Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.21451/1984-3143-AR2018-0147
Animal Reproduction (AR)
Original Article

Detection of Mycobacterium avium subsp. paratuberculosis in reproductive tissue and semen of naturally infected rams

José Vicente Velázquez-Morales; Marco Antonio Santillán-Flores; Jaime Gallegos-Sánchez; Juan Manuel Cuca-García; María del Carmen Navarro-Maldonado; Reyna Isabel Rojas-Martínez; César Cortez-Romero

http://dx.doi.org/10.21451/1984-3143-AR2018-0147 Anim Reprod, vol.16, n4, p.930-937, 2019
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Abstract

Abstract: Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of paratuberculosis (PTB), disease that causes a syndrome of bad nutrient absorption, weight loss and eventually death. The intestine is the main target organ where the infection develops; however, there is evidence of infection by MAP in extra-intestine sites of sheep, including mesenteric nodes and semen. The aim of the study was to identify the presence of MAP in reproductive tissue and semen of infected Pelibuey rams in clinical state of PTB. Seven rams were used in clinical PTB state and a non-infected ram by MAP of the Pelibuey breed, confirmed by serology, nPCR and bacteriological culture, with average weight and age of 57.23 ± 1.73 kg and 2.91 ± 0.17 years, respectively. The presence of MAP was identified in different tissue samples: spleen (1/7, 14.3% and 2/7, 28.6%), small intestine (3/7, 42.9% and 4/7, 57.1%) and mesenteric lymph nodes (3/7, 42.9% and 3/7, 42.9%), with nPCR and culture, respectively. It was also identified in epididymis tissue (1/7, 14.3%), Cowper gland (2/7, 28.6%) and prostate (1/7, 14.3%), using nPCR, although without detection in culture. It was identified in testicular tissue in 42.8% (3/7; culture or nPCR technique), but in 28.6% (2/7) with both techniques. Finally, the presence of MAP was identified in 42.9% (3/7) of semen samples with nPCR; however, it was not detected through culture. In conclusion, the presence of MAP was identified in lymphatic, digestive tissue, and semen; the presence of MAP was reported for the first time in epididymis, Cowper gland, prostate and testicles of infected Pelibuey rams.

Keywords

Mycobacterium avium, paratuberculosis, tissues, semen, Pelibuey ram

References

Ayele WY, Bartos M, Svastova P, Pavlik I. Distribution of Mycobacterium avium subsp. paratuberculosis in organs of naturally infected bull-calves and breeding bulls. Vet Microbiol. 2004;103(3-4):209-17. http://dx.doi.org/10.1016/j.vetmic.2004.07.011. PMid:15504592.

Ayele WY, Macháčková M, Pavlík I. The transmission and impact of paratuberculosis infection in domestic and wild ruminants. Vet Med Czech. 2001;46(7-8):205-24. http://dx.doi.org/10.17221/7878-VETMED.

Bergstein-Galan TG, Weiss RR, Kozicki LE, Bicudo SD. Sperm subpopulations in ejaculated sperm and spermatozoa recovered from ovine epididymides up to 48 h after death. Anim Reprod Sci. 2017;187:20-7. http://dx.doi.org/10.1016/j.anireprosci.2017.10.001. PMid:29029874.

Bielanski A. A review of the risk of contamination of semen and embryos during cryopreservation and measures to limit cross-contamination during banking to prevent disease transmission in ET practices. Theriogenology. 2012;77(3):467-82. http://dx.doi.org/10.1016/j.theriogenology.2011.07.043. PMid:21958629.

Buergelt CD, Donovan GA, Williams JE. Identification of Mycobacterium avium subspecies paratuberculosis by polymeerase chain reaction in blood and semen of a bull with clinical paratuberculosis. Int J Appl Res Vet Med. 2004;2:130-4.

Chávez-Gris G, Tavera-Trigo FJ, Svastova P, Pavlik I. Identificación del polimorfismo genético de aislamientos de Mycobacterium avium subespecie paratuberculosis de caprinos del centro de México. Veterinaria (Mex). 2004;35(1):75-82.

Chiodini RJ, Van Kruiningen HJ, Merkal RS. Ruminant paratuberculosis (Johne’s disease): the current status and future prospects. Cornell Vet. 1984;74(3):218-62. PMid:6375961.

Clarke CJ. The pathology and pathogenesis of paratuberculosis in ruminants and other species. J Comp Pathol. 1997;116(3):217-61. http://dx.doi.org/10.1016/S0021-9975(97)80001-1. PMid:9147244.

Colpos. Reglamento para el uso y cuidado de animales destinados a la investigación en el Colegio de Postgraduados [https://www.colpos.mx]. Montecillo: Colegio de Postgraduados; 2016. p. 1-16.

Eppleston J, Whittington R. Isolation of Mycobacterium avium subsp paratuberculosis from the semen of rams with clinical Johne’s disease. Aust Vet J. 2001;79(11):776-7. http://dx.doi.org/10.1111/j.1751-0813.2001.tb10898.x. PMid:11789915.

