Animal Reproduction (AR)
https://animal-reproduction.org/article/doi/10.21451/1984-3143-AR2019-0011
Animal Reproduction (AR)
Original Article

Powdered coconut water (ACP 406®) as an alternative base culture medium for in vitro culture of goat preantral follicles enclosed in ovarian tissue

Olga Juliana Roldan Castañeda; Francisco Léo Nascimento de Aguiar; Naiza Arcângela Ribeiro de Sá; Maria Luana Galdencio dos Santos Morais; Francielli Weber Santos Cibin; Ciro Alexandre Alves Torres; José Ricardo de Figueiredo

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Abstract

Abstract: This study evaluated a powdered coconut water solution (ACP 406®) as a base culture medium on the in vitro survival and development of in situ goat preantral follicles. The ovarian fragments were either immediately fixed in Carnoy solution (non-cultured control) or individually cultured for 2 or 6 days. The following culture media (all containing 100 μg/mL penicillin and 100 μg/mL streptomycin) were evaluated: α-MEM (α-MEM alone, without additional supplementation); α-MEM+ (supplemented α-MEM); ACP (ACP®406 alone); or ACP+ (supplemented ACP®406). Additional supplementation includes: 1.25 mg/mL bovine serum albumin, 10 μg/mL insulin, 5.5 μg/mL transferrin, 5 ng/mL selenium, 2 mM glutamine, and 2 mM hypoxanthine. The endpoints (i) follicular morphology; (ii) development; (iii) estradiol production; and (iv) reactive oxygen species (ROS) were recorded. Data were analyzed using chi-square, Turkey, t-test or One-Way ANOVA. Differences were considered significant when P < 0.05. At day 2 of culture, a greater (P < 0.05) percentage of morphologically normal follicles was observed between ACP+ and ACP treatments. Moreover, at day 2 of culture, no hormonal difference (P < 0.05) was observed between ACP+ and both α-MEM treatments. At day 6 of culture when ACP and α-MEM treatments were compared the percentage of healthy follicles were similar (P > 0.05) among treatments. Overall, all treatments had lower primordial follicles (P < 0.05) accompany by greater developing follicles (P < 0.05) percentages than non-cultured control treatment, indicating primordial follicle activation. However, at day 6 of culture, the percentage of primordial follicle development were similar (P > 0.05) among the treatments. Likewise, no differences (P > 0.05) were observed for ROS production and follicular and oocyte diameters among treatments. Therefore, ACP+ has the equivalent efficiency to MEM+ in maintaining the survival and development of goat preantral follicles, representing an alternative plant-based low-cost culture medium for in vitro culture.

Keywords

ACP, base medium, goat, in vitro culture, preantral follicles

References

Aguiar FLN, Gastal GDA, Ishak GM, Gastal MO, Teixeira DIA, Feugang JM, Figueiredo JR, Gastal EL. Effects of FSH addition to an enriched medium containing insulin and EGF after long-term culture on functionality of equine ovarian biopsy tissue. Theriogenology. 2017b;99:124-33. http://dx.doi.org/10.1016/j.theriogenology.2017.04.045. PMid:28708493.

Aguiar FLN, Lunardi FO, Lima LF, Bruno JB, Alves BG, Magalhães-Padilha DM, Cibin FWS, Berioni L, Apgar GA, Lo Turco EG, Gastal EL, Figueiredo JR. Role of EGF on in situ culture of equine preantral follicles and metabolomics profile. Res Vet Sci. 2017a;115:155-64. http://dx.doi.org/10.1016/j.rvsc.2017.04.001. PMid:28414979.

Aguiar FLN, Lunardi FO, Lima LF, Rocha RMP, Bruno JB, Magalhães-Padilha DM, Cibin FW, Nunes-Pinheiro DC, Gastal MO, Rodrigues APR, Apgar GA, Gastal EL, Figueiredo JR. FSH supplementation to culture medium is beneficial for activation and survival of preantral follicles enclosed in equine ovarian tissue. Theriogenology. 2016;85(6):1106-12. http://dx.doi.org/10.1016/j.theriogenology.2015.11.022. PMid:26723132.

Anckaert E, Mees M, Schiettecatte J, Smitz J. Clinical validation of a fully automated 17β-estradiol and progesterone assay (VIDAS®) for use in monitoring assisted reproduction treatment. Clin Chem Lab Med. 2002;40(8):824-31. http://dx.doi.org/10.1515/CCLM.2002.143. PMid:12392313.

