Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.21451/1984-3143-AR2019-0001
Animal Reproduction (AR)
Original Article

Evaluation of the proteomic profiles of ejaculated spermatozoa from Saanen bucks (Capra hircus)

Tatiana Maria Farias Pinto; Raulzito Fernandes Moreira; Maria Nagila Carneiro Matos; Vitória Virginia Magalhães Soares; Mônica Valeria de Almeida Aguiar; Paulo de Tarso Teles Dourado de Aragão; João Garcia Alves Filho; Frederico Bruno Mendes Batista Moreno; Ana Cristina de Oliveira Monteiro-Moreira; Cíntia Renata Rocha Costa; José Luiz de Lima Filho; Angela Maria Xavier Eloy; Rodrigo Maranguape Silva da Cunha

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Abstract

Abstract: The Saanen goat breed has been widely explored in breeding programmes; however, there are few reports about the breed’s genetic and molecular composition. Thus, this study aimed to characterize the proteomic profile of spermatozoa from Saanen breeding goats. Five breeding animals with proven fertility were selected, the spermatozoa were collected, and the protein was extracted. Subsequently, the proteins were separated and analysed by two-dimensional electrophoresis and mass spectrometry; the proteins were then identified with the SwissProt database. A total of 31 proteins involved in reproduction were identified, including binding proteins on spermatozoa for fusion with the egg, acrosomal membrane proteins, metabolic enzymes, heat shock proteins, cytoskeletal proteins and spermatozoa motility proteins. The characterization of such proteins clarifies the molecular mechanisms of spermatogenesis and the modifications that ensure the success of fertilization.

Keywords

saanen, sperm, proteomic profiles

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