbation of sheep ovarian surface epithelial cells by ovulation: evidence for roles of progesterone and poly(ADP-ribose) polymerase in the restoration of DNA integrity. J Endocrinol, 156:503-508.
Parillo F, Dall'Aglio C, Brecchia G, Maranesi M, Polisca A, Boiti C, Zerani M. 2013. Aglepristone (RU534) effects on luteal function of pseudopregnant rabbits: steroid receptors, enzymatic activities, and hormone productions in corpus luteum and uterus. Anim Reprod Sci, 138:118-132.
Park-Sarge OK, Parmer TG, Gu Y, Gibori G. 1995. Does the rat corpus luteum express the progesterone receptor gene? Endocrinology, 136:1537-1543.
Peluso JJ. 2006. Multiplicity of Progesterone’s Actions and Receptors in the Mammalian Ovary. Biol Reprod, 75:2-8.
Quirk SM, Cowan RG, Harman RM. 2004. Progesterone receptor and the cell cycle modulate apoptosis in granulosa cells. Endocrinology, 145:5033-5043.
Revelli A, Pacchioni D, Cassoni P, Bussolati G, Massobrio M. 1996. In situ hybridization study of messenger RNA for estrogen receptor and immunohistochemical detection of estrogen and progesterone receptors in the human ovary. Gynecol Endocrinol, 10:177-186.
Robker RL, Russell DL, Espey LL, Lydon JP, O'Malley BW, Richards JS. 2000. Progesterone-regulated genes in the ovulation process: ADAMTS-1 and cathepsin L proteases. Proc Natl Acad Sci U S A, 97:4689-4694.
Rueda BR, Hendry IR, Hendry IW, Stormshak F, Slayden OD, Davis JS. 2000. Decreased progesterone levels and progesterone receptor antagonists promote apoptotic cell death in bovine luteal cells. Biol Reprod, 62:269-276.
Sasano H, Suzuki T. 1997. Localization of steroidogenesis and steroid receptors in human corpus luteum. Classification of human corpus luteum (CL) into estrogen-producing degenerating CL, and nonsteroid-producing degenerating CL. Semin Reprod Endocrinol, 15:345-351.
Setty SL, Mills TM. 1987. The effects of progesterone on follicular growth in the rabbit ovary. Biol Reprod, 36:1247-1252.
Slomczynska M, Krok M, Pierscinski A. 2000. Localization of the progesterone receptor in the porcine ovary. Acta Histochem, 102:183-191.
Spencer TE, Bazer FW. 2002. Biology of progesterone action during pregnancy recognition and maintenance of pregnancy. Front Biosci, 7:d1879- d1898.
Teilmann SC, Clement CA, Thorup J, Byskov AG, Christensen ST. 2006. Expression and localization of the progesterone receptor in mouse and human reproductive organs. J Endocrinol, 191:525-535.
Van den Broeck W, D'Haeseleer M, Coryn M, Simoens P. 2002. Cell-specific distribution of progesterone receptors in the bovine ovary. Reprod Domest Anim, 37:164-170.
Vazquez, F, Rodriguez-Manzaneque, JC, Lydon, JP, Edwards, DP, O'Malley, BW, and Iruela-Arispe, ML. 1999. Progesterone regulates proliferation of endothelial cells. J Biol Chem, 274: 2185-2192.
Vermeirsch H, Simoens P, Coryn M, Van den Broeck W. 2001. Immunolocalization of progesterone receptors in the canine ovary and their relation to sex steroid hormone concentrations. Reproduction, 122:73-83.
Zerani M, Dall'Aglio C, Maranesi M, Gobbetti A, Brecchia G, Mercati F, Boiti, C. 2007. Intraluteal regulation of prostaglandin F2 alpha-induced prostaglandin biosynthesis in pseudopregnant rabbits. Reproduction, 133:1005-1016.
Facebook
Google+
Twitter
LinkedIn
Mendeley
StumbleUpon
CiteULike
Reddit
Email