Andrade ER, Amorim CA, Matos MHT, Rodrigues APR, Silva JRV, Dode MAN, Figueiredo JR. Evaluation of saline and coconut water solutions in the preservation of sheep preantral follicles in situ. Small Rumin Res. 2002;43(3):235-43. http://dx.doi.org/10.1016/S0921-4488(02)00018-4.

Andrade PM, Chaves RN, Alves AMCV, Rocha RMP, Lima LF, Carvalho AA, Rodrigues APR, Campello CC, Gastal EL, Figueiredo JR. Effects of α-MEM and TCM-199 culture media and epidermal growth factor on survival and growth of goat and sheep preantral follicles cultured in vitro. Anim Reprod. 2014;11:567-72.

Aroucha EMM, Souza CLM, Aroucha MCM, Vianni R. Características físicas e químicas da água de coco anão verde e anão vermelho em diferentes estádios de maturação. Rev Caatinga. 2005;18:82-7.

Arunakumari G, Shanmugasundaram N, Rao VH. Development of morulae from the oocytes of cultured sheep preantral follicles. Theriogenology. 2010;74(5):884-94. http://dx.doi.org/10.1016/j.theriogenology.2010.04.013. PMid:20615540.

Blume H, Vale Filho VR, Marques AP Jr, Saturnino HM. Avaliação da água de coco na maturação de ovócitos bovinos. Rev Bras Reprod Anim. 1997;21:72-5.

Cadenas J, Leiva-Revilla J, Vieira LA, Apolloni LB, Aguiar FLN, Alves BG, Lobo CH, Rodrigues APR, Apgar GA, Smitz J, Figueiredo JR, Maside C. Caprine ovarian follicle requirements differ between preantral and early antral stages after IVC in medium supplemented with GH and VEGF alone or in combination. Theriogenology. 2017;87:321-32. http://dx.doi.org/10.1016/j.theriogenology.2016.09.008. PMid:27729112.

Cecconi S, Barboni B, Coccia M, Mattioli M. In vitro development of sheep preantral follicles. Biol Reprod. 1999;60(3):594-601. http://dx.doi.org/10.1095/biolreprod60.3.594. PMid:10026104.

Chaves RN, Martins FS, Saraiva MVA, Celestino JJH, Lopes CAP, Correia JC, Verde IB, Matos MH, Báo SN, Name KP, Campello CC, Silva JR, Figueiredo JR. Chilling ovarian fragments during transportation improves viability and growth of goat preantral follicles cultured in vitro. Reprod Fertil Dev. 2008;20(5):640-7. http://dx.doi.org/10.1071/RD07195. PMid:18577361.

Cordeiro MS, Silva EHS, Miranda MS, Biondi FC, Santos SSD, Ohashi OM. The use of coconut water solution (Cocos nucifera) as a holding medium for immature bovine oocytes for in vitro embryo production. Anim Reprod. 2006;3:376-9.

Costa SHF, Santos RR, Rodrigues APR, Silva JRV, Celestino JJH, Matos MHT, Andrade ER, Martins FS, Dantas JK, Ohashi OM, Figueiredo JR. In vitro culture of ovine primordial follicles in media supplemented with coconut water. Anim Reprod. 2006;3:403-9.

Eppig JJ, O’Brien MJ. Development in vitro of mouse oocytes from primordial follicles. Biol Reprod. 1996;54(1):197-207. http://dx.doi.org/10.1095/biolreprod54.1.197. PMid:8838017.

Ferreira ACA, Cadenas J, Sá NAR, Correia HHV, Guerreiro DD, Lobo CH, Alves BG, Maside C, Gastal EL, Rodrigues APR, Figueiredo JR. In vitro culture of isolated preantral and antral follicles of goat using human recombinant FSH: concentration-dependent and stage specific effect. Anim Reprod Sci. 2018;196:120-9. http://dx.doi.org/10.1016/j.anireprosci.2018.07.004. PMid:30049427.