Erume J, Spergser J, Rosengarten R. Rapid detection of Mycobacterium avium subsp. paratuberculosis from cattle and zoo animals by Nested PCR. Afr Health Sci. 2001;1(2):83-9. PMid:12789121.

Fernández M, Benavides J, Sevilla IA, Fuertes M, Castaño P, Delgado L, García Marín JF, Garrido JM, Ferreras MC, Pérez V. Experimental infection of lambs with C and S-type strains of Mycobacterium avium subspecies paratuberculosis: immunological and pathological findings. Vet Res. 2014;45(1):5-15. http://dx.doi.org/10.1186/1297-9716-45-5. PMid:24428881.

García AB, Shalloo L. Invited review: the economic impact and control of paratuberculosis in cattle. J Dairy Sci. 2015;98(8):5019-39. http://dx.doi.org/10.3168/jds.2014-9241. PMid:26074241.

García E. Modificaciones al sistema climático de Köppen. 5th ed. México: Instituto de Geografía de la Universidad Nacional Autónoma de México; 2004.

Ghadiali AH, Strother M, Naser SA, Manning EJB, Sreevatsan S. Mycobacterium avium subsp. paratuberculosis strains isolated from crohn’s disease patients and animal species exhibit similar polymorphic locus patterns. J Clin Microbiol. 2004;42(11):5345-8. http://dx.doi.org/10.1128/JCM.42.11.5345-5348.2004. PMid:15528739.

Glawischnig W, Awad-Masalmeh M, Khaschabi D, Schonbauer M. Detection of Mycobacterium avium subsp. paratuberculosis from the testicles of a clinically infected breeding animal. Berl Munch Tierarztl Wochenschr. 2004;117(3-4):136-9. PMid:15046460.

Guzmán-Ruiz CC, Santillán-Flores MA, Córdova-López D. Prevalence and possible risk factors for caprine paratuberculosis in intensive dairy production units in Guanajuato, México. Veterinary Medicine and Animal Health. 2016;8(11):156-62. http://dx.doi.org/10.5897/JVMAH2016.0497.

Hasonova L, Trcka I, Babak V, Rozsypalova Z, Pribylova R, Pavlík I. Distribution of Mycobacterium avium subsp. paratuberculosis in tissues of naturally infected cattle as affected by age. Vet Med (Praha). 2009;54(6):257-69. http://dx.doi.org/10.17221/54/2009-VETMED.

Hemalatha S, Roy P, Purushothaman V, Iyue M. Paratuberculosis in different breeds of sheep : A retrospective study of cases. Int J Mycobacteriol. 2013;2(3):166-70. http://dx.doi.org/10.1016/j.ijmyco.2013.07.002. PMid:26785985.

Khol JL, Kralik P, Slana I, Beran V, Aurich C, Baumgartner W, Pavlík I. Consecutive excretion of Mycobacterium avium subspecies paratuberculosis in semen of a breeding bull compared to the distribution in feces, tissue and blood by IS900 and F57 quantitative real-time PCR and culture examinations. J Vet Med Sci. 2010;72(10):1283-8. http://dx.doi.org/10.1292/jvms.10-0052. PMid:20460836.

Larsen AB, Kopecky KE. Mycobacterium paratuberculosis in reproductive organs and semen of bulls. Am J Vet Res. 1970;31(2):255-8. PMid:5414867.

Larsen AB, Stalheim O, Hughes D, Appell L, Richards W, Himes E. Mycobacterium paratuberculosis in the semen and genital organs of a semen-donor bull. J Am Vet Med Assoc. 1981;179(2):169-71. PMid:7263470.

Martínez-Covarrubias AG, Santillán-Flores MA, Ruiz-Guzmán CC, Favila-Humara L C, Córdova-López D, Efrén-Díaz A, Hernández-Andrade L, Blanco-Ochoa MÁ. Desarrollo de un inmuno-ensayo enzimático (ELISA) para el diagnóstico de paratuberculosis en bovinos. Rev Mex Cienc Pecu. 2012;3:1-18.

Mcgregor H, Abbott KA, Whittington RJ. Effects of Mycobacterium avium subsp. paratuberculosis infection on serum biochemistry, body weight and wool growth in Merino sheep: A longitudinal study. Small Rumin Res. 2015;125:146-53. http://dx.doi.org/10.1016/j.smallrumres.2015.02.004.

Morón-Cedillo F, Cortez-Romero C, Gallegos-Sánchez J, Figueroa-Sandoval B, Aquino-Pérez G, Amante-Orozco A. Prevalencia de la infección por Mycobacterium avium subespecie paratuberculosis en rebaños de ovinos de dos municipios de San Luis Potosí, México. Revista Científica, FCV-LUZ. 2013;XXIII(4):293-9.