Gomes RG, Lisboa LA, Silva CB, Max MC, Marino PC, Oliveira RL, González SM, Barreiros TRR, Marinho LSR, Seneda MM. Improvement of development of equine preantral follicles after 6 days of in vitro culture with ascorbic acid supplementation. Theriogenology. 2015;84(5):750-5. http://dx.doi.org/10.1016/j.theriogenology.2015.05.006. PMid:26074067.

Gouveia BB, Macedo TJS, Santos JMS, Barberino RS, Menezes VG, Müller MC, Almeida JRGS, Figueiredo JR, Matos MHT. Supplemented base medium containing Amburana cearensis associated with FSH improves in vitro development of isolated goat preantral follicles. Theriogenology. 2016;86(5):1275-84. http://dx.doi.org/10.1016/j.theriogenology.2016.04.068. PMid:27287468.

Gupta PSP, Ramesh HS, Manjunatha BM, Nandi S, Ravindra JP. Production of buffalo embryos using oocytes from in vitro grown preantral follicles. Zygote. 2008;16(1):57-63. http://dx.doi.org/10.1017/S096719940700442X. PMid:18221582.

Haag KT, Magalhães-Padilha DM, Fonseca GR, Wischral A, Gastal MO, King SS, Jones KL, Figueiredo JR, Gastal EL. In vitro culture of equine preantral follicles obtained via the Biopsy Pick-Up method. Theriogenology. 2013;79(6):911-7. http://dx.doi.org/10.1016/j.theriogenology.2013.01.001. PMid:23434205.

Higuchi CM, Maeda Y, Horiuchi T, Yamazaki Y. A simplified method for three-dimensional (3-D) ovarian tissue culture yielding oocytes competent to produce full-term offspring in mice. PLoS One. 2015;10(11):1-19. http://dx.doi.org/10.1371/journal.pone.0143114. PMid:26571501.

Jimenez CR, de Azevedo JL, Silveira RG, Penitente-Filho J, Carrascal-Triana EL, Zolini AM, Araújo VR, Torres CAA. Effects of growth hormone on in situ culture of bovine preantral follicles are dose dependent. Reprod Domest Anim. 2016;51(4):575-84. http://dx.doi.org/10.1111/rda.12722. PMid:27342233.

Leong LP, Shui G. An investigation of antioxidant capacity of fruits in Singapore markets. Food Chem. 2002;76(1):69-75. http://dx.doi.org/10.1016/S0308-8146(01)00251-5.

Lima GL, Costa LLM, Cavalcanti DMLP, Rodrigues CMF, Freire FAM, Fontenele-Neto JD, Silva AR. Short-term storage of canine preantral ovarian follicles using a powdered coconut water (ACP®)-based medium. Theriogenology. 2010;74(1):146-52. http://dx.doi.org/10.1016/j.theriogenology.2010.01.025. PMid:20207405.

Loetchutinat C, Kothan S, Dechsupa S, Meesungnoen J, Jay-Gerin JP, Mankhetkorn S. Spectrofluorometric determination of intracellular levels of reactive oxygen species in drug-sensitive and drug-resistant cancer cells using the 2′,7′-dichlorofluorescein diacetate assay. Radiat Phys Chem. 2005;72(2-3):323-31. http://dx.doi.org/10.1016/j.radphyschem.2004.06.011.

Lunardi FO, Chaves RN, Lima LF, Araújo VR, Brito IR, Souza CEA, Donato MAM, Peixoto CA, Dinnyes A, Campello CC, Figueiredo JR, Rodrigues APR. Vitrified sheep isolated secondary follicles are able to grow and form antrum after a short period of in vitro culture. Cell Tissue Res. 2015;362(1):241-51. http://dx.doi.org/10.1007/s00441-015-2181-0. PMid:25948481.

Magalhães DM, Duarte ABG, Araújo VR, Brito IR, Soares TG, Lima IMT, Lopes CAP, Campello CC, Rodrigues APR, Figueiredo JR. In vitro production of a caprine embryo from a preantral follicle cultured in media supplemented with growth hormone. Theriogenology. 2011;75(1):182-8. http://dx.doi.org/10.1016/j.theriogenology.2010.08.004. PMid:20875671.

Martins FS, Van Den Hurk R, Santos RR, Silva JRV, Matos MHT, Celestino JJH, Rodrigues APR, Pessoa C, Ferreira FVA, Figueiredo JR. Development of goat primordial follicles after in vitro culture of ovarian tissue in Minimal Essential Medium supplemented with coconut water. Anim Reprod. 2005;2:106-13.

Matos MHT, Lima-Verde IB, Luque MCA, Maia JE Jr, Silva JRV, Celestino JJH, Martins FS, Báo SN, Lucci CM, Figueiredo JR. Essential role of follicle stimulating hormone in the maintenance of caprine preantral follicle viability in vitro. Zygote. 2007;15(2):173-82. http://dx.doi.org/10.1017/S0967199407004169. PMid:17462110.

Peng X, Yang M, Wang L, Tong C, Guo Z. In vitro culture of sheep lamb ovarian cortical tissue in a sequential culture medium. J Assist Reprod Genet. 2010;27(5):247-57. http://dx.doi.org/10.1007/s10815-010-9415-6. PMid:20393796.

Rossetto R, Saraiva MVA, Dos Santos RR, Da Silva CMG, Faustino LR, Chaves RN, Brito IR, Rodrigues GQ, Lima IMT, Donato MAM, Peixoto CA, Figueiredo JR. Effect of medium composition on the in vitro culture of bovine pre-antral follicles: morphology and viability do not guarantee functionality. Zygote. 2013;21(2):125-8. http://dx.doi.org/10.1017/S0967199412000044. PMid:22717039.

Sá NAR, Araújo VR, Correia HHV, Ferreira ACA, Guerreiro DD, Sampaio AM, Escobar E, Santos FW, Moura AA, Lôbo CH, Ceccatto VM, Campello CC, Rodrigues APR, Leal-Cardoso JH, Figueiredo JR. Anethole improves the in vitro development of isolated caprine secondary follicles. Theriogenology. 2017;89:226-34. http://dx.doi.org/10.1016/j.theriogenology.2015.12.014. PMid:28043356.

Sá NAR, Bruno JB, Guerreiro DD, Cadenas J, Alves BG, Cibin FWS, Leal-Cardoso JH, Gastal EL, Figueiredo JR. Anethole reduces oxidative stress and improves in vitro survival and activation of primordial follicles. Braz J Med Biol Res. 2018;51(8):e7129. http://dx.doi.org/10.1590/1414-431x20187129. PMid:29846431.

Salgueiro CCM, Nunes JF, Oliveira KPL. Utilização de diluentes à base de água de coco in natura e em pó na inseminação artificial programada de cabras. Rev Bras Reprod Anim. 2002;5:175-82.

Santos RR, Amorim C, Cecconi S, Fassbender M, Imhof M, Lornage J, Paris M, Schoenfeldt V, Martinez-Madrid B. Cryopreservation of ovarian tissue: an emerging technology for female germline preservation of endangered species and breeds. Anim Reprod Sci. 2010;122(3-4):151-63. http://dx.doi.org/10.1016/j.anireprosci.2010.08.010. PMid:20832203.

Santos RR, Tharasanit T, Van Haeften T, Figueiredo JR, Silva JRV, Van Den Hurk R. Vitrification of goat preantral follicles enclosed in ovarian tissue by using conventional and solid-surface vitrification methods. Cell Tissue Res. 2007;327(1):167-76. http://dx.doi.org/10.1007/s00441-006-0240-2. PMid:16937112.

Saraiva MVA, Rossetto R, Brito IR, Celestino JJH, Silva CMG, Faustino LR, Almeida AP, Bruno JB, Magalhães DM, Matos MHT, Campello CC, Figueiredo JR. Dynamic medium produces caprine embryo from preantral follicles grown in vitro. Reprod Sci. 2010;17(12):1135-43. http://dx.doi.org/10.1177/1933719110379269. PMid:20926838.

Serafim MKB, Araújo VR, Silva GM, Duarte ABG, Almeida AP, Chaves RN, Campello CC, Lopes CAP, de Figueiredo JR, Silva LDM. Canine preantral follicles cultured with various concentrations of follicle-stimulating hormone (FSH). Theriogenology. 2010;74(5):749-55. http://dx.doi.org/10.1016/j.theriogenology.2010.03.028. PMid:20537700.

Silva AEF, Cavalcante LF, Rodrigues BA, Rodrigues JL. The influence of powdered coconut water (ACP-318®) in in vitro maturation of canine oocytes. Reprod Domest Anim. 2010;45(6):1042-6. http://dx.doi.org/10.1111/j.1439-0531.2009.01487.x. PMid:19570146.