Nebbia P, Robino P, Zoppi S, De Meneghi D. Detection and excretion pattern of Mycobacterium avium subspecies paratuberculosis in milk of asymptomatic sheep and goats by Nested-PCR. Small Rumin Res. 2006;66(1-3):116-20. http://dx.doi.org/10.1016/j.smallrumres.2005.07.049.

Perry GH, Vivanco H, Holmes I, Gwozdz JM, Bourne J. No evidence of Mycobacterium avium subsp. paratuberculosis in in vitro produced cryopreserved embryos derived from subclinically infected cows. Theriogenology. 2006;66(5):1267-73. http://dx.doi.org/10.1016/j.theriogenology.2006.02.052. PMid:16672160.

Pieper L, Sorge U, Godkin A, DeVries T, Lissemore K, Kelton D. Management practices and their potential influence on johne’s disease transmission on canadian organic dairy farms: a conceptual analysis. Sustainability. 2014;6(11):8237-61. http://dx.doi.org/10.3390/su6118237.

Sagarpa. Norma Oficial Mexicana (NOM-033–ZOO–1995), sacrificio humanitario de los animales domésticos y silvestres [homepage on the Internet]. Ciudad de México: Secretaría de Agricultura, Ganadería, Desarrollo Rural, Pesca y Alimentación; 1996 [cited 2018 Dec 27]. Available from: http://sagarpa.gob.mx

Sagarpa. Norma Oficial Mexicana (NOM-062-ZOO-1999), especificaciones técnicas para la producción, cuidado y uso de los animales de laboratorio [homepage on the Internet]. Ciudad de México: Secretaría de Agricultura, Ganadería, Desarrollo Rural, Pesca y Alimentación; 1999 [cited 2018 Dec 27]. Available from: http://sagarpa.gob.mx

Sharifzadeh A, Doosti A, Fazeli MH, Adavoudi I. Nested PCR on semen samples for the detection of Mycobacterium avium subsp paratuberculosis. Afr J Microbiol Res. 2010;4:2787-9.

Sigurðardottir GÓ, Valheim M, Charles MLP. Establishment of Mycobacterium avium subsp. paratuberculosis infection in the intestine of ruminants. Adv Drug Deliv Rev. 2004;56(6):819-34. http://dx.doi.org/10.1016/j.addr.2003.10.032. PMid:15063592.

Stringfellow D, Givens D. Manual of the International Embryo Transfer Society. 4th ed. Champaign: IETS; 2010.

Subharat S, Shu D, Neil Wedlock D, Price-Carter M, Lisle GW, Luo D, Collins DM, Buddle BM. Immune responses associated with progression and control of infection in calves experimentally challenged with Mycobacterium avium subsp. paratuberculosis. Vet Immunol Immunopathol. 2012;149(3-4):225-36. http://dx.doi.org/10.1016/j.vetimm.2012.07.005. PMid:22871577.

Verin R, Perroni M, Rossi G, De Grossi L, Botta R, De Sanctis B, Rocca S, Cubeddu T, Crosby-Durrani H, Taccini E. Paratuberculosis in sheep: Histochemical, immunohistochemical and in situ hybridization evidence of in utero and milk transmission. Res Vet Sci. 2016;106:173-9. http://dx.doi.org/10.1016/j.rvsc.2016.04.006. PMid:27234557.

Wentink GH, Frankena K, Bosch JC, Vandehoek JED, Van Den Berg T. Prevention of disease transmission by semen in cattle. Livest Prod Sci. 2000;62(3):207-20. http://dx.doi.org/10.1016/S0301-6226(99)00158-X.

Whipple DL, Kapke PA, Andersen PR. Comparison of a commercial DNA probe test and three cultivation procedures for detection of Mycobacterium paratuberculosis in bovine feces. J Vet Diagn Invest. 1992;4(1):23-7. http://dx.doi.org/10.1177/104063879200400106. PMid:1554765.

Whittington RJ, Begg DJ, Silva K, Plain KM, Purdie AC. Comparative immunological and microbiological aspects of paratuberculosis as a model mycobacterial infection. Vet Immunol Immunopathol. 2012;148(1-2):29-47. http://dx.doi.org/10.1016/j.vetimm.2011.03.003. PMid:21450348.

Whittington RJ, Windsor PA. In utero infection of cattle with Mycobacterium avium subsp. paratuberculosis: A critical review and meta-analysis. Vet J. 2009;179(1):60-9. http://dx.doi.org/10.1016/j.tvjl.2007.08.023. PMid:17928247.

Windsor PA, Whittington RJ. Evidence for age susceptibility of cattle to Johne’s disease. Vet J. 2010;184(1):37-44. http://dx.doi.org/10.1016/j.tvjl.2009.01.007. PMid:19246220.

Windsor PA. Paratuberculosis in sheep and goats. Vet Microbiol. 2015;181(1-2):161-9. http://dx.doi.org/10.1016/j.vetmic.2015.07.019. PMid:26255556.
 

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