Silva JRV, Ferreira MAL, Costa SHF, Santos RR, Carvalho FCA, Rodrigues APR, Lucci CM, Báo SN, Figueiredo JR. Degeneration rate of preantral follicles in the ovaries of goats. Small Rumin Res. 2002;43(3):203-9. http://dx.doi.org/10.1016/S0921-4488(02)00017-2.

Silva JRV, Lucci CM, Carvalho FCA, Báo SN, Costa SHF, Santos RR, Figueiredo JR. Effect of coconut water and braun-collins solutions at different temperatures and incubation times on the morphology of goat preantral follicles preserved in vitro. Theriogenology. 2000;54(5):809-22. http://dx.doi.org/10.1016/S0093-691X(00)00392-7. PMid:11101040.

Silva JRV, Van Den Hurk R, Costa SHF, Andrade ER, Nunes APA, Ferreira FVA, Lôbo RNB, Figueiredo JR. Survival and growth of goat primordial follicles after in vitro culture of ovarian cortical slices in media containing coconut water. Anim Reprod Sci. 2004;81(3-4):273-86. http://dx.doi.org/10.1016/j.anireprosci.2003.09.006. PMid:14998653.

Silva JRV, van den Hurk R, Figueiredo JR. Ovarian follicle development in vitro and oocyte competence: advances and challenges for farm animals. Domest Anim Endocrinol. 2016;55:123-35. http://dx.doi.org/10.1016/j.domaniend.2015.12.006. PMid:26836404.

Talebi A, Zavareh S, Kashani MH, Lashgarbluki T, Karimi I. The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles. J Assist Reprod Genet. 2012;29(2):175-83. http://dx.doi.org/10.1007/s10815-011-9706-6. PMid:22231012.

Tang K, Yang WC, Li X, Wu CJ, Sang L, Yang LG. GDF-9 and bFGF enhance the effect of FSH on the survival, activation, and growth of cattle primordial follicles. Anim Reprod Sci. 2012;131(3-4):129-34. http://dx.doi.org/10.1016/j.anireprosci.2012.03.009. PMid:22516229.

Vigliar R, Sdepanian VL, Fagundes-Neto U. Biochemical profile of coconut water from coconut palms planted in an inland region. J Pediatr. 2006;82(4):308-12. http://dx.doi.org/10.2223/JPED.1508. PMid:16912834.

Wandji SA, Sřseň V, Nathanielsz PW, Eppig JJ, Fortune JE. Initiation of growth of baboon primordial follicles in vitro. Hum Reprod. 1997;12(9):1993-2001. http://dx.doi.org/10.1093/humrep/12.9.1993. PMid:9363719.

Wang X, Falcone T, Attaran M, Goldberg JM, Agarwal A, Sharma RK. Vitamin C and vitamin E supplementation reduce oxidative stress-induced embryo toxicity and improve the blastocyst development rate. Fertil Steril. 2002;78(6):1272-7. http://dx.doi.org/10.1016/S0015-0282(02)04236-X. PMid:12477524.

Wu J, Emery BR, Carrell DT. In vitro growth, maturation, fertilization, and embryonic development of oocytes from porcine preantral follicles. Biol Reprod. 2001;64(1):375-81. http://dx.doi.org/10.1095/biolreprod64.1.375. PMid:11133696.

Xu M, Fazleabas AT, Shikanov A, Jackson E, Barrett SL, Hirshfeld-Cytron J, Kiesewetter SE, Shea LD, Woodruff TK. In vitro oocyte maturation and preantral follicle culture from the luteal-phase baboon ovary produce mature oocytes. Biol Reprod. 2011;84(4):689-97. http://dx.doi.org/10.1095/biolreprod.110.088674. PMid:21123815.

Xu M, Kreeger PK, Shea LD, Woodruff TK. Tissue-engineered follicles produce live, fertile offspring. Tissue Eng. 2006;12(10):2739-46. http://dx.doi.org/10.1089/ten.2006.12.2739. PMid:17518643.

Yong JWH, Ge L, Ng YF, Tan SN. The chemical composition and biological properties of coconut (Cocos nucifera L.) Water. Molecules. 2009;14(12):5144-64. http://dx.doi.org/10.3390/molecules14125144. PMid:20032881.
 